|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Occult hepatitis B virus infection among Egyptian blood donors显示文摘AIM:To identify blood donors with occult hepatitis B virus(HBV) infection(OBI) to promote safe blood donation.METHODS:Descriptive cross sectional study was conducted on 3167 blood donors negative for hepatitis B surface antigen(HBsAg),hepatitis C antibody(HCV Ab) and human immunodeficiency virus Ab.They were subjected to the detection of alanine aminotransferase(ALT) and aspartate transaminase(AST) and screening for anti-HBV core antibodies(total) by two different techniques;[Monoliza antibodies to hepatitis B core(Anti-HBc) Plus-Bio-Rad] and(ARC-HBc total-ABBOT).Positive samples were subjected to quantitative detection of antibodies to hepatitis B surface(anti-HBs)(ETI-AB-AUK-3,Dia Sorin-Italy).Serum anti-HBs titers > 10 IU/L was considered positive.Quantitative HBV DNA by real time polymerase chain reaction(PCR)(QIAGEN-Germany) with 3.8 IU/mL detection limit was estimated for blood units with negative serum anti-HBs and also for 32 whose anti-HBs serum titers were > 1000 IU/L.Also,265 recipients were included,34 of whom were followed up for 3-6 mo.Recipients were investigated for ALT and AST,HBV serological markers:HBsAg(ETI-MAK-4,Dia Sorin-Italy),anti-HBc,quantitative detection of anti-HBs and HBV-DNA.RESULTS:525/3167(16.6%) of blood units were positive for total anti-HBc,64% of those were antiHBs positive.Confirmation by ARCHITECT anti-HBc assay were carried out for 498/525 anti-HBc positive samples,where 451(90.6%) confirmed positive.Reactivity for anti-HBc was considered confirmed only if two positive results were obtained for each sample,giving an overall prevalence of 451/3167(14.2%) for total anti-HBc.HBV DNA was quantified by real time PCR in 52/303(17.2%) of anti-HBc positive blood donors(viral load range:5 to 3.5 x 105 IU/mL) with a median of 200 IU/mL(mean:1.8 x 104 ± 5.1 x 104 IU/mL).AntiHBc was the only marker in 68.6% of donors.Univariate and multivariate logistic analysis for identifying risk factors associated with anti-HBc and HBV-DNA positivity among blood donors showed that age above thirty and marriage were the most significant risk factors for prediction of anti-HBc positivity with AOR 1.8(1.4-2.4) and 1.4(1.0-1.9) respectively.Other risk factors as gender,history of blood transfusion,diabetes mellitus,frequent injections,tattooing,previous surgery,hospitalization,Bilharziasis or positive family history of HBV or HCV infections were not found to be associated with positive anti-HBc antibodies.Among anti-HBc positive blood donors,age below thirty was the most significant risk factor for prediction of HBV-DNA positivity with AOR 3.8(1.8-7.9).According to HBV-DNA concentration,positive samples were divided in two groups;group one with HBV-DNA ≥ 200 IU/mL(n = 27) and group two with HBV-DNA < 200 IU/mL(n = 26).No significant difference was detected between both groups as regards mean age,gender,liver enzymes or HBV markers.Serological profiles of all followed up blood recipients showed that,all were negative for the studied HBV markers.Also,HBV DNA was not detected among studied recipients,none developed post-transfusion hepatitis(PTH) and the clinical outcome was good.CONCLUSION:OBI is prevalent among blood donors.Nucleic acid amplification/HBV anti core screening should be considered for high risk recipients to eliminate risk of unsafe blood donation. | Zeinab N Said Manal H El Sayed Iman I Salama Enas K Aboel-Magd Magda H Mahmoud Maged El Setouhy Faten Mouftah Manal B Azzab Heidi Goubran Amal Bassili Gamal E Esmat | 2013 | World Journal of Hepatology2013，5，2： | 4 |
| 2 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 3 | Effectiveness of hepatitis B virus vaccination program in Egypt:Multicenter national project显示文摘AIM:To assess the effectiveness of hepatitis B virus(HBV) vaccination program among fully vaccinated children.METHODS:A national community based crosssectional study was carried out in 6 governorates representing Egypt. A total of 3600 children aged from 9 mo to 16 years who were fully vaccinated with HBV vaccine during infancy were recruited. Face to face interviews were carried out and sera were evaluated for hepatitis B surface antigen(HBsA g),anti-HBV core antibodies(total) and quantitative detection of hepatitis B surface antibody using enzyme linked immunoassays techniques. Samples positive to HBs Ag/anti-HBV core antibodies were subjected to quantitative HBV-DNA detection by real time polymerase chain reaction with 3.8 IU/L detection limit. RESULTS:Sero-protection was detected among 2059 children(57.2%) with geometric mean titers 75.4 ± 3.6 IU/L compared to 3.1 ± 2.1 IU/L among nonseroprotected children. Multivariate logistic analysis revealed that older age and female gender were the significant predicting variables for having non seroprotective level,with adjusted odds ratio 3.3,9.1and 14.2 among children aged 5 to < 10,10 to < 15 and ≥ 15 years respectively compared to those < 5 years and 1.1 among girls compared to boys with P < 0.01. HBs Ag was positive in 0.11% and breakthrough infection was 0.36% and 0.39% depending on positivity of anti-HBc and DNA detection respectively. The prevalence of HBV infection was significantly higher among children aged ≥ 7 years(0.59%) compared to 0.07% among younger children with odds ratio equal to 8.4(95%CI:1.1-64.2) and P < 0.01.The prevalence was higher among girls(0.48%) than boys(0.29%) with P > 0.05. C ON C LU S I ON :T he E gy pt ian c ompuls or y H B V vaccination program provides adequate protection. Occult HBV infection exists among apparently healthy vaccinated children. Adherence to infection control measures is mandatory. | Iman I Salama Samia M Sami Zeinab Nabil Ahmed Said Manal H El-Sayed Lobna A El Etreby Thanaa M Rabah Dalia M Elmosalami Amany T Abdel Hamid Somaia I Salama Aida M Abdel Mohsen Hanaa M Emam Safaa M Elserougy Amal I Hassanain Naglaa F Abd Alhalim Fatma A Shaaban Samia A Hemeda Nihad A Ibrahim Ammal M Metwally | 2015 | World Journal of Hepatology2015，7，22： | 2 |
| 4 | Excitotoxicity in the lung:N-methyl-D-aspartate-induced,nitric oxide-dependent,pulmonary edema is attenuated by vasoactive intestinal peptide and by inhibitors of poly(ADP-ribose)polymerase显示文摘 | Said SI Berisha HI Pakbaz H | 1996 | Proc Natl Acad Sci U S A1996，93，10： | 1 |
| 5 | Characterisitcs of flow field and water concentration in a horizontal dedleg 显示文摘 | Habib M A Badr H M Said S A M | 2005 | Heal Mass Transfer2005，41，： | 1 |
| 6 | Upregula-tion of PD-1 expression on HIV-specific CD8 T cells leads to reversible immune dysfunction 显示文摘 | Trautmann L Janbazian L Chomont N Said EA Gimmig S Bessette B Boulassel MR Delwart E Sepulveda H Balderas RS Routy JP Haddad EK Sekaly RP | 2006 | Nat Med2006，12，10： | 1 |
| 7 | Biobur- den-responsive antimicrobial PLGA ultrafine fibers for wound healing显示文摘 | Said S S E1-Halfawy O M E1-Gowelli H M | 2012 | Eur J Pharm Biopharm2012，80，1： | 1 |
| 8 | Sensitive measurement of optical nonlinearities using a single beam 显示文摘 | SHEIK-BAHAE M SAID A A WEI T H | 1990 | Journal of Quantum Electronic1990，26，4： | 1 |
| 9 | Effect of geometry on flow fiehl and oil/water separation in vertical deadlegs 显示文摘 | Habib M A Said S A M Badr H M | 2005 | International Journal for Numerical Meth- ods in Heat & Fluid Flow2005，15，4： | 1 |
| 10 | Acoustic-wave/blade-row interactions establish boundary conditions for unsteady inlet Flows显示文摘 | Sajben M Said H | 2001 | Journal of Propulsion and Power2001，17，5： | 1 |
| 11 | A fuzzy rotor resistance updating scheme for an IFOC induction motor drive显示文摘 | Zidani F Nait Said M S Benbouzid M E H | 2001 | IEEE Power Engineering Review2001，21，11： | 1 |
| 12 | First reported cases of intravascular large cell lymphoma of the NK cell type:clinical,histologic,immunophenotypic,and molecular features显示文摘 | Wu H Said JW Ames ED | 2005 | Am J Clin Pathol2005，123，4： | 1 |
| 13 | The relationship be- tween corporate social responsibility disclosure and cor- porate governance characteristics in Malaysian public listed companies 显示文摘 | Said R Zainuddin Y H Haron H | 2009 | Social Responsibility Journcol2009，5，2： | 1 |
| 14 | 显示文摘 | BaHae M S Said A A Wei T H | 1990 | IEEE J Quantum Electron1990，26，： | 1 |
| 15 | Adaptive Logical Framework for Integrated Flood Management A- long Sarawak River 显示文摘 | POON H PUTUHENA F SAID SALIM B | 2009 | International Journal of Hydro- Climatic Engineering2009，1，1： | 1 |
| 16 | Generalized Ball curve and its recursive algorithm 显示文摘 | SAID H B | 1989 | ACM Transactions on Graphics1989，8，4： | 1 |
| 17 | Profile of cytokine production within the peripara-sitic granuloma in human alveolar echinococcos显示文摘 | Said H Veronique G Solange B | 2003 | Acta Trop2003，85，2： | 1 |
| 18 | Abrogation of transforming growth factor-beta signaling by SMAD7 inhibits collagen gel contraction of human dermal fibroblasts显示文摘 | KoppJ Preis E Said H | 2005 | J Bioi Chern2005，280，21： | 1 |
| 19 | 显示文摘 | Sheik-Bahea M Said A A Wei T H | 1990 | IEEE J Quant Electron1990，26，4： | 1 |
| 20 | Theoretical study of lateral reaction mechanism of piles显示文摘 | BAGUELIN F FRANK R SAID R H | | 0，，03： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员