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| 1 | Full-thickness tissue engineered skin constructed with autogenic bone marrow mesenchymal stem cells显示文摘To explore the feasibility of repairing clinical cutaneous deficiency, autogenic bone marrow mesen-chymal stem cells (BMSCs) were isolated and differentiated into epidermal cells and fibroblasts in vitro supplemented with different inducing factors and biomaterials to construct functional tissue- engineered skin. The results showed that after 72 h induction, BMSCs displayed morphologic changes such as typical epidermal cell arrangement, from spindle shape to round or oval; tonofibrils, melano-somes and keratohyaline granules were observed under a transmission electronic microscope. The differentiated cells expressed epidermal stem cell surface marker CK19 (59.66% ± 4.2%) and epidermal cells differentiation marker CK10. In addition, the induced epidermal cells acquired the anti-radiation capacity featured by lowered apoptosis following exposure to UVB. On the other hand, the collagen microfibrils deposition was noticed under a transmission electronic microscope after differentiating into dermis fibroblasts; RT-PCR identified collagen type I mRNA expression in differentiated cells; radioimmunoassay detected the secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8) (up to 115.06 pg/mL and 0.84 ng/mL, respectively). Further in vivo implanting BMSCs with scaffold material short-ened skin wound repair significantly. In one word, autogenic BMSCs have the potential to differentiate into epidermal cells and fibroblasts in vitro, and show clinical feasibility acting as epidermis-like and dermis-like seed cells in skin engineering. | HE LiJuan, NAN Xue, WANG YunFang, GUAN LiDong, BAI CiXian, SHI ShuangShuang, YUAN HongFeng, CHEN Lin, LIU DaQing & PEI XueTao Laboratory of Stem Cells and Regenerative Medicine, Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China | 2007 | Science China(Life Sciences)2007，50，4： | 16 |
| 2 | Autocrine production of interleukin 6 causes multidrug resistance in breast cancer cells 显示文摘 | Conze D Weiss L Regen PS l | 2001 | Cancer Res2001，61，24： | 1 |
| 3 | Latest Technology Equidistant Power Section Increases Overall Performance of a Workover Motor显示文摘 | Frans Hazenberg Thorsten Regener | 2002 | SPE 74825 -MS2002，，： | 1 |
| 4 | Reduced amygdala volume in newly admitted psychiatric in-patients with unipolar major depression显示文摘 | Kronenberg G Tebartz van Elst L Regen F | 2009 | J Psychiatr Res2009，43，13： | 1 |
| 5 | Computational microstructure analyzing technique for quantitative characterization of shrinkage and gas pores in pressure die cast AZ91 magnesium alloy 显示文摘 | LEO PRAKASH D G PRASANNA B REGENER D | 2005 | Computational Materials Science2005，，32： | 1 |
| 6 | Dendrimers as bluilding block for multilayer construction 显示文摘 | Walanabe S J Regen S L | 1994 | J Am Chem Soc1994，116，88： | 1 |
| 7 | Computational micmstructure analyzing lechnique for quantitative characterization of shrinkage and gas pores in pressure die cast AZ91 Magnesium alloy显示文摘 | LEO PRAKASH D G PRASANNA B REGENER D | 2005 | Computational Materials Science2005，，32： | 1 |
| 8 | On a sensitive method for the recording of atmospheric ozone显示文摘 | Regener V H | 1960 | Journal of Geophysical Re- search1960，65，12： | 1 |
| 9 | Perspectives on valuing forest ecosystemheslth显示文摘 | Regens J L Hodges D G | 1996 | Ecosystem Health1996，22，36： | 1 |
| 10 | Activation through impregnation, permanganate-coated solid supports显示文摘 | Regen S L Koteel C | 1977 | J Am Chem Soc1977，99，： | 1 |
| 11 | Modification of low density polyethylene film using polymerizable surfactants显示文摘 | REGEN J L | 1985 | Macromolecules1985，18，7： | 1 |
| 12 | BDNF promotes not survival, of injured sero tonergic axons in the adult rat brain显示文摘 | Mamouns LA Altar CA the regenerative sprouting Blue but ME | 2000 | Neurosci2000，20，2： | 1 |
| 13 | Solid-phase cosolvents:Tri-phase catalytic hydrolysis of 1-bromoadamantane显示文摘 | Regen S L Besse J J Mclick J | 1979 | Journal of the American Chemical Society1979，101，： | 1 |
| 14 | A school outbreak of Norwalklike virus: evidence for airborne transmission显示文摘 | Marks PJ Vi pond 1B Regen FM | 2003 | Epidemiology and Infection2003，131，： | 1 |
| 15 | Methodological issues in valuing forest ecosystem health显示文摘 | Hodges D G Regens J L | 1996 | Ecosystem Health1996，2，1： | 1 |
| 16 | Total synthesis of papulacandin D显示文摘 | Denmark S E Regens C S Kobayashi T | 2007 | J Am Chem Soc2007，129，： | 1 |
| 17 | Polymerized vesicles 显示文摘 | Regen S L Czech B Singh A | 1980 | J Am Chem Soc1980，102，： | 1 |
| 18 | Increased EEG sigma and beta power during NREM sleep in primary insomnia显示文摘 | Spiegelhald~r K Regen W Feige B | 2012 | BioI Psychol2012，91，3： | 1 |
| 19 | Polymerizable ion-paired amphiphiles 显示文摘 | HIRANO K FUKUDA H REGEN S L | 1991 | Langmuir1991，7，6： | 1 |
| 20 | A comparison of aluminumbasedmet-al-matrix composites reinforced with coated and uncoated particulate silicon carbide 显示文摘 | Davidson A M Regener D | 2000 | Composites Science and Technology2000，60，： | 1 |
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