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| 1 | Attenuation of dextran sodium sulphate induced colitis in matrix metalloproteinase-9 deficient mice显示文摘AIM: To study whether matrix metalloproteinase-9 (MMP-9) is a key factor in epithelial damage in the dextran sodium sulphate (DSS) model of colitis in mice.METHODS: MMP-9-deficient and wild-type (wt) mice were given 5% DSS in drinking water for 5 d followed by recovery up to 7 d. On d 5 and 12 after induction of colitis, gelatinases, MMP-2 and MMP-9, were measured in homogenates of colonic tissue by zymography and Western blot, whereas tissue inhibitor of metalloproteinases (TIMPs) were measured by reverse zymography. The gelatinolytic activity was also determined in supernatants of polymorphonuclear leukocytes (PMN) isolated from mice blood. Moreover, intestinal epithelial cells were stimulated with TNF-α to study whether these cells were able to produce MMPs. Finally, colonic mucosal lesions were measured by microscopic examination. RESULTS: On d 5 of colitis, the activity of MMP-9 was increased in homogenates of colonic tissues (0.24 ± 0.1 vs 21.3 ± 6.4, P < 0.05) and PMN from peripheral blood in wt (0.5 ± 0.1 vs 10.4 ± 0.7, P < 0.05), but not in MMP-9-deficient animals. The MMP-9 activity was also up-regulated by TNF-α in epithelial intestinal cells (2.5 ± 0.5 vs 14.7 ± 3.0, P < 0.05). Although colitis also led to increase of TIMP-1 activity, the MMP-9/TIMP-1 balance remained elevated. Finally, in the MMP-9-deficient colitic mice both the extent and severity of intestinal epithelialinjury were significantly attenuated when compared with wt mice. CONCLUSION: We conclude that DSS induced colitis is markedly attenuated in animals lacking MMP-9. This suggests that intestinal injury induced by DSS is modu-lated by MMP-9 and that inhibition of this gelatinase may reduce inflammation. | Alfredo Santana Carlos Medina Maria Cristina Paz-Cabrera Federico Díaz-Gonzalez Esther Farré Antonio Salas Marek W Radomski Enrique Quintero | 2006 | World Journal of Gastroenterology2006，12，40： | 9 |
| 2 | Phagocytic and oxidative burst activity of neutrophils in the end stage of liver cirrhosis显示文摘AIM: To evaluate the phagocytic activity and neutrophil oxidative burst in liver cirrhosis.METHODS: In 45 patients with advanced postalcoholic liver cirrhosis (aged 45±14 years) and in 25 healthy volunteers (aged 38±5 years), the percentage of phagocytizing cells after in vitro incubation with E. coli (Phagotest Kit), phagocytic activity (mean intensity of fluorescence, MIF) and the percentage of neutrophil oxidative burst (Bursttest Kit), and the level of free oxygen radical production (MIF of Rodamine 123)were analyzed by flow cytometry. The levels of soluble sICAM-1, sVCAM-1, sP-selectin, sE-selectin, sL-selectin,and TNF-α were determined in blood serum.RESULTS: The percentage of E. coli phagocytizing neutrophils in liver cirrhosis patients was comparable to that in healthy subjects. MIF of neutrophil - ingested E. coli was higher in patients with liver cirrhosis. The oxidative burst in E. coli phagocytizing neutrophils generated less amount of active oxygen compounds in liver cirrhosis patients (MIF of R123:24.7±7.1 and 29.7±6.6 in healthy,P<0.01). Phorbol myristate acetate (PMA) - stimulated neutrophilsproduced less reactive oxidants in liver cirrhosis patients than in healthy subjects (MIF of R123: 42.7±14.6 vs 50.2±13.3, P<0.01). A negative correlation was observed between oxidative burst MIF of PMA-stimulated neutrophils and ALT and AST levels (r -0.35, P<0.05;r-0.4, P<0.03). sVCAM-1, sICAM-1, sE-selectin concentrations correlated negatively with the oxygen free radical production (MIF of R123) in neutrophils after PMA stimulation in liver cirrhosis patients (r-0.45, P<0.05;r-0.41, P<0.05; r-0.39, P<0.05, respectively).CONCLUSION: Neutrophil metabolic activity diminishes together with the intensification of liver failure. The metabolic potential of phagocytizing neutrophils is significantly lower in liver cirrhosis patients, which can be one of the causes of immune mechanism damage. The evaluation of oxygen metabolism of E. coli-stimulated neutrophils reveals that the amount of released oxygen metabolites is smaller in liver cirrhosis patients than in healthy subjects. | Anatol Panasiuk Jolanta Wysocka Elzbieta Maciorkowska Bozena Panasiuk Danuta Prokopowicz Janusz Zak Karol Radomski | 2005 | World Journal of Gastroenterology2005，11，48： | 4 |
| 3 | Potential role of fibroblast growth factor in enhancement of fracture healing显示文摘 | Radomsky M L Thompson A Y Spiro R C | 1998 | Clin Orthop1998，，355： | 2 |
| 4 | Increasing Rail Life by Forecasting Fatigue Failure显示文摘 | Massel A Radomski R | 2003 | NDR&E International2003，36，： | 1 |
| 5 | Novel formulation of fibroblast growth factor-2 in a hyaluronan gel accelerates fracture healing in nonhuman primates显示文摘 | Aufdemorte TB Swain LD | 1999 | J Orthop Res1999，17，4： | 1 |
| 6 | The value of immediate or early catheterization of the traumatized posterior urethra显示文摘 | Thijssen A Radomski SB | 1992 | J Urol1992，148，： | 1 |
| 7 | The role of nitric oxide and metalloproteinases in the pathogenesis of hyperoxia - induced lung injury in neonatal rat显示文摘 | Radomski A Sawicki G Olson DM | 1998 | Br J Pharmacol1998，125，7： | 1 |
| 8 | The role of nitric oxide and metalloproteinases in the pathogenesis of hyperoxia-induced lung injury in neonate rat显示文摘 | Radomski A Sawicki G Olson DM | 1998 | Br J Pharmacol1998，125，7： | 1 |
| 9 | Long-term dox- ycycline is more effective than atenolol to prevent thoracic aortic aneurysm in marfan syndrome through the inhibition of matrix metalloproteinase-2 and-9显示文摘 | Chung AW Yang HH Radomski MW | 2008 | Circ Res2008，102，8： | 1 |
| 10 | An exploratory approach to the serotonin syndrome: an update of clinical phenomenology and revised diagnostic criteria 显示文摘 | Radomski JW Dursun SM Revely MA | 2000 | Med Hypotheses2000，55，3： | 1 |
| 11 | The role of nitric oxide and metallopro-teinases in the pathogenesis of hyperoxia-induced lung injury in newbom rats显示文摘 | Radomski A Sawicki G Olson DM | 1998 | Br J Pharmacol1998，125，7： | 1 |
| 12 | Selectins- potential pharmacological targets? 显示文摘 | Kneuer C Ehrhardt C Radomski MW | 2006 | Drug Discov Today2006，11，2122： | 1 |
| 13 | Safety behaviour enhances the acceptability of exposure显示文摘 | Levy HC Radomsky AS | 2014 | Cogn Behav Ther2014，43，1： | 1 |
| 14 | New insights into the butyric acid metabolism of Clostridium acetobutylicum 显示文摘 | Lehmann D Radomski N Ltitke-Eversloh T | 2012 | Appl Microbiol Biotechno12012，96，： | 1 |
| 15 | A model of influenza virus spread as a function of temperature and humidity 显示文摘 | Zuk T Rakowski F Radomski J P | 2009 | Computational Biology and Chemistry2009，33，2： | 1 |
| 16 | The nitric oxide-endothelin-1 connection显示文摘 | Alonso D Radomski MW | 2003 | Heart Fail Rev2003，8，1： | 1 |
| 17 | Probabilistic model of influenza virus transmissibility at various temperature and humidity conditions 显示文摘 | Zuk T Rakowski F Radomski J P | 2009 | Computational Biology and Chemistry2009，33，4： | 1 |
| 18 | Flyroek Phenomena and Area Security in Blasting-related Accidents显示文摘 | Kecojevic V Radomsky M | 2005 | Safety Sci- ence2005，43，： | 1 |
| 19 | Potential role of fibroblast growth factor in enhancement of fracture Healing 显示文摘 | Radomsky ML Thompson AY | 1998 | Clin Orthop1998，355，： | 1 |
| 20 | An L-arginine nitric oxide pathway present in human platelets regulates aggregation 显示文摘 | Radomski MW Palmer RMJ Moncade S | 1990 | Proc Nat! Acad Sci USA1990，87，9： | 1 |
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