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| 1 | NK cell-based immunotherapy for malignant diseases显示文摘生来的杀手(NK ) 房间对癌症在主人免疫起关键作用。在反应,癌症开发机制逃离 NK 房间攻击或导致有缺点的 NK 房间。当前的 NK 基于房间的癌症免疫疗法试图用几条途径克服 NK 房间麻痹。一条途径使用扩展 allogeneic NK 房间,它没被象自体同源的 NK 房间一样的自我 histocompatibility 抗原禁止,为采纳细胞的免疫疗法。另一条采纳转移途径使用稳定的 allogeneic NK 房间线,它为质量控制和大规模生产是更实际的。第三条途径是新鲜 NK 房间或 NK 房间线的基因修正高度表示 cytokines, Fc 受体或妄想的肿瘤抗原受体。治疗学的 NK 细胞能从各种各样的来源被导出,包括细胞,干细胞或甚至导致的 pluripotent 干细胞( iPSCs ),和许多激发器能被用于的外设或绳索血在实验室或好生产实践( GMP )的大规模生产设备包括可溶的生长因素,使不能调动的分子或抗体,和另外的细胞的使活跃之物。到在临床的试用的癌症的对待几类型的 NK 房间治疗的一张表这里被考察。基于 NK 的免疫疗法的几条不同途径例如织物特定的 NK 房间,漂亮面向受体的 NK 房间和化学上对待的 NK 房间,被讨论。一些新技术或策略到由非侵略的成像的监视器 NK 房间治疗,预定 NK 房间治疗由的效率在 vivo 实验并且在临床的试用评估 NK 房间治疗途径也被介绍。 | Min Cheng Yongyan Chen Weihua Xiao Rui Sun Zhigang Tian | 2013 | Cellular & Molecular Immunology2013，10，3： | 61 |
| 2 | Network Pharmacology Bridges Traditional Application and Modern Development of Traditional Chinese Medicine显示文摘Traditional Chinese medicine(TCM) has developed over thousands of years and has accumulated abundant clinical experience, forming a comprehensive and unique medical system. Emerging evidence has begun to illustrate TCM as an area of important medical rediscoveries. This review article briefly introduced the concept, significance,and technology of network pharmacology based on network biology and systems biology. It focused on the theoretical system and potential prospect of TCM network applied in TCM research and development including predicting new drug targets, action mechanism, new drug discovery; evaluating pharmacodynamics, pharmacokinetics, safety, toxicology, quality control, and bioinformatics of drugs. We also discussed the opportunities and challenges in the development and application of network pharmacology in the modernization of TCM research. | Chang-xiao Liu Rui Liu Hui-rong Fan Xue-feng Xiao Xiao-peng Chen Hai-yu Xu Yan-ping Lin | 2015 | Chinese Herbal Medicines2015，7，1： | 78 |
| 3 | A comprehensive classification method for VOC emission sources to tackle air pollution based on VOC species reactivity and emission amounts显示文摘In China, volatile organic compound(VOC) control directives have been continuously released and implemented for important sources and regions to tackle air pollution. The corresponding control requirements were based on VOC emission amounts(EA), but never considered the significant differentiation of VOC species in terms of atmospheric chemical reactivity. This will adversely influence the effect of VOC reduction on air quality improvement. Therefore,this study attempted to develop a comprehensive classification method for typical VOC sources in the Beijing–Tianjin–Hebei region(BTH), by combining the VOC emission amounts with the chemical reactivities of VOC species. Firstly, we obtained the VOC chemical profiles by measuring 5 key sources in the BTH region and referencing another 10 key sources, and estimated the ozone formation potential(OFP) per ton VOC emission for these sources by using the maximum incremental reactivity(MIR) index as the characteristic of source reactivity(SR). Then, we applied the data normalization method to respectively convert EA and SR to normalized EA(NEA) and normalized SR(NSR) for various sources in the BTH region.Finally, the control index(CI) was calculated, and these sources were further classified into four grades based on the normalized CI(NCI). The study results showed that in the BTH region,furniture coating, automobile coating, and road vehicles are characterized by high NCI and need to be given more attention; however, the petro-chemical industry, which was designated as an important control source by air quality managers, has a lower NCI. | Guohao Li Wei Wei Xia Shao Lei Nie Hailin Wang Xiao Yan Rui Zhang | 2018 | Journal of Environmental Sciences2018，30，5： | 32 |
| 4 | The altered expression of inflammation-related microRNAs with microRNA-155 expression correlates with Th17 differentiation in patients with acute coronary syndrome显示文摘MicroRNAs(miRNAs)are a novel class of small,non-coding RNAs that play a significant role in both inflammatory and cardiovascular diseases.Immune cells,especially T helper(Th)cells,are critical in the development of atherosclerosis and the onset of acute coronary syndrome(ACS).To assess whether inflammation-related miRNAs(such as miR-155,146a,21,125a-5p,125b,31)are involved in the imbalance of Th cell subsets in patients with ACS,we measured the expression of related miRNAs in patients with acute myocardial infarction(AMI),unstable angina(UA),stable angina(SA)and chest pain syndrome(CPS);analyzed the relationship between miRNA expression and the frequency of Th cell subsets;and observed the co-expression of miR-155 and IL-17A in peripheral blood mononuclear cells(PBMCs)of patients with ACS.The results showed that the expression of miR-155 in the PBMCs of patients with ACS was decreased by approximately 60%,while the expression of both miR-21 and miR-146a was increased by approximately twofold.The expression patterns of miRNAs in plasma correlated with those in PBMCs,except for miR-21,which was increased by approximately sixfold in the AMI group and showed no significant difference between the UA group and the CPS group.We also found that the expression of miR-155 inversely correlated with the frequency of Th17 cells(r520.896,P,0.01)and that miR-155 was co-expressed with IL-17A in patients with ACS.In conclusion,our study revealed the expression patterns of inflammation-related miRNAs in patients with ACS and found that miR-155 may be associated with Th17 cell differentiation. | Rui Yao Yulan Ma Youyou Du Mengyang Liao Huanhuan Li Wei Liang Jing Yuan Zhijun Ma Xian Yu Hong Xiao Yuhua Liao | 2011 | Cellular & Molecular Immunology2011，8，6： | 32 |
| 5 | Changes of Urban Wetland Landscape Pattern and Impacts of Urbanization on Wetland in Wuhan City显示文摘In this study,remote sensing data of Wuhan City,Hubei Province,China in 1996-2001 were selected to ex-tract wetland landscape information.Several landscape indices were used to evaluate the changes of landscape pattern within the five years,including patch number,patch density,patch fractal dimension,landscape diversity,dominance,evenness,and fragmentation indexes.Then,transformation probabilities of wetland landscapes into non-wetland land-scapes were calculated based on Markov Model,and on these grounds the relationship between changes of wetland landscape pattern and urban construction was analyzed.The results showed that fragmentation degree of all wetland types increased,lake area declined,and dominance of natural wetland decreased.The reasons for these results were mainly because of urban construction.According to the features of abundant wetland in Wuhan City,we suggested that protection of wetland landscape should cooperate with urban construction,which means wetland should become im-portant part of urban landscape. | WANG Xuelei NING Longmei YU Jing XIAO Rui LI Tao | 2008 | Chinese Geographical Science2008，18，1： | 27 |
| 6 | Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity显示文摘感觉神经原被不同发信号的网络和接受特征区分。因此,感觉神经原类型能被连接基于 transcriptome 的神经原与感觉显型打字定义。这里,我们分类老鼠的 somatosensory 神经原由高范围的单个房间的定序 RNA 的背面的根中心(DRG )(10 950 ±1 218 基因每神经原) 并且神经原基于尺寸的层次聚类。而且,对皮肤的刺激作出回应的单个 DRG 神经原被记录用一在里面 vivo 整个房间的补丁夹钳技术并且由神经原类型分类基因标记。小直径 DRG 神经原被分类进低阀值的 mechanoreceptor 的一种类型和 mechanoheat nociceptors (MHN ) 的五种类型。每 MHN 类型进一步被分成二种子类型。大 DRG 神经原被分成四种类型,包括表示联系 BAI1 的蛋白质 2-like 的 neurexophilin 1 快车 MHN 和机械 nociceptors (MN ) 1 (Baiap2l1 ) 。表示 trafficking 蛋白质粒子建筑群 3-like 和 Baiap2l1 显著的 MN 的 Mechanoreceptors 各被细分进二种子类型。这些结果为编目 somatosensory 神经原和他们的 transcriptome 数据库提供一个新系统。 | Chang-Lin Li Kai-Cheng Li Dan Wu Yan Chen Hao Luo Jing-Rong Zhao Sa-Shuang Wang Ming-Ming Sun Ying-Jin Lu Yan-Qing Zhong Xu-Ye Hu Rui Hou Bei-Bei Zhou Lan Bao Hua-Sheng Xiao Xu Zhang | 2016 | Cell Research2016，26，1： | 21 |
| 7 | Anatomy of composition and nature of plate convergence: Insights for alternative thoughts for terminal India-Eurasia collision显示文摘The pattern and timing of collision between India and Eurasia have long been a major concern of the international community. However, no consensus has been reached hitherto. To explore and resolve the disagreements in the Himalayan study,in this paper we begin with the methodology and basic principles for the anatomy of composition and nature of convergent margins,then followed by an effort to conduct a similar anatomy for the India-Eurasia collision. One of the most common patterns of plate convergence involves a passive continental margin, an active continental margin and intra-oceanic basins together with accreted terranes in between. The ultimate configuration and location of the terminal suture zone are controlled by the basal surface of the accretionary wedge, which may show fairly complex morphology with Z-shape and fluctuant geometry. One plausible method to determine the terminal suture zone is to dissect the compositions and structures of active continental margins. It requires a focus on various tectonic elements belonging to the upper plate, such as accretionary wedges, high-pressure(HP)-ultra-high-pressure(UHP) metamorphic rocks, Barrovian-type metamorphic rocks and basement nappes, together with superimposed forearc basins.Such geological records can define the extreme limits and the intervening surface separating active margin from the passive one,thus offering a general sketch for the surface trace of the terminal suture zone often with a cryptic feature. Furthermore, the occurrence of the cryptic suture zone in depth may be constrained by geophysical data, which, in combination with outcrop studies of HP-UHP metamorphic rocks, enables us to outline the terminal suture zone. The southern part of the Himalayan orogen records complicated temporal and spatial features, which are hard to be fully explained by the classic 'two-plate-one-ocean' template,therefore re-anatomy of the compositions and nature for this region is necessitated. Taking advantage of the methodology and basic principles of plate convergence anatomy and synthesizing previous studies together with our recent research, we may gain new insights into the evolution of the Himalayan orogeny.(1) The Yarlung-Zangbo ophiolite is composed of multiple tectonic units rather than a single terminal suture zone, and a group of different tectonic units were juxtaposed against each other in the backstop of the Gangdese forearc.(2) The Tethyan Himalayan Sequence(THS) contains mélanges with typical block-in-matrix structures, uniform southwards paleocurrents and age spectra of detrital zircons typical of Eurasia continent. All of these facts indicate that the THS belonged to Eurasia plate before the terminal collision, emplaced in the forearc of the Gangdese arc.(3) The Greater Himalayan Crystalline Complex(GHC) and Lesser Himalayan Sequence(LHS) comprise complex components including eclogites emplaced into the GHC and the upper part of the LHS. Judging from the fact that HP-UHP metamorphic rocks are exhumed and emplaced in the upper plate, the GHC and the upper part of the LHS where eclogite occur should be assigned to the upper plate, lying above the terminal subduction zone surface. It is the very surface along which the continuous subduction of the India subcontinent occurred, therefore acting as the terminal, cryptic suture. From the suture further southward, the bulk rock associations of the LHS and Sub-Himalayan Sequence(Siwalik) show little affinity of mélange, probably belonging to the foreland system of the India plate. By the anatomy of tectonic features of all the tectonic units in the Himalayan orogen as well as the ages of the subduction-accretion related deformation, we conclude that the terminal India-Eurasia collision occurred after 14 Ma, the timing of the metamorphism of the eclogites emplaced into the upper plate. The development of rifts stretching in N-S direction in Tibet and tectonic events with the transition from sinistral to dextral movements in shear zones, such as the Ailaoshan fault in East Tibet, can coordinately reflect the scale and geodynamic influence of the India-Eurasia convergence zone.By conducting a detailed anatomy of the southern Himalayas, we propose a new model for the final collision-accretion of the Himalayan orogeny. Our study indicates that the anatomy of structures, composition, and tectonic nature is the key to a better understanding of orogenic belts, which may apply to all the orogenic belts around the world. We also point out that several important issues regarding the detailed anatomy of the structures, compositions and tectonic nature of the Himalayan orogeny in future. | XIAO WenJiao AO SongJian YANG Lei HAN ChunMing WAN Bo ZHANG Ji'En ZHANG ZhiYong LI Rui CHEN ZhenYu SONG ShuaiHua | 2017 | Science China Earth Sciences2017，60，6： | 20 |
| 8 | Growth factors-based therapeutic strategies and their underlying signaling mechanisms for peripheral nerve regeneration显示文摘Peripheral nerve injury(PNI),one of the most common concerns following trauma,can result in a significant loss of sensory or motor function.Restoration of the injured nerves requires a complex cellular and molecular response to rebuild the functional axons so that they can accurately connect with their original targets.However,there is no optimized therapy for complete recovery after PNI.Supplementation with exogenous growth factors(GFs)is an emerging and versatile therapeutic strategy for promoting nerve regeneration and functional recovery.GFs activate the downstream targets of various signaling cascades through binding with their corresponding receptors to exert their multiple effects on neurorestoration and tissue regeneration.However,the simple administration of GFs is insufficient for reconstructing PNI due to their short half‐life and rapid deactivation in body fluids.To overcome these shortcomings,several nerve conduits derived from biological tissue or synthetic materials have been developed.Their good biocompatibility and biofunctionality made them a suitable vehicle for the delivery of multiple GFs to support peripheral nerve regeneration.After repairing nerve defects,the controlled release of GFs from the conduit structures is able to continuously improve axonal regeneration and functional outcome.Thus,therapies with growth factor(GF)delivery systems have received increasing attention in recent years.Here,we mainly review the therapeutic capacity of GFs and their incorporation into nerve guides for repairing PNI.In addition,the possible receptors and signaling mechanisms of the GF family exerting their biological effects are also emphasized. | Rui Li Duo-hui Li Hong-yu Zhang Jian Wang Xiao-kun Li Jian Xiao | 2020 | Acta Pharmacologica Sinica2020，41，10： | 19 |
| 9 | Collision Tectonics between the Tarim Block(Basin)and the Northwestern Tibet Plateau:New Observations from a Multidisciplinary Geoscientific Investigation in the Western Kunlun Mountains显示文摘New results from deep seismic reflection profiling, wide-angle reflection-refraction profiling and broadband seismic experiments reveal that a series of south-dipping reflectors occur on the southern margin of the Tarim block (basin). However, it is these south-dipping structures that are intercepted by another series of north-dipping reflectors at depths from 30 to about 150 km beneath the foreland of the W Kunlun Mountains. No evidence from the above geophysical data as well as geochemical and surface geological data indicate the southward subduction of the Tarim block beneath the W Kunlun Mountains (NW Tibet plateau), forming the so-called “two-sided subduction” model for the Tibet plateau as proposed by previous studies. So the authors infer that the tectonic interaction between the Tarim block and the W Kunlun block was chiefly affected by a “horizontal compression in opposite directions”, which brought about “face-to-face contact” between these two lithospheric blocks and led to the thickening, shortening and densifying of the lithosphere. Hence a “delamination” was formed due to the gravitational instability created by the thickening and densifying; then alkaline basic volcanic rocks (mainly shoshonite series) was erupted along the northern margin of the Tibet plateau owing to the delamination. This inference for the formation of the alkaline basic volcanics has been confirmed by recent geochemical and petrological studies in Tibet, indicating that different contacts control different magmatic activities: the alkali basalts are always developed in the “horizontal shortening boundary (contact)” on the northern margin of the Tibet plateau, while the muscovite granite and two-mica granite (leucogranite) in the “subductional contact” on the southern margin of the Tibet plateau. | XIAO Xuchang LIU Xun GAO Rui Houn KAO LUO Zhaohua | 2001 | Acta Geologica Sinica(English Edition)2001，75，2： | 19 |
| 10 | Survival of transplanted neurotrophin-3 expressing human neural stem cells and motor function in a rat model of spinal cord injury显示文摘<正>BACKGROUND:Many methods have been attempted to repair nerves following spinal cord injury, including peripheral nerve transplantation,Schwann cell transplantation,olfactory ensheathing cell transplantation,and embryonic neural tissue transplantation.However,there is a need for improved outcomes. OBJECTIVE:To investigate the repair feasibility for rat spinal cord injury using human neural stem cells(hNSCs) genetically modified by lentivirus to express neurotrophin-3. DESIGN,TIME AND SETTING:In vitro cell biological experiment and in vivo randomized,controlled, genetic engineering experiment were performed at the Third Military Medical University of Chinese PLA and First People's Hospital of Yibin,China from March 2006 to December 2007. MATERIALS:A total of 64 adult,female,Wistar rats were used for the in vivo study.Of them,48 rats were used to establish models of spinal cord hemisection,and were subsequently equally and randomly assigned to model,genetically modified hNSC,and normal hNSC groups.The remaining 16 rats served as normal controls. METHODS:hNSCs were in vitro genetically modified by lentivirus to secrete both green fluorescence protein and neurotrophin-3.Neurotrophin-3 expression was measured by Western blot. Genetically modified hNSC or normal hNSC suspension(5×10~5) was injected into the rat spinal cord following T_(10) spinal cord hemisection.A total of 5μL Dulbecco's-modified Eagle's medium was infused into the rat spinal cord in the model group.Transgene expression and survival of transplanted hNSCs were determined by immunohistochemistry.Motor function was evaluated using the Basso,Beattie,and Bresnahan(BBB) scale. MAIN OUTCOME MEASURES:The following parameters were measured:expression of neurotrophin-3 produced by genetically modified hNSCs,transgene expression and survival of hNSCs in rats,motor function in rats. RESULTS:hNSCs were successfully genetically modified by lentivirus to stably express neurotrophin-3.The transplanted hNSCs primarily gathered at,or around,the injection site two weeks following transplantation,and gradually migrated towards the surrounding tissue. Transplanted hNSCs were observed 7.0-8.0 mm away from the injection site.In addition,hNSCs were observed 10 weeks after transplantation.At week 4,BBB locomotor scores were significantly greater in the genetically modified hNSC and normal hNSC groups,compared with the model group (P<0.05),and scores were significantly greater in the genetically modified hNSC group compared with the normal hNSC group(P<0.05). CONCLUSION:hNSCs were genetically modified with lentivirus to stably secrete neurotrophin-3. hNSCs improved motor function recovery in rats following spinal cord injury. | Peiqiang Cai Guangyun Sun Peishu Cai Martin Oudega Rui Xiao Xuewen Wang Wei Li Yunbing Shu Cheng Cai Haihao Yang Xuebing Shan Wuhua Luo | 2009 | Neural Regeneration Research2009，4，7： | 18 |
| 11 | Transurethral seminal vesiculoscopy for recurrent hemospermia: experience from 419 cases显示文摘 | Rui Chen Lei Wang Xia Sheng Shu-Guang Piao Xin-Wen Nian Xin Cheng Tie Zhou Hui-Zhen Li Ya-Wei Liu Guang-Hua Chen Chun-Lei Zhang De-Pei Kong Guang-An Xiao Xin Lu Zhen-Yu Jia Zhi-Yong Liu Ying-Hao Sun | 2018 | Asian Journal of Andrology2018，20，5： | 18 |
| 12 | The role of the HIF-1α/ALYREF/PKM2 axis in glycolysis and tumorigenesis of bladder cancer显示文摘Background:As a rate-limiting enzyme of glycolysis,pyruvate kinase muscle isozyme M2(PKM2)participates in tumor metabolism and growth.The regulatory network of PKM2 in cancer is complex and has not been fully studied in bladder cancer.The 5-methylcytidine(m5C)modification in PKM2 mRNA might participate in the pathogenesis of bladder cancer and need to be further clarified.This study aimed to investigate the biological function and regulatory mechanism of PKM2 in bladder cancer.Methods:The expression of PKM2 and Aly/REF export factor(ALYREF)was measured by Western blotting,qRT-PCR,and immunohistochemistry.The bioprocesses of bladder cancer cells were demonstrated by a series of experiments in vitro and in vivo.RNA immunoprecipitation,RNA-sequencing,and dualluciferase reporter assays were conducted to explore the potential regulatory mechanisms of PKM2 in bladder cancer.Results:In bladder cancer,we first demonstrated that ALYREF stabilized PKM2 mRNA and bound to its m5C sites in 3′-untranslated regions.Overexpression of ALYREF promoted bladder cancer cell proliferation by PKM2-mediated glycolysis.Furthermore,high expression of PKM2 and ALYREF predicted poor survival in bladder cancer patients.Finally,we found that hypoxia-inducible factor-1alpha(HIF-1α)indirectly up-regulated the expression of PKM2 by activating ALYREF in addition to activating its transcription directly.Conclusions:The m5C modification in PKM2 mRNA in the HIF-1α/ALYREF/PKM2 axis may promote the glucose metabolism of bladder cancer,providing a new promising therapeutic target for bladder cancer. | Jing-Zi Wang Wei Zhu Jie Han Xiao Yang Rui Zhou Hong-Cheng Lu Hao Yu Wen-Bo Yuan Peng-Chao Li Jun Tao Qiang Lu Ji-Fu Wei Haiwei Yang | 2021 | Cancer Communications2021，41，7： | 17 |
| 13 | Carotid Atherosclerosis and Cognitive Impairment in Nonstroke Patients显示文摘 | Wei-Hong Chen Wei Jin Pei-Yuan Lyu Yang Liu Rui Li Ming Hu Xiang-Jian Xiao | 2017 | Chinese Medical Journal2017，，19： | 15 |
| 14 | Therapeutic approaches to non-alcoholic fatty liver disease: past achievements and future challenges显示文摘BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver injury and mortality in Western countries and China. However, as to date, there is no direct and effective therapy for this disease. The aim of this review is to analyze the key progress and challenges of main current therapeutic approaches in NAFLD. DATA SOURCE: We carried out a PubMed search of English-language articles relevant to NAFLD therapy. RESULTS: There are two major therapeutic strategies for NAFLD treatment: (1) lifestyle interventions (including weight reduction, dietary modification and physical exercise) and (2) pharmaceutical therapies. Lifestyle interventions, particularly chronic and moderate intensity exercise, are the most effective and recognized clinical therapies for NAFLD. For pharmaceutical therapies, although their effects and mechanisms have been extensively investigated in laboratory studies, they still need further tests and investigations in clinical human trials. CONCLUSION: Future advancement of NAFLD therapy should focus on the mechanistic studies on cell based and animal models and human clinical trials of exercise, as well as the combination of lifestyle intervention and pharmaceutical therapy specifically targeting main signaling pathways related to lipid metabolism, oxidative stress and inflammation. | Jia Xiao Rui Guo Man Lung Fung Emily C Liong George L Tipoe | 2013 | Hepatobiliary & Pancreatic Diseases International2013，12，2： | 14 |
| 15 | Molecular cloning and functional analysis of the FLOWERING LOCUS T(FT) homolog Gh FT1 from Gossypium hirsutum显示文摘FLOWERING LOCUS T(FT) encodes a member of the phosphatidylethanolamine-binding protein(PEBP) family that functions as the mobile floral signal, playing an important role in regulating the floral transition in angiosperms. We isolated an FT-homolog(Gh FT1) from Gossypium hirsutum L. cultivar,Xinluzao 33 Gh FT1 was predominantly expressed in stamens and sepals, and had a relatively higher expression level during the initiation stage of fiber development. Gh FT1 m RNA displayed diurnal oscillations in both long-day and short-day condition,suggesting that the expression of this gene may be under the control of the circadian clock. Subcellular analysis revealed that Gh FT1 protein located in the cytoplasm and nucleus. Ectopic expression of Gh FT1 in transgenic arabidopsis plants resulted in early flowering compared with wild-type plants. In addition,ectopic expression of Gh FT1 in arabidopsis ft-10 mutants partially rescued the extremely late flowering phenotype. Finally, several Researchflowering related genes functioning downstream of At FT were highly upregulated in the 35S::Gh FT1 transgenic arabidopsis plants. In summary, Gh FT1 is an FT-homologous gene in cotton that regulates flower transition similar to its orthologs in other plant species and thus it may be a candidate target for promoting early maturation in cotton breeding. | Danli Guo Chao Li Rui Dong Xiaobo Li Xiangwen Xiao Xianzhong Huang | 2015 | Journal of Integrative Plant Biology2015，57，6： | 14 |
| 16 | Novel and potent inhibitors targeting DHODH are broad-spectrum antivirals against RNA viruses including newly-emerged coronavirus SARS-CoV-2显示文摘Emerging and re-emerging RNA viruses occasionally cause epidemics and pandemics worldwide,such as the on-going outbreak of the novel coronavirus SARS-CoV-2.Herein,we identified two potent inhibitors of human DHODH,S312 and S416,with favorable drug-likeness and pharmacokinetic profiles,which all showed broad-spectrum antiviral effects against various RNA viruses,including influenza A virus,Zika virus,Ebola virus,and particularly against SARS-CoV-2.Notably,S416 is reported to be the most potent inhibitor so far with an EC5o of 17 nmol/L and an SI value of 10,505.88 in infec-ted cells.Our results are the first to validate that DHODH is an attractive host target through high antiviral efficacy in vivo and low virus replication in DHODH knock-out cells.This work demonstrates that both S312/S416 and old drugs(Leflunomide/Teriflunomide)with dual actions of antiviral and immuno-regulation may have clinical potentials to cure SARS-CoV-2 or other RNA viruses circulating worldwide,no matter such viruses are mutated or not. | Rui Xiong Leike Zhang Shiliang Li Yuan Sun Minyi Ding Yong Wang Yongliang Zhao Yan Wu Weijuan Shang Xiaming Jiang Jiwei Shan Zihao Shen Yi Tong Liuxin Xu Yu Chen Yingle Liu Gang Zou Dimitri Lavillete Zhenjiang Zhao Rui Wang Lili Zhu Gengfu Xiao Ke Lan Honglin Li Ke Xu | 2020 | Protein & Cell2020，11，10： | 14 |
| 17 | Prolonged manganese exposure induces severe deficits in lifespan,development and reproduction possibly by altering oxidative stress response in Caenorhabditis elegans显示文摘We examined the possible multiple defects induced by acute and prolonged exposure to high levels of manganese(Mn) solution by monitoring the endpoints of lifespan,development,reproduction,and stress response.Our data suggest that acute exposure(6 h) to Mn did not cause severe defects of life span,development,and reproduction,similarly,no significant defect could be found in animals exposed to a low concentration of Mn(2.5 μmol/L) for 48 h.In contrast,prolonged exposure(48 h) to high Mn concentrations(75 and 200 μmol/L) resulted in significant defects of life span,development,and reproduction,as well as the increase of the percentage of population with hsp-16.2::gfp expression indicating the obvious induction of stress responses in exposed animals.Moreover,prolonged exposure(48 h) to high concentrations(75 and 200 μmol/L) of Mn decreased the expression levels of antioxidant genes of sod-1,sod-2,sod-3,and sod-4 compared to control.Therefore,prolonged exposure to high concentrations of Mn will induce the severe defects of life span,development,and reproduction in nematodes possibly by affecting the stress response and expression of antioxidant genes in Caenorhabditis elegans. | XIAO Jing | 2009 | Journal of Environmental Sciences2009，21，6： | 13 |
| 18 | Management of granulomatous lobular mastitis: an international multidisciplinary consensus(2021 edition)显示文摘Granulomatous lobular mastitis(GLM) is a rare and chronic benign inflammatory disease of the breast. Difficulties exist in the management of GLM for many front-line surgeons and medical specialists who care for patients with inflammatory disorders of the breast. This consensus is summarized to establish evidence-based recommendations for the management of GLM. Literature was reviewed using PubMed from January 1, 1971 to July 31, 2020. Sixty-six international experienced multidisciplinary experts from 11 countries or regions were invited to review the evidence.Levels of evidence were determined using the American College of Physicians grading system, and recommendations were discussed until consensus. Experts discussed and concluded 30 recommendations on historical definitions,etiology and predisposing factors, diagnosis criteria, treatment, clinical stages, relapse and recurrence of GLM. GLM was recommended as a widely accepted definition. In addition, this consensus introduced a new clinical stages and management algorithm for GLM to provide individual treatment strategies. In conclusion, diagnosis of GLM depends on a combination of history, clinical manifestations, imaging examinations, laboratory examinations and pathology.The approach to treatment of GLM should be applied according to the different clinical stage of GLM. This evidencebased consensus would be valuable to assist front-line surgeons and medical specialists in the optimal management of GLM. | Qian-Qian Yuan Shu-Yuan Xiao Omar Farouk Yu-Tang Du Fereshte Sheybani Qing Ting Tan Sami Akbulut Kenan Cetin Afsaneh Alikhassi Rami Jalal Yaghan Irmak Durur-Subasi Fatih Altintoprak Tae Ik Eom Fatih Alper Mustafa Hasbahceci David Martínez-Ramos Pelin Seher Oztekin Ava Kwong Cedric W.Pluguez-Turul Kirstyn EBrownson Shirish Chandanwale Mehran Habib Liu-Yi Lan Rui Zhou Xian-Tao Zeng Jiao Bai Jun-Wen Bai Qiong-Rong Chen Xing Chen Xiao-Ming Zha Wen-Jie Dai Zhi-Jun Dai Qin-Yu Feng Qing-Jun Gao Run-Fang Gao Bao-San Han Jin-Xuan Hou Wei Hou Hai-Ying Liao Hong Luo Zheng-Ren Liu Jing-Hua Lu Bin Luo Xiao-Peng Ma Jun Qian Jian-Yong Qin Wei Wei Gang Wei Li-Ying Xu Hui-Chao Xue Hua-Wei Yang Wei-Ge Yang Chao-Jie Zhang Fan Zhang Guan-Xin Zhang Shao-Kun Zhang Shu-Qun Zhang Ye-Qiang Zhang Yue-Peng Zhang Sheng-Chu Zhang Dai-Wei Zhao Xiang-Min Zheng Le-Wei Zheng Gao-Ran Xu Wen-Bo Zhou Gao-Song Wu | 2022 | Military Medical Research2022，9，4： | 13 |
| 19 | DNMT3A/3B overexpression might be correlated with poor patient survival, hypermethylation and low expression of ESR1/PGR in endometrioid carcinoma: an analysis of The Cancer Genome Atlas显示文摘Background:DNA methylation is involved in numerous biologic events and associates with transcriptional gene silencing, playing an important role in the pathogenesis of endometrial cancer.ESR1/PGR frequently undergoes de novo methylation and loss expression in a wide variety of tumors, including breast, colon, lung, and brain tumors.However, the mechanisms underlying estrogen and progesterone receptors (ER/PR) loss in endometrial cancer have not been studied extensively.The aims of this study were to determine the expression of DNA (cytosine-5)-methyltransferase 3A/3B (DNMT3A/3B) in endometrial cancer to investigate whether the methylation catalyzed by DNMT3A/3B contributes to low ER/PR expression.Methods:The clinicopathologic information and RNA-Seq expression data of DNMT3A/3B of 544 endometrial cancers were derived from The Cancer Genome Atlas (TCGA) uterine cancer cohort in May 2018.RNA-Seq level of DNMT3A/3B was compared between these clinicopathologic factors with t-test or one-way analysis of variance.Results:DNMT3A/3B was overexpressed in endometrioid carcinoma (EEC) and was even higher in non-endometrioid carcinoma (NEEC) (DNMT3A, EEC vs.NEEC:37.6% vs.69.9%, t=-7.440, P<0.001;DNMT3B, EEC vs.NEEC:42.4% vs.72.8 %, t=-6.897, P<0.001).In EEC, DNMT3A overexpression was significantly correlated with the hypermethylation and low expression of the ESR1 and PGR (P<0.05).The same trend was observed in the DNMT3B overexpression subgroup.In the ESR1/PGR low-expression subgroups, as much as 83.1% of ESR1 and 59.5% of PGR were hypermethylated, which was significantly greater than the ESR1/PGR high-expression subgroups (31.3% and 11.9%, respectively).However, the above phenomena were absent in NEEC, while DNMT3A/3B overexpression, ESR1/PGR hypermethylation, and low ER/PR expression occurred much more often.In univariate analysis, DNMT3A/3B overexpressions were significantly correlated with worse prognosis.In multivariate analysis, only DNMT3A was an independent predictor of disease-free survival (P<0.05).Conclusions:DNMT3A/3B expression increases progressively from EEC to NEEC and is correlated with poor survival.The mechanisms underlying low ER/PR expression might be distinct in EEC vs.NEEC.In EEC, methylation related to DNMT3A/3B overexpression might play a major role in ER/PR downregulation. | Dan He Xiao Wang Yan Zhang Jian Zhao Rui Han Ying Dong | 2019 | Chinese Medical Journal2019，，2： | 12 |
| 20 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
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