|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Preclinical evaluation of azathioprine plus buthionine sulfoximine in the treatment of human hepatocarcinoma and colon carcinoma显示文摘AIM: To evaluate the efficacy and the safety of azathioprine (AZA) and buthionine sulfoximine (BSO) bylocalized application into HepG2 tumor in vivo.METHODS: Different hepatoma and colon carcinoma cell lines (HepG2, HuH7, Chang liver, LoVo, RKO, SW-48, SW-480) were grown in minimal essencial medium supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained in a humidified 37 ℃ incubator with 5% CO2. These cells were pretreated with BSO for 24 h and then with AZA for different times. We examined the effects of this combination on some proteins and on cellular death. We also studied the eff icacy and the safety of AZA (6 mg/kg per day) and BSO (90 mg/kg per day) in HepG2 tumor growth in vivo using athymic mice. We measured safety by serological markers such as aminotransferases and creatine kinase.RESULTS: The in vitro studies revealed a new mechanism of action for the AZA plus BSO combination in the cancer cells compared with other thiopurines (6-mercaptopurine, 6-methylmercaptopurine, 6-thioguanine and 6-methylthioguanine) in combination with BSO. The cytotoxic effect of AZA plus BSO in HepG2 cells resulted from necroptosis induction in a mitochondrial-dependent manner. From kinetic studies we suggest that glutathione (GSH) depletion stimulates c-Jun amino-terminal kinase and Bax translocation in HepG2 cells with subsequent deregulation of mitochondria (cytochrome c release, loss of membrane potential), and proteolysis activation leading to loss of membrane integrity, release of lactate dehydrogenase and DNA degradation. Some of this biochemical and cellular changes could be reversed by N-acetylcysteine (a GSH replenisher). In vivo studies showed that HepG2 tumor growth was inhibited when AZA was combined with BSO.CONCLUSION: Our studies suggest that a combination of AZA plus BSO could be useful for localizedtreatment of hepatocellular carcinoma as in the currently used transarterial chemoembolization method. | Borja Hernández-Breijo Jorge Monserrat Sara Ramírez-Rubio Eva P Cuevas Diana Vara Inés Díaz-Laviada M Dolores Fernández-Moreno Irene D Román Javier P Gisbert Luis G Guijarro | 2011 | World Journal of Gastroenterology2011，17，34： | 2 |
| 2 | Relation of atrophic gastritis with Helicobacter pylori-CagA^+ and interleukin-1 gene polymorphisms显示文摘AIM: To determine the association of Helicobacter pylori (H pylori) CagA+ infection and pro-inflammatory poly-morphisms of the genes interleukin (IL)-1RN and IL-1B with the risk of gastric atrophy and peptic ulcers in a dyspeptic population in Costa Rica, a country with high incidence and mortality of gastric cancer. METHODS: Seven biopsy specimens, a fasting blood sample and a questionnaire concerning nutritional and sociodemographic factors were obtained from 501 con-secutive patients who had undergone endoscopy for dyspeptic symptoms. A histopathological diagnosis was made. Pepsinogen concentrations were analyzed by enzyme linked immunosorbent assay (ELISA). Infection with H pylori CagA+ was determined by serology and polymerase chain reaction (PCR). IL-1B and IL-1RN polymorphisms genotyping was performed by PCR-restriction fragment length polymorphism (PCR-RFLP)and PCR respectively. RESULTS: In this dyspeptic population, 86% were H pylori positive and of these, 67.8% were positive for CagA. Atrophic antral gastritis (AAG) was associated with CagA+ status [odd ratio (OR) = 4.1; P < 0.000] and fruit consumption (OR = 0.3; P < 0.00). Atrophic body gastritis (ABG) was associated with pepsinogen PGI/PGII < 3.4 (OR = 4.9; P < 0.04) and alcohol consumption (OR = 7.3; P < 0.02). Duodenal ulcer was associated with CagA+ (OR = 2.9; P < 0.04) and smoking (OR = 2.4; P < 0.04). PGI < 60 μg/L as well as PGI/PGII < 3.4 were associated with CagA+. CONCLUSION: In a dyspeptic population in Costa Rica, H pylori CagA+ is not associated with ABG, but it is a risk factor for AAG. The pro-inflammatory cytokine poly-morphisms IL-1B + 3945 and IL-1RN are not associated with the atrophic lesions of this dyspeptic population. | Rafaela Sierra Clas Une Vanessa Ramírez Warner Alpízar-Alpízar María I González José A Ramírez Antoine de Mascarel Patricia Cuenca Guillermo Pérez-Pérez Francis Mégraud | 2008 | World Journal of Gastroenterology2008，14，42： | 2 |
| 3 | Current trends of liver cirrhosis in Mexico: Similitudes and differences with other world regions显示文摘AIM To investigate the main current etiologies of cirrhosis in Mexico.METHODS We performed a cross-sectional retrospective multicenter study that included eight hospitals in different areas of Mexico. These hospitals provide health care to people of diverse social classes. The inclusion criteria were a histological, clinical, biochemical, endoscopic, or imaging diagnosis of liver cirrhosis. Data were obtained during a 5-year period(January 2012-December 2017). RESULTS A total of 1210 patients were included. The mean age was 62.5 years(SD = 12.1), and the percentages of men and women were similar(52.0% vs 48.0%). The most frequent causes of liver cirrhosis were hepatitis C virus(HCV)(36.2%), alcoholic liver disease(ALD)(31.2%), and nonalcoholic steatohepatitis(23.2%), and the least frequent were hepatitis B virus(1.1%), autoimmune disorders(7.3%), and other conditions(1.0%).CONCLUSION HCV and ALD are the most frequent causes of cirrhosis in Mexico. However, we note that non-alcoholic fatty liver disease(NAFLD) as an etiology of cirrhosis increased by 100% compared with the rate noted previously. We conclude that NAFLD will soon become one of the most frequent etiologies of liver cirrhosis in Mexico. | Nahum Méndez-Sánchez Felipe Zamarripa-Dorsey Arturo Panduro Emma Purón-González Edgar Ulises Coronado-Alejandro Carlos Alejandro Cortez-Hernández Fátima Higuera de la Tijera José Luis Pérez-Hernández Eira Cerda-Reyes Heriberto Rodríguez-Hernández Vania César Cruz-Ramón Oscar Lenin Ramírez-Pérez Nancy Edith Aguilar-Olivos Olga Fabiola Rodríguez-Martínez Samantha Cabrera-Palma Guillermo Cabrera-álvarez | 2018 | World Journal of Clinical Cases2018，6，15： | 2 |
| 4 | 显示文摘 | Ram M K Adami M Faraci P | 2000 | Polymer2000，41，： | 1 |
| 5 | Indirect enzyme-linked immunosorbent assay for the mycotoxin zearalenone显示文摘 | Liu M T Ram B P Hart L P and Pestka J J | 1985 | Appl Environ Microbiol1985，50，2： | 1 |
| 6 | Self-aligned graphene field-effect transistors with poly- ethyleneimine doped source/drain access regions 显示文摘 | MOVVA H C P RAM M E CORBET C M | 2012 | Applied Physics Letters2012，101，18： | 1 |
| 7 | RANKL/OPG in primary cultures of osteoblasts from post-menopausal women. Differences between osteoporotic hip fractures and osteoarthritis显示文摘 | Mercè Giner M a José Rios M a José Montoya M a Angeles Vázquez Latifa Naji Ramón Pérez-Cano | 2008 | Journal of Steroid Biochemistry and Molecular Biology2008，，1： | 1 |
| 8 | Dynamic aspects of alcoholic fermentation of rice seedlings in response to anoxia and to complete submergence: Relationship to submergence tolerance 显示文摘 | Boamfa E I Ram P C Jackson M B | 2003 | Annals of Botany2003，91，： | 1 |
| 9 | A comparison of modeling and imaginary in the acqui- sition and retention of motor skills 显示文摘 | Ram N Riggs S M Landers D M McCullagh P | 2007 | Journal of Sports Sciences2007，25，5： | 1 |
| 10 | Influence of dietary HUFA levels on reproductive performance, tissue fatty acid profile and desaturase and elongase mRNAs expression in female zebrafish( Danio rerio) 显示文摘 | Jaya - Ram A Kuah M K Lim P S | 2008 | Aquaculture2008，277，： | 1 |
| 11 | Community proteomics of a natural microbial biofilm 显示文摘 | RAM R J VERBEKOMES N C THELEN M P | 2005 | Science2005，308，5730： | 1 |
| 12 | A model for parallel programming over CORBA 显示文摘 | Ram D J Srinivas A V Rani P M | 2004 | Journal of Parallel and Distributed Computing2004，64，11： | 1 |
| 13 | A molecular and genomic view of the fungal cell wall显示文摘 | Klis F M Ram A F J De Groot P W J | 2007 | Biology of the Fungal Cell2007，8，： | 1 |
| 14 | Optimum conditions for in vitro chicken IL-2 production an castle disease vaccinated chickens显示文摘 | Thiagarajan D Ram G C Bansal M P | 1999 | Vet Immunol Immuno pathol1999，67，1： | 1 |
| 15 | Evaluation of metal contamination in coastal sediments of the Bay of Bengal, India: geochemical and statistical approaches 显示文摘 | SELVARAJ K RAM M V SZEFER P | 2004 | Marine Pollution Bulletin2004，49，3： | 1 |
| 16 | Physical insight in the in-situ self-assembled films of polypyrrole显示文摘 | Ram M K Adami M Faraci P | 2000 | Polymer2000，41，： | 1 |
| 17 | Eosinophil cationic protein ( ECP ) : molecular and biological properties and the use of ECP as a marker of eosinophil activationin disease显示文摘 | Venge P Byst ram J Carlson M | 1999 | Clin Exp Allergy1999，29，9： | 1 |
| 18 | Cost layered double hydroxides for CO2 capture:Structure evolution and regeneration显示文摘 | RAM REDDY M K XU Z P LU G Q DINIZ da COSTA J C | 2006 | Ind Eng Chem Res2006，45，22： | 1 |
| 19 | Serotonin-induced parturition in the fingernail clam (Sphaerium musculium ) transversum (Say) 显示文摘 | Fong P P Wall D M Ram J L | 1995 | J Exp Zool1995，272，： | 1 |
| 20 | Enterococci in river Ganga surface waters: propensity of species distribution, dissemination of antimicrobial-resistance and virulence-markers among species along landscape显示文摘 | Lata P Ram S Agrawal M | 2009 | BMC Microbiology2009，9，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员