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| 1 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 2 | Structural coupling of the Dabie Orogen with Hefei Basin显示文摘Geological, geophysical and geochemical data show that both of the Dabie Orogen and Hefei Basin formed during a Mesozoic orogeny. A three-stage development of the Hefei Basin includes down-warping, half grabben, and positive inversion. Both the uplift in orogen and the depression in basin resulted from the 'de-rooting' of orogen and up-welling of the mantle. | Wang, QC Cong, B Ma, L | 1997 | Chinese Science Bulletin1997，42，18： | 10 |
| 3 | Microstructure of Al-Si Alloys Rapidly Solidified from the Different Temperature Melts显示文摘Hypo- and hyper-eutectic Al-Si alloys were rapidly solidified from the low or high temperature melt using water quenching and melt-spinning technique. The differences in microstructure of rapid solidification alloys were studied by scanning and transmission electron microscopy,and X-ray diffraction methods. The primary silicon in the Al-22(wt pct)Si alloy quenched in room temperature water from high temperature melt was smaller than that quenched from low temperature melt. Compared with the ribbons spun from low temperature melt, the ribbons spun from high temperature melt had finer Al-rich and Si-rich phase particles in either Al-7(wt pct)Si or Al-18(Wt pct)Si alloy. The mechanism was explained by the nature of the liquid | Gui, MC Jia, J Song, GS Li, QC | 1999 | Journal of Materials Science & Technology1999，15，3： | 7 |
| 4 | Microstructure and Mechanical Property of in-situ Al-Cu/TiC Composites显示文摘An approach named direct reaction synthesis (DRS) has been developed to fabricate particulate composites with an extremely fine reinforcement size. ID situ Al matrix composites were fabri-cated by DRS. Extensive analysis of the composites microstructure using SEM and TEM identify that the reinforcement formed during the DRS process is Ti carbide (TiC) particle, generally less than 1.0 μm. The reacted, semisolid extruded samples exhibit a homogeneous distribution of fine TiC particles in Al-Cu matrix, Mechanical property evaluation of the composites has revealed a very high tensile strength relative to the matrix alloy. Fractographic analysis indicates ductile failure although the ductility and strength are limited by the presence of coarse titanium aluminides (Al3Ti). | Zhang, EL Zeng, SY Yang, B Li, QC Ma, MZ | 1998 | Journal of Materials Science & Technology1998，14，3： | 6 |
| 5 | Intermediate-acidic silicate melt found in continental mantle of East China显示文摘CO2-RICH fluid inclusions have been found in 6 anhydrous peridotite xenolith minerals in Ceno-zoic basalts of 5 areas in East China.Electron probe backscattering electron image reveals thatseveral inclusions appear in all the rock sections,completely filled with glass or with cavities attheir center part(CO2 has escaped).Some inclusions show devitrification and daughter miner-als appear.We have carried out the analysis on the non-devitrification glass in the inclusions. | Fan, QC Liu, RX Xu, P Lin, ZR | 1997 | Chinese Science Bulletin1997，42，10： | 6 |
| 6 | 提高喷枪使用寿命显示文摘1前言艾萨法炼铅属于熔池熔炼,是直接炼铅工艺的一种。驰宏公司用艾萨炉处理铅精矿属世界首创,它的成功应用,标志着世界铅冶炼水平又迈上了一个新的台阶。进入2008年后,艾萨炉的处理能力和产品产量明显提升,主要技术经济指标已经达到或超过了设计水平。 | 艾萨炉炼铅工艺QC小组 | 2009 | 云南冶金2009，38，S2： | 3 |
| 7 | 寒区路面(英)显示文摘The article mainly discusses several essential problems of cold region pavement, including thermal cracking of asphalt concrete, cracking deterioration and heaving, frost heave, seasonal and long term roughness induced by different frost heave, frost heave cracking, bearing capacity loss during spring thaw. The reason for these problems is that cold region pavements are subjected to intense solicitation by climatic and environmental factors. The author offers several models corresponding to the solicitation. Furthermore in conclusion of the article the author indicates future research for cold region. | Guy Dore (Civil Engineering Department, Laval University,Quebec (QC) G1K 7P4, Canada) | 2002 | 冰川冻土2002，24，5： | 3 |
| 8 | 运用QC小组活动提高反滤料成品的合格率初探显示文摘南水北调中线工程潮河段三标,在渠道衬砌施工进入最后的攻坚阶段时,反滤料成品的合格率低,成了制约施工进度的瓶颈。项目部通过此次QC活动,把反滤料成品的合格率提高了11个百分点,完成了既定目标,用保质保量的反滤料使得工程按期完成。项目部最后把攻关成果归纳整理,并对活动中采取的对策制订了相应的巩固措施。 | 黄志伟 李栋 武朝辉 南水北调中线工程潮河三标伟才QC小组 | 2014 | 水利建设与管理2014，34，11： | 2 |
| 9 | Taxuchin B,a New Chlorine-Containing Taxoid显示文摘A new taxoid, taxuchin B[1], has been isolated from twigs and needles of Taxus chinensis. It is the first chlorine-containing taxoid reported to | Fang, WS Fang, QC Liang, XT Lu, Y Wu, N Zheng, QT | 1997 | Chinese Chemical Letters1997，8，3： | 2 |
| 10 | 软件性能测试中的性能监控方法显示文摘在软件的性能测试整个流程当中,监控起着至关重要的作用。因为在性能测试开始执行之后,需要实时的去观察性能测试的各个指标是否正常,其中包括应用服务器、数据库、中间件等方面。一旦发现异常情况,及时修正,保证性能测试的顺利进行。而且在监控当中,也可以发现系统的瓶颈,适当制止性能测试的继续运行,保证避免重复的工作。 | qc | 2010 | 网络与信息2010，24，12： | 2 |
| 11 | Lack of the vitamin D re ceptor is associated with reduced epidermal differentiation and hair follicle growth显示文摘 | Xie Z Komuves L Yu QC | 2002 | J Invest Dermatol2002，118，1： | 1 |
| 12 | Role of the C terminus Gag protein in human immunodeficiency virus type 1 virion assembly and maturation显示文摘 | Yu XF Matsuda Z Yu QC | 1995 | J Gen Virol1995，76，： | 1 |
| 13 | Role of hydrogen sulphide in the cardioprotection caused by ischemic preconditioning in the rat heart and cardiac myocytes显示文摘 | Yong QC Pan TY | 2006 | J Pharmacol Exp Ther2006，316，： | 1 |
| 14 | Physiology of the eustachian tube in the pathogenesis and management of middle ear effusion显示文摘 | Bluestone CD Paradise JI Beery QC | 1972 | Laryngoscope1972，82，9： | 1 |
| 15 | Guidelines for the Management of Vasa Previa 显示文摘 | Gagnon R Montreal QC | 2009 | J Obstet Gynaecol Can2009，31，8： | 1 |
| 16 | 显示文摘 | XuF CaiR J Zeng QC | 2011 | J Mater Chem2011，21，6： | 1 |
| 17 | Effects of static magnetic field on bone formation of rat femurs显示文摘 | Yan QC Tomita N Ikada Y | 1998 | Med Eng Phys1998，20，6： | 1 |
| 18 | Antidopaminergic antibodies in a patient with a complex autoimmune disorder and rapidly progressing Parkinson's disease显示文摘 | Kuans QC Mcrae A Kesselring J | 1995 | J Allergy Clin Immunol1995，96，： | 1 |
| 19 | Role of peroxisome proliferator-activated receptor alpha in epidermal development in utero显示文摘 | Schmuth M Sehoonjans K Yu Qc | 2002 | J Invest Dermatol2002，119，6： | 1 |
| 20 | Function of the Eustachian tube related to surgical management of acquired aural cholesteatoma in children显示文摘 | Bluestone CD Cantekin EI Beery QC | 1978 | Laryngoscope1978，88，7： | 1 |
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