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| 1 | Distribution of the P2X2 receptor and chemical coding in ileal enteric neurons of obese male mice(ob/ob)显示文摘AIM:To investigate the colocalization,density and profile of neuronal areas of enteric neurons in the ileum of male obese mice.METHODS:The small intestinal samples of male mice in an obese group(OG)(C57BL/6J ob/ob)and a control group(CG)(+/+)were used.The tissues were analyzed using a double immunostaining technique for immunoreactivity(ir)of the P2X2 receptor,nitric oxide synthase(NOS),choline acetyl transferase(ChAT)and calretinin(Calr).Also,we investigated the density and profile of neuronal areas of the NOS-,ChAT-and Calrir neurons in the myenteric plexus.Myenteric neurons were labeled using an NADH-diaphorase histochemical staining method.RESULTS:The analysis demonstrated that the P2X2receptor was expressed in the cytoplasm and in the nuclear and cytoplasmic membranes only in the CG.Neuronal density values(neuron/cm2)decreased 31%(CG:6579±837;OG:4556±407)and 16.5%(CG:7796±528;OG:6513±610)in the NOS-ir and calretininir neurons in the OG,respectively(P<0.05).Density of ChAT-ir(CG:6200±310;OG:8125±749)neurons significantly increased 31%in the OG(P<0.05).Neuron size studies demonstrated that NOS,ChAT,and Calr-ir neurons did not differ significantly between the CG and OG groups.The examination of NADH-diaphorase-positive myenteric neurons revealed an overall similarity between the OG and CG.CONCLUSION:Obesity may exert its effects by promoting a decrease in P2X2 receptor expression and modifications in the density of the NOS-ir,ChAT-ir and CalR-ir myenteric neurons. | Márcia Sanae Mizuno Amanda Rabello Crisma Primavera Borelli Bárbara Tavares Schfer Mariana Póvoa Silveira Patricia Castelucci | 2014 | World Journal of Gastroenterology2014，20，38： | 3 |
| 2 | Expression of the P2X_2 receptor in different classes of ileum myenteric neurons in the female obese ob/ob mouse显示文摘AIM:To examine whether the ob/ob mouse model of obesity is accompanied by enteric nervous system ab-normalities such as altered motility METHODS:The study examined the distribution of the P2X 2 receptor (P2X 2 R) in myenteric neurons of female ob/ob mice. Specifically, we used immunohistochemistry to analyze the co-expression of the P2X 2 R with neuronal nitric oxide synthase (nNOS), choline acetyltrans-ferase (ChAT), and calretinin (CalR) in neurons of the small intestine myenteric plexus in ob/ob and control female mice In these sections, we used scanning confocal microscopy to analyze the co-localization of these markers as well as the neuronal density (cm 2 ) and area profile (μm2) of P2X 2 R-positive neurons In addition, enteric neurons were labeled using the nicotinamide adenine dinucleotide (NA H) diaphorase method and analyzed with light microscopy as an alternate means by which to analyze neuronal density and areaRESULTS:In the present study, we observed a 29 6% increase in the body weight of the ob/ob animals (OG) compared to the control group (CG) In addition, the average small intestine area was increased by approxi-mately 29 6% in the OG compared to the CG Immu-noreactivity (IR) for the P2X 2 R, nNOS, ChAT and CalR was detectable in the myenteric plexus, as well as in the smooth muscle, in both groups This IR appeared to be mainly cytoplasmic and was also associated with the cell membrane of the myenteric plexus neurons, where it outlined the neuronal cell bodies and their processes P2X 2 R-IR was observed to co-localize 100% with that for nNOS, ChAT and CalR in neurons of both groups In the ob/ob group, however, we observed that the neuronal density (neuron/cm 2 ) of P2X 2 R-IR cells was in-creased by 62% compared to CG, while that of NOS-IR and ChAT-IR neurons was reduced by 49% and 57%, respectively, compared to control mice The neuronal density of CalR-IR neurons was not different between the groups Morphometric studies further demonstrated that the cell body profile area (μm2) of nNOS-IR, ChAT-IR and CalR-IR neurons was increased by 34%, 20% and 55%, respectively, in the OG compared to controls Staining for NA H diaphorase activity is widely used to detect alterations in the enteric nervous system; however, our qualitative examination of NA H-diaphorase positive neurons in the myenteric ganglia revealed an overall similarity between the two groups CONCLUSION:We demonstrate increases in P2X2R expression and alterations in nNOS, ChAT and CalR IR in ileal myenteric neurons of female ob/ob mice compared to wild-type controls. | Márcia Sanae Mizuno Amanda Rabello Crisma Primavera Borelli Patricia Castelucci | 2012 | World Journal of Gastroenterology2012，18，34： | 2 |
| 3 | Socio-economic impacts of shrimp culture 显示文摘 | PRIMAVERA J H HONCULADA J H | 1997 | Aquaculture Research1997，28，10： | 1 |
| 4 | Ifosfamide encephalopathy and nonconvulsive status epilepticus 显示文摘 | Primavera A Audenino D Cocito L | 2002 | Can J Neurol Sci2002，29，2： | 1 |
| 5 | Reproductive quality of male Penaeus mondon 显示文摘 | Gomes L A O Honculada - Primavera J | 1993 | Aquaculture1993，112，： | 1 |
| 6 | Effect of aquaculture on world fish supplies显示文摘 | Naylor R L Goldburg R J Primavera J H | 2000 | Nature2000，405，： | 1 |
| 7 | Diffusion MRI in ischemic stroke compared to pathologically verified infarction显示文摘 | Kelly P J Hedley-Whyte E T Primavera J | 2001 | Neurology2001，56，7： | 1 |
| 8 | Endothelial dysfunction and the expression of endothelial nitric oxide synthase in diabetic neuropathy, vascular disease and food ulcrration显示文摘 | Veves A Akbari CM Primavera J | 1998 | Diabetes1998，47，： | 1 |
| 9 | Sensitive skin: mechanisms and diagnosis显示文摘 | Primavera G Berardesca E | 2005 | Int J Cosmet Sci2005，27，： | 1 |
| 10 | Overcoming the impacts of aquaculture on the coastal zone 显示文摘 | Primavera J H | 2006 | Ocean & Coastal Management2006，49，： | 1 |
| 11 | Monocytes from circulating blood fuse in vitro with purified osteoclasts in primary culture显示文摘 | Zambonin Zallone A Teti A Primavera MV | 1984 | J Cell Sci1984，66，： | 1 |
| 12 | Endothelial dysfunction and the expression of endothelial nitric oxide synthetase in diabetic neuropathy,vascular disease, and foot ulceration显示文摘 | Akbari CM Primavera J | 1998 | Diabetes1998，47，： | 1 |
| 13 | Glutathione transferases and neurodegenerative diseases显示文摘 | Mazzetti AP Fiorile MC Primavera A | 2015 | Neurochem Int2015，82，： | 1 |
| 14 | Effect of voids on the reliability of BGA/CSP solder joints 显示文摘 | Yunus M Primavera A SrihariK | 2003 | Microelectronics reliability2003，43，12： | 1 |
| 15 | A ease of secondary syphilis presenting as optic neuritis 显示文摘 | Bandettini di Poggio M Primavera A Capello E | 2010 | Neurol Sci2010，31，3： | 1 |
| 16 | Endothelial dysfunction and the expression of endothelial nitricoxide synthetase in diabetic neuropathy, vascular disease, and foot ulceration 显示文摘 | Veves A Akbari CM Primavera J | 1998 | Diabetes1998，47，3： | 1 |
| 17 | Choosing tropical portunid species for culture, domestication and stock en- hancement in the Indo-Pacific 显示文摘 | Williams M J Primavera J H | 2001 | Asian Fisheries Science2001，14，2： | 1 |
| 18 | Endothelial dysfunction and the expression of endothelial nitric oxide synthetase in diabetic neuropathy,vascular disease and foot ulceration显示文摘 | Veves A Akbari CM Primavera J | 1998 | Diabetes1998，47，3： | 1 |
| 19 | Effect of aquaculture on world fish supplies 显示文摘 | Naylor R L Goldburg R J Primavera J H | 2000 | Nature2000，405，6790： | 1 |
| 20 | Enhancing Family CompetenceThrough Literacy Activities显示文摘 | Judy Primavera | 2000 | Journal of Prevention&Intervention in the Community2000，20，1： | 1 |
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