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| 1 | Pharmacopoeia of the People's Republic of China(2010 Edition):A Milestone in Development of China's Healthcare显示文摘Compilation of the Pharmacopoeia of the People's Republic of China (known as the Chinese Pharmacopoeia 2010) has been completed by the Ninth Chinese Pharmacopoeia Commission, which has been officially distributed in January 2010 and implemented on October 1st, 2010. | QIAN Zhong-zhi1,DAN Yang2,LIU Yan-ze2,PENG Yong2 1.Chinese Pharmacopoeia Commission,Beijing 100061,China 2.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China | 2010 | Chinese Herbal Medicines2010，2，2： | 55 |
| 2 | Invasive mechanism and control strategy of Ageratina adenophora (Sprengel)显示文摘In order to ascertain the invasive mechanism and control strategy of the invasive Crofton weed, Ageratina adenophora, its ecological adaptability and population differentiation,the formation of single dominant population, displacement of native plants and sustainable management strategies were investigated. The present results helped to clarify and explain such issues as the adaptability post invasion,interaction and competition between inter-and intra-species and community resistance, thereby providing important references to researches on other invasive alien species. | WAN FangHao1, LIU WanXue1, GUO JianYing1, QIANG Sheng2, LI BaoPing2, WANG JinJun3, YANG GuoQing1, NIU HongBang1, GUI FuRong1, HUANG WenKun1, JIANG ZhiLin1 & WANG WenQi3 1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China 2College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China 3Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China | 2010 | Science China(Life Sciences)2010，53，11： | 42 |
| 3 | Effect of Silicon on Leaf Ultrastructure, Chlorophyll Content and Photosynthetic Activity of Barley Under Salt Stress显示文摘Two contrasting cultivars of barley (Horderm vykgare L.): Kepin No. 7 (salt sensitive), and Jian 4 (salt tolerant) were grown in a hydroponics system with 2 NaCl levels: 60 mmol NaCl L-1 and 120 mmol NaCl L-1, and 3 Si levels: 0 mmol Si L-1, 0.5 mmol Si L-1 and 1.0 mmol Si L-1 (as silicic acid). Compared with the plants treated with 60 mmol NaCl L-1 alone, the leaf chlorophyll contents of plants treated with salt and Si increased significantly for salt-sensitive cultivar at tillering stage, but for salt-tolerant cultivar,the addition of Si resulted in an obvious increase in the leaf chlorophyll content of plants exposed to 120 mmol NaCl L-1. However, this Si-enhancement of leaf chlorophyll content was also observed in the salttolerant plants at jointing stage, but not in the salt-sensitive plants. Moreover, leaf chlorophyll content was consistently higher for the salt-tolerant cultivar than for the salt-sensitive cultivar irrespective of salt and/or Si treatment. Compared with the plants treated with salt alone, net CO2 assimilation rate in plant leaves increased significantly for both cultivars when trested with salt and Si. The addition of Si to the salt treatment was found to improve the cell ultrastructure of leaves. Under salt stress condition, the double membranes of chloroplasts disappeared, but membrane integrity was markedly improved in the salt treatment supplemented with Si. Silicon was also found to ameliorate the damage to the ultrastructure of chloroplast granae which appeared to be disintegrated and vague in salt treatments without added Si. The results support previous work which showed that Si decreases the permeability of plasma membranes of salt-stressed barley, thus mitigating salt damage. | LIANG YONGCHAO (Department of Plant Nutrition, College of Natural Reaources and Environmental Science, and Ministry of Agriculture Key Laboratory of Crop Growth and Regulation, Nanjing Agricultural University, Nanjing 210095 China) | 1998 | Pedosphere1998，8，4： | 43 |
| 4 | Ideotype root architecture for efficient nitrogen acquisition by maize in intensive cropping systems显示文摘The use of nitrogen(N) fertilizers has contributed to the production of a food supply sufficient for both animals and humans despite some negative environmental impact.Sustaining food production by increasing N use efficiency in intensive cropping systems has become a major concern for scientists,environmental groups,and agricultural policymakers worldwide.In high-yielding maize systems the major method of N loss is nitrate leaching.In this review paper,the characteristic of nitrate movement in the soil,N uptake by maize as well as the regulation of root growth by soil N availability are discussed.We suggest that an ideotype root architecture for efficient N acquisition in maize should include(i) deeper roots with high activity that are able to uptake nitrate before it moves downward into deep soil;(ii) vigorous lateral root growth under high N input conditions so as to increase spatial N availability in the soil;and(iii) strong response of lateral root growth to localized nitrogen supply so as to utilize unevenly distributed nitrate especially under limited N conditions. | MI GuoHua,CHEN FanJun,WU QiuPing,LAI NingWei,YUAN LiXing & ZHANG FuSuo* Key Laboratory of Plant Nutrition,MOA Key Laboratory of Plant-Soil Interaction,MOE College of Resources and Environmental Science,China Agricultural University,Beijing 100193,China | 2010 | Science China(Life Sciences)2010，53，12： | 35 |
| 5 | Characterizations and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping显示文摘Previous study indicated that the thermo-sensitive genic malesterile(TGMS) gene in rice was regulated by temperature.TGMS rice plays an important role in hybrid rice production,because the application of the TGMS system in two-line breeding is laborsaving,timesaving,simple,inexpensive,efficient,and eliminating the limitations of the cytoplasmic male sterility(CMS) system.'AnnongS' is the first discovered and deeply studied TGMS rice lines in China.'AnnongS-1' and 'Y58S',two derivatives of TGMS line AnnongS,were both controlled by a single recessive gene named tms5,which was genetically mapped on chromosome 2.In this study,three populations('AnnongS-1' × 'Nanjing11','Y58S' × 'Q611',and 'Y58S' × 'Guanghui122') were developed and used for the molecular fine mapping of the tms5 gene.By analyzing recombination events in the sterile individuals using a total of 125 probes covering the tms5 region,the tms5 gene was physically mapped to a 19-kb DNA fragment between two markers 4039-1 and 4039-2,which were located on the BAC clone AP004039.After the construction of the physical map between two markers 4039-1 and 4039-2,a member(ONAC023) of the NAC(NAM-ATAF-CUC-related) gene family was identified as the candidate gene of the tms5 gene. | YANG Qing-kai1,LIANG Chun-yang1,LI Jun1,JIN De-min1,AHUANG Wen2,DENG Qi-yun2,WANG Bin1(1.The State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,100101 2. Hunan Hybrid Rice Research Center,Changsha 410125) | 2007 | 湖南农业大学学报(自然科学版)2007，33，S1： | 34 |
| 6 | Assessment of candidate plant DNA barcodes using the Rutaceae family显示文摘DNA barcoding is a rapidly developing frontier technology that is gaining worldwide attention.Here,seven regions (psbA-trnH,matK,ycf5,rpoC1,rbcL,ITS2,and ITS) with potential for use as DNA barcodes were tested for their ability to identify 300 samples of 192 species from 72 genera of the family Rutaceae.To evaluate each barcode’s utility for species authentication,PCR amplification efficiency,genetic divergence,and barcoding gaps were assessed.We found that the ITS2 region exhibited the highest inter-specific divergence,and that this was significantly higher than the intra-specific variation in the 'DNA barcoding gap' assessment and Wilcoxon two-sample tests.The ITS2 locus had the highest identification efficiency among all tested regions.In a previous study,we found that ITS2 was able to discriminate a wide range of plant taxa,and here we confirmed that ITS2 was also able to discriminate a number of closely related species.Therefore,we propose that ITS2 is a promising candidate barcode for plant species identification. | LUO Kun1,2,CHEN ShiLin1,CHEN KeLi2,SONG JingYuan1,YAO Hui1,MA XinYe1,ZHU YingJie3,PANG XiaoHui1,YU Hua1,LI XiWen1,4 & LIU Zhen2 1Institute of Medicinal Plant Development,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100193,China 2College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430061,China 3School of Bioscience and Engineering,Southwest Jiaotong University,Chengdu 610031,China 4Department of Chemistry,Tsinghua University,Beijing 100084,China | 2010 | Science China(Life Sciences)2010，53，6： | 32 |
| 7 | Challenges in Research and Development of Traditional Chinese Medicines显示文摘This review is briefly to recall the history of research and development (R&D) of Chinese materia medica (CMM) and to discuss the challenges of Chinese traditional and herbal medicines (CTHM) facing the modern science and technology. The R&D of CTHM is thought to be an important pathway for new drug discovery. Since1949, about 140 approved new drugs have been developed, among which about 80 originated directly or indirectly from medicinal plants. CTHM has gained interest from the international medical, biomedical, and pharmaceutical institutions as a valuable source of potential medicines. For the modernization of CMM and innovative research of CTHM, there are following challenges to be faced: (1) to evaluate the efficacy, pharmacological properties, action mechanism, and active chemical constituents; (2) to develop new methodologies for the quality and safety of CTHM; (3) to apply new '-omics' techniques to accelerate drug discoveries developed from CTHM; and (4) to apply international practices including good agricultural practice, good manufacturing practice, good laboratory practice, and good clinical practice in the R&D of CTHM. | LIU Chang-xiao1*, XIAO Pei-gen2, PENG Yong2, SONG Nai-ning1 1. Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China | 2009 | Chinese Herbal Medicines2009，1，1： | 34 |
| 8 | Invasive mechanism and management strategy of Bemisia tabaci(Gennadius) biotype B:Progress report of 973 Program on invasive alien species in China显示文摘Bemisia tabaci(Gennadius) biotype B,called a 'superbug',is one of the most harmful biotypes of this species complex worldwide.In this report,the invasive mechanism and management of B.tabaci biotype B,based on our 5-year studies,are presented.Six B.tabaci biotypes,B,Q,ZHJ1,ZHJ2,ZHJ3 and FJ1,have been identified in China.Biotype B dominates the other biotypes in many regions of the country.Genetic diversity in biotype B might be induced by host plant,geographical conditions,and/or insecticidal application.The activities of CarE(carboxylesterase) and GSTs(glutathione-S-transferase) in biotype B reared on cucumber and squash were greater than on other host plants,which might have increased its resistance to insecticides.The higher activities of detoxification enzymes in biotype B might be induced by the secondary metabolites in host plants.Higher adaptive ability of biotype B adults to adverse conditions might be linked to the expression of heat shock protein genes.The indigenous B.tabaci biotypes were displaced by the biotype B within 225 d.The asymmetric mating interactions and mutualism between biotype B and begomoviruses via its host plants speed up widespread invasion and displacement of other biotypes.B.tabaci biotype B displaced Trialeurodes vaporariorum(Westwood) after 4-7 generations under glasshouse conditions.Greater adaptive ability of the biotype B to adverse conditions and its rapid population increase might be the reasons of its successful displacement of T.vaporariorum.Greater ability of the biotype B to switch to different host plants may enrich its host plants,which might enable it to better compete with T.vaporariorum.Native predatory natural enemies possess greater ability to suppress B.tabaci under field conditions.The kairomones in the 3rd and 4th instars of biotype B may provide an important stimulus in host searching and location by its parasitoids.The present results provide useful information in explaining the mechanisms of genetic diversity,evolution and molecular eco-adaptation of biotype B.Furthermore,it provides a base for sustainable management of B.tabaci using biological and ecological measures. | WAN FangHao1,ZHANG GuiFen1,LIU ShuSheng2,LUO Chen3,CHU Dong4,ZHANG YouJun4,ZANG LianSheng2,JIU Min2,Lü ZhiChuang1,CUI XuHong1,ZHANG LiPing4,ZHANG Fan3,ZHANG QingWen5,LIU WanXue1,LIANG Pei5,LEI ZhongRen1 & ZHANG YongJun1 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Acadeny of Agriculture Sciences,Beijing 100094,China 2 Institute of Applied Entomology,Zhejiang University,Hangzhou 310029,China 3 Institute of Plant and Environment Protection,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100089,China 4 Institute of Vegetables and Flowers,Chinese Acadeny of Agricullare Sciences,Beijing 100081,China 5 Department of Entomology,China Agriculture University,Beijing 100094,China | 2009 | Science China(Life Sciences)2009，52，1： | 30 |
| 9 | Identification and mapping of quantitative trait loci controlling cold-tolerance of Chinese common wild rice (O. rufipogon Griff.) at booting to flowering stages显示文摘An advanced backcross population of rice was used to identify the quantitative trait locus (QTL) controlling the cold-tolerance at booting to flowering stages. The recipient, Guichao 2 (GC2), was a commercial Indica rice; the donor Dongxiang common wild rice, was an accession of common wild rice (DXCWR, Oryza rufipogon Griff.). Three QTLs for cold-tolerance were detected on chromosomes 1, 6 and 11. Two of them coming from DXCWR could enhance the cold-tolerance of the backcross progenies. Moreover, one sterility QTL that could reduce the seed set rate of the backcross progenies by 78% was mapped on chromosome 5. | LIU Fengxia, SUN Chuanqing, TAN Lubin, FU Yongcai, LI Dejun & WANG Xiangkun Department of Plant Genetics and Breeding, China Agricultural University, National Key Laboratory of Agrobiology, Key Laboratory of Crop Genetic Improvement and Genome of the Ministry of Agriculture, Beijing 100094, China | 2003 | Chinese Science Bulletin2003，48，19： | 29 |
| 10 | Prevention and Treatment of Osteoporosis with Chinese Herbal Medicines显示文摘In this paper,laboratory reports,medication rules,formulating principles,and research methods are summarized and analyzed,including single herb,compound herbs,and the problems in the prevention and treatment of osteoporosis(OP) within the past decade.With widely recognized efficacy,satisfying achievements have been achieved in preventing and treating OP with Chinese herbal medicines(CHM).However,due to the complex constituents,the pharmacological activities and mechanism of CHM are not clear yet,and there is no unified standard on the diagnosis and syndrome differentiation of OP and the efficacy evaluation of CHM in the treatment.Accordingly,the research in the future should focus on the pharmacology and standardization of CHM in treating OP. | ZHAO Xiao-ling1,2,FENG Yu-xiong1,2,3,PENG Yong1,2 1.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China 2.Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicines,Ministry of Education,Beijing 100193,China 3.College of Basic Medicine,Guangxi University of Chinese Medicine,Nanning 530001,China | 2012 | Chinese Herbal Medicines2012，4，4： | 28 |
| 11 | Identification of quantitative trait loci associated with salt tolerance at seedling stage from Oryza rufipogon显示文摘土壤咸度是影响工厂生长和庄稼生产的主要不能生活的压力之一。在现在的学习,在米饭幼苗舞台的盐忍耐用 87 根基因渗入线(IL ) 被评估,它从在精英 indica 栽培变种 Teqing 和普通野米饭(Oryza rufipogon Griff ) 的就职之间的一个十字被导出。实质的变化为包括盐忍耐 20 的四个特点(圣) 被观察,相对的根干燥重量(RRW ) ,干燥重量(RSW ) 和相对总数弄干的相对射击重量(RTW ) 。圣断然显著地所有另外的三个特点地被相关。与这四个特点联系的 15 通常认为的量的特点 loci (QTL ) 的一个总数用单个点的分析被检测,它位于染色体 1, 2, 3, 6, 7, 9 和 10 与解释 phenotypic 变化的 8%26% 。O。在 13 QTL (86.7%) 的导出 rufipogon 的等位基因能在 Teqing 背景改进盐忍耐。影响 RRW, RSW 和 RTW 的四 QTL 簇在染色体上被发现 6, 7, 9 和 10 分别地。在这四 QTL 簇之中,包括三 QTL (qRRW10, qRSW10 和 qRTW10 ) 的主要的簇在染色体 10 的长手臂上在制造者 RM271 附近被发现,并且 O。分别地,当 phenotypic 变化由这解释为三个特点的三单个 QTL 从 19% ~ 26% 变化了时,在这三 loci 的导出 rufipogon 的等位基因与 22.7% , 17.3% 和 18.5% 的添加剂效果增加了 RRW, RSW 和 RTW。另外,几腌容忍的 IL 被选择并且能被用于识别并且利用有利的盐从普通野米饭的容忍的基因并且在盐使用容忍的米饭繁殖编程序。 | Lei Tian,Lubin Tan,Fengxia Liu,Hongwei Cai,Chuanqing Sun State Key Laboratory of Plant Physiology and Biochemistry,National Centre for Evaluation of Agricultural Wild Plant(Rice), Laboratory of Crop Heterosis and Utilization of Ministry of Education Beijing Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture,Department of Plant Genetics and Breeding,China Agricultural University,Beijing 100193,China | 2011 | Journal of Genetics and Genomics2011，38，12： | 26 |
| 12 | AtHsfA2 modulates expression of stress responsive genes and enhances tolerance to heat and oxidative stress in Arabidopsis显示文摘There is increasing evidence for considerable interlinking between the responses to heat stress and oxidative stress, and recent researches suggest heat shock transcription factors (Hsfs) play an important role in linking heat shock with oxidative stress signals. In this paper, we present evidence that AtHsfA2 modulated expression of stress responsive genes and enhanced tolerance to heat and oxidative stress in Arabidopsis. Using Northern blot and quantitative RT-PCR analysis, we demonstrated that the expression of AtHsfA2 was induced by not only HS but also oxidative stress. By functional analysis of AtHsfA2 knockout mutants and AtHsfA2 overexpressing transgenic plants, we also demonstrated that the mutants displayed reduced the basal and acquired thermotolerance as well as oxidative stress tolerance but the overexpression lines displayed increased tolerance to these stress. The phenotypes correlated with the expres-sion of some Hsps and APX1, ion leakage, H2O2 level and degree of oxidative injuries. These results showed that, by modulated expression of stress responsive genes, AtHsfA2 enhanced tolerance to heat and oxidative stress in Arabidopsis. So we suggest that AtHsfA2 plays an im-portant role in linking heat shock with oxidative stress signals. | LI Chunguang1,2, CHEN Qijun1, GAO Xinqi1,3, QI Bishu1, CHEN Naizhi1, XU Shouming1, CHEN Jia1 & WANG Xuechen1 1. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China 2. Department of Industry Engineering, Zhengzhou Institute of Aeronautic Industry Management, Zhengzhou 450015, China 3. College of Life Science, Qufu Normal University, Qufu Shandong 273165, China | 2005 | Science China(Life Sciences)2005，48，6： | 23 |
| 13 | Relationship between DNA Barcoding and Chemical Classification of Salvia Medicinal Herbs显示文摘Objective To make the identification of medicinal herbs in Salvia L.quickly and accurately.Methods In this work,DNA barcoding and chemical fingerprint were compared for the identification of herbs in Salvia L.First,the nucleotide sequences of the internal transcribed spacer region two amplified from 48 medicinal plants in Salvia L.,and three other groups of medicinal plants in Lamiaceae were sequenced.A molecular phylogeny was constructed using the minimum evolution and maximum parsimony methods according to their sequence diversity.Second,the water-solution bioactive components and lipid soluble components were tested by HPLC.Then a chemical phylogeny was built using HPLC fingerprint data.Comparing the molecular and chemical phylogenetic trees revealed many similarities.Results DNA barcoding was sequencing based and could therefore provide more accurate results within a shorter time especially in large-scale studies.Conclusion The results show that ITS2 region is a novel DNA barcode for the authentication of the species in Salvia L.This is the first work to show the relationship between DNA barcoding and chemical components. | HAN Jian-ping1,LIU Chang2,LI Min-hui1,3,SHI Lin-chun1,SONG Jing-yuan1,YAO Hui1,PANG Xiao-hui1,CHEN Shi-lin1,4 1.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China 2.Molecular Chinese Medicine Laboratory,LKS Faculty of Medicine,The University of Hong Kong,Hong Kong,China 3.Baotou Medical College,Baotou 014040,China 4.Hubei University of Chinese Medicine,Wuhan 430065,China | 2010 | Chinese Herbal Medicines2010，2，1： | 23 |
| 14 | A 60-year journey of mycorrhizal research in China:Past,present and future directions显示文摘The significance of mycorrhizas(fungal roots in 90% of land plants) in plant nutrient acquisition and growth,element biogeo-chemical cycling and maintaining of terrestrial ecosystem structures has been globally established for more than 120 years.Great progress in mycorrhizal research in the past 60 years(1950-2009,1981-2009 in particular) has also been made across China,particularly in the mainland,Hong Kong and Taiwan.For instance,a total of 20 new and ~120 records of arbuscular mycorrhizal(AM) fungal species,30 new and ~800 records of ectomycorrhizal(EM) fungal species,a dozen of new and ~100 records of orchid mycorrhizal(OM) fungal species have been isolated by morphological observation and/or molecular identification in China since the 1950s.Great accomplishment has also been made in the following area,including fungal species richness and genetic structure,relationships between species composition and plant taxa,effects of mycorrhizal fungi on plant nutrient uptake and growth,resistances to pathogens and interactions with other soil microorganisms,potential of mycorrhizal fungi in phytoremediation and/or land reclamation,alterations of enzymatic activities in mycorrhizal plants,and elevated CO2 and O3 on root colonization and species diversity.Unfortunately,the international community cannot easily appreciate almost all Chinese mycorrhizal studies since the vast majority of them have been published in Chinese and/or in China-based journals.The aim of this review is to make a comprehensive exposure of the past and present China's major mycorrhizal research to the whole world,and then to suggest potential directions for the enhancement of future mycorrhizal research within and/or between the Chinese and international mycorrhizal community. | HE XinHua1,2,3*,DUAN YingHua2,CHEN YingLong4 & XU MingGang2 1School of Life Sciences,Yunnan Normal University,Kunming 650092,China 2Key Laboratory of Crop Nutrition and Fertilization,Ministry of Agriculture of China,Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China 3Centre for Ecosystem Management,School of Natural Resources,Edith Cowan University,Joondalup,WA 6027 State Centre of Excellence for Ecohydrology,School of Plant Biology,University of Western Australia,Crawley,WA 6009,Australia 4School of Earth and Environment,University of Western Australia,Crawley,WA 6009,Australia | 2010 | Science China(Life Sciences)2010，53，12： | 23 |
| 15 | Removal of heavy metals from sewage sludge by low costing chemical method and recycling in agriculture显示文摘RemovalofheavymetalsfromsewagesludgebylowcostingchemicalmethodandrecyclinginagricultureWuQitang,NyirandegePascasie,MoCehuiF... | Wu Qi tang, Nyirandege Pascasie, Mo Ce hui Faculty of Resources and Environments, South China Agricultural University, Guangzhou 510642, China Lin Yi Datansha Wastewater Treatment Plant, Guangzhou 510160, China | 1998 | Journal of Environmental Sciences1998，10，1： | 21 |
| 16 | A Genome-wide Functional Characterization of Arabidopsis Regulatory Calcium Sensors in Pollen Tubes显示文摘Calcium, an ubiquitous second messenger, plays an essential and versatile role in cellular signaling. The diverse function of calcium signals is achieved by an excess of calcium sensors. Plants possess large numbers of calcium sensors, most of which have not been functionally characterized. To identify physiologically relevant calcium sensors in a specific cell type, we conducted a genome-wide functional survey in pollen tubes, for which spatiotemporal calcium signals are well-characterized and required for polarized tip growth. Pollen-specific members of calmodulin (CaM), CaM-like (CML), calcium-dependent protein kinase (CDPK) and calcineurin B-like protein (CBL) families were tagged with green fluorescence protein (GFP) and their localization patterns and overexpression phenotypes were characterized in tobacco pollen tubes. We found that several fusion proteins showed distinct overexpression phenotypes and subcellular localization patterns. CDPK24-GFP was localized to the vegetative nucleus and the generative cell/sperms. CDPK32-GFP caused severe growth depolarization. CBL2-GFP and CBL3-GFP exhibited dynamic patterns of subcellular localization, including several endomembrane compartments, the apical plasma membrane (PM), and cytoskeleton-like structures in pollen tubes. Their overexpression also inhibited pollen tube elongation and induced growth depolarization. These putative calcium sensors are excellent candidates for the calcium sensors responsible for the regulation of calcium homeostasis and calciumdependent tip growth and growth oscillation in pollen tubes. | Liming Zhou1,2, Ying Fu1,2 and Zhenbiao Yang2,3 (1State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100193, China 2China Agricultural University (CAU)-University of California, Riverside (UCR) Joint Center for Biological Sciences and Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China 3Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA) | 2009 | Journal of Integrative Plant Biology2009，51，8： | 21 |
| 17 | Measures against the adverse impact of natural wind on air-cooled condensers in power plant显示文摘The natural wind plays disadvantageous roles in the operation of air-cooled steam condensers in power plant.It is of use to take various measures against the adverse effect of wind for the performance improvement of air-cooled condensers.Based on representative 2×600 MW direct air-cooled power plant,three ways that can arrange and optimize the flow field of cooling air thus enhance the heat transfer of air-cooled condensers were proposed.The physical and mathematical models of air-cooled condensers with various flow leading measures were presented and the flow and temperature fields of cooling air were obtained by CFD simulation.The back pressures of turbine were calculated for different measures on the basis of the heat transfer model of air-cooled condensers.The results show that the performance of air-cooled condensers is improved thus the back pressure of turbine is lowered to some extent by taking measures against the adverse impact of natural wind. | YANG LiJun,DU XiaoZe & YANG YongPing Key Laboratory of Condition Monitoring and Control for Power Plant Equipment of Ministry of Education of China,North China Electric Power University,Beijing 102206,China | 2010 | Science China(Technological Sciences)2010，53，5： | 21 |
| 18 | Profile Characteristics and Potential EnvironmentalEffect of Accumulated Phosphorus in Soils of Vegetable Fields in Beijing显示文摘Profile characteristics of accumulated P in 10 representative soils of vegetable fields in suburban districtsof Beijing were investigated. Bioavailability of the accumuIated P and its potential effect on the environmentwere studied in a greenhouse pot experiment and a soil column experiment. The results showed that theconcentration of Olsen-P in the 0-20 cm soil samples of the vegetable fields ranged from 22.1 to 358.0 mgkg-1, which was 2 to 10 times higher than that of the crop fields in the suburbs of Beijing. Most of theexcessive phosphorus was accumulated in the topsoils. The longer the soils cultivated with vegetables, thehigher the soil total P, Olsen-P and organic matter concentration. Accumulated P in the soils from vegetablefields had higher bioavailability. Application of phosphorus fertilizer could not increase the dry weights ofcucumbers, Chinese cabbage, and rape seedlings continuously planted. The soil column study showed highP concentration (> 0.6 mg L-1) in the | SU DECHUN, YANG FENHE and ZHANG FUSUODepartment of Plant Nutrition, China Agricultural University, Beijing 100094 (China) | 2002 | Pedosphere2002，12，2： | 20 |
| 19 | Overexpression of a tobacco small G protein gene NtRop1 causes salt sensitivity and hydrogen peroxide production in transgenic plants显示文摘The small GTPases of Rop/Rho family is central regulators of important cellular processes in plants. Tobacco small G protein gene NtRop1 has been isolated; however, its roles in stress responses were unknown. In the present study, the genomic sequence of NtRop1 was cloned, which has seven exons and six introns, similar to the Rop gene structure from Arabidopsis. The NtRop1 gene was constitutively expressed in the different organs whereas the other six Rop genes from tobacco had differential expression patterns. The expression of the NtRop1 gene was moderately induced by methyl viologen, NaCl, and ACC treatments, but slightly inhibited by ABA treatment, with no significant induction by NAA treatment. The transgenic Arabidopsis plants overexpressing the NtRop1 showed increased salt sensitivity as can be seen from the reduced root growth and elevated relative electrolyte leakage. The hydrogen peroxide production was also promoted in the NtRop1-trangenic plants in comparison with wild type plants. These results imply that the NtRop1 may confer salt sensitivity through activation of H2O2 production during plant response to salt stress. | CAO YangRong1,2, LI ZhiGang1,2, CHEN Tao1,2, ZHANG ZhiGang1, ZHANG JinSong1 & CHEN ShouYi1 1 National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 2 Graduate School of Chinese Academy of Sciences, Beijing 100039, China | 2008 | Science China(Life Sciences)2008，51，5： | 18 |
| 20 | New Collection of Crude Drugs in Chinese Pharmacopoeia 2010 Ⅰ.Callicarpa Linn. and Related Items显示文摘Callicarpa Linn. (beautyberry) is one of the major genera in Verbenaceae, about 20 of which are medicinal plants. Beautyberry, called Zizhu in China, is a generic name of those species and largely used as hemostatic medicine. The Chinese Pharmacopoeia 2010 has admitted three new crude drugs from the genus of Callicarpa Linn. including Callicarpae Macrophyllae Folium, Callicarpae Caulis et Folium, and Callicarpae Formosanae Folium for the first time since the 1977 version of the Chinese Pharmacopoeia. In order to better understand these new crude drugs, we systematically described their bibliography, admission reasons, botanical identification, chemistry, and pharmacology. Several other species, out of national regulations but intensively studied and widely used, are also covered in this review. | DAN Yang1, QIAN Zhong-zhi2, LIU Yan-ze1, ZHOU Guo-ping3, PENG Yong1, XIAO Pei-gen1 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Beijing 100193, China 2. China Pharmacopoeia Committee, Beijing 100061, China 3. Jiangxi Provincial Institute for Food and Drug Control, Nanchang 330046, China | 2010 | Chinese Herbal Medicines2010，2，4： | 18 |
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