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| 1 | Quantitative analysis of vascular endothelial growth factor, microvascular density and their clinicopathologic features in human hepatocellular carcinoma显示文摘BACKGROUND: Angiogenesis is known to be essential to the survival, growth, invasion, and metastasis of tumor cells. Vascular endothelial growth factor (VEGF) are an important angiogenic factor regulating tumor angiogenesis, but its significance and tumor pathologic features are un- clear in hepatocellular carcinoma (HCC). In the present study, we analyzed expression of tissue VEGF, alteration of microvascular density (MVD) in microvessel angiogenesis, development and metastasis of HCC, and level of serum VEGF in differential diagnosis of benign and malignant liv- er diseases. METHODS: Tumor specimens were prospectively collected from HCC patients undergoing resection. Total RNAs were extracted and the expression levels were detected from different parts of HCC tissues. The cellular distributions of VEGF and MVD of liver tumors and their paracancerous and distal cancerous tissues were investigated by streptavi- din peroxidase (S-P) immunohistochemistry, respectively. The VEGF levels of circulating blood and hepatoma tissues were measured by enzyme-linked immunosorbent assay. RESULTS: The incidence of VEGF expression was 63.9% in HCCs (23/36 cases), 78.3% in non-encapsulated HCCs (18/23), and 90.9% in HCCs with extrahepatic metastasis (10/11), respectively. The VEGF expression was tightly correlated with MVD (P <0.01). The MVD in HCC with metastasis, low differentiation or non-encapsulation was significantly higher than that in HCC with intact capsule, high differentiation, or no metastasis. No significant diffe- rence was found between VEGF, MVD, tumor size, and hepatitis virus infection. The level of total RNA in HCC tis- sues was significantly lower but the VEGF level significantly higher than those in paracancerous or distal cancerous ones (P<0.01). The abnormal expression levels of VEGF in sera of HCC patients were directly correlated with the me- tastasis and recurrence of tumors. CONCLUSION: The high expression of VEGF and abnor- mality of tissue MVD are useful predictors for vascular inva- sion and metastasis of liver tumors. | Deng-Fu Yao, Xin-Hua Wu, Yong Zhu, Gong-Sheng Shi, Zhi-Zhen Dong, Deng-Bing Yao, Wei Wu, Li-Wei Qiu and Xian-Yong Meng Nantong, China Research Center of Clinical Molecular Biology , Department of Pathology and Department of Gastroenterology , Affiliated Hos- pital of Nantong University Department of Diagnostics , and Institute of Neurosciences , Nantong University Nantong 226001, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，2： | 81 |
| 2 | Changed clinical aspects of primary liver cancer in China during the past 30 years显示文摘BACKGROUND: Primary liver cancer (PLC) is one of the most frequently seen tumors in China. Thirty years ago, patients with PLC were often detected at relatively late stage, with a palpable mass or marked clinical symptoms and poor prognosis. In the past 30 years, the diagnosis and treatment of PLC have been greatly improved with better prognosis. METHODS: In order to study the changes of PLC during the 30 years, the clinical data of 3250 patients with PLC from 10 medical institutions of China were collected, ana- lyzed , and compared with those of 3254 PLC patients be- fore the 30 years. RESULTS: In the 3250 patients aged 1-80 years, with an average age of 49.1 years, the male to female ratio (2.3:1) was lower than that before the 30 years. 73.5% of the 3250 patients sought medical advice within 3 months after the onset of the disease in contrast to 63.8% before the 30 years. Compared with those patients before the 30 years the symptoms and signs were alleviated generally. The HBsAg positive rate was 81.0%, but the HCV-Ab positive rate was 13.2%. The AFP level in 75% of patients was elevated, but in the remaining 25% was normal. 1912 patients (58.8%) were confirmed pathologically. Among them 1755 patients (91.8%) had hepatocellular carcinoma. The overall resec- tion rate was 46.3%. Those who had early, middle, late stage carcinoma accounted for 29.9%, 51.5%, and 18.6%respectively in contrast to 0.4%, 47.0%, and 52.6% repor- ted before the 30 years. The 1-, 3-, 5-year survival rates of the patients were 66.1%, 39.7%, and 32.5% respectively, whereas 93.5%, 70.1%, and 59.1% for the early stage pa- tients, and 65.3%, 30.5%, and 23.5% for the middle stage patients. The half and 1-year survival rates of the late stage patients were 52.5%, and 14.7%, respectively. CONCLUSION: Comparison with the clinical data before and after the 30 years show that PLC can be diagnosed ear- ly. More PLC patients tend to undergo resection while re- ceiving a better conservative treatment, which ensures a prognosis. | Bing-Hui Yang, Jing-Lin Xia, Li-Wen Huang, Zhao-You Tang, Ming-Shan Chen, Jin-Qing Li, An-Min Liang, Qin-Guo Mo, Hui-Shan Lu, Chao-Liu Dai, Lu-Nan Yan, Zhi-Jian Yu, Rong-Sheng Rao, Le-Qun Li, Zhi-Xiong Su, Zhuang-Wei Fang Shanghai, China Liver Cancer Institute & Zhongshan Hospital, Fudan University, Shanghai 200032, China Tumor Hospital, Zhongshan University, Guangzhou 510000, China Tumor Hospital, Guangxi Medical Universi- ty, Nanning 530000, China Xiehe Hospital, Fujian Medical University, Fuzhou 350000, China Second Hos- pital, Chinese Medical University, Shenyang 110000, China Huaxi Hospital, Sichuan University, Chengdu 610041, China Hospital Affiliated to Nantong Medical University, Nantong 226000 , China Second Hospital, Jiangxi Medical University, Nanchang 330000 , China First Hospital Affiliated to Guangxi Medical University, Nanning 530000 , China People’s Hospital of Hainan Province, Haikou 570000, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，2： | 20 |
| 3 | Bacteriological and electron microscopic examination of primary intrahepatic stones显示文摘BACKGROUND: Primary intrahepatic cholelithiasis is usually combined with biliary tract infection. This research was undertaken to investigate the relationship between intrahepatic stones and biliary tract infection. METHODS: Thirty-five bile samples and 30 stones specimens were cultured for bacteria and 12 stones specimens were examined with a scan electron microscope (SEM) or a transmission electron microscope (TEM). RESULT: 94.2% bile samples and 96.7% stones specimens were positive in bacteria culture. Bacteria were found in stones under SEM and TEM. CONCLUSION: Bacteria in stones are associated with the infection of the biliary tract. | Shuo-Dong Wu, Hong Yu and Ji-Mei Sun Department of General Surgery Department of Microbiology Center Second Affiliated Hos- pital China Medical University, Shenyang 110004, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，2： | 16 |
| 4 | Diagnostic analysis of hepatic angiomyolipoma显示文摘BACKGROUND: Hepatic angiomyolipoma (HAML) is a rare tumor containing a variable amount of fat, vessels and smooth muscle. We report the image findings on ultra- sonography and computed tomography ( CT) of huge HAML. METHOD; The clinical, imaging and pathological data of a case of HAML were retrospectively collected and ana- lyzed. RESULTS; A huge heterogeneous hyperecho mass with anecho and hypoecho areas inside was found in the left he- patic lobe on ultrasonography. Color Doppler showed blood flow and arterial spectrum in it. CT scan showed a huge heterogeneous solid mass in the left lobe of the liver, with a low density and hypervascular area in arterial phase. The serum tumor marks were all negative. Ultrasound- guided biopsy was taken twice before resection and both showed necrosis tissue and reaction of inflammatory cells. Postoperative pathological results showed that the tumor was composed of epithelioid smooth muscle cells, thick- walled blood vessels and a few adipose cells with necrosis. The immunohistochemistry results showed appea-rance of typical HAML, with HMB-45 positive and alpha fetopro- tein (AFP) negative. CONCLUSIONS: Preoperative diagnosis of HAML relies on combination of CT, MRI and ultrasonography. Our case of HAML showed heterogeneous hyperecho image on ultra- sonography. Ultrasound-guided biopsy combined with morphological manifestation and specimen examination for HMB-45 may be helpful in the diagnosis of HAML. | Tian-An Jiang, Qi-Yu Zhao, Miao-Yan Chen, Li-Jun Wang and Jian-Yang Ao Hangzhou, China Department of Ultrasonography, First Affiliated Hos- pital , Distance Education Center and Department of Pathology, First Affiliated Hospital , Zhejiang University School of Medicine, Hangzhou 310003, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，1： | 4 |
| 5 | Diagnosis and treatment of biliary atresia: a retrospective study显示文摘BACKGROUND: Biliary atresia (BA) is the end results of an inflammatory process, which affect the intrahepatic and extrahepatic bile ducts, leading to fibrosis and obliteration of the biliary tract with the development of biliary cirrho- sis. Early diagnosis of BA is difficult, and there is no speci- fic therapy for BA at present. The purpose of this study was to investigate the diagnosis, treatment and postoperative outcome of BA and to explore new diagnostic and thera- peutic strategies. METHODS; Thirty-one children with biliary atresia from our hospital and 201 children with such disease from other hospitals in China were reviewed retrospectively in terms of diagnostic modalities, operative age, operative methods and long-term survival rates after operation. RESULTS : The operative age of the 31 patients varied from 13 to 270 days. It was <60 days in 6 children (19% ), 61-90 days in 14 (45% ), 91-120 days in 8 (26% ), and >120 days in 3 ( 10% ). No children underwent transplantation for BA. Their 1-6 year survival rate on average was 32. 3% (10/31). The early operative rate in China was 12.9% (30/ 232). One child received liver transplantation with a long- term survival rate of 31.9%. CONCLUSIONS; Early recognition of babies with BA is critical for optimal intervention for preventing progressive fibrosis. Clinical presentation, imaging and laparoscopy are helpful in enhancing the early diagnostic rate of BA pa- tients. The long-term survival rates can be obtained after the improvement of operative methods, liver transplanta- tion for children with advanced BA, and development of other medical strategies. | Shao-Tao Tang, Qing-Lan Ruan, Zhi-Qing Cao, Yong-Zhong Mao, Yong Wang and Shi-Wang Li Wuhan, China Department of Pediatric Surgery, Wuhan Union Hos- pital, Huazhong Science and Technology University Wuhan 430022, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，1： | 3 |
| 6 | Low-concentration sodium hydroxide solution injection in normal liver parenchyma of rabbits显示文摘BACKGROUND: Percutaneous ethanol injection has been widely used as a non-surgical therapy for liver cancer, but it has some shortcomings such as local diffusion and une- qual permeation. This study was designed to observe the volume, controllability and completeness of necrosis after injection of low concentration sodium hydroxide in the normal liver parenchyma so as to assess its possibility in treatment of liver cancer instead of ethanol. METHODS: Twenty-seven New Zealand rabbits were di- vided randomly into 9 groups (Aa, Ab, Ac, Ba, Bb, Bc, Ca, Cb, and Cc) by a 3 × 3 (three-by-three) factorial de- sign, each consisting of 3 rabbits. Group A was given sodi- um hydroxide solution at a concentration of 5%, while B at 2.5% and C at 1% in liver parenchyma. Each group re- ceived three doses of the solution: a (0.2 ml), b (0.5 ml) and c (1.0 ml). Then another 3 rabbits as side-effect group were dropped with sodium hydroxide solution in their liver lobe space. Liver and renal function changes in all the rab- bits were compared after injection with pre-injection. RESULTS: All the lesions were localized. At the concentra- tion of 2.5% and 5%, the lesion volume increased with the dose increased from 0.2 ml to 1.0 ml (P < 0. 05). No sig- nificant differences were found in the lesion volume of the groups receiving the same dose but different concentration. Changes in liver and renal function were not significant 7 days after injection, compared with those before injection. CONCLUSIONS: 2.5% and 5% sodium hydroxide solution could control local complete necrosis in normal liver. With regard to safety, 2.5% alkali solution is considered promis- ing as a new agent for intratumoral injection therapy in- stead of ethanol. | Tian-An Jiang, Qi-Yu Zhao, Miao-Yan Chen and Shu-Sen Zheng Hangzhou, China Department of Ultrasonography, First Affiliated Hos- pital , and Distance Education Center , and Department of Hepatobiliary and Pancreatic Surgery, First Affilia- ted Hospital , Zhejiang University School of Medicine, Hang- zhou 310003, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，2： | 3 |
| 7 | Alcoholism and alcoholic liver disease: focusing on epidemiological investigation in Asia显示文摘 | You-Ming Li Hangzhou, China Department of Gastroenterology, First Affiliated Hos- pital, Zhejiang University School of Medicine, Hangzhou 310003, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，2： | 2 |
| 8 | Management of symptomatic liver cysts显示文摘 | Pitale A Bohra AK Diamond T | 2002 | Ulster Med J2002，71，2： | 1 |
| 9 | Distribution of thoracie lymphadenopathy in sarcoidosis using computed tomography显示文摘 | Pital SN Levin DL | 1999 | J Horae Imaging1999，14，2： | 1 |
| 10 | Distribution of tboracie ly mpbadenopatby in sarcoidosis using computed tomograpby 显示文摘 | Pital S N Levin D L | 1999 | J Horae Imaging1999，14，： | 1 |
| 11 | Testosterone Ireatment enhance regional brain perfusion in hypogonadal men 显示文摘 | Azad N Pitale S Barnes WE | 2003 | J Clin EndocrinolMetab2003，88，7： | 1 |
| 12 | Enhancement of staining intensity in the fluorescent antibody reaction显示文摘 | Pital A Janowitz S L | 1963 | Bacteriology1963，86，： | 1 |
| 13 | 显示文摘 | Sharma S K Pitale S S Malik M M | 2010 | Physica B-Con- densed Matter2010，405，3： | 1 |
| 14 | Renal cell carcinoma with metastasis to the thyroid gland 显示文摘 | Pitale SU Sizemore GW Bakhos R | 2000 | Urol Oncol2000，5，4： | 1 |
| 15 | The relationship between point mutation and abnormal expression of c-fms oncogene in hepatocellular carcinoma显示文摘BACKGROUND: Recent research found abnormal expres- sion of the c-fms oncogene, which encodes the macro- phage colony-stimulating factor receptor (CSF-1R), in several human carcinomas including hepatocellular carcino- ma (HCC). But the relationship between the point muta- tion and abnormal expressing of c-fms oncogene in HCC was not clear. This study is to investigate the relationship between point mutation and abnormal expression of c-fms oncogene in hepatocellular carcinoma (HCC) and to clari- fy the mechanism of HCC. METHODS: The expression of c-fms oncogene at different levels of cell, protein and transcription was observed using immune histological ABC, Western blot and Northern blot. PCR-single strand conformation polymorphism and gene sequencing were used to detect the mutation of c-fms in HCC tissues and their surrounding tissues of 30 patients. RESULTS: The expression of c-fms was significantly higher in HCC tissues than in their surrounding tissues (P <0.01). Point mutation of Leu (TTG)—>Ser (TCG) at codon 301 of c-fms amino acids was observed in 21.4% (3/14) HCC tissues. No mutation of c-fms oncogene was detected in the surrounding cancerous tissues. CONCLUSION: Point mutation at codon 301 of c-fms on- cogene is one of the mechanisms of abnormal over-expres- sion in HCC. | Dong-Hua Yang, Wei Huang, Jun Cui, Jian-Chang Shu, Shao-Hui Tang, Wen-Jie Zhang and Jie-Hua Liang Guangzhou, China Department of Gastroenterology , First Affiliated Hos- pital of Jinan University, Guangzhou 510630, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，1： | 1 |
| 16 | Health-related quality of life in the VA feasibility study on glycemie control and complications in type 2 diabetes mellitus显示文摘 | Pitale S Kernan-Schroeder D Emanuele N | 2005 | J Diabetes Complications2005，19，4： | 1 |
| 17 | Renal cell carcinoma with metastasis to the thyroid gland 显示文摘 | PITALE S U SIZEMORE G W BAKHOS R | 2000 | Urol Oncol2000，5，4： | 1 |
| 18 | Fetal origin of vascular ag-ing显示文摘 | PITALE S SAHASRABUDDHE A | 2011 | Indian J Endocrinol Metab2011，154，： | 1 |
| 19 | Effect of temperature and aluminium on calcium (alumino) silicate hydrate chemistry under equilibrium conditions显示文摘 | MYERS R J L'H?PITAL E PROVIS J L | 2015 | Cem Concr Res2015，68，： | 1 |
| 20 | Genetic va-riety of bovine viral diarrhea virus 1strains isolated fromsheep and goats in India显示文摘 | MISHRA N PITALE S RAJUKUMAR K | 2012 | Acta Virol2012，56，3： | 1 |
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