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| 1 | 副猪嗜血杆菌的分离培养和血清型鉴定显示文摘 从全国十多个省市送检的疑似患多发浆膜炎与关节炎猪的病料中分离到 32株细菌,进行了细菌形态观察、培养特性和生化特性鉴定;根据副猪嗜血杆菌 16SrRNA序列设计引物进行PCR扩增, 将 822bp扩增片段连入T 载体后测序, 再与GenBank(M75065 )中的序列进行比对,表明其与国外副猪嗜血杆菌菌株 16SrRNA序列的同源性为 97%以上,确定为副猪嗜血杆菌(Haemophilusparasuis, Hps)。将其中的 15株按Kieletein Rapp Gabriedson(KRG)琼脂扩散血清分型方法进行血清型鉴定,结果为血清 5型 3株、血清 4型 4株、血清 13型2株、血清 12型 2株,另外有 4株不能进行分型。该结果表明副猪嗜血杆菌在我国广泛存在。 | 蔡旭旺 刘正飞 陈焕春 吴斌 金梅林 Pat Blackall | 2005 | 华中农业大学学报2005，24，1： | 188 |
| 2 | POST-STROKE ANTIHYPERTENSIVE TREATMENT STUDY A PRELIMINARY RESULT显示文摘Post-stroke Antihypertensive Treatment Study (PATS) was a randomized, double-blind and placebo-controlled trial, which aimed at determining whether antihypertensive treatment could reduce the risk of fatal and nonfatal stroke incidence in patients with a history of stroke or transient ischemic attack (TLA). 5 665 patients were randomized by a sealed envelope system. Systolic blood pressure (SBP) ranged from 80 to 280 mm Hg and diastolic (DBP) from 50 to 150 mmHg. The average SBP was 154 mmHg and average DBP 93 mmHg. The mean age was 60 years. Among the patients, women accounted for 28%. In 71% the latest stroke was ischemic. Average follow-up approximated to 2 years. The three-year average SBP was 149 mmHg for the placebo group and 144 mmHg for the indapamide treatment group, and the three-year DBP was 89 mmHg and 87 mmHg respectively. The three-year first incidence of fatal and nonfatal stroke was 12.3 per 100 patients placebo treatment and 9.4 per 100 with indapamide. The relative risk by | PATS Collaborating Group | 1995 | Chinese Medical Journal1995，，9： | 94 |
| 3 | 利用因子分析确定岩溶地下河系统水质的影响因素显示文摘根据云南南洞地下河系统2008年旱季的水化学分析结果,利用因子分析方法探讨地下水水质的影响因素及程度,结果表明:流域地下水成分变动范围较大,具有明显的空间变异性;地下水水质受人类活动、水岩作用过程和土壤等自然因素的共同影响,三个因素能够解释地下水水质形成的79%,其中人类活动是主要影响因素;地下水中的Cl-、NO3-、SO42-、Na+、K+主要受人类活动特别是农业和生活废水等因素的控制,Ca2+主要是水-岩作用的产物,但也受人类活动的影响,pH值、电导率受水岩作用过程和人类活动的影响,Mg2+、HCO3-受土壤CO2含量和水-岩作用过程的共同影响。 | 蒋勇军 吴月霞 Chris Groves 袁道先 Pat Kambesis 李林立 张贵 | 2009 | 水文地质工程地质2009，36，4： | 37 |
| 4 | B型副鸡嗜血杆菌的分离鉴定显示文摘从辽宁某公司鸡场疑似鸡传染性鼻炎的病鸡眶下窦分离到一株副鸡嗜血杆菌 ,用Page程序和Kume程序对其进行血清型鉴定 ,确认为B型副鸡嗜血杆菌 ,这是首次在我国分离到B型副鸡嗜血杆菌。 | 张培君 苗得园 龚玉梅 孙惠玲 Pat Blackall 包爱军 潘裕华 | 2003 | 中国预防兽医学报2003，25，1： | 31 |
| 5 | 猪胸膜肺炎放线杆菌和副猪嗜血杆菌的鉴别诊断及血清型鉴定显示文摘利用PCR技术、琼脂扩散试验、糖发酵试验和间接血凝试验(IHA)对野外分离到的可疑猪胸膜肺炎放线杆菌和副猪嗜血杆菌进行了诊断和血清型鉴定。PCR鉴定分离物HS1580为副猪嗜血杆菌,分离物HS1582为猪胸膜肺炎放线杆菌; 生化试验表明分离物HS1581和HS1582为猪胸膜肺炎放线杆菌; 血清型鉴定分离物HS1580不属于被检的14个血清型之列,HS1581为App血清15型,HS1582为App血清7型。试验结果表明,综合使用PCR等技术可快速、准确地对这两种传染性细菌进行鉴别诊断和血清型鉴定。 | 张培君 孙惠玲 苗得园 龚玉梅 Pat Blackall | 2002 | 中国兽药杂志2002，36，10： | 27 |
| 6 | 产气荚膜梭菌型特异性多重PCR的研究显示文摘 建立了一种简单且具有型特异性的产气荚膜梭菌的多重PCR诊断方法。结果表明,该PCR特异性好,只有产气荚膜梭菌呈现阳性,被检验的其他7种梭菌以及大肠杆菌、葡萄球菌和多杀性巴氏杆菌均为阴性。该PCR具有较高的敏感性,最小检测量为4.2×105CFU/mL,单个菌落稀释10倍后也能被检测出,适用于医院、防疫和兽医诊断等部门,具有重要的公共卫生意义。 | 龚玉梅 Pat Blackall 陈卓明 苗得园 孙惠玲 张培君 | 2004 | 中国兽药杂志2004，38，2： | 11 |
| 7 | Mannhei mia granulo matis PCR检测方法的建立显示文摘Mannheimia granulomatis是导致牛、羊呼吸道传染病的一种病原菌。为快速、准确诊断由该菌导致的疫病,从基因库中获得M.granulomatis的基因序列,再从M.granulomatis的基因序列中获得与其他细菌包括溶血性曼氏杆菌和多杀性巴氏杆菌等不同的基因片段,设计引物,建立了特异性好、敏感性较高的M.granulomatis的PCR诊断方法。结果表明,其特异性为100%,最小检出量为5×104cfu/mL^5×103cfu/mLM.granulomatis或1/10个单个菌落。 | 安烨 龚玉梅 Jeanette Miflin Pat Blackall 孙惠玲 张培君 | 2007 | 动物医学进展2007，28，5： | 8 |
| 8 | Protective effects ofLactobacillus plantarum against epithelial barrier dysfunction of human colon cell line NCM460显示文摘AIM: To investigate the effects of Lactobacillus plantarum (L. plantarum ) in the intestinal permeability and expression of tight junction (TJ) using the normal human colon cell line NCM460. METHODS: Paracellular permeability of NCM460 monolayers was determined by transepithelial electrical resistance and dextran permeability. Expression of TJ proteins in NCM460 cell monolayers was detected by Western blotting and quantitative real-time polymerase chain reaction.RESULTS: L. plantarum played an important role in increasing transepithelial electrical resistance and decreasing the permeability to macromolecules of NCM460 monolayers against the disruption caused by enteropathogenic Escherichia coli (E. coli ) or enteroinvasive E. coli . L. plantarum also prevented the decrease in the expression of TJ proteins and F-actin in NCM460 cells.CONCLUSION: L. plantarum can protect against dysfunction of NCM460 intestinal epithelial barrier caused by enteropathogenic E. coli or enteroinvasive E. coli , and thus can be a potential candidate of therapeutic agents for the treatment of intestinal diseases. | Mary Pat Moyer | 2010 | World Journal of Gastroenterology2010，16，45： | 6 |
| 9 | Epithelial-mesenchymal transition as a therapeutic target for overcoming chemoresistance in pancreatic cancer显示文摘Pancreatic cancer has one of the worst prognoses among all cancers due to the late manifestation of identifiable symptoms and high resistance to chemo- and radiation therapies. In recent years, a cancer development phase termed epithelial-mesenchymal transition(EMT) has gained increasing research focus. The process is implicated in tumour metastasis, and emerging evidence suggests EMT also contributes or induces chemoresistance in several cancers. Nevertheless, the applicability of therapeutic targeting of EMT faces many challenges. In this mini-review, we summarise the evidence supporting the role of EMT in pancreatic cancer progression, focusing particularly on its association with chemoresistance. | Omar Elaskalani Norbaini Binti Abdol Razak Marco Falasca Pat Metharom | 2017 | World Journal of Gastrointestinal Oncology2017，9，1： | 6 |
| 10 | 溶血性曼氏杆菌PCR检测方法的研究显示文摘溶血性曼氏杆菌是导致牛、羊呼吸道传染病的一种病原菌。为快速、准确诊断由本菌导致的疾病 ,从基因库中获得溶血性曼氏杆菌( M.haemolytica )的基因序列 ,再从其基因序列中获得与其它细菌包括 M.annheimiagranulomatis,M.varigena ,M.ruminalis,M.glucosidal ,M.annheimiaspp和多杀性巴氏杆菌等不同的基因片段 ,设计成引物 ,建立了特异性好、敏感性高的 M.haemolytica PCR检测方法。结果表明 ,除溶血性曼氏杆菌为阳性外 ,其余所有细菌均为阴性 ,特异性为 1 0 0 % ,最小检出量为 8× 1 0 2 cfu/ m L 曼氏杆菌或 1 / 1 0个单个菌落。检验人工感染牛 ,检出率为 75%。此PCR检测方法的建立 ,为 M. | 张培君 Jeanette Miflin Pat Blackall 陈卓明 孙惠玲 龚玉梅 苗得园 | 2003 | 动物医学进展2003，24，4： | 6 |
| 11 | 植物基因组重复序列研究进展显示文摘重复序列是指基因组中频繁出现的相同或对称的DNA片段,在植物基因组中占有很大比重。重复序列分为2种类型:一种是串联重复序列,主要分布在染色体着丝粒周缘区域、臂间区域和近端粒区域,某些短的串联重复序列均匀分布在基因组中;另一种是散在重复序列,主要是反转录转座子和DNA转座子,通常散布在染色体或多倍体物种某些(个)亚基因组染色体上。目前重复序列的发现和识别技术已成为基因组学的研究热点,归纳这方面的研究进展,概括相关工具的分析特点,总结重复序列功能研究及其在遗传分析中的应用案例,可为发掘重复序列存在的生物学意义及其在物种亲缘关系分析和种质资源鉴定中的应用奠定基础。 | 李晓宇 徐文魁 Pat Heslop HARRISON 刘青 | 2019 | 扬州大学学报(农业与生命科学版)2019，40，5： | 5 |
| 12 | 胸膜肺炎放线杆菌生物Ⅰ型标准抗血清的制备及初步临床应用显示文摘用十三种血清型的胸膜肺炎放线杆菌标准菌株免疫家兔,制备了标准抗血清,通过玻片凝集试验,琼脂扩散试验和免疫电泳试验,对抗血清的特异性、效价和保存期进行了监测,结果表明,制备的抗血清有较好的特异性,在4℃可以保存一个月、-20℃和、-80℃下保存一年无明显变化。用抗血清对从湖南,安徽,河南等地分离的胸膜肺炎菌株进行分型鉴定,分别为血清2,3,8型;而PCR检测均为阳性,说明抗血清可用于野生株的鉴定和流行病学调查。 | 陈凡 何启盖 陈焕春 刘正飞 张震亚 张迎 Ross Bowle Pat Blackall | 2004 | 中国预防兽医学报2004，26，6： | 5 |
| 13 | 密苏里州立公园ADA指导委员会对公众参与有效性的评价显示文摘本文对美国州立公园设计过程中公众参与的有效性进行了探讨。通过国家公园密苏里分部的有关实例研究,讨论了依据《美国伤残人法》(ADA),市民指导委员会在州立公园体系建设中的作用,以及对公众参与有效性产生影响的因素。 | Pat Crawford 刘健 | 2002 | 国外城市规划2002，17，2： | 5 |
| 14 | 胸膜肺炎放线杆菌诊断抗原的制备及初步临床应用显示文摘利用14个血清型的胸膜肺炎放线杆菌的标准菌株制备诊断抗原,通过玻片凝集试验,琼脂扩散试验和对流免疫电泳,以标准抗血清作为参考,检验各种诊断抗原的灵敏性和特异性。分别用凝集抗原、超声波处理抗原和酚水抗原对来自湖北、湖南、江西、河南、安徽5省猪场的155份送检血清进行检测。结果表明可以对送检血清快速、准确地进行鉴别诊断和血清分型。 | 陈凡 何启盖 陈焕春 吴斌 刘正飞 Ross Bowle Pat Blackall | 2003 | 华中农业大学学报2003，22，5： | 4 |
| 15 | PI controller relay auto-tuning using delay and phase margin in PMSM drives显示文摘This paper presents an auto-tuning method for a proportion plus integral(PI) controller for permanent magnet synchronous motor(PMSM) drives, which is supposed to be embedded in electro-mechanical actuator(EMA) control module in aircraft. The method, based on a relay feedback with variable delay time, explores different critical points of the system frequency response.The Nyquist points of the plant can then be derived from the delay time and filter time constant.The coefficients of the PI controller can then be obtained by calculation while shifting the Nyquist point to a specific position to obtain the required phase margin. The major advantage of the autotuning method is that it can provide a series of tuning results for different system bandwidths and damping ratios, corresponding to the specification for delay time and phase margin. Simulation and experimental results for the PMSM controller verify the performance of both the current loop and the speed loop auto-tuning. | Wang Lina Xiao Kun Liliana de Lillo Lee Empringham Pat Wheeler | 2014 | Chinese Journal of Aeronautics2014，27，6： | 4 |
| 16 | A CASE STUDY OF TEACHING INPUTS AND LEARNING OUTPUTS FOR SPOKEN ENGLISH WITHIN A COLLEGE CONTEXT IN CHINA显示文摘Definition of termsInputs can be defined as everything which students bring tothe classroom including their prior teaching and learningenvironment,attitude,enthusiasm,motivation,phonologyfrom their first language and varying degrees of confidence.When they arrive in college they are exposed to spoken Englishfrom a range of teachers who have a variety of spoken abilitiesand teaching techniques. | Jeremy Brayshaw & Pat Goodall Qinzhou Teachers College,Guangxi | 2004 | Chinese Journal of Applied Linguistics2004，27，5： | 4 |
| 17 | Involvement and therapeutic implications of airway epithelial barrier dysfunction in type 2 inflammation of asthma显示文摘Type 2 inflammation is a complex immune response and primary mechanism for several common allergic diseases including allergic rhinitis,allergic asthma,atopic dermatitis,and chronic rhinosinusitis with nasal polyps.It is the predominant type of immune response against helminths to prevent their tissue infiltration and induce their expulsion.Recent studies suggest that epithelial barrier dysfunction contributes to the development of type 2 inflammation in asthma,which may partly explain the increasing prevalence of asthma in China and around the globe.The epithelial barrier hypothesis has recently been proposed and has received great interest from the scientific community.The development of leaky epithelial barriers leads to microbial dysbiosis and the translocation of bacteria to inter-and sub-epithelial areas and the development of epithelial tissue inflammation.Accordingly,preventing the impairment and promoting the restoration of a deteriorated airway epithelial barrier represents a promising strategy for the treatment of asthma.This review introduces the interaction between type 2 inflammation and the airway epithelial barrier in asthma,the structure and molecular composition of the airway epithelial barrier,and the assessment of epithelial barrier integrity.The role of airway epithelial barrier disruption in the pathogenesis of asthma will be discussed.In addition,the possible mechanisms underlying the airway epithelial barrier dysfunction induced by allergens and environmental pollutants,and current treatments to restore the airway epithelial barrier are reviewed. | Xiang Dong Mei Ding Jinjin Zhang Ismail Ogülür Yagiz Pat Mübeccel Akdis Yadong Gao Cezmi A.Akdis | 2022 | Chinese Medical Journal2022，，5： | 4 |
| 18 | A simulation study of using active traffic management strategies on congested freeways显示文摘The main objective of this study is to evaluate the effectiveness of using active traffic management (ATM) strategies on freeways in terms of the driver's behavior and operational impacts. A few test beds were selected to evaluate the impacts of ATM such as speed harmonization, shoulder utilization, and ramp metering. Test beds used in this study were at places where an ATM is either proposed or previously implemented, i.e., where data exists for conditions prior to and after the implementation of ATM. Data collected from the test beds were used in a simulation model to evaluate the impacts of each ATM strategy on speed, travel time, and crash rates. Simulation results indicated that the implementation of speed harmonization on US 90 showed a 14% reduction in crashes and a 2%-3% increase in freeway speed; the implementation of hard shoulders on US 90 showed a 39% increase in travel time, 22% increase in freeway capacity and 60% decrease in delays; and the implementation of ramp metering on US 59 between Bissonnet St. and Fondern road showed a decrease of 23 % in freeway travel time, a 14% increase in freeway speed and 11% decrease in accident rates. | Ramakrishna VADDE Joseph O. SAI Mohammed A. FARUQI Pat T. LEELANI | 2012 | Journal of Modern Transportation2012，20，3： | 3 |
| 19 | 毛细管电泳分离和激光检测分析多糖胶(英文)显示文摘将多糖胶的混合物与荧光剂 9 氨基芘 1,4 ,6 三磺酸 (APTS)派生后再进行微量离心过滤分离。所得到的高分子部分采用毛细管电泳 (CE)分离和激光诱导荧光 (LIF)检测技术进行分析。缓冲溶液pH的调节和聚丙烯酰胺 (PAA)涂层毛细管的使用有效地改善了多糖胶的分离效率和峰形。在优化条件下 ,iota、kappa角叉菜胶、藻胶、xanthan、carboxymethylcellulose(CMC)等 5种组分的混合物和阿拉伯树胶、刺梧桐树胶、CMC等 3种组分的混合物分别在pH 3 2和 7 8的缓冲溶液下得到了完整组分的分离。 | 彭加瑜 SANDRA Pat | 2004 | 色谱2004，22，3： | 3 |
| 20 | 使用微型模块SIP中的集成无源器件显示文摘简介集成无源器件在我们的行业中并不是什么新事物——它们由来已久且众所周知。关键在于,当芯片组将独立的分立无源器件或者是集成无源网络作为其一部分包含在内时,需要对走线寄生效应、器件兼容性和电路板组装等考虑因素进行仔细的设计管理。虽然集成无源器件继续在业界占据重要地位,但只有当它们被集成到系统级封装应用中时才能实现其最重要的价值。 | Mark Murphy Pat Mc Guinness | 2019 | 中国集成电路2019，0，3： | 3 |
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