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| 1 | 蚕用益生芽孢杆菌SWL-19的筛选鉴定及其对肠道菌群多样性的影响显示文摘【目的】筛选对家蚕(Bombyx mori)具有益生作用的芽孢杆菌,改善家蚕肠道微环境,促进蚕业生产的发展。【方法】采用高温选育法对家蚕肠道内的芽孢杆菌进行初筛;以产胞外消化酶(蛋白酶、脂肪酶、纤维素酶和淀粉酶)及能在肠道定殖为基础,结合溶血试验、抗生素药敏试验和产消化酶能力大小等对芽孢杆菌进行复筛。通过形态、生理生化特征和16S r DNA序列分析鉴定菌种,再利用变性梯度凝胶电泳(DGGE)技术检测其在家蚕肠道内定殖情况及其对肠道内微生物菌群多样性的影响。【结果】共从57株细菌中筛选出两株能同时产蛋白酶、脂肪酶、纤维素酶和淀粉酶且不产生溶血现象的芽孢杆菌SWL-17和SWL-19。14种抗生素药敏纸片检测结果表明,二者对绝大多数抗生素敏感,且敏感程度基本相同。除SWL-19不能利用蔗糖发酵外,两个株菌的革兰氏染色、芽孢染色、产酸产气、柠檬酸利用、葡萄糖发酵、硝酸盐还原和V-P等生理生化特性均相同。二者生长速度相同,但菌落形态特征差异较大。SWL-17的菌落形态为淡黄色,圆形隆起,表面光滑湿润,边缘整齐,而SWL-19的菌落形态为乳白色不透明,表面扁平干燥,边缘不整齐。SWL-17产蛋白酶、脂肪酶、纤维素酶和淀粉酶的能力分别为(1.62±0.04)、(2.12±0.11)、(1.87±0.03)和(1.43±0.03),SWL-19的产酶能力分别为(2.91±0.05)、(2.43±0.04)、(3.24±0.12)和(3.48±0.10)。因此SWL-19具有较高的产消化酶能力,且除脂肪酶外,其产蛋白酶、纤维素酶和淀粉酶的能力均显著性高于SWL-17(P<0.05)。细菌16S r DNA序列比对和系统发育分析表明SWL-17和SWL-19分别为芽孢杆菌(Bacillus sp.)和枯草芽孢杆菌(B.subtilis),且SWL-19具有更清楚的遗传背景,适于进行后续试验。进一步DGGE试验结果表明,自然条件下家蚕肠道内的优势菌群为肠球菌属(Enterococcus)细菌,而外源性添加菌株SWL-19后能使家蚕肠道内的芽孢杆菌属和肠杆菌属细菌成为优势菌群,说明芽孢杆菌SWL-19能在家蚕肠道内定殖,并且能改变家蚕肠道内微生物菌群的结构和多样性。【结论】家蚕益生芽孢杆菌的筛选为研发蚕用复合微生态制剂提供菌株资源,在蚕业生产上具有重要的应用价值。 | 李冠楠 夏雪娟 SENDEGEYA Parfait 何石宝 郭东东 朱勇 | 2015 | 中国农业科学2015，48，9： | 3 |
| 2 | 氟化物对家蚕耐氟和氟化物敏感品种幼虫中肠羧酸酯酶及全酯酶活性的影响显示文摘为了探讨氟化物在家蚕Bombyx mori体内的代谢途径,以家蚕耐氟品种T6和氟化物敏感品种734为研究材料,在5龄幼虫1-7d内分别添食经50,100,200和400mg/kg NaF溶液浸泡后的新鲜桑叶,检测家蚕中肠羧酸酯酶(CarE)和全酯酶活性的变化。结果表明:734添氟组的CarE活性是对照组的1.21~1.98倍,而T6添氟组约是对照组的0.72~1.10倍。734和T6添氟组的全酯酶活性数值变化规律与其各自对照组相似,且2品种之间的酶活性数值很相近。2品种在相同浓度下,不同天数之间的全酯酶活性差异均显著(P<0.05)。推测氟化物对敏感家蚕中肠CarE有促进作用,对耐氟家蚕中肠CarE有抑制作用,但是对全酯酶活性影响不大。 | 米智 阮成龙 李姣蓉 付巧娟 武婧洁 SENDEGEYA Parfait 朱勇 | 2013 | 昆虫学报2013，56，5： | 2 |
| 3 | Neurofibromatosis type 1 molecular diagnosis:what can NGS do for you when you have a large gene with loss of function mutations显示文摘 | Pasmant E Parfait B Luscan A | 2015 | Eur J Hum Genet2015，23，5： | 1 |
| 4 | Bananas, raw materials for making processed food products显示文摘 | Aurore G Parfait B Fahrasmane L | 2009 | Trends Food Sci Technol2009，，20： | 1 |
| 5 | Compound heterozygous mutations in the flavoprotein gene of the respiratory chain complex Ⅱ in a patient with Leigh syndrome显示文摘 | Parfait B Chretien D Rotig A Marsac C Munnich A Rustin P | 2000 | Hum Genet2000，106，2： | 1 |
| 6 | Quantification of estrogen receptor alpha and bern expression in sporadic bresst cancer显示文摘 | Bieche I Parfait B Laurendeau I | 2001 | Oncogene2001，20，56： | 1 |
| 7 | Neurofibromatosis type 1 molecular diagnosis:what can NGS do for you when you have a large gene with loss of function mutations?显示文摘 | Pasmant E Parfait B Luscan A | 2015 | Eur J Hum Genet2015，23，5： | 1 |
| 8 | Bananas,raw materials for making processed food products显示文摘 | Aurore G Parfait B Fahrasmane L | 2009 | Trends Food Sci Technol2009，，20： | 1 |
| 9 | Bananas, raw materials for making processed food products 显示文摘 | Aurore G Parfait B Fahrasmane L | 2009 | Trends in Food Science k Technology2009，20，2： | 1 |
| 10 | Non-coding RNAs:Role in diabetic foot and wound healing显示文摘Diabetic foot ulcer(DFU)and poor wound healing are chronic complications in patients with diabetes.The increasing incidence of DFU has resulted in huge pressure worldwide.Diagnosing and treating this condition are therefore of great importance to control morbidity and improve prognosis.Finding new markers with potential diagnostic and therapeutic utility in DFU has gathered increasing interest.Wound healing is a process divided into three stages:Inflammation,proliferation,and regeneration.Non-coding RNAs(ncRNAs),which are small protected molecules transcribed from the genome without protein translation function,have emerged as important regulators of diabetes complications.The deregulation of ncRNAs may be linked to accelerated DFU development and delayed wound healing.Moreover,ncRNAs can be used for therapeutic purposes in diabetic wound healing.Herein,we summarize the role of microRNAs,long ncRNAs,and circular RNAs in diverse stages of DFU wound healing and their potential use as novel therapeutic targets. | Yi-Bo Tang Muhuza Marie Parfaite Uwimana Shu-Qi Zhu Li-Xia Zhang Qi Wu Zhao-Xia Liang | 2022 | World Journal of Diabetes2022，13，12： | 1 |
| 11 | Bananas, raw materialsfor making processed food products 显示文摘 | Aurore G Parfait B Fahrasmane L | 2009 | Trends in FoodScience & Technology2009，20，2： | 1 |
| 12 | Quantitation of androgen receptor gene expression in sporadic breast tumors by real-time RT-PCR: evidence that MYC is an AR-regulated gene显示文摘 | Bieche I Parfait B Tozlu S | 2001 | Carcinogenesis2001，22，9： | 1 |
| 13 | Bananas, raw materials for making processed food products 显示文摘 | Aurore G Parfait B Fahrasmane L | 2009 | Trends Food SciTechnol2009，20，： | 1 |
| 14 | Bananas, raw materials for making processed food products 显示文摘 | Guylene Aurore Berthe Parfait Louis Fahrasmane | 2009 | Food Science and Technology2009，，20： | 1 |
| 15 | Compound heterozygous mutations in the flavoprotein gene of the respiratory chain complex II in a patient with Leigh syndrome显示文摘 | Parfait B Chretien D Rotig A | 2000 | Hum Genet2000，106，： | 1 |
| 16 | Quantification of estrogen receptor alpha and beta expression in sporadic breast cancer显示文摘 | Parfait B Laurendeau I | 2001 | Oncogene2001，20，56： | 1 |
| 17 | Bananas,raw materials for making processed food products 显示文摘 | Aurore G Parfait B Fahrasmane L | 2009 | Trends in Food Science & Technology2009，20，2： | 1 |
| 18 | Quantification of estrogen receptor alpha and beta expression in sporadic breast cancer 显示文摘 | Bieche I Parfait B Laurendeau I | 2001 | Oncegene2001，20，56： | 1 |
| 19 | Bananas, raw materials for making processed food products显示文摘 | Aurore G Parfait B Fahrasmane L | 2009 | Trends in Food Science & Technology2009，20，2： | 1 |
| 20 | Quantification of estro- gen receptor alpha and beta expression in sporadic breast cancer 显示文摘 | Biche I Parfait B Laurendeau I | 2001 | Oncogene2001，20，56： | 1 |
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