|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Hemozoin triggers tumor necrosis factor alpha-mediated release of lysozyme by human adherent monocytes:new evidences on leukocyte degranulation in P.falciparum malaria显示文摘Objective:Avidly phagocytosed hemozoin(malarial pigment) alters several functions of human monocytes and stimulates generation of several cytokines.Recently,we showed that phagocytosis of hemozoin by human monocytes increases expression and activity of matrix metalloproteinase-9,a proteolytic enzyme available in specific gelatinase granules,which contain several enzymes including lysozyme.Present work investigated active lysozyme release after phagocytosis of hemozoin and its dependence on production of tumor necrosis factor alpha. Methods:After phagocytosis of hemozoin,hemozoin-containing trophozoites or control meals(opsonized nonparasitized red blood cells and latex particles),monocyte supematants were monitored for 2 hours,in presence of blocking anti-human tumor necrosis factor alpha antibodies or recombinant human tumor necrosis factor alpha cytokine in selected experiments.Lysozyme release was evaluated by a specific spectrometric assay measuring lysozyme activity after coincubation of cell supematants with suspensions of Mycrococcus Lysodeikticus,while levels of soluble tumor necrosis factor alpha were analyzed by specific enzyme-linked immunodsorbent assay. Results:Levels of lysozyme activity and soluble tumor necrosis factor alpha protein were increased in hemozoin in-or trophozoites-laden monocytes supematants.Phagocytosis per se(control meals) also increased lysozyme release,but levels were significantly lower than those obtained after phagocytosis of hemozoin or trophozoites. Interestingly,all effects on lysozyme release observed after phagocytosis were abrogated by blocking anti-human tumor necrosis factor alpha antibodies,while they were mimicked by recombinant human tumor necrosis factor alpha cytokine.Conclusions:Present work shows that phagocytosis of hemozoin promotes monocyte degranulation and enhances active lysozyme release.The effect requires tumor necrosis factor alpha mediation. | Mauro Prato Giribaldi G Arese P | 2009 | Asian Pacific Journal of Tropical Medicine2009，2，3： | 3 |
| 2 | Stochastic Viability and Invariance显示文摘 | Aubin J P Da Prato G | 1990 | Annali Scuola Normale Di Pisa1990，17，： | 1 |
| 3 | Angew Chem lnt Ed 显示文摘 | Guldi D M Rahman G M A Prato M | 2005 | 44(13): 20152005，44，13： | 1 |
| 4 | Factors affecting the healing response of the intrabony defects following guided tissue regeneration and access flap surgery 显示文摘 | Tonetti MS Pini Prato G Cortellini P | 1996 | J Clin Periodon- tol1996，23，： | 1 |
| 5 | Intention to usebike-sharing for holiday cycling : An application of the theory of plannedbehavior显示文摘 | KAPLAN S MANCA F NIELSEN T PRATO C G | 2015 | Tourism Management2015，47，4： | 1 |
| 6 | Stochastic Cahn-Hilliard equation显示文摘 | Debussche A | 1996 | Nonlinear Analysis1996，26，2： | 1 |
| 7 | Periodontal regeneration of human intrabony defects with titanium reinforced membranes显示文摘 | Cortellini P Pini Prato G Tonetti MS | 1995 | Periodontol1995，66，9： | 1 |
| 8 | Selecting arming systems u- sing a new multiple criteria decision model: The balancingand ranking method显示文摘 | STRASSERT G PRATO T | 2002 | Ecological Economics2002，40，2： | 1 |
| 9 | Europium(III)cryptate:a fluo- rescent label for the detection of DNA hybrids on solid sup- port显示文摘 | PratO Lopez E Mathis G | 1991 | Anal Biochem1991，195，： | 1 |
| 10 | Half-dose depot triptorelin in pituitary suppression for multiple ovarian stimulation in assisted reproduction technology:a randomized study显示文摘 | Dal Prato L Borini A Coticchio G | 2004 | Hum Reprod2004，19，： | 1 |
| 11 | Determining the extent to which delayed-enhancement images reflect the partition-coefficient of Gd-DTPA in canine studies of reperfused and unreperfused myocardial infarction显示文摘 | Thornhill RE Prato FS Wisenberg G | 2004 | Magn Reson Med2004，52，5： | 1 |
| 12 | Periodic and Almost Periodic Solutions for Semilinear Stochastic Equations显示文摘 | Prato G Da Tudor C | 1995 | Stochastic Analysis and Applications1995，3，1： | 1 |
| 13 | Quantification of myocardial blood flow and extracellular volumes using a bolus injection of Gd-DTPA:kinetic modeling in canine ischemic disease显示文摘 | Disbourg LD Prato FS Wisenberg G | 1992 | Magn Reson Imaging1992，23，： | 1 |
| 14 | Optimal therapy of type 2 diabetes: a controversial challenge 显示文摘 | Dardano A Penno G Del Prato S | 2014 | Aging ( Albany NY )2014，6，3： | 1 |
| 15 | Linear i ntegro differential equations in Banach spaces显示文摘 | Da Prato G Ianelli M | 1980 | Rend Mat Univ Padova1980，62，： | 1 |
| 16 | Chemlnforin abstiact:Additionof azomethine ylides to C60:synthesis,characterization,andfunctionalization of fullerene pyrrolidines显示文摘 | Maggini M Scorrano G Prato M | 1994 | J Am Chem Soc1994，25，5： | 1 |
| 17 | The determination ofmyocardial viability using Gd-DTPA in a canine model of acutemyocardial ischemia and reperfusion显示文摘 | Pereira R S Prato F S Wisenberg G | 1996 | Magn Reson Med1996，36，5： | 1 |
| 18 | Organic functionalization of carbon nanotubes显示文摘 | Vasilios G Kordatos K Prato M ct al | 2002 | Journal of the American Chemical Society2002，124，5： | 1 |
| 19 | Half-dose depot triptorelin in pituitary suppression for multiple ovarian stimulation in assisted reproduction technology:a randomized study显示文摘 | Dal Prato L Borini A Coticchio G | 2004 | Hum Reprod2004，19，10： | 1 |
| 20 | Adrenomedullin and ocular inflammation in the rabbit显示文摘 | Clementi G Floriddia ML Prato A | | 0，，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员