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| 1 | Double-stranded DNA in exosomes: a novel biomarker in cancer detection显示文摘 | Basant Kumar Thakur Haiying Zhang Annette Becker Irina Matei Yujie Huang Bruno Costa-Silva Yan Zheng Ayuko Hoshino Helene Brazier Jenny Xiang Caitlin Williams Ruth Rodriguez-Barrueco Jose M Silva Weijia Zhang Stephen Hearn Olivier Elemento Navid Paknejad Katia Manova-Todorova Karl Welte Jacqueline Bromberg Hector Peinado David Lyden | 2014 | Cell Research2014，24，6： | 98 |
| 2 | Interplay between post-translational cyclooxygenase-2 modifications and the metabolic and proteomic profile in a colorectal cancer cohort显示文摘BACKGROUND Colorectal cancer(CRC) is the second most common cause of cancer death worldwide. It is broadly described that cyclooxygenase-2(COX-2) is mainly overexpressed in CRC but less is known regarding post-translational modifications of this enzyme that may regulate its activity, intracellular localization and stability. Since metabolic and proteomic profile analysis is essential for cancer prognosis and diagnosis, our hypothesis is that the analysis of correlations between these specific parameters and COX-2 state in tumors of a high number of CRC patients could be useful for the understanding of the basis of this cancer in humans.AIM To analyze COX-2 regulation in colorectal cancer and to perform a detailed analysis of their metabolic and proteomic profile.METHODS Biopsies from both healthy and pathological colorectal tissues were taken under informed consent from patients during standard colonoscopy procedure in the University Hospital of Bellvitge(Barcelona, Spain) and Germans Trias i Pujol University Hospital(Campus Can Ruti)(Barcelona, Spain). Western blot analysis was used to determine COX-2 levels. Deglycosylation assays were performed in both cells and tumor samples incubating each sample with peptide N-glycosidase F(PNGase F). Prostaglandin E2(PGE2) levels were determined using a specific ELISA. 1 H high resolution magic angle spinning(HRMAS) analysis was performed using a Bruker AVIII 500 MHz spectrometer and proteomic analysis was performed in a nano-liquid chromatography-tandem mass spectrometer(nano LC-MS/MS) using a QExactive HF orbitrap MS.RESULTS Our data show that COX-2 has a differential expression profile in tumor tissue of CRC patients vs the adjacent non-tumor area, which correspond to a glycosylated and less active state of the protein. This fact was associated to a lesser PGE2 production in tumors. These results were corroborated in vitro performing deglycosylation assays in HT29 cell line where COX-2 protein profile was modified after PNGase F incubation, showing higher PGE2 levels. Moreover,HRMAS analysis indicated that tumor tissue has altered metabolic features vs non-tumor counterparts, presenting increased levels of certain metabolites such as taurine and phosphocholine and lower levels of lactate. In proteomic experiments, we detected an enlarged number of proteins in tumors that are mainly implicated in basic biological functions like mitochondrial activity,DNA/RNA processing, vesicular trafficking, metabolism, cytoskeleton and splicing.CONCLUSION In our colorectal cancer cohort, tumor tissue presents a differential COX-2 expression pattern with lower enzymatic activity that can be related to an altered metabolic and proteomic profile. | Patricia Prieto Rafael I Jaén Daniel Calle María Gómez-Serrano Estefanía Nú?ez María Fernández-Velasco Paloma Martín-Sanz Sergio Alonso Jesús Vázquez Sebastián Cerdán Miguel ángel Peinado Lisardo Boscá | 2019 | World Journal of Gastroenterology2019，25，4： | 4 |
| 3 | Comparative study of aromatic compounds in two young white wines subjected to pre-fermentative cryomaceration显示文摘 | Rafael A. Peinado Juan Moreno Juan E. Bueno Jose A. Moreno Juan C. Mauricio | 2003 | Food Chemistry2003，，4： | 2 |
| 4 | Free radical macrophotoinitiators: an overview on recent advances显示文摘 | Corrales T Catalina F Peinado C | 2003 | Journal of Photochemistry and Photobiology A: Chemistry2003，159，2： | 1 |
| 5 | The secreted factors responsi-ble for pre-metastatic niche formation:old sayings and newthoughts显示文摘 | Peinado H Lavotshkin S Lyden D | 2011 | Semin Cancer Biol2011，21，2： | 1 |
| 6 | Determination of the antibacterial drug sulfamethoxazole in pharmaceutical preparations containing trimethoprim by spectrofluorimetry after derivatization with fluorescamine显示文摘 | Blanco CC Carretero SA Peinado FS | 1999 | Fresenius J Anal Chem1999，365，5： | 1 |
| 7 | Snail, Zeb and bHLH fac- tors in tumour progression: An alliance against the epithehal phenotype? 显示文摘 | Peinado H Olmeda D Cano A | 2007 | Nat Rev Cancer2007，7，6： | 1 |
| 8 | Snail,Zeb and bHLH factors intumour progression : an alliance against the epithelial phenotype?显示文摘 | Peinado H Olmeda D Cano A | 2007 | Nat Rev Cancer2007，7，6： | 1 |
| 9 | The transcription factor Slug represses E-cadherin expression andinduces epithelial tomesenchymal transitions:acomparison with Snail and FA7 repressors显示文摘 | Bolos V Peinado H P6rez-Moreno MA etal | 2002 | J Cell Sci2002，116，3： | 1 |
| 10 | Antioxidant activity ofdifferent phenolics fractions isolated in must from Pedro Ximenez grapesat different stages of the off-vine drying process显示文摘 | PEINADO J LERMA N L MORENO J | 2009 | Food Chemistry2009，114，3： | 1 |
| 11 | Lysyl oxidase-like 2 as a new poor prognosis marker of squamous cell carcinomas显示文摘 | Peinado H Moreno-Bueno G Hardisson D | 2008 | Cancer Res2008，68，12： | 1 |
| 12 | Comparative study of aromatic compounds in two young white wines sub- jected to pre-fermentative cryomaceration显示文摘 | Peinado RA Moreno J Bueno JE | 2004 | Food Chemistry2004，84，： | 1 |
| 13 | Rhythms in pineal immunoreactive somatostatin in the Syrian hamster, mouse and gerbil 显示文摘 | Webb S M Peinado M A Puig-Domingo M | 1988 | J Pineal Res1988，5，3： | 1 |
| 14 | Inflammatory reaction in pulmonary muscular arteries of patients with mild chronic obstructive pulmonary disease 显示文摘 | Peinado VI Barbera JA Abate P | 1999 | Am J Respir Crit Care Med1999，159，： | 1 |
| 15 | The transcription factor Slug represses E-cadherin expression and induces epithelial to mesenchymal transitions:A comparison with Snail and E47 repressors显示文摘 | Bolos V Peinado H Perez-Moreno MA | 2003 | J Cell Sc2003，116，： | 1 |
| 16 | Antioxidant activity, browning and volatile Maillard compounds in Pedro Xim enez sweet wines under accelerated oxidative aging显示文摘 | LERMA N L PEINADO J MORENO J | 2010 | LWT - Food Science and Technology2010，43，10： | 1 |
| 17 | Lysyl oxidase - like 2 as a new poor prognosis marker of squamous cell carcinomas 显示文摘 | Peinado H Moreno- Bueno G Hardisson D | 2008 | Cancer Res2008，68，12： | 1 |
| 18 | Phosphorylation of osteopontin is required for inhibition of vascular smooth muscle cell calcification显示文摘 | Jono S Peinado C Giachelli CM | 2000 | Biol Chem2000，275，20： | 1 |
| 19 | Transcriptional regulation of cadherins during develotmaent and carcinogenesis 显示文摘 | Peinado H Portillo F Carlo A | 2004 | lnt J Dev Biol2004，48，36： | 1 |
| 20 | Snail, Zeb and bHLH factors in tumour progression: an alliance against the epithelial phenotype 显示文摘 | Peinado H Olmeda D Cano A | 2007 | Nat Rev Cancer2007，7，6： | 1 |
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