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| 1 | Evaluation of a multiplex PCR assay for detection of cytomegalovirus in stool samples from patients with ulcerative colitis显示文摘AIM: To evaluate a multiplex PCR assay for the detection of bacterial and viral enteropathogens in stool samples from patients with ulcerative colitis(UC). METHODS: We prospectively analyzed 300 individuals, including immunocompetent patients, immunocompromised patients, and patients with UC. Stool samples were collected from the recto-sigmoid region of the colon by endoscopy. The samples were qualitatively analyzed for bacterial and viral enteropathogens with a multiplex PCR assay using a Seeplex® Kit. Additional clinical and laboratory data were collected from the medical records. RESULTS: A multiplex PCR assay detected 397 pathogens(191 bacteria and 206 viruses) in 215 samples(71.7%). The most frequently detected bacteria were Escherichia coli H7, 85(28.3%); followed by Aeromonas spp., 43(14.3%); and Clostridium perfringens, 36(12.0%) samples. The most prevalent viruses were Epstein-Barr virus(EBV), 90(30.0%); followed by human herpes virus-6(HHV-6), 53(17.7%); and cytomegalovirus(CMV), 37(12.3%) samples. The prevalence rate of CMV infection was significantly higher in the immunocompromised group than in the immunocompetent group(P < 0.01). CMV infection was more common in patients with UC(26/71; 36.6%)than in the immunocompetent patients excluding UC(6/188; 3.2%)(P < 0.01). CMV infection was more prevalent in UC active patients(25/58; 43.1%) than in UC inactive patients(1/13; 7.7%)(P < 0.05). Among 4 groups which defined by the UC activity and immunosuppressive drugs, the prevalence rate of CMV infection was highest in the UC active patients with immunosuppressive drugs(19/34; 55.8%). EpsteinBarr virus(EBV) infection was more common in the immunocompromised patients excluding UC(18/41; 43.9%) than in the immunocompetent patients excluding UC(47/188; 25.0%)(P < 0.05). The simultaneous presence of CMV and EBV and/or HHV6 in UC active patients(14/58; 24.1%) was greater than in immunocompromised patients excluding UC(5/41; 12.2%)(P < 0.05). CONCLUSION: The multiplex PCR assay that was used to analyze the stool samples in this study may serve as a non-invasive approach that can be used to exclude the possibility of CMV infection in patients with active UC who are treated with immunosuppressive therapy. | Saifun Nahar Atsushi Iraha Akira Hokama Ayako Uehara Gretchen Parrott Tetsuya Ohira Masatoshi Kaida Tetsu Kinjo Takeshi Kinjo Tetsuo Hirata Nagisa Kinjo Jiro Fujita | 2015 | World Journal of Gastroenterology2015，21，44： | 4 |
| 2 | fl-amino alcohols derived from ( IR, 2S )-norephedrine and ( 1S, 2S )-pseudonorephedrine as catalysts in the asymmetric addition of diethylzine to aldehydes显示文摘 | PARROTT R W HITCHCOCK S R | 2008 | Tetrahedron: Asymmetry2008，19，1： | 1 |
| 3 | Prediction of intestinal absorption: comparative assessment of GASTROPLUS and IDEA显示文摘 | Parrott N Lave T | 2002 | Eur J Pharm Sci2002，17，12： | 1 |
| 4 | Design and evaluation of an oral controlled release delivery system for melatonin in human subjects显示文摘 | LEE BJ PARROTT KA AYRES JW | 1995 | Int J Pharm1995，124，2： | 1 |
| 5 | Laser Beam Welding is Ready to Go to Work at Cadillac显示文摘 | Forbis parrott C A | 1991 | Welding Journal1991，，7： | 1 |
| 6 | Generalized Galerkin methods for first order hyperbolic equations 显示文摘 | MORTON K W PARROTT A K | 1980 | Journal of Computational Physics1980，36，： | 1 |
| 7 | Stimulation of feeding in the satiated pig by intracerebroventricular injection of NPY显示文摘 | Parrott R T Heavens R P Baldwin B A | 1986 | Physiology and Behavori1986，36，： | 1 |
| 8 | Expression and action of kit ligand/stem cell factor in normal human and bovine ovarian surface epithelium and ovarian cancer 显示文摘 | Parrott JA Kim G Skinner MK | 2000 | Biology of Reproduction2000，62，6： | 1 |
| 9 | Theory, explanation, and a third generation of theoretical development in social gerontology显示文摘 | Bengtson V L Burgess Ig O Parrott T M | 1997 | The Journal of Gerontology Series B Psychological Sciences and Social Sciences1997，52，2： | 1 |
| 10 | A physiologically based pharmacoki netic model of the minipig: data compilation and model implemen- tation显示文摘 | Suenderhauf C Parrott N | 2013 | Pharm Res2013，30，1： | 1 |
| 11 | Terahertz Pulsed Imaging in Vivo: Measurements andProcessing Methods 显示文摘 | Parrott Edward P J Sy Stanley M Y Blu Thierry | 2011 | Journal of Biomedical Optics(S1083-3668)2011，16，10： | 1 |
| 12 | Genotype effects on pro-liferative embryogenesis and plant regeneration of soybean显示文摘 | Bailey M A Boerma H R Parrott W A | 1993 | InVitro Cellular & Developmental Biology-Plant1993，29，： | 1 |
| 13 | Diffusion in cementitious materials: Further investigations of chloride and oxygen diffusion in well-cured OPC and OPC 30% PFA plasters显示文摘 | Ngala V T Page C L Parrott L J | 1995 | Cement and Concrete Research1995，25，4： | 1 |
| 14 | Bacterial endotoxin-induced gene expression in the choroid plexus and paraventricular and supraopatic hy pothamic nuclei of the sheep 显示文摘 | Vellucci SV Parrott RF | 1996 | Brain Res Mol Res1996，43，12： | 1 |
| 15 | Preparation and purification of lymphocytes from the epithelium and lamina propria of murine small intestine显示文摘 | Davies MD Parrott DM | 1981 | Gut1981，22，6： | 1 |
| 16 | Effect of polyethylene glycol and sugar alcohols on soybean somatic 显示文摘 | Walker D R Parrott W A | 2001 | Plant Cell Tissue and Organ Culture2001，64，55： | 1 |
| 17 | Villous atrophy and expulsion of intestinal Trichinella spiralis are mediated by T cells 显示文摘 | Manson-Smith DF Bruce RG Parrott DMV | 1979 | Cell Immunol1979，47，2： | 1 |
| 18 | Hypertension management in adults with diabetes 显示文摘 | Arauz-Pacheco C Parrott MA Raskin P | 2004 | Diabetes Care2004，27，1： | 1 |
| 19 | The NICU graduate: implications for pediatric primary care 显示文摘 | Newnam K Parrott J | 2013 | Newborn Infant Nurs Rev2013，13，2： | 1 |
| 20 | Correlations between extent of X-ray infiltration and levds of serum C reactive pro-tein in adult non-severe community-acquired pneumonia 显示文摘 | Tamayose M Fujita J Parrott G | 2015 | J Infect Chemother2015，2，6： | 1 |
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