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| 1 | P53-mediated cell cycle arrest and apoptosis through a caspase-3-independent,but caspase-9-dependent pathway in oridonin-treated MCF-7 human breast cancer cells显示文摘瞄准:在 vitro 在导致 oridonin 的 MCF-7human 乳癌房间 apoptosis 学习 caspase-3-independent 机制。方法:oridonintreated MCF-7 房间的生存能力被 MTT (thiazole 蓝色) 测量试金。有压缩原子核的 Apoptotic 房间被阶段对比显微镜学设想。Nucleosomal DNA 破碎是由统帅玫瑰胶化电气泳动的 assayed。apoptotic 比率被决定由喂奶脱氢酶试金。房间周期引申 andmitochondrial 膜潜力被流动 cytometric 分析测量。Bax, Bcl-2, caspase-3, caspase-9,热吃惊蛋白质(Hsp ) 90, p53, p-p53, p21,(自动数据处理核糖) Poly,聚合酶(PARP ) ,和激活 caspase 的 DNase (ICAD ) 蛋白质表情的禁止者被西方的污点分析检测。结果:Oridonin 在一个时间依赖者和剂量依赖者举止禁止了房间生长。房间周期被改变通过起来 p53 和 p21 蛋白质表情的规定。Pancaspase inhibitorZ-VAD-fmk 和 calpain 禁止者 II 两个都减少了房间死亡比率。Nucleosomal DNA 破碎并且下面 DeltaPhimit 的规定在导致 oridonin 的 MCF-7 房间 apoptosis 被检测,它涉及一条 postmitochondrial caspase-9-dependent 小径。减少的 Bcl-2 和 Hsp90expression 层次和 p21 表示层次断然与 phosphorylated p53 phosphorylation 的提高的层次被相关。而且, PARP 部分被劈开 bycalpain 而非由 capase-3。结论:DNA 损坏在线粒体陆地 caspase-9 小径以及调停 p53 的房间周期拘捕挑起了引申,但是不与在导致 oridonin 的 MCF-7 房间的 caspase-3activity 有关。 | Qiao CUI Jing-hua YU Jin-nan WU Shin-ichi TASHIRO Satoshi ONODERA Mutsuhiko MINAMI Takashi IKFJIMA | 2007 | Acta Pharmacologica Sinica2007，28，7： | 30 |
| 2 | Role of autophagy in the pathogenesis of inflammatory bowel disease显示文摘Inflammatory bowel disease(IBD) results from a complex series of interactions between susceptibility genes, the environment, and the immune system.Recently, some studies provided strong evidence that the process of autophagy affects several aspects of mucosal immune responses. Autophagy is a cellular stress response that plays key roles in physiological processes, such as innate and adaptive immunity, adaptation to starvation, degradation of aberrant proteins or organelles, antimicrobial defense, and protein secretion. Dysfunctional autophagy is recognized as a contributing factor in many chronic inflammatory diseases, including IBD. Autophagy plays multiple roles in IBD pathogenesis by altering processes that include intracellular bacterial killing, antimicrobial peptide secretion by Paneth cells, goblet cell function, proinflammatory cytokine production by macrophages, antigen presentation by dendritic cells, and the endoplasmic reticulum stress response in enterocytes. Recent studies have identified susceptibility genes involved in autophagy, such as NOD2, ATG16L1, and IRGM, and active research is ongoing all over the world. The aim of this review is a systematic appraisal of the current literature to provide a better understanding of the role of autophagy in the pathogenesis of IBD. Understanding these mechanisms will bring about new strategies for the treatment and prevention of IBD. | Tomoya Iida Kei Onodera Hiroshi Nakase | 2017 | World Journal of Gastroenterology2017，23，11： | 27 |
| 3 | Retrospective cohort study Lower incidence of complications in endoscopic nasobiliary drainage for hilar cholangiocarcinoma显示文摘AIM:To identify the most effective endoscopic biliary drainage technique for patients with hilar cholangiocarcinoma.METHODS:In total,118 patients with hilar cholangiocarcinoma underwent endoscopic management[endoscopic nasobiliary drainage(ENBD)or endoscopic biliary stenting]as a temporary drainage in our institution between 2009 and 2014.We retrospectively evaluated all complications from initial endoscopic drainage to surgery or palliative treatment.The risk factors for biliary reintervention,post-endoscopic retrograde cholangiopancreatography(post-ERCP)pancreatitis,and percutaneous transhepatic biliary drainage(PTBD)were also analyzed using patient-and procedure-related characteristics.The risk factors for bilateral drainage were examined in a subgroup analysis of patients who underwent initial unilateral drainage.RESULTS:In total,137 complications were observed in92(78%)patients.Biliary reintervention was required in 83(70%)patients.ENBD was significantly associated with a low risk of biliary reintervention[odds ratio(OR)=0.26,95%CI:0.08-0.76,P=0.012].Post-ERCP pancreatitis was observed in 19(16%)patients.An absence of endoscopic sphincterotomy was significantly associated with post-ERCP pancreatitis(OR=3.46,95%CI:1.19-10.87,P=0.023).PTBD was required in 16(14%)patients,and Bismuth type III or IV cholangiocarcinoma was a significant risk factor(OR=7.88,95%CI:1.33-155.0,P=0.010).Of 102 patients with initial unilateral drainage,49(48%)required bilateral drainage.Endoscopic sphincterotomy(OR=3.24,95%CI:1.27-8.78,P=0.004)and Bismuth II,III,or IV cholangiocarcinoma(OR=34.69,95%CI:4.88-736.7,P<0.001)were significant risk factors for bilateral drainage.CONCLUSION:The endoscopic management of hilar cholangiocarcinoma is challenging.ENBD should be selected as a temporary drainage method because of its low risk of complications. | Kazumichi Kawakubo Hiroshi Kawakami Masaki Kuwatani Shin Haba Taiki Kudo Yoko A Taya Shuhei Kawahata Yoshimasa Kubota Kimitoshi Kubo Kazunori Eto Nobuyuki Ehira Hiroaki Yamato Manabu Onodera Naoya Sakamoto | 2016 | World Journal of Gastrointestinal Endoscopy2016，8，9： | 26 |
| 4 | Pseudolaric acid B induces apoptosis,senescence,and mitotic arrest in human breast cancer MCF-7显示文摘目的:现在的学习的目的是在人的乳癌 MCF-7 cells.Methods:3-( 4,5-dimethylthiazol-2-yl )上调查 pseudolaric 酸 B ( PAB )的禁止的效果 -2,5-diphenyltetrazolium 溴化物分析,词法变化,染色的吖啶橙,并且统帅玫瑰胶化电气泳动被使用检测 apoptotic 的 apoptosis.The 百分比,坏死的房间被lactate 脱氢酶计算基于活动的细胞毒性试金;联系的老朽( SA ) -beta-galactosidase 活动被检测评估老朽; propidium 碘化物染色的流动 cytometric 分析被执行调查房间周期的分发,并且蛋白质表示被西方的污点 analysis.Results 检验:在 apoptosis 期间,半最大的禁止的集中 IC50 是在在 PAB 治疗以后的 36 和 48 h 的 3.4 和 1.35 亩 mol/L ,暴露于 PAB 的 respectively.The MCF-7 房间显示出 apoptosis 的典型特征,包括词法变化和 DNA 破碎。为 36 h 与 4 亩 mol/L PAB 对待的 MCF-7 房间经历了 apoptosis,然而并非坏死。PAB 导致的 apoptosis 独立于死亡受体小径。衰老的房间变得更大并且恭维,并且染色的 SA-p-galactosi-dase 是导致的 positive.PAB 明显的有丝分裂的拘捕和它先于 apoptosis 和老朽。p21 和 p53 的表情起来与 PAB 处理调整了,并且 cyclin B1 起来从细胞质调整了并且搬运,并且支撑了稳定的 levels.Conclusion 到原子核: PAB 在 MCF-7 房间导致了有丝分裂的拘捕并且通过 apoptosis 和老朽禁止了增长。apoptosis 独立于死亡受体小径。 | Jing-hua YU Qiao CUI Yuan-yuan JIANG Wei YANG Shin-ichi TASHIRO Satoshi ONODERA Takashi IKEJIMA | 2007 | Acta Pharmacologica Sinica2007，28，12： | 22 |
| 5 | Reactive oxygen species contribute to oridonininduced apoptosis and autophagy in human cervical carcinoma HeLa cells显示文摘瞄准: 在 HeLa 细胞在导致 oridonin 的 apoptosis 和 autophagy 调查反应的氧种类(ROS ) 的角色。 | Ya-hong ZHANG Ying-liang WU Shin-ichi TASHIRO Satoshi ONODERA Takashi IKEJIMA | 2011 | Acta Pharmacologica Sinica2011，32，10： | 18 |
| 6 | 为胃的癌症的内视镜的 submucosal 解剖的支持的技术和设备显示文摘 The indications for endoscopic treatment have expanded in recent years,and relatively intestinal-type mucosal stomach carcinomas with a low potential for metastasis are now often resected en bloc by endoscopic submucosal dissection(ESD),even if they measure over 20 mm in size.However,ESD requires complex maneuvers,which entails a long operation time,and is often accompanied by complications such as bleeding and perforation.Many technical developments have been implemented to overcome these complications.The scope,cutting device,hemostasis device,and other supportive devices have been improved.However,even with these innovations,ESD remains a potentially complex procedure.One of the major difficulties is poor visualization of the submucosal layer resulting from the poor countertraction afforded during submucosal dissection.Recently,countertraction devices have been developed.In this paper,we introduce countertraction techniques and devices mainly for gastric cancer. | Nobuyuki Sakurazawa Shunji Kato Itsuo Fujita Yoshikazu Kanazawa Hiroyuki Onodera Eiji Uchida | 2012 | World Journal of Gastrointestinal Endoscopy2012，4，6： | 18 |
| 7 | Non-invasive determination of hepatic steatosis by acoustic structure quantification from ultrasound echo amplitude显示文摘AIM:To use leptin-deficient(ob/ob) mice with demonstrated differences in steatosis levels to test a new diagnostic method using the acoustical structure quantification(ASQ) mode and the associated analytical parameter,'focal disturbance ratio'(FD-ratio).METHODS:Nine ob/ob mice,at 5,8,and 12 wk of age(n = 3 in each age group),were used as models for hepatic steatosis.Echo signals obtained from ultrasonography in the mice were analyzed by ASQ,which uses a statistical analysis of echo amplitude to estimate inhomogeneity in the diagnostic region.FD-ratio,as calculated from this analysis,was the focus of the present study.FD-ratio and fat droplet areas and sizes were compared between age groups.RESULTS:No fibrosis or inflammation was observed in any of the groups.The fat droplet area significantly(P < 0.01) increased with age from 1.25% ± 0.28% at 5 wk to 31.07% ± 0.48% at 8 wk to 51.69% ± 3.19% at 12 wk.The median fat droplet size also significantly(P < 0.01) increased with age,from 1.33(0.55-10.52) m at 5 wk,2.82(0.61-44.13) m at 8 wk and 6.34(0.66-81.83) m at 12 wk.The mean FD-ratio was 0.42 ± 0.11 at 5 wk,0.11 ± 0.05 at 8 wk,and 0.03 ± 0.02 at 12 wk.The FD-ratio was significantly lower at 12 wk than at 5 wk and 8 wk(P < 0.01).A significant negative correlation was observed between the FD-ratio and either the fat droplet area(r =-0.7211,P = 0.0017) or fat droplet size(r =-0.9811,P = 0.0052).CONCLUSION:This tool for statistical analysis of signals from ultrasonography using the FD-ratio can be used to accurately quantify fat in vivo in an animal model of hepatic steatosis,and may serve as a quantitative biomarker of hepatic steatosis. | Hidekatsu Kuroda Keisuke Kakisaka Naohisa Kamiyama Takayoshi Oikawa Mio Onodera Kei Sawara Kanta Oikawa Ryujin Endo Yasuhiro Takikawa Kazuyuki Suzuki | 2012 | World Journal of Gastroenterology2012，18，29： | 15 |
| 8 | Protein tyrosine kinase, JNK, and ERK involvement in p seudolaric acid B- induced apoptosis of human breast cancer MCF-7 cells显示文摘瞄准:在人的乳癌 MCF-7 房间调查 pseudolaric 酸 B (PAB ) 的 apoptotic 机制。方法:3-(4,5-Dimethylthiazol-2-yl )-2, 5-di-phenyltetrazolium 溴化物分析和词法变化被使用检测 apoptosis。apoptotic 和坏死的房间的百分比被 lactate 脱氢酶计算基于活动的 cytotoxicity 试金,和蛋白质表示被西方的污点分析检验。结果:包括 p38, c-Jun-N-terminal kinase (JNK ) 和细胞外的调整信号的 kinase (英皇家空军之阶级最低之兵) , PAB 或激活 mitogen 的蛋白质家族 ases 没参予坏死。P38 没为 36 h 在 4 μmol/L PAB 治疗以后在 apoptosis 上有明显的函数,但是 PAB 导致了 JNK phosphorylation 并且在 apoptotic 进程禁止了英皇家空军之阶级最低之兵 phosphorylation。在这学习蛋白质酷氨酸 kinase (PTK ) 的禁止者 genistein 转换了 PAB 的禁止的效果,相反支持 MCF-7 房间的幸存。象 genistein 一样,另一 PTK 禁止者 AG1024 在对待 PAB 的 MCF-7 房间上有类似的效果,显示 PAB 激活 PTK 导致 apoptosis。和 PAB, genistein 增加了津贴的表示,并且减少在在 36 h 的对待 PAB 的 MCF-7 房间的 JNK 和 p-JNK 的表情。并且津贴和 p-JNK 分别地是英皇家空军之阶级最低之兵和 JNK 的活跃模式,这被考虑。结论:PTK 英皇家空军之阶级最低之兵和 JNK 是在上游的,并且 PTK 通过激活 JNK 并且在对待 PAB 的 MCF-7 房间使英皇家空军之阶级最低之兵失去活性导致了 apoptosis。 | Jing-hua YU Hong-jun WANG Xiang-ru LI Shin-ichi TASHIRO Satoshi ONODERA Takashi IKEJIMA | 2008 | Acta Pharmacologica Sinica2008，29，9： | 12 |
| 9 | Crosstalk of ROS/RNS and autophagy in silibinin- induced apoptosis of MCF-7 human breast cancer cells in vitro显示文摘反应的氧种类(ROS ) 和反应的氮种类(RNS ) 在调整细胞幸存和死亡起重要作用。Silibinin 是与反肿瘤活动从牛奶蓟孤立的自然 polyphenolic flavonoid,但是在人的乳癌 MCF-7 房间导致 cytoprotective ROS/RNS 被发现。而且,有 silibinin 下面调整的治疗嗯在 MCF-7 房间的表示,并且导致 autophagy 和 apoptosis。在这研究,我们在 MCF-7 房间探索了在嗯联系小径和 RNS/ROS 之间的关系。我们也调查了在在对待 silibinin 的 MCF-7 房间表明小径的死亡位于在 ROS/RNS 层次和 autophagy 之间的相互的规定下面的分子的机制。Silibinin (100-300 mol/L ) dose-dependently 在 MCF-7 房间增加了 ROS/RNS 产生(与高表示嗯并且低表示嗯) 并且 MDA-MB-231 房间(与低表示嗯并且高表示嗯) 。清除 ROS/RNS 显著地提高了 MCF-7 房间,然而并非 MDA-MB231 房间的导致 silibinin 的死亡。药理学激活或封锁嗯分别地,在 MCF-7,房间显著地提高了或减少导致 silibinin 的 ROS/RNS 产生,而激活或块嗯没有效果。在对待 silibinin 的 MCF-7 房间,到 ROS/RNS 捐赠者的暴露减少了 autophagic 铺平,而有 3 麻省的 autophagy 的抑制显著地增加了 ROS/RNS 层次。我们进一步证明那在 ROS/RNS 产生增加,嗯激活或 autophagy 下面规定在对待 silibinin 的 MCF-7 房间有保护的角色。在一个条件下面嗯激活,清除 ROS/RNS 或刺激 autophagy 提高了 silibinin 的 cytotoxicity。这些结果表明二的存在在 MCF-7 房间的导致 silibinin 的死亡冲突小径:一个人包含下面规定嗯并且从而下游地扩充 pro-apoptotic autophagy,导致房间死亡;其它包含支持幸存的 ROS/RNS 的起来规定;并且 ROS/RNS 和 autophagy 形式的产生平衡的一个否定反馈环被调整由嗯。 | Nan ZHENG Lu LIU Wei-wei LIU Fei LI Toshihiko HAYASHI Shin-ichi TASHIRO Satoshi ONODERA Takashi IKEJIMA | 2017 | Acta Pharmacologica Sinica2017，38，2： | 11 |
| 10 | Pegylated interferon plus ribavirin for genotype Ib chronic hepatitis C in Japan显示文摘AIM:To evaluate the efficacy of pegylated interferon α-2b(peg-IFNα-2b) plus ribavirin(RBV) therapy in Japanese patients with chronic hepatitis C(CHC) genotype Ib and a high viral load.METHODS:One hundred and twenty CHC patients(58.3% male) who received peg-IFNα-2b plus RBV therapy for 48 wk were enrolled.Sustained virological response(SVR) and clinical parameters were evaluated.RESULTS:One hundred(83.3%) of 120 patients completed 48 wk of treatment.53 patients(44.3%) achieved SVR.Early virological response(EVR) and end of treatment response(ETR) rates were 50% and 73.3%,respectively.The clinical parameters(SVR vs non-SVR) associated with SVR,ALT(108.4 IU/L vs 74.5 IU/L,P = 0.063),EVR(76.4% vs 16.4%,P < 0.0001),adherence to peg-IFN(≥ 80% of planned dose) at week 12(48.1% vs 13.6%,P = 0.00036),adherence to peg-IFN at week 48(54.7% vs 16.2%,P < 0.0001) and adherence to RBV at week 48(56.1% vs 32.1%,P = 0.0102) were determined using univariate analysis,and EVR and adherence to peg-IFN at week 48 were determined using multivariate analysis.In the older patient group(> 56 years),SVR in females was significantly lower than that in males(17% vs 50%,P = 0.0262).EVR and adherence to Peg-IFN were demonstrated to be the main factors associated with SVR.CONCLUSION:Peg-IFNα-2b plus RBV combination therapy demonstrated good tolerability in Japanese patients with CHC and resulted in a SVR rate of 44.3%.Treatment of elderly female patients is still challenging and maintenance of adherence to peg-IFNα-2b is important in improving the SVR rate. | Takayuki Kogure Yoshiyuki Ueno Koji Fukushima Futoshi Nagasaki Yasuteru Kondo Jun Inoue Yasunori Matsuda Eiji Kakazu Takeshi Yamamoto Hiroyoshi Onodera Yutaka Miyazaki Hiromasa Okamoto Takehiro Akahane Tomoo Kobayashi Yutaka Mano Takao Iwasaki Motoyasu Ishii Tooru Shimosegawa | 2008 | World Journal of Gastroenterology2008，14，47： | 7 |
| 11 | Oridonin induces apoptosis and autophagy in murine fibrosarcoma L929 cells partly via NO-ERK-p53 positive-feedback loop signaling pathway显示文摘瞄准: 调查氮的氧化物的角色(没有) 在在鼠科的纤维肉瘤 L929 房间和内在的分子的机制的导致 oridonin 的 apoptosis 和 autophagy。 | Yuan-chao YE Hong-ju WANG Lei XU Wei-wei LIU Bin-bin LIU Shin-lchi TASHIRO Satoshi ONODERA Takashi IKEJIMA | 2012 | Acta Pharmacologica Sinica2012，33，8： | 6 |
| 12 | 1100kV气体绝缘双断口断路器的开发与产品化(英文)显示文摘开发了一种用于气体绝缘组合电器(GIS)的1100kV50kASF6气体双断口断路器(GCB)。研究了这种断路器的各种特性,并成功完成了在绝缘、开断和机械性能等方面的型式试验。实现了GCB的小型化和轻量化。这种新开发的GCB已应用在中国特高压交流示范工程的GIS中,并已于2009年年初投入使用。 | Rei Hemmi Michiru Onodera Yoshikatsu Enokida Naoyuki Yamamoto Kunio Hirasawa | 2009 | 电网技术2009，33，15： | 5 |
| 13 | Activation of p53 contributes to pseudolaric acid B-induced senescence in human lung cancer cells in vitro显示文摘瞄准:Pseudolaric 酸 B (PAB ) ,从根孤立的 diterpene 酸金钱松属 kaempferi Gordon 吠叫,出现了经由导致 apoptosis 在几根癌症房间线跟随的房间周期拘捕施加反肿瘤效果。这里,我们报导 PAB 在人的肺癌症 A549 房间导致了一场有丝分裂的大祸,没有 apoptosis 或 necrosis.Methods,它导致了老朽:三根人的肺癌症房间线(A549, H460 和 H1299 房间) 被检验。细胞生长抑制与 MTT 试金被估计。房间周期分发用流动 cytometer 被决定。房间原子形态学在一台荧光显微镜下面被观察。衰老的房间用 SA-β 被检测;染色的女郎。Apoptotic 和衰老的蛋白质表示用西方的污点分析被检验。在房间的 p53 和 p21 的表示是由 siRNAs.Results 的 downregulated:有 PAB 的治疗(5-80 μ mol/L ) 在剂量依赖者和时间依赖者礼貌禁止了 A549 房间的生长。有 PAB 的延长治疗(20 μ mol/L ) 在白天 1 点的引起的 G 2/M 拘捕从白天 2 由有丝分裂的大祸列在后面,它最后导致了在天之间的房间老朽 3 和 4 没有房间死亡(apoptosis 或坏死) 。有 siRNA 的 p53 表示击倒在 A549 房间的显著地压制的导致 PAB 的老朽(p53 野) 。而且,导致 PAB 的老朽也在人的肺癌症 H460 房间被观察(p53 野) ,然而并非在人的肺癌症 H1299 房间(p53 0 ).Conclusion:对在 vitro 的人的肺癌症 A549 房间的 PAB 的反肿瘤行动通过 p53 小径的激活包含老朽的正式就职。 | Guo-dong YAO Jing YANG Qiang LI Ye ZHANG Min QI Si-miao FAN Toshihiko HAYASHI Shin-ichi TASHI RO Satoshi ONODERA Takashi IKEJIMA | 2016 | Acta Pharmacologica Sinica2016，37，7： | 5 |
| 14 | Involvement of mitochondria and caspase pathways in N-demethyl-clarithromycin-induced apoptosis in human cervical cancer HeLa cell显示文摘瞄准:学习 N-demethyl-clarithromycin (NDC ) 在 vitro 由导致人的颈的癌症 HeLa 房间 apoptosis 的机制。方法:生存能力 ofN-demethyl-clarithromycin-induced HeLa 房间被 MTT 试金测量。有压缩原子核的 Apoptotic 房间被阶段对比显微镜学设想。Nucleosomal DNA 破碎是由统帅玫瑰胶化电气泳动的 assayed。mitochondrial transmembrane 潜力的测量被 FACScan flowcytometer 分析。Caspase-3, poly-( 自动数据处理核糖) 聚合酶(PARP ) ,激活 caspase 的 DNase (ICAD ) , Bcl-2, Bax, p53,和 SIRT1 蛋白质表示和细胞色素 c 的版本被西方的污点分析检测。结果:N-demethyl-clarithromycin, ananti 煽动性的物质,在一个剂量依赖者的禁止的 HeLa 房间生长和时间依赖者 manner.N-demethyl-clarithro-mycin 通过 apoptotic 小径导致了 HeLa 房间死亡。pan-caspaseinhibitor (z-VAD-fmk ) , caspase-3 禁止者(z-DEVD-fmk ) 和 caspase-9 禁止者(z-LEHD-fmk ) 部分提高了 N-demethyl-clarithromycin 导致的房间生存能力,但是 caspase-8 禁止者(z-IETD-fmk ) 没几乎有效果。Caspase-3 被激活然后由降级 ofcaspase-3 底层列在后面, ICAD 和 PARP 的禁止者。同时, mitochondrial transmembranepotential 显著地被减少,在 cytosol 的细胞色素 c 的版本是 increased.N-demethyl-clarithromycin 在上面调整了 mitochondrial Bax/Bcl-2 的表示比率,并且显著地增加了 p53 蛋白质的表示。它也击倒调整 anti-apoptoticprotein SIRT1 表示。结论:N-demethyl-clarithromycin 经由 mitochondrial 小径在 HeLa 房间导致了 apoptosis。 | Ai-min QIAO Takashi IKEJIMA Shin-ichi TASHIRO Satoshi ONODERA Wei-ge ZHANG Ying-liang WU | 2006 | Acta Pharmacologica Sinica2006，27，12： | 5 |
| 15 | Blocking the utilization of glucose induces the switch from senescence to apoptosis in pseudolaric acid B-treated human lung cancer cells in vitro显示文摘Pseudolaric 酸 B (PAB ) ,从根孤立的 diterpene 酸金钱松属 kaempferi Gordon 吠叫,在几根癌症房间线施加反肿瘤效果。我们的以前的学习证明 PAB 主要在人的肺癌症 A549 细胞的生长的抑制经由 p53-p21 激活而非 apoptosis 导致了老朽(p53 野类型) 。在 p53 空的人的肺癌症 H1299 房间,然而,没有老朽, PAB 引起了 apoptosis。在这研究,我们调查了什么机制为从老朽的开关负责到在对待 PAB 的人的肺癌症房间线的 apoptosis。衰老的房间被 SA 检验 -- 染色的女郎。葡萄糖举起和 apoptosis 比率用 FACScan 流动 cytometer 被估计。商业试金工具包被用来测量 ATP 的层次并且喂奶。siRNA 的 Transfection 习惯于击倒 p53 或 p21 的表示。西方的污点分析被使用测量蛋白质表示层次。在 p53 野类型的 A549 房间, PAB (20 mol/L ) 引起了老朽,和 time-dependently 增加的葡萄糖利用;p53 或 p21 击倒显著地减少了葡萄糖的举起和新陈代谢但是提高的导致 PAB 的 apoptosis。用 glycolytic 禁止者 2-DG (1 mmol/L ) 的葡萄糖利用的抑制显著地提高了 apoptosis 正式就职。类似的结果在野类型的 H460 房间与 PAB 对待的另一 p53 被观察。在 PAB time-dependently 减少了的地方,相反的结果在 p53 空的 H1299 房间被发现葡萄糖利用,并且导致了仅仅 apoptosis。我们的结果证明导致 PAB 的老朽与提高的葡萄糖利用被联系,并且降低葡萄糖利用可能贡献 apoptosis 感应。因此,堵住葡萄糖利用从老朽贡献开关到 apoptosis,并且 p53 在这个过程起一个重要作用。 | Guo-dong YAO Jing YANG Xiu-xiu LI Xiao-yu SONG Toshihiko HAYASHI Shin-ichi TASHIRO Satoshi ONODERA Shao-jiang SONG Takashi IKEJIMA | 2017 | Acta Pharmacologica Sinica2017，38，10： | 4 |
| 16 | Feasibility of drug delivery to the eye’s posterior segment by topical instillation of PLGA nanoparticles显示文摘We investigated the delivery of drugs to the posterior segment of the eye by non-invasive topical instillation using submicron-sized poly(D,L-lactide-co-glycolide)(PLGA) nanoparticles(NPs). Surface-modified PLGA NPs were developed to improve the drug delivery efficiency to the retina and were administered as topical eye drops to mice. Chitosan(CS) and glycol chitosan(GCS), which are mucoadhesive polymers, and polysorbate 80(P80) were used as surface modifiers, and have been reported to increase the association of NPs with cells.Coumarin-6 was used as a model drug and fluorescent marker, and after ocular administration of PLGA NP eye drops, the fluorescence intensity of coumarin-6 was observed in the retina. The fluorescence image analysis indicated that there are several possible routes to the retina and fates of PLGA NPs in ocular tissue, and that these pathways involved the corneal,non-corneal, or uveal routes. Delivery to the mouse retina segments after topical administration was increased by surface modification with CS, GCS, or P80. Surface-modified PLGA NPs are a promising method for retinal drug delivery via topical instillation. | Kohei Tahara Keiichi Karasawa Risako Onodera Hirofumi Takeuchi | 2017 | Asian Journal of Pharmaceutical Sciences2017，12，4： | 3 |
| 17 | THE EFFECTS OF RADIX SALVIAE MILTIORRHIZAE ON LIPID ACCUMULATION OF PEROXIDIZED LOW DENSITY LIPOPROTEIN IN MOUSE PERITONEAL MACROPHAGES ?LIPID ANALYSIS AND MORPHOLOGICAL STUDIES显示文摘Mouse peritoneal macrophages were incubated in DMEM with pox-LDL and Rradlx Salviae Miltiorrhizae (RSM) to investigate the effects of RSM on the internalization of peroxidized low density lipoprotein (pox-LDL) by using lipid analysis and electron microscopy. Lipid peroxide (LPO) concentrations were increased slightly in the medium after incubation of macrophages with normal LDL (n-LDL), while decreased significantly in the media after incubation of macrophages with pox-LDL. In the three groups with pox-LDL, it could be found that there was a dose-dependent decrease of concentrations of LPO and total cholesterol (TCH) in the two RSM groups, and the decrease in the two RSM groups was much greater than in the group without RSM. RSM accelerated a more decrease of LPO than cholesterol contents in the media containing pox-LDL. The ultrastructural studies also showed that RSM induced the accumulation of lipid droplets in the cytoplasm of mouse peritoneal macrophages. The results suggested that RSM could accelerate the phagocytosis and degradation of pox-LDL by macrophages. | 于生元 匡培根 Takemichi Kanazawa Kogo Onodera Hirohumi Metoki Yasaburo Oike | 1998 | Journal of Traditional Chinese Medicine1998，18，4： | 2 |
| 18 | Influence of the safety and diagnostic accuracy of preoperative endoscopic ultrasound-guided fine-needle aspiration for resectable pancreatic cancer on clinical performance显示文摘AIM:To evaluate the safety and diagnostic accuracy of endoscopic ultrasound-guided fine-needle aspiration(EUS-FNA)in a cohort of pancreatic cancer patients.METHODS:Of 213 patients with pancreatic cancer evaluated between April 2007 and August 2011,82were thought to have resectable pancreatic cancer on the basis of cross-sectional imaging findings.Of these,54 underwent EUS-FNA before surgery(FNA+group)and 28 underwent surgery without preoperative EUSFNA(FNA-group).RESULTS:All 54 lesions were visible on EUS,and all54 attempts at FNA were technically successful.The diagnostic accuracy according to cytology and histology findings was 98.1%(53/54)and 77.8%(42/54),respectively,and the total accuracy was 98.1%(53/54).One patient developed mild pancreatitis after EUS-FNA but was successfully treated by conservative therapy.No severe complications occurred after EUS-FNA.In the FNA+and FNA-groups,the median relapse-free survival(RFS)was 742 and 265 d,respectively(P=0.0099),and the median overall survival(OS)was1042 and 557 d,respectively(P=0.0071).RFS and OS were therefore not inferior in the FNA+group.These data indicate that the use of EUS-FNA did not influence RFS or OS,nor did it increase the risk of peritoneal recurrence.CONCLUSION:In patients with resectable pancreatic cancer,preoperative EUS-FNA is a safe and accurate diagnostic method. | Taiki Kudo Hiroshi Kawakami Masaki Kuwatani Kazunori Eto Shuhei Kawahata Yoko Abe Manabu Onodera Nobuyuki Ehira Hiroaki Yamato Shin Haba Kazumichi Kawakubo Naoya Sakamoto | 2014 | World Journal of Gastroenterology2014，20，13： | 2 |
| 19 | Heparin bridge therapy and post-polypectomy bleeding显示文摘AIM To identify risk factors for post-polypectomy bleeding(PPB), focusing on antithrombotic agents. METHODS This was a case-control study based on medical records at a single center. PPB was defined as bleeding that occurred 6 h to 10 d after colonoscopic polypectomy and required endoscopic hemostasis. As risk factors for PPB, patient-related factors including anticoagulants, antiplatelets and heparin bridge therapy as well as polyp- and procedure-related factors were evaluated. All colonoscopic hot polypectomies, endoscopic mucosal resections and endoscopic submucosal dissections performed between January 2011 and December 2014 were reviewed. RESULTS PPB occurred in 29(3.7%) of 788 polypectomies performed during the study period. Antiplatelet or anticoagulant agents were prescribed for 210(26.6%)patients and were ceased before polypectomy except for aspirin and cilostazol in 19 cases. Bridging therapy using intravenous unfractionated heparin was adopted for 73 patients. The univariate analysis revealed that anticoagulants, heparin bridge, and anticoagulants plus heparin bridge were significantly associated with PPB(P < 0.0001) whereas antiplatelets and antiplatelets plus heparin were not. None of the other factors including age, gender, location, size, shape, number of resected polyps, prophylactic clipping and resection method were correlated with PPB. The multivariate analysis demonstrated that anticoagulants and anticoagulants plus heparin bridge therapy were significant risk factors for PPB(P < 0.0001). Of the 29 PPB cases, 4 required transfusions and none required surgery. A thromboembolic event occurred in a patient who took anticoagulant. CONCLUSION Patients taking anticoagulants have an increased risk of PPB, even if the anticoagulants are interrupted before polypectomy. Heparin-bridge therapy might be responsible for the increased PPB in patients taking anticoagulants. | Toshiyuki Kubo Kentaro Yamashita Kei Onodera Tomoya Iida Yoshiaki Arimura Masanori Nojima Hiroshi Nakase | 2016 | World Journal of Gastroenterology2016，22，45： | 2 |
| 20 | Combined thoracoscopic and endoscopic surgery for a large esophageal schwannoma显示文摘A 47-year-old woman presented to our hospital with complaints of dysphagia. Esophagogastroduodenoscopy identified a submucosal tumor in the left wall of the esophagus that was diagnosed as a benign schwannoma on biopsy. Computed tomography revealed a tumor of length 60 mm in the thoracic esophagus, with its cranial edge at the level of the aortic arch. On endoscopy, a submucosal tunnel was created 40 mm proximal to the cranial edge of the tumor, and its oral end was dissected from the mucosal and muscular layers. This was followed by the resection of the entire tumor by left-sided thoracoscopy. The esophageal defect was closed in layer by continuous suture from the thoracic side. Endoscopic closure was achieved by using clips. No postoperative complications were observed. Oral diet was resumed from postoperative day 7 and the patient was discharged on postoperative day 9. This combined approach has not been described for similar tumors. Our experience demonstrated that large esophageal tumors can be safely excised with minimally invasive surgery by using a combination of thoracoscopy and endoscopy. | Yu Onodera Toru Nakano Daisuke Takeyama Shota Maruyama Yusuke Taniyama Tadashi Sakurai Takahiro Heishi Chiaki Sato Takuro Kumagai Takashi Kamei | 2017 | World Journal of Gastroenterology2017，23，46： | 2 |
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