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| 1 | Hierarchical Nanogold Labels to Improve the Sensitivity of Lateral Flow Immunoassay显示文摘Lateral flow immunoassay(LFIA) is a widely used express method and offers advantages such as a short analysis time, simplicity of testing and result evaluation.However, an LFIA based on gold nanospheres lacks the desired sensitivity, thereby limiting its wide applications.In this study, spherical nanogold labels along with new types of nanogold labels such as gold nanopopcorns and nanostars were prepared, characterized, and applied for LFIA of model protein antigen procalcitonin. It was found that the label with a structure close to spherical provided more uniform distribution of specific antibodies on its surface, indicative of its suitability for this type of analysis.LFIA using gold nanopopcorns as a label allowed procalcitonin detection over a linear range of 0.5–10 ng mL^(-1) with the limit of detection of 0.1 ng mL^(-1), which was fivefold higher than the sensitivity of the assay with gold nanospheres. Another approach to improve the sensitivity of the assay included the silver enhancement method,which was used to compare the amplification of LFIA for procalcitonin detection. The sensitivity of procalcitonin determination by this method was 10 times better the sensitivity of the conventional LFIA with gold nanosphere as a label. The proposed approach of LFIA based on gold nanopopcorns improved the detection sensitivity without additional steps and prevented the increased consumption of specific reagents(antibodies). | Kseniya Serebrennikova Jeanne Samsonova Alexander Osipov | 2018 | Nano-Micro Letters2018，10，2： | 5 |
| 2 | Inhibition of focal adhesion kinase enhances antitumor response of radiation therapy in pancreatic cancer through CD8+ T cells显示文摘Objective:Pancreatic ductal adenocarcinoma(PDAC)is a deadly malignancy,due in large part to its resistance to conventional therapies,including radiotherapy(RT).Despite RT exerting a modest antitumor response,it has also been shown to promote an immunosuppressive tumor microenvironment.Previous studies demonstrated that focal adhesion kinase inhibitors(FAKi)in clinical development inhibit the infiltration of suppressive myeloid cells and T regulatory(T regs)cells,and subsequently enhance effector T cell infiltration.FAK inhibitors in clinical development have not been investigated in combination with RT in preclinical murine models or clinical studies.Thus,we investigated the impact of FAK inhibition on RT,its potential as an RT sensitizer and immunomodulator in a murine model of PDAC.Methods:We used a syngeneic orthotopic murine model to study the effect of FAKi on hypofractionated RT.Results:In this study we showed that IN10018,a small molecular FAKi,enhanced antitumor response to RT.Antitumor activity of the combination of FAKi and RT is T cell dependent.FAKi in combination with RT enhanced CD8+T cell infiltration significantly in comparison to the radiation or FAKi treatment alone(P<0.05).FAKi in combination with radiation inhibited the infiltration of granulocytes but enhanced the infiltration of macrophages and T regs in comparison with the radiation or FAKi treatment alone(P<0.01).Conclusions:These results support the clinical development of FAKi as a radiosensitizer for PDAC and combining FAKi with RT to prime the tumor microenvironment of PDAC for immunotherapy. | Arsen Osipov Alex B.Blair Juliane Liberto Jianxin Wang Keyu Li Brian Herbst Yao Xu Shiqi Li Nan Niu Rufiaat Rashid Ding Ding Yanan Liu Zaiqi Wang Christopher L.Wolfgang Richard A.Burkhart Daniel Laheru Lei Zheng | 2021 | Cancer Biology & Medicine2021，18，1： | 3 |
| 3 | The classification of microstructures of clay soils 显示文摘 | Sergeyev Y M Grabowska-Olszewska B Osipov V I | 1980 | Journal of Microscopy1980，120，12： | 2 |
| 4 | The synchronization of chaotic systems显示文摘 | Boccaletti S Kurths J Osipov G | 2002 | Phys Rep2002，366，： | 1 |
| 5 | The synchronization of chaotic systems显示文摘 | Boccaletti S Kurths J Osipov G | 2002 | Physics Report2002，366，： | 1 |
| 6 | The synchronization of chaotic systems显示文摘 | Boccaletti S Kurths J Osipov G | 2002 | Physics Reports2002，366，： | 1 |
| 7 | The conundrum of traumatic colon injury显示文摘 | Fealk M Osipov R Foster K | 2004 | Am J Surg2004，188，6： | 1 |
| 8 | Infrared spectra and structure of salts of nitroimidzoles 显示文摘 | Epishina L V Slovetskii V I Osipov V G | 1967 | Khim Geterotsikl Soedin1967，，4： | 1 |
| 9 | Investigation of microbial diversity of underground waters in monitoring deep horizons of the Earth's crust显示文摘 | KONDAKOVA G V VERKHOVTSEVA N V OSIPOV G A | 2007 | Moscow University Biological Sciences Bulletin2007，62，2： | 1 |
| 10 | Intelligent system for fish stock prediction and allowable catch evaluation显示文摘 | Sazonova L Osipov G Godovnikov M | 1999 | Environment Modeling &Software1999，，14： | 1 |
| 11 | Microwave properties of composites with glass coated amorphous magnetic microwires显示文摘 | STAROSTENKO S N ROZANOV K N OSIPOV A V | 2006 | J Magn Magn Mater2006，298，： | 1 |
| 12 | Relation of left atrial size to function as determined by transesophageal echocardiography显示文摘 | Blondheim DS Osipov A Meisel SR | 2005 | Am J Cardiol2005，96，： | 1 |
| 13 | Fabrication of Nd3+ : YAG laser ceramics with various ap- proaches 显示文摘 | BAGAYEV S N OSIPOV V V SOLOMONOV V I | 2012 | Opt Mater2012，34，8： | 1 |
| 14 | The early diagnostic of chorionic-amnionitic and intrauterine infection on content of nitrite and non-tiolate nitrosocompounds in plasma under premature discharge of amniotic fluid显示文摘 | Titov VIu Osipov AN Balitskii SP | 2013 | Klin Lab Diagn2013，12，： | 1 |
| 15 | The synchronization of chaotic systems 显示文摘 | Kurths J Osipov G | 2002 | Physics Report2002，366，： | 1 |
| 16 | A concise enantioselective synthesis of (-)-ranirestat 显示文摘 | Trost B M Osipov M Dong G B | 2010 | Org Lett2010，12，6： | 1 |
| 17 | Antibiotic-associated diarrhea显示文摘 | Parfenov A I Ruchkina I N Osipov G A | 2006 | JMissStateMedAssoc2006，44，11： | 1 |
| 18 | 'Steal'is an unestablished mechanism for the clinical presentation of cerebral arteriovenous malformations显示文摘 | Mast H Mohr JP Osipov A | 1995 | Stroke1995，26，7： | 1 |
| 19 | Ubiquinone-dependent recycling of vitamin E radicals by superoxide 显示文摘 | Stoyanovsky DA Osipov AN Quinn P J | 1995 | Arch Bioehem Bio- phys1995，323，： | 1 |
| 20 | Effect of hydrogen sulfide on myocardial protection in the setting of cardioplegia and cardiopulmo- nary bypass显示文摘 | Osipov RM Robich MP Feng J | 2010 | Interact Cardivovasc Tborac Surg2010，10，： | 1 |
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