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| 1 | Evaluation of the AGCU Expressmarker 16 and 22 PCR Amplification Kits Using Biological Samples Applied to FTA Micro Cards in Reduced Volume Direct PCR Amplification Reactions显示文摘This study evaluated the performance of the Wuxi AGCU ScienTech Incorporation(HuiShan,Wuxi,China)AGCU Expressmarker 16(EX 16)and 22(EX22)short tandem repeat(STR)amplification kits in reduced reaction volumes using direct polymerase chain reaction(PCR)amplification workflows.The commercially available PowerPlex21(PP21)System(Promega,Wisconsin,USA),which follows similar direct workflows,was used as a reference.Anticoagulate blood applied to chemically impregnated FTATM Micro Cards(GE Healthcare UK Limited,Amersham Place,Little Chalfont,Buckinghamshire,HP79NA,UK)was used to represent a complex biological sample.Allelic concordance,first‑pass success rate,average peak heights,heterozygous peak height ratios(HPHRs),and intracolor and intercolor peak height balance were determined.In reduced volume PCR reactions,the performances of both the EX16 and EX22 STR amplification kits were comparable to that of the PP21 System.The level of performance was maintained at PCR reaction volumes,which are 40%of that recommended.The EX22 and PP21 System kits possess comparable overlapping genome coverage.This study evaluated the performance of the AGCU EX16 and EX22 STR amplification kits in reduced PCR reaction volumes using direct workflows in combination with whole blood applied to FTATM Micro Cards.Allelic concordance,first‑pass success rate,average peak heights,HPHRs,and intracolor and intercolor peak height balance were determined.A concordance analysis was completed that compared the performance of the EX16 and EX22 kits using human blood applied to FTA Micro Cards in combination with full,half,and reduced PCR reaction volumes.The PP21 System(Promega)was used as a reference kit.Where appropriate,the distributions of data were assessed using the Shapiro‑Wilk test.For normally‑distributed data,statistics were calculated using analysis of variance(ANOVA)and for nonparametric data the Wilcoxon/Kruskal‑Wallis test was used.Statistical significance was set at P<0.05.Confidence intervals for mean values were set at 95%.On using reduced volume PCR reactions in combination with dried blood spots applied to FTA sample collection cards,both the EX16 and EX22 kits were shown to generate STR profiles of sufficient quality to allow entry into National DNA databases.The performance of both EX16 and EX22 was comparable to that of the PP21 System.This study demonstrates the successful use of the Wuxi AGCU ScienTech Incorporation EX16 and EX22 kits in reduced PCR reaction volumes with complex biological samples applied to chemically impregnated FTA sample collection cards. | Samantha J Ogden Kathryn L Lamerton Peter J Tatnell | 2015 | Journal of Forensic Science and Medicine2015，1，1： | 2 |
| 2 | An exploratory cross-country comparison of strategic purchasing 显示文摘 | Jeffrey A Ogden Christian L Rossetti | 2007 | Journal of Purchasing and Supply Management2007，13，1： | 1 |
| 3 | On the calibration and verification of two-dimensional, distributed, hortonian, continuous watershed models显示文摘 | SENARATH S U S OGDEN F L DOWNER C W | 2000 | Water Resources Research2000，36，： | 1 |
| 4 | Remov efficiency and bind- ing mechanisms of copper and copper-EDTA complexes using po-lyethyleneimine显示文摘 | Maketon W Zenner C S Ogden K L | 2008 | Environmental Science and Technology2008，42，6： | 1 |
| 5 | Parenteral glutamine increases serum heat shock protein 70 in critically ill patients显示文摘 | Ziegler T R Ogden L G Singleton K D | 2005 | Intensive Care Med2005，31，8： | 1 |
| 6 | Use of Blueberry to Study Genetic Control of Chilling Requirement and Cold Hardiness in Woody Perennials显示文摘 | Rowland L J Ogden E L Arora R | 1999 | Hort Sci1999，34，7： | 1 |
| 7 | Evaluation of Midwinter Cold Hardiness among 25 Rabbiteye blueberry c ultivar显示文摘 | Ehlenfeldt M K Ogden E L Rowland L J | 2006 | Hort Sci2006，41，3： | 1 |
| 8 | Enhanced lipid stability in whole wheat flour by lipase inactivation and antioxidant retention显示文摘 | ROSE D J OGDEN L V DUNN M L | 2008 | Cereal Chemistry2008，85，2： | 1 |
| 9 | A cosmetic 'anti ageing' product improves photoaged skin: a double blind, randomized con- trolled trial显示文摘 | WATSON S OGDEN L F | 2009 | British Journal of Dermatology2009，161，： | 1 |
| 10 | Prevalence of high body mass index in US children and Adolescents, 2007-2008显示文摘 | Ogden C L Carroll M D Curtin L R | 2010 | JAMA2010，，303： | 1 |
| 11 | Prevalence and trends in overweight among US children and adolescent,1999 - 2000显示文摘 | OGDEN C L FLEGAL K M CARRLL M D | 2002 | JAMA2002，288，： | 1 |
| 12 | Prevalence of overweight and obesity in the UnitedStates,1999-2004显示文摘 | OGDEN C L CARROLL M D CURTIN L R | 2006 | JAMA2006，295，： | 1 |
| 13 | Prediction of runoff and soil moistures at the watershed scale : Effects of model complexity and parameter assignment显示文摘 | Downer C W Ogden F L | 2003 | Water Resources Research2003，39，3： | 1 |
| 14 | Chlorine and other constituents in relation to tobacco leaf- burn显示文摘 | Myhre D L Attoe O J Ogden W B | 1956 | Soil Science Society of Ameri-ca Journal1956，20，4： | 1 |
| 15 | 2000 CDC Growth Charts for the United States: methods and development显示文摘 | Kuczmarski R J Ogden C L Guo S S | 2002 | Vital Health Stat 112002，，246： | 1 |
| 16 | Use of a cytokine in conjugate for efficient shoot regeneration from leaf sections of high bush blueberry显示文摘 | Rowland L J Ogden E L | 1992 | HortScience1992，27，10： | 1 |
| 17 | CDCgrowth charts:United States显示文摘 | Kuczmarski R J Ogden C L Grummer Strawn L M | 2000 | Adv Data2000，，314： | 1 |
| 18 | The role of beak shape in Octopodid taxonomy 显示文摘 | Ogden R S Allcock A L Watts P C | 1998 | South Africa Journal of marine Science1998，20，： | 1 |
| 19 | Data analytic wavelet threshold selection in 2-D signal denoising显示文摘 | HILTON L OGDEN T | 1997 | IEEE Transactions on Signal Processing1997，45，2： | 1 |
| 20 | Dietary fiber intake and reduced risk of coronary heart disease in US men and women:the National Health and Nutrition Examination Survey I Epidemiologic Follow-up Study显示文摘 | BAZZANO L A HE J OGDEN L G | 2003 | Arch Intern Med2003，163，16： | 1 |
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