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| 1 | Zika virus and neural developmental defects: building a case for a cause显示文摘Zika virus(ZIKV),a little-known flavivirus until a few months ago,is currently at the forefront of public health concerns worldwide because of its suspected role in causing microcephaly and other developmental defects in fetuses of infected mothers.On February 1,2016,the World Health Organization declared a Public Health Emergency of International Concern(PHEIC)for ZIKV.Discovered more | Sarah C Ogden Christy Hammack Hengli Tang | 2016 | Science China(Life Sciences)2016，59，5： | 2 |
| 2 | Evaluation of the AGCU Expressmarker 16 and 22 PCR Amplification Kits Using Biological Samples Applied to FTA Micro Cards in Reduced Volume Direct PCR Amplification Reactions显示文摘This study evaluated the performance of the Wuxi AGCU ScienTech Incorporation(HuiShan,Wuxi,China)AGCU Expressmarker 16(EX 16)and 22(EX22)short tandem repeat(STR)amplification kits in reduced reaction volumes using direct polymerase chain reaction(PCR)amplification workflows.The commercially available PowerPlex21(PP21)System(Promega,Wisconsin,USA),which follows similar direct workflows,was used as a reference.Anticoagulate blood applied to chemically impregnated FTATM Micro Cards(GE Healthcare UK Limited,Amersham Place,Little Chalfont,Buckinghamshire,HP79NA,UK)was used to represent a complex biological sample.Allelic concordance,first‑pass success rate,average peak heights,heterozygous peak height ratios(HPHRs),and intracolor and intercolor peak height balance were determined.In reduced volume PCR reactions,the performances of both the EX16 and EX22 STR amplification kits were comparable to that of the PP21 System.The level of performance was maintained at PCR reaction volumes,which are 40%of that recommended.The EX22 and PP21 System kits possess comparable overlapping genome coverage.This study evaluated the performance of the AGCU EX16 and EX22 STR amplification kits in reduced PCR reaction volumes using direct workflows in combination with whole blood applied to FTATM Micro Cards.Allelic concordance,first‑pass success rate,average peak heights,HPHRs,and intracolor and intercolor peak height balance were determined.A concordance analysis was completed that compared the performance of the EX16 and EX22 kits using human blood applied to FTA Micro Cards in combination with full,half,and reduced PCR reaction volumes.The PP21 System(Promega)was used as a reference kit.Where appropriate,the distributions of data were assessed using the Shapiro‑Wilk test.For normally‑distributed data,statistics were calculated using analysis of variance(ANOVA)and for nonparametric data the Wilcoxon/Kruskal‑Wallis test was used.Statistical significance was set at P<0.05.Confidence intervals for mean values were set at 95%.On using reduced volume PCR reactions in combination with dried blood spots applied to FTA sample collection cards,both the EX16 and EX22 kits were shown to generate STR profiles of sufficient quality to allow entry into National DNA databases.The performance of both EX16 and EX22 was comparable to that of the PP21 System.This study demonstrates the successful use of the Wuxi AGCU ScienTech Incorporation EX16 and EX22 kits in reduced PCR reaction volumes with complex biological samples applied to chemically impregnated FTA sample collection cards. | Samantha J Ogden Kathryn L Lamerton Peter J Tatnell | 2015 | Journal of Forensic Science and Medicine2015，1，1： | 2 |
| 3 | The usefulness of amplified fragment length polymorphism markers for taxon discrimination across graduated fine evolutionary levels in Caribbean Anolis Hzards 显示文摘 | Ogden R Thorpe R S | 2002 | Mol Eeol2002，，11： | 1 |
| 4 | An exploratory cross-country comparison of strategic purchasing 显示文摘 | Jeffrey A Ogden Christian L Rossetti | 2007 | Journal of Purchasing and Supply Management2007，13，1： | 1 |
| 5 | On the calibration and verification of two-dimensional, distributed, hortonian, continuous watershed models显示文摘 | SENARATH S U S OGDEN F L DOWNER C W | 2000 | Water Resources Research2000，36，： | 1 |
| 6 | A biomeehanieal study of simulated femoral neck fracture fixation by cannulated screws:effects of placement angle and number of screws显示文摘 | Walker E Mukherjee DP Ogden AI | 2007 | Am J Orthop2007，36，12： | 1 |
| 7 | Surgical strategies for treating patients with pineal region tumors 显示文摘 | Bruce Jn Ogden AT | 2004 | J Neurooncol2004，69，： | 1 |
| 8 | Shock wave therapy (orthotripsy) in musculoskeletal disorders显示文摘 | Ogden JA Alvarez RG Levitt R | 2001 | Clin Orthop2001，387，： | 1 |
| 9 | FK506 rescues peripheral nerve alIografts in acute rejection显示文摘 | Feng FY Ogden MA Myckatyn TM | 2001 | Nenrotrauma2001，18，2： | 1 |
| 10 | Prevalence of overweight and obesity among US children, adolescents, and adults, 1999-2002 显示文摘 | Hedley AA Ogden CL Johnson CL | 2004 | JAMA2004，291，23： | 1 |
| 11 | Prevalence of over- weight an d obesity in the United States, 1999 - 2004 显示文摘 | OGDEN CL CARROLL M D CU RT IN LR | 2006 | JAMA2006，29，13： | 1 |
| 12 | Large deformation isotropic elasticity:On the correlation of theory and experiment for incompressible rubber-like solids显示文摘 | Ogden R W | 1972 | Mathematical and Physical Sciences1972，326，56： | 1 |
| 13 | Prevalence and trends in obesity among US adults, 1999 - 2000显示文摘 | Carroll MD Ogden CL | 2002 | JAMA2002，288，： | 1 |
| 14 | Neurological,neuropsychological,and functional outcome following treatment for unruptured intracranial aneurysms显示文摘 | Ogden JA Mee E | 2005 | J Int Neuropsychol Soc2005，11，： | 1 |
| 15 | Principles of shock wave therapy显示文摘 | Ogden JA Toth-Kischkat A Sehuhheiss R | 2001 | ClinOrthop2001，387，： | 1 |
| 16 | Control of Listeria monocytogenes in salmon;antimicrobial effect of salting,smoking and specific smoke compounds显示文摘 | NIEDZIELA J MACRAE M OGDEN I | 1998 | Lebensmittel Wissenschaft Technologie1998，31，2： | 1 |
| 17 | Prevalence of overweight and obesity among US children, adolescents, and adults, 1999-2002显示文摘 | Hedley AA Ogden CL Johnson CL | 2004 | JAMA2004，291，23： | 1 |
| 18 | Recent Advances in the phenomenological Theory of Rubber Elasticity显示文摘 | Ogden R W | 1986 | Rubber Chemistry and Technology1986，，59： | 1 |
| 19 | Role of surfactant proteins A,D, and Clq in the clearance of apoptotic cells in vivo and in vitro: calreticulin and CDgl as a common collectin receptor complex显示文摘 | Vandivier RW Ogden CA Fadok VA | 2002 | J Immunol2002，169，7： | 1 |
| 20 | Remov efficiency and bind- ing mechanisms of copper and copper-EDTA complexes using po-lyethyleneimine显示文摘 | Maketon W Zenner C S Ogden K L | 2008 | Environmental Science and Technology2008，42，6： | 1 |
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