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| 1 | Mechanisms of regulation of PFKFB expression in pancreatic and gastric cancer cells显示文摘Enzymes 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 and-4(PFKFB-3 and PFKFB-4)play a significant role in the regulation of glycolysis in cancer cells as well as its proliferation and survival.The expression of these mRNAs is increased in malignant tumors and strongly induced in different cancer cell lines by hypoxia inducible factor(HIF)through active HIF binding sites in promoter region of PFKFB-4 and PFKFB-3 genes.Moreover,the expression and hypoxia responsibility of PFKFB-4 and PFKFB-3 was also shown for pancreatic(Panc1,PSN-1,and MIA PaCa-2)as well as gastric(MKN45 and NUGC3)cancer cells.At the same time,their basal expression level and hypoxia responsiveness vary in the different cells studied:the highest level of PFKFB-4 protein expression was found in NUGC3 gastric cancer cell line and lowest in Panc1 cells,with a stronger response to hypoxia in the pancreatic cancer cell line.Overexpression of different PFKFB in pancreatic and gastric cancer cells under hypoxic condition is correlated with enhanced expression of vascular endothelial growth factor(VEGF)and Glut1 mRNA as well as with increased level of HIF-1αprotein.Increased expression of different PFKFB genes was also demonstrated in gastric,lung,breast,and colon cancers as compared to corresponding nonmalignant tissue counterparts from the same patients,being more robust in the breast and lung tumors.Moreover,induction of PFKFB-4 mRNA expression in the breast and lung cancers is stronger than PFKFB-3mRNA.The levels of both PFKFB-4 and PFKFB-3 proteins in non-malignant gastric and colon tissues were more pronounced than in the non-malignant breast and lung tissues.It is interesting to note that Panc1and PSN-1 cells transfected with dominant/negative PFKFB-3(dnPFKFB-3)showed a lower level of endogenous PFKFB-3,PFKFB-4,and VEGF mRNA expressions as well as a decreased proliferation rate of these cells.Moreover,a similar effect had dnPFKFB-4.In conclusion,there is strong evidence that PFKFB-4 and PFKFB-3 isoenzymes are induced under hypoxia in pancreatic and other cancer cell lines,are overexpressed in gastric,colon,lung,and breast malignant tumors and undergo changes in their metabolism that contribute to the proliferation and survival of cancer cells.Thus,targeting these PFKFB may therefore present new therapeutic opportunities. | Oleksandr H Minchenko Katsuya Tsuchihara Dmytro O Minchenko Andreas Bikfalvi Hiroyasu Esumi | 2014 | World Journal of Gastroenterology2014，20，38： | 17 |
| 2 | Endothelin-1,endothelin receptors and ecNOS gene transcription in vital organs during traumatic shock in rats显示文摘 | Minchenko AG Armstead VE Opentanova IL | 1999 | Endothelium1999，6，： | 2 |
| 3 | Regulation of vascular endothelial growth factor(VEGF) gene transcription by estrogen receptors alpha and beta显示文摘 | MUELLER M D VIGNE J L MINCHENKO A | 2000 | Proc Natl Acad Sci USA2000，97，10： | 1 |
| 4 | Aldose reductase inhibitor fidarestat prevents retinal oxidative stress and vascular endothelial growth factor overexprcssion in streptozotocin diabetic rats显示文摘 | Minchenko AG Vasupuram R | 2003 | Diabetes2003，52，3： | 1 |
| 5 | P-selectin is up-regu-lated in vital organs during murine traumatic shock 显示文摘 | Armstead VE Minchenko AG Campbell B | 1997 | FASEB J1997，11，14： | 1 |
| 6 | Diabetes-induced overexpression of endothelin-1 and endothelin receptors in the rat renal cortex is mediated via poly(ADP-ribose) polymerase activation显示文摘 | Minchenko AG Stevens MJ White L | 2003 | FASEB J2003，17，： | 1 |
| 7 | Expression of phosphofibosyl pyrophosphate synthetase genes in U87 glioma cells with ERN1 knockdown:effect of hypoxia and endoplasmic reticulum stress 显示文摘 | MINCHENKO O H GARMASH I A KOVALEVSKA O V | 2014 | Ukr Biokhim Zh2014，86，6： | 1 |
| 8 | Antioxidants attenuate early up regulation of retinal vascular endothelial growth factor in streptozotocin-diabetic rats显示文摘 | Obrosova IG Minchenko AG Marinescu V Fathallah L Kennedy A Stockert CM | 2001 | Diabetologia2001，44，9： | 1 |
| 9 | Regulation of endothelin-1 gene expression in human microvascular endothelial cells by hypoxia and cobalt:role of hypoxia responsive element显示文摘 | Minchenko A Caro J | 2000 | Mol Cell Biochem2000，208，： | 1 |
| 10 | P-selectin is up-regulated in vital organs during murine traumatic shock显示文摘 | Minchenko AG | 1997 | FASEB J1997，11，14： | 1 |
| 11 | Aldose reductase inhibitor fidarestat prevents retinal oxidative stress and vascular endothelial growth factor overexpression in streptozotocin-diabetic rats显示文摘 | Obrosova IG Minchenko AG Vasupuram R White L Abatan OI Kumagai AK | 2003 | Diabetes2003，52，3： | 1 |
| 12 | Antioxidants attenuate early upregulation of retinal vascular endothelial growth factor in streptozotocin-diabetic rats显示文摘 | Obrosova IG Minchenko AG Marinescu V | 2001 | Diabetologia2001，44，9： | 1 |
| 13 | Regulation of vas-cular endothelial growth factor (VEGF) gene transcription by es-trogen receptors a and P显示文摘 | Mueller MD Vigne JL Minchenko A | 2000 | Proc Natl Acad Sci USA2000，97，10： | 1 |
| 14 | On relaxed constant rank regularity condition in mathematical programming 显示文摘 | Minchenko L Stakhovsji S | 2011 | Optimization2011，60，4： | 1 |
| 15 | Regulation of endothelin-1 gene expression in human microvascular cells by hypoxia and cobalt: role of hypoxia responsive element 显示文摘 | Minchenko A Caro J | 2000 | Mol-CellBiochem2000，208，12： | 1 |
| 16 | Effect of insulin on RNA and protein biosynthesis in liver mitochondria from normal and alloxan diabetic rats 显示文摘 | Germanyuk YL Minchenko AG | 1978 | Endocrinol Exp1978，12，4： | 1 |
| 17 | Regulation of endothelin-1 gene expression in human microvascular endothelial cells by hypoxia and cobalt:role of hypoxia responsive element显示文摘 | A Minchenko and J Caro | 2000 | Mol Cell Biochem2000，208，12： | 1 |
| 18 | Hy- poxic regulation of the 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase gene family ( PFKFB-17 4) expression in vivo显示文摘 | MINCHENKO O OPENTANOVA I CARO J | 2003 | FEBS Lett2003，554，3： | 1 |
| 19 | Diabetes-induced over-experession of endothelin-I and endothelin receptors in the rat renal cortex is mediated via poly(ADP-fibose) polymerase activation显示文摘 | Minchenko AG Stevens M J White L | 2003 | FASEB J2003，17，11： | 1 |
| 20 | P-selectin is up-regulated in vital organs during murine traumatic shock显示文摘 | Armstead VE Minchenko AG Campbell B | 1997 | FASEB J1997，11，14： | 1 |
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