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| 1 | Evaluation of clinical relevance of examining K-ras, p16 and p53 mutations along with allelic losses at 9p and 18q in EUS-guided fine needle aspiration samples of patients with chronic pancreatitis and pancreatic cancer显示文摘AIM: To establish an optimum combination of molecular markers resulting in best overall diagnostic sensitivity and specificity for evaluation of suspicious pancreatic mass. METHODS: Endoscopic ultrasound (EUS)-guided fine needle aspiration cytology (FNA) was performed on 101 consecutive patients (63 males, 38 females, 60 ± 12 years; 81 with subsequently diagnosed pancreatic cancer, 20 with chronic pancreatitis) with focal pancreatic mass. Samples were evaluated on-site by an experienced cytopathologist. DNA was extracted from Giemsa stained cells selected by laser microdissection and the presence of K-ras, p53 and p16 somatic mutations was tested by cycling-gradient capillary electrophoresis (CGCE) and single-strand conformation polymorphism (SSCP) techniques. In addition, allelic losses of tumor suppressor genes p16 (INK4, CDKN2A) and DPC4 (MADH4, SMAD4) were detected by monitoring the loss of heterozygosity (LOH) at 9p and 18q, respectively. RESULTS: Sensitivity and specificity of EUS-guided FNA were 75% and 85%, positive and negative predictivevalue reached 100%. The remaining 26% samples were assigned as inconclusive. Testing of molecular markers revealed sensitivity and specificity of 70% and 100% for K-ras mutations (P < 0.001), 24% and 90% for p53 mutations (NS), 13% and 100% for p16 mutations (NS), 85% and 64% for allelic losses at 9p (P < 0.001) and 78% and 57% for allelic losses at 18q (P < 0.05). When tests for different molecular markers were combined, the best results were obtained with K-ras + LOH at 9p (92% and 64%, P < 0.001), K-ras + LOH at 18q (92% and 57%, P < 0.001), and K-ras + LOH 9q + LOH 18q (96% and 43%, P < 0.001). When the molecular markers were used as complements to FNA cytology to evaluate inconclusive samples only, the overall sensitivity of cancer detection was 100% in all patients enrolled in the study. CONCLUSION: EUS-guided FNA cytology combined with screening of K-ras mutations and allelic losses of tumor suppressors p16 and DPC4 represents a very sensitive approach in screening for pancreatic malignancy. Molecular markers may find its use particularly in cases where FNA cytology has been inconclusive. | C Salek L Benesova M Zavoral V Nosek L Kasperova M Ryska R Strnad E Traboulsi M Minarik | 2007 | World Journal of Gastroenterology2007，13，27： | 18 |
| 2 | Colorectal cancer screening:20 years of development and recent progress显示文摘Colorectal cancer(CRC)is the second most common cancer in Europe and its incidence is steadily increasing.This trend could be reversed through timely secondary prevention(screening).In the last twenty years,CRC screening programs across Europe have experienced considerable improvements(fecal occult blood testing;transition from opportunistic to population based program settings).The Czech Republic is a typical example of a country with a long history of nationwide CRC screening programs in the face of very high CRC incidence and mortality rates.Each year,approximately 8000 people are diagnosed with CRC and some 4000 die from this malignancy.Twenty years ago,the first pilot studies on CRC screening led to the introduction of the opportunistic Czech National Colorectal Cancer Screening Program in 2000.Originally,this program was based on the guaiac fecal occult blood test(FOBT)offered by general practitioners,followed by colonoscopy in cases of FOBT positivity.The program has continuously evolved,namely with the implementation of immunochemical FOBTs and screening colonoscopy,as well as the involvement of gynecologists.Since the establishment of the Czech CRC Screening Registry in 2006,2405850 FOBTs have been performed and 104565 preventive colonoscopies recorded within the screening program.The overall program expanded to cover 25.0%of the target population by 2011.However,stagnation in the annual number of performed FOBTs lately has led to switching to the option of a population-based program with personal invitation,which is currently being prepared. | Miroslav Zavoral Stepan Suchanek Ondrej Majek Premysl Fric Petra Minarikova Marek Minarik Bohumil Seifert Ladislav Dusek | 2014 | World Journal of Gastroenterology2014，20，14： | 16 |
| 3 | Molecular biology of pancreatic cancer显示文摘In spite of continuous research efforts directed at early detection and treatment of pancreatic cancer, the outlook for patients affected by the disease remainsdismal. With most cases still being diagnosed at ad- vanced stages, no improvement in survival prognosis is achieved with current diagnostic imaging approaches. In the absence of a dominant precancerous condition, several risk factors have been identified including family history, chronic pancreatitis, smoking, diabetes mellitus, as well as certain genetic disorders such as hereditary pancreatitis, cystic fibrosis, familial atypical multiple mole melanoma, and Peutz-Jeghers and Lynch syn- dromes. Most pancreatic carcinomas, however, remain sporadic. Current progress in experimental molecular techniques has enabled detailed understanding of the molecular processes of pancreatic cancer development. According to the latest information, malignant pancre- atic transformation involves multiple oncogenes and tumor-suppressor genes that are involved in a variety of signaling pathways. The most characteristic aberrations (somatic point mutations and allelic losses) affect onco- genes and tumor-suppressor genes within RAS, AKT and Wnt signaling, and have a key role in transcription and proliferation, as well as systems that regulate the cell cycle (SMAD/DPC, CDKN2A/p16) and apoptosis (TP53). Understanding of the underlying molecular mechanisms should promote development of new methodology for early diagnosis and facilitate improvement in current ap- proaches for pancreatic cancer treatment. | Miroslav Zavoral Petra Minarikova Filip Zavada Cyril Salek Marek Minarik | 2011 | World Journal of Gastroenterology2011，17，24： | 6 |
| 4 | Longitudinal molecular characterization of endoscopic specimens from colorectal lesions显示文摘AIM: To compare molecular profiles of proximal colon, distal colon and rectum in large adenomas, early and late carcinomas. To assess feasibility of testing directed at molecular markers from this study in routine clinical practice. METHODS: A prospective 3-year study has resulted in the acquisition of samples from 159 large adenomas and 138 carcinomas along with associated clinical parameters including localization, grade and histological type for adenomas and localization and stage for carcinomas. A complex molecular phenotyping has been performed using multiplex ligation-dependent probe amplification technique for the evaluation of Cp G-islandmethylator phenotype(CIMP), PCR fragment analysis for detection of microsatellite instability and denaturing capillary electrophoresis for sensitive detection of somatic mutations in KRAS, BRAF, TP53 and APC genes.RESULTS: Molecular types according to previously introduced Jass classification have been evaluated for large adenomas and early and late carcinomas. An increase in CIMP+ type, eventually accompanied with KRAS mutations, was notable between large adenomas and early carcinomas. As expected, the longitudinal observations revealed a correlation of the CIMP+/BRAF + type with proximal location. CONCLUSION: Prospective molecular classification of tissue specimens is feasible in routine endoscopy practice. Increased frequency of some molecular types corresponds to the developmental stages of colorectal tumors. As expected, a clear distinction is notable for tumors located in proximal colon supposedly arising from the serrated(methylation) pathway. | Petra Minarikova Lucie Benesova Tereza Halkova Barbora Belsanova Stepan Suchanek Jiri Cyrany Inna Tuckova Jan Bures Miroslav Zavoral Marek Minarik | 2016 | World Journal of Gastroenterology2016，22，20： | 2 |
| 5 | Tribological Characterization of Commercial Pure Titanium Processed by Multi-Directional Forging显示文摘This work discusses tribological properties of commercial pure (CP) titanium processed by multi-directional forging (MDF) up to six passes at room temperature and 220 ℃. For this purpose, wear test was conducted by dry sliding pin-on-disk method on the initial and ultrafine grained samples using different stress magnitudes of 1, 1.5 and 2 MPa. The results showed that wear resistance of CP titanium increases after the first pass of MDF in comparison with the initial condition, irrespective of the applied normal stress. For example, the average wear rate of MDFed samples was decreased about 30% and 24%, after first pass at room temperature and 220 ℃, respectively. However, average wear rate of the samples processed by six MDF passes was reduced about 40% at lower normal loads;it was increased about 9% at higher ones as compared to the initial condition. It was also found that the dominated wear mechanisms were abrasive and delaminated at the lower stresses, while the delamination mechanism was intensified and a slight adhesion was observed during the higher applied normal loads. | I. Ansarian M. H. Shaeri M. Ebrahimi P. Minarik | 2019 | Acta Metallurgica Sinica(English Letters)2019，32，7： | 2 |
| 6 | Poststroke depression incidence and risk factors: an integrative literature review显示文摘 | Johnson JL Minarik PA Nystrom KV | 2006 | J Neurosci Nurs2006，38，4： | 1 |
| 7 | Poststroke depression incidence and risk factors:an integrative literature review显示文摘 | Johnson JL Minarik PA Nystrom KV | 2006 | J Neurosci Nuts2006，38，4： | 1 |
| 8 | Poststroke depression incidence and risk factors : an integrative literature review 显示文摘 | Johnson J J Minarik PA NYstrom KV | 2006 | J Neurosci Nurs2006，38，4： | 1 |
| 9 | Post stroke depression incidence and risk factors: An integrative literature review显示文摘 | Johnsoh JL Minarik PA Nystrom KV | 2006 | J Neurosei Nurse2006，38，4： | 1 |
| 10 | High-resolution melting analysis for genotyping of the myotonic dystrophy type 1 associated Alu insertion/deletion polymorphism显示文摘 | Jan Radvansky Peter Resko Milan Surovy Gabriel Minarik Andrej Ficek Ludevit Kadasi | 2009 | Analytical Biochemistry2009，，1： | 1 |
| 11 | Children With Respiratory Distress Treated With High-Flow Nasal Cannula显示文摘 | Spentzas T Minarik M Patters AB | 2009 | J Intensive Care Med2009，24，5： | 1 |
| 12 | Poststreke depression incidence and risk factors:an integrative literature re- view 显示文摘 | Johnson JL Minarik PA Nystrm KV | 2006 | J Neuresci Nurs2006，38，4： | 1 |
| 13 | Substantially Higher Corrosion Resistance in AE42 Magnesium Alloy through Corrosion Layer Stabilization by ECAP Treatment 显示文摘 | Minarik P Kral R Hadzima B | 2012 | Acta Physica Polonica2012，122，3： | 1 |
| 14 | Children with respiratory distress treated with high-flow nasal cannula显示文摘 | Spentas T Minarik M Patters AB | 2009 | J Intensive Care Med2009，24，5： | 1 |
| 15 | A very rare case of late diagnosis of cor triatriatum sinistrum显示文摘我们为动脉的高血压和永久 atrial 纤维性颤动象治疗一样为心失败与住院的历史介绍了一个 73 岁的病人并且被发现与二头齿在联合有英国管 triatriatum sinistrum 大动脉的阀门。耐心的拒绝的外科的修正,而是他的条件为心失败和 atrial 纤维性颤动在保守治疗上改善了。 | lngrid Schusterova Martin Czemy Alexander Jurko Jr Milan Minarik | 2015 | Journal of Geriatric Cardiology2015，12，2： | 1 |
| 16 | Statutory and regulatory issues for clinical nurse specialist (CNS) practice : ensuring the public's access to CNS services 显示文摘 | Lyon BL Minarik PA | 2001 | Clinical Nurse Specialist2001，15，3： | 1 |
| 17 | Poststroke depression incidence and risk factors:an integrative fiterature review显示文摘 | Minarik PA Nystrom KV | 2006 | Neurosic Nurs2006，38，4： | 1 |
| 18 | Poststroke de pression incidence and risk factors: an integrative literature re view显示文摘 | Johnson JL Minarik PA Nystrm KV | 2006 | J NeurosciNurs2006，38，4： | 1 |
| 19 | 10years of experience with thalidomide in multiple myeloma patients:report of the CzechMyeloma Group显示文摘 | Minarik J Sandecka V Maisnar V | 2013 | Leuk Res2013，37，9： | 1 |
| 20 | Post-stroke Depression Incidence and Risk Factors:An Integrative Literature Review显示文摘 | Johnson JI Minarik PA Nystrm KV eta1 | 2006 | JNeumsci Nuts2006，38，： | 1 |
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