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| 1 | Examining relationships between particle counts and giardia, cryptosporidium,and turbidity显示文摘 | Mar W LeChevallier William D Norton | 1992 | J AWWA1992，84，12： | 2 |
| 2 | Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells显示文摘BACKGROUND Human adipose-derived stromal/stem cells(hASCs)are one of the most useful types of mesenchymal stromal/stem cells,which are adult multipotent cells with great therapeutic potential for the treatment of several diseases.However,for successful clinical application,it is critical that high-quality cells can be obtained.Diverse factors seem to be able to influence cell quality and performance,especially factors related to donors’intrinsic characteristics,such as age.Nevertheless,there is no consensus regarding this characteristic,and there is conflicting information in the literature.AIM To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young donors.METHODS hASCs were harvested from liposuctioned adipose tissue obtained from female donors(aged 20-70 years).Cells were distributed into two groups according to age range:old hASCs(oASCs,≥55 years,n=9)and young hASCs(yASCs,≤35 years,n=9).For each group,immunophenotypic characterization was performed by flow cytometry.Population doubling time was assessed over seven days.For adipogenic potential evaluation,lipid deposits were assessed after 7 d,14 d and 21 d of adipogenic induction.Osteogenic potential was verified by analyzing cell mineralization after 14 d,21 d and 28 d of osteogenic induction.mRNA expression of PPARγ2,CEBPA and Runx2 were detected by quantitative reverse transcription polymerase chain reaction.RESULTS hASCs were successfully obtained,cultured,and grouped according to their age:yASCs(26.33±4.66 years old)and oASCs(64.78±4.58 years old).After maintenance of the cells in culture,there were no differences in morphology between cells from the young and old donors.Additionally,both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal cells.The average doubling time indicated that yASCs(4.09±0.94 d)did not significantly differ from oASCs(4.19±1.29 d).Concerning differentiation potential,after adipogenic and osteogenic induction,yASCs and oASCs were able to differentiate to greater levels than the noninduced control cells.However,no differences were found in the differentiation efficiency of yASCs and oASCs in adipogenesis or osteogenesis.Additionally,the mRNA expression of PPARγ2,CEBPA and Runx2 were similar in yASCs and oASCs.CONCLUSION Our findings suggest that age does not seem to significantly affect the cell division or adipogenic or osteogenic differentiation ability of adipose-derived stem cells isolated from lipoaspirates. | Cintia DS Horinouchi María Julia Barisón Anny W Robert Crisciele Kuligovski Alessandra M Aguiar BrunoDallagiovanna | 2020 | World Journal of Stem Cells2020，12，12： | 2 |
| 3 | A Literature Survey on Fatigue Analy- sis Approaches for Rubber显示文摘 | Mars W V Fatemi A | 2002 | International Journal of Fa- tigue2002，24，4： | 1 |
| 4 | A Literature Survey on Fatigue Analy- sis Approaches for Rubber显示文摘 | Mars W V Fatemi A | 2002 | International Journal of Fa- tigue2002，24，9： | 1 |
| 5 | A phenomenological model for the effect of R ratio on fatigue of strain crystallizing rubbers显示文摘 | Mars W V Fatemi A | 2003 | Rubber Chemistry and Technology2003，76，5： | 1 |
| 6 | Calculation of the roal of coal constituent elements on the enhanced vaporization of silica显示文摘 | NAGELBERG A S MAR R W CARLING R W | 1985 | High Temperature Science1985，19，1： | 1 |
| 7 | Cracking energy density as a predictor of fatigue lifeunder multiaxial conditions 显示文摘 | Mars W V | 2002 | Rubber Chemistry and Tech- nology2002，75，1： | 1 |
| 8 | Tin-transition metal-carbon systems for lithium-ion battery negative electrodes显示文摘 | Todd A D W Mar R E Dahn J R | 2007 | Journal of the Electrochemical Society2007，154，6： | 1 |
| 9 | A literature survey on fatigue analysis approaches for rubber 显示文摘 | Mars W V Fatemi A | 2002 | International Journal of Fatigue2002，24，9： | 1 |
| 10 | Evidence for differential cortical input to direct pathway versus indirect pathway striatal projection neurons in rats 显示文摘 | Lei W L Jiao Y Del Mar N | 2004 | J Neurosci2004，24，38： | 1 |
| 11 | Telomere shortening and apoptosis in telomerase-inhibited human tumor cells显示文摘 | Zhang X Mar V Zhou W | 1999 | Genes Dev1999，13，18： | 1 |
| 12 | Pollutant loading model fo- rhighway runoff 显示文摘 | Chui T W Mar B W Homer R R | 1982 | Journal of Environmental Engineering1982，108，6： | 1 |
| 13 | A sensitive new substrate for chymotrypsin 显示文摘 | Del Mar E G Largman C Brodrick J W | 1979 | Analytical Biochemistry1979，99，2： | 1 |
| 14 | Factors that Affect the Fatigue Life of Rubber:A Literature Survey显示文摘 | Mars W V | 2004 | Rubber Chemistry and Technology2004，77，3： | 1 |
| 15 | Fatigue Crack Nucleation and Growth in Filled Natural Rubber显示文摘 | Mars W V Fatemi A | 2003 | Fatigue & Fracture of Engineer- ing Materials & Structures2003，26，9： | 1 |
| 16 | Combinatorial study of tin-transition metal alloys as negative electrodes for lithiumion batteries显示文摘 | Todd A D W Mar R E Dahn J R | 2006 | J Electrochem Soc2006，153，10： | 1 |
| 17 | Multiaxial Fatigue Crack Initiation in Rubber显示文摘 | Mars W V | 2001 | Tire Science and Technology2001，29，3： | 1 |
| 18 | A Phenomenological Model for the Effect of R Ratio on Fatigue of Strain Crystallizing Rubbers 显示文摘 | Mars W V Fatemi A | 2003 | Rubber Chemistry and Technology2003，76，5： | 1 |
| 19 | Cracking Energy Density as a Predictor of Fatigue Life under Multiaxial Conditions显示文摘 | Mars W V | 2002 | Rubber Chemistry and Technology2002，75，1： | 1 |
| 20 | Factors that Affect the Fatigue Life of Rubber: A Literature Survey显示文摘 | Mars W V | 2004 | Rubber Chemistry and Technolo- gy2004，77，3： | 1 |
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