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46篇 您的检索式:作者名="Manshardt"
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1Tissue differential expression of lycopene β-cyclase gene in papaya显示文摘Carotene pigments in flowers and fruits are distinct features related to fitness advantages such as attracting insects forpollination and birds for seed dispersal.In papaya,the flesh color of the fruit is considered a quality trait that correlateswith nutritional value and is linked to shelf-life of the fruit.To elucidate the carotenoid biosynthesis pathway in papaya,we took a candidate gene approach to clone the lycopene β-cyclase gene,LCY-B.A papaya LCY-B ortholog,cpLCY-B,was successfully identified from both cDNA and bacterial artificial chromosome(BAC)libraries and complete genomicsequence was obtained from the positive BAC including the promoter region.This cpLCY-B shared 80% amino acididentity with citrus LCY-B.However,full genomic sequences from both yellow- and red-fleshed papaya were identical.Quantitative real-time PCR(qPCR)revealed similar levels of expression at six different maturing stages of fruits forboth yellow-and red-fleshed genotypes.Further expression analyses of cpLCY-B showed that its expression levels wereseven- and three-fold higher in leaves and,respectively,flowers than in fruits,suggesting that cpLCY-B is down-regulatedduring the fruit ripening process.Rachel L Skelton Qingyi Yu Rajeswari Srinivasan Richard Manshardt Paul H Moore Ray Ming 2006Cell Research2006,16,8:8
2Stable transformation of Papaya via microprojectile bombardment 显示文摘FITCH M M M MANSHARDT R M GONSALVES D 1990Plant Cell Report1990,9,:1
3Pathogen-derived resistance provides papaya with effective protection against papaya ringspot virus显示文摘Lius S Manshardt R M Fitch M M 1997Molecular Breeding1997,3,:1
4Virus resistant papaya plants derived from tissues bombarded with the coat protein gene of papaya ringspot rirus显示文摘Fitch M M Manshardt R M Gousalves D 1992Bio/Technology1992,10,:1
5Virus resistant papaya plants derived from tissues bombarded with the coat protein gene of papaya ringspot virus显示文摘Fitch M M M Manshardt R M Gonsalves D 1992Bio Technology1992,10,:1
6Stable transformation of papaya via microprojectile bombardment显示文摘Fitch MMM Manshardt RM Gonsalves D 1990Plant Cell Rep1990,9,:1
7Differential protection against papaya ringspot virus isolates in coat protein gene transgenic papaya and classically cross-protected papaya显示文摘 Gonsalves C Ling K-S Fitch M Manshardt R Siightom JL Gonsalves D 1994Phytopa thology1994,64,:1
8Virus resistant papaya plants derived from tissues bombarded with the coat protein gene of papaya ringspot virus显示文摘Fitch MMM Manshardt RM Gonsalves D 1992Bio/Technol1992,10,:1
9A genetic linkage map of papaya based on randomly amplified polymorphic DNA markers显示文摘Sondur S N Manshardt R M Stiles J I 1996Theoretical and Applied Genetics1996,93,4:1
10Virus coat protein transgenic Papaya provides practical control of Papaya ringspot virus in Hawaii 显示文摘Ferreira S A Pitz K Y Manshardt R 2002Plant Disease2002,86,2:1
11Virus coat protein transgenic papaya provides practical control of papaya ringspot virus in Hawaii显示文摘Ferreira S A Pitz K Y Manshardt R etal 2002Plant Dis2002,86,2:1
12Biotechnology of papaya显示文摘Manshardt R M Drew R A 1998Acta Horticulturae1998,461,:1
13Stable transformation of papaya via microprojeetile bombardmen显示文摘FITCH M M M MANSHARDT R M GONSALVES D eta 1990Plant Cell Reports1990,9,4:1
14Virus resistant papaya plants derived from tissues bombarded with the coat protein gene of papaya ringspot virus显示文摘FITCH M M M MANSHARDT R M GONSALVES D 1992Bio Technology1992,10,:1
15Biotechnology of papaya显示文摘Manshardt R M and Drew R A 1998Acta Horticulturae1998,461,:1
16Virus resistant papaya plants derives from tissues bombarded with the coat protein gene of papaya a ringspot virus显示文摘Fitch M M Manshardt R V Gonsalves D el al 1992Bio/Technol1992,10,:1
17A genetic linkage map of papaya based on randomly amplified polymorphic DNA markers 显示文摘Sondur S N Manshardt R M Stiles J I 1996Theor Appl Genet1996,93,:1
18Stable Wansformation of papaya via microp mjectile bombardment 显示文摘Fitch MMM Manshardt RM Gonsalves D 1990Plant Cell Reports1990,,9:1
19Virus resistant papaya plants derives from tissues bombarded with the coat protein gene of papaya ringspot virus显示文摘Fitch M M Manshardt R M Gonsalves D 1992Nature Biotectmology1992,10,:1
20Virus resistant papaya plants derived from tissoes bombarded with the CP gene of PRV显示文摘Fitch MMM Manshardt RM Gonsalves D 1992Bin/Technology1992,10,:1
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