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| 1 | Analysis of DNA methylation in different maize tissues显示文摘DNA methylation plays an important role in gene expression regulation during biological development and tissue differentiation in plants. This study adopted methylation-sensitive Amplified fragment length polymorphism (AFLP) to compare the levels of DNA cyto- sine methylation at CCGG sites in tassel, bracteal leaf, and ear leaf from maize inbred lines, 18 White and 18 Red, respectively, and also examined specific methylation patterns of the three tissues. Significant differences in cytosine methylation level among the three tissues and the same changing tendency in two inbred lines were detected. Both MSAP (methylation sensitive amplification polymorphism) ratio and full methylation level were the highest in bracteal leaf, and the lowest in tassel. Meanwhile, different methylation levels were ob- served in the same tissue from the inbred lines, 18 White and 18 Red. Full methylation of internal cytosine was the dominant type in the maize genome. The differential methylation patterns in the three tissues were observed. In addition, sequencing of nine differentially me- thylated fragments and the subsequent blast search revealed that the cytosine methylated 5′-CCGG-3′sequences were distributed in re- peating sequences, in the coding and noncoding regions. Southern hybridization was used to verify the methylation polymorphism. These results clearly demonstrated the power of the MSAP technique for large-scale DNA methylation detection in the maize genome, and the complexity of DNA methylation change during plant growth and development. The different methylation levels may be related to specific gene expression in various tissues. | Yanli Lu, Tingzhao Rong, Moju Cao Maize Research Institute of Sichuan Agriculture University/Key Laboratory of Crop Genetic Resource and Improvement, Ministry of Education, Ya’an, 625014, China | 2008 | Journal of Genetics and Genomics2008，35，1： | 27 |
| 2 | Relationship between differ- ential gene expression pat- terns in functional leaves of maize (Zea mays L.) at milk filling stage and heterosis using cDNA-AFLP显示文摘To understand the molecular mechanism of maize heterosis, differential gene expression patterns in the functional leaves of 35 maize hybrids relative to their parents involving 10 elite inbreds at milk filling stage were analyzed by using cDNA-AFLP. The correlation analyses of various differential expression patterns with the performance and heterosis of main maize agronomic traits were evaluated. The main results were as follows: For uniparental specific expression, significant positive correlations were detected with the performance of seed weight per ear and 100-seed weight at 0.01 and 0.05 probability levels respectively. For biparental specific expression, significant negative correla-tions were detected with the performance of ear diameter and seed weight per ear at 0.01 probability level. For uni-parental specific expression, significant positive correlations were detected with the heterosis of ear diameter and seed weight per ear at 0.01 and 0.05 probability levels respectively. For biparental specific expression, significant negative cor-relation was detected with the heterosis of ear diameter at 0.05 probability level. However, for F1-specific expression, for fragments detected only in one parent and F1, and for fragments detected only in two parents or only in F1, no sig-nificant correlation was detected with the performance or heterosis of all agronomic traits surveyed. | TIAN Zengyuan & DAI Jingrui National Maize Improvement Center of China, China Agricultural Uni-versity, Beijing 100094, China Correspondence should be addressed to Tian Zengyuan (e-mail: tian-zengyuan@yahoo.com.cn) | 2003 | Chinese Science Bulletin2003，48，1： | 10 |
| 3 | Cloning and characterization of a novel R1-MYB transcription factor in maize显示文摘R1-MYB transcription regulatory factors play vital roles in transcriptional modification during higher plant metabolism and development. In the present study, an R1-MYB gene was isolated from the maize (Zea mays L.) inbred line C8605-2 based on expressed sequence tags (ESTs), which expressed differentially between the hybrid C8605-2 × W1445 and its two parents in an oligonucleotide microarray. The full-length cDNA, designated as ZmMYBE1 (GeneBank Accession No. FJ024049), consists of 1992 nucleotides and contains a 1296-bp open reading frame. One conserved MYB domain was detected near the N-terminus of the deduced amino acid sequence, where an acidic Ser/Thr-rich area was also observed in the downstream region. The sequence of ZmMYBE1, mapped to chromosome 5 (bin 5.03), was revealed belonging to a multi-gene family in the maize genome. Subcellular localization analysis determined that ZmMYBE1 was located in the nucleus. Expression analysis showed that ZmMYBE1 transcripts accumulated in various tissues examined, with high expression levels in the immature ear and low levels in the tassel, root, and stem. The expression analysis for ZmMYBE1 in immature ears showed the highest expression level at the earliest vegetative developmental stage of the plant. Expression of ZmMYBE1 higher than that of the parents was observed in the hybrid C8605-2 × W1445. And also the expression level similar to the lowest of the parents in the hybrid C8605-2 × W245 was observed, revealing different expression patterns for the same gene in various hybrids combined from diversified inbred lines. Transgenic Arabidopsis overexpressing ZmMYBE1 implies that ZmMYBE1 has an important role in developmental regulation in maize. | Guanqing Jia, Bo Li, Dengfeng Zhang, Tifu Zhang, Zhiyong Li, Jingrui Dai, Shoucai Wang* National Maize Improvement Center of China, Key Laboratory of Crop Genomics and Genetic Improvement of Agriculture Ministry, Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China | 2009 | Progress in Natural Science:Materials International2009，19，9： | 6 |
| 4 | The evolution of integrative medical education: the influence of the University of Arizona Center for Integrative Medicine显示文摘The University of Arizona Center for Integrative Medicine(Az CIM) was founded in 1994 with a primary focus of educating physicians in integrative medicine(IM). Twenty years later, IM has become an internationally recognized movement in medicine. With 40% of United States' medical schools having membership in the Academic Consortium for Integrative Medicine and Health it is foreseeable that all medical students and residents will soon receive training in the principles and practices of IM. The Az CIM has the broadest range and depth of IM educational programs and has had a major infl uence on integrative medical education in the United States. This review describes the fellowship, residency and medical student programs at Az CIM as well as other signifi cant national drivers of IM education; it also points out the challenges faced in developing IM initiatives. The fi eld of IM has matured with new national board certifi cation in IM requiring fellowship training. Allied health professional IM educational courses, as well as integrative health coaching, assure that all members of the health care team can receive training. This review describes the evolution of IM education and will be helpful to academic centers, health care institutions, and countries seeking to introduce IM initiatives. | Victoria Maizes Randy Horwitz Patricia Lebensohn Hilary McClafferty James Dalen Andrew Weil | 2015 | Journal of Integrative Medicine2015，13，6： | 5 |
| 5 | A Classification of 45 Maize Inbred Lines Used in China with RFLP Markers显示文摘The classification of heterotic groups is essential to maize breeding because knowledge of heterotic groups could be interest to both the combination of outstanding hybrids and the improvement of elite inbred lines. RFLP has provided a powerful tool to assign maize inbred lines into heterotic groups. In this investigation, 45 inbred lines, used widely in south and southwest China, were chosen for RFLP analysis,among which 4 lines came from American, representing different heterotic groups in U.S. corn belt. 54 RFLP core markers covering 10 chromosomes of maize were used. A total DNA of each sample was digested with EcoR I, BamH Ⅰ and Hind Ⅲ . The procedure of RFLP was employed as described by a manual from maize RFLP lab at University of Missouri, Columbia. A total of 860 bands were detected among 45 inbred lines based on RFLP analysis, which were involved in 212 loci. Alleles at each locus ranged from 2 to 9 with an average of 4.06. In total, The 45 inbred lines were classified into 6 heterotic groups according to RFLP data with Ward's method. 3 heterotic groups, including Mo17, B73 and Oh43 respectively, seemed to be the same to U. S. heterotic groups. 21 inbred lines, most of which derived from Chinese local germplasm, were classified together into two heterotic groups, indicating domistic germplasm was different from U. S.germplasm at the molecular level and played an important role in maize hybrid production in China. Two inbred lines from tropic germplasm were assigned in the same group. These results provided useful information for our understanding maize heterotic groups and heterotic patterns in China. | HUANG Yi-qin and LI Jian-sheng( National Key Lab of Crop Genetic Improvement, Huazhong Agricultural University , Wuhan 430070 National Maize Improvement Center of China , China Agricultural University, Beijing 100094) | 2002 | Agricultural Sciences in China2002，1，2： | 4 |
| 6 | A genome-wide survey of maize lipid-related genes: candidate genes mining,digital gene expression profiling and colocation with QTL for maize kernel oil显示文摘Lipids play an important role in plants due to their abundance and their extensive participation in many metabolic processes.Genes involved in lipid metabolism have been extensively studied in Arabidopsis and other plant species.In this study,a total of 1003 maize lipid-related genes were cloned and annotated,including 42 genes with experimental validation,732 genes with full-length cDNA and protein sequences in public databases and 229 newly cloned genes.Ninety-seven maize lipid-related genes with tissue-preferential expression were discovered by in silico gene expression profiling based on 1984483 maize Expressed Sequence Tags collected from 182 cDNA libraries.Meanwhile,70 QTL clusters for maize kernel oil were identified,covering 34.5% of the maize genome.Fifty-nine (84%) QTL clusters co-located with at least one lipid-related gene,and the total number of these genes amounted to 147.Interestingly,thirteen genes with kernel-preferential expression profiles fell within QTL clusters for maize kernel oil content.All the maize lipid-related genes identified here may provide good targets for maize kernel oil QTL cloning and thus help us to better understand the molecular mechanism of maize kernel oil accumulation. | LI Lin1,LI Hui1,LI JiYing1,XU ShuTu1,YANG XiaoHong1,LI JianSheng1 & YAN JianBing1,2 1National Maize Improvement Centre of China,China Agricultural University,Beijing 100193,China 2International Maize and Wheat Improvement Centre (CIMMYT),Apdo.Postal 6-641,06600 Mexico,D.F.,Mexico | 2010 | Science China(Life Sciences)2010，53，6： | 2 |
| 7 | Evaluation of heavy metal availability in polluted soils by two sequential extraction procedures using factor analysis显示文摘 | I Maiz I Arambarri R Garcia E Millán | 2000 | Environmental Pollution2000，，1： | 2 |
| 8 | Ductus venosus Doppler at 11 to 13 weeks of gestation in the prediction of outcome in twin pregnancies显示文摘 | Maiz N Staboulidou I Leal AM | | 0，，04： | 1 |
| 9 | Uterine artery Doppler at 11 +0 to 13 +6 weeks and 21 +0 to 24 +6 weeks in the prediction of preeclampsia显示文摘 | Plasencia W Maiz N Poon L | | 0，，02： | 1 |
| 10 | Evaluation of heavy metal availability in polluted soils by two sequential extraction procedures using factor analysis显示文摘 | Maiz I Arambarri I Garcia R | 2000 | Environmental Pollution2000，110，1： | 1 |
| 11 | Choroid plexus cyst,intracardiac echogenic focus, hyperechogeuic bowel and hydronephrosis in screening for trisomy 21 at 11^+0 to 13^+6 weeks 显示文摘 | Dagklis T Plasencia W Maiz N | 2008 | Ultrasound Obstet Gynecol2008，31，2： | 1 |
| 12 | Morsicatio buecarum et labiorum (excessive cheek and lip biting) 显示文摘 | Glass LF Maize JC | 1991 | Am J Dermatopathol1991，13，3： | 1 |
| 13 | Incidental epidermolytic hyperkeratosis and focal acantholytic dyskeratosis in common acquired melanocytic nevi and atypical melanocytic lesions显示文摘 | Hutcheson AC Nietert PJ Maize JC | 2004 | J Am Acad Dermatol2004，50，3： | 1 |
| 14 | Evaluation of heavy metal availability in polluted soils by two sequential extraction procedures using factor analysis显示文摘 | Maiz I Ar ambar ri I Garcia R | 2000 | Environmental Pollution2000，110，1： | 1 |
| 15 | Evaluation of heavy metal availability in polluted soils by two sequential extraction procedures using factor analysis显示文摘 | Maiz I Arambarri I Garcia R Millán E | 2000 | Environmental Pollution2000，110，： | 1 |
| 16 | First-trimesterprediction of hypertensive disorders in pregnancy 显示文摘 | Poon L C Kametas N A Maiz N | 2009 | Hypertension2009，53，5： | 1 |
| 17 | Duetus venosus Doppler in fetu- ses with cardiac defects and increased nuchal translucency thickness 显示文摘 | Maiz N Plasencia W Dagklis T | 2008 | Ultrasound Obstet Gyneeol2008，31，2： | 1 |
| 18 | Heavy metal contamination analysis of road soils and grasses from Gipuzkoa(Spain)显示文摘 | Garcia R Maiz I Millan E | 1996 | Environmental Technology1996，17，7： | 1 |
| 19 | Ductus venosus Doppler in screening for trisomies 21,18 and 13 and Turner syndrome at 11 - 13 weeks of gestational 显示文摘 | Maiz N Valencia C Kagan KO | 2009 | Ultrasound Obstet Gynecol2009，33，： | 1 |
| 20 | Evaluation of heavymetal availability in polluted soils by two sequential extraction procedures using factor analysis显示文摘 | Maiz I Ar ambarri I Garcia R | 2000 | Environmental Pollution2000，110，1： | 1 |
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