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| 1 | Impaired norepinephrine regulation of monocyte inflammatory cytokine balance in heart failure显示文摘AIM To evaluate the effect of norepinephrine on inflammatory cytokine expression in ex vivo human monocytes and monocytic THP-1 cells. METHODS For human monocyte studies, cells were isolated from 12 chronic heart failure(HF)(66 ± 12 years, New York Heart Association functional class Ⅲ-Ⅳ, left ventricular ejection fraction 22% ± 9%) and 14 healthy subjects(66 ± 12 years). Monocytes(1 × 106/mL) were incubated with lipopolysaccharide(LPS) 100 ng/m L, LPS + norepinephrine(NE) 10-6 mol/L or neither(control) for 4 h. Tumor necrosis factor-alpha(TNFα) and interleukin-10(IL-10) production were determined by ELISA. Relative contribution of α- and β-adrenergic receptor subtypes on immunomodulatory activity of NE was assessed in LPSstimulated THP-1 cells incubated with NE, the α-selective agonist phenylephrine(PE), and the β-selective agonist isoproterenol(IPN). NE-pretreated THP-1 cells were also co-incubated with the β-selective antagonist propranolol(PROP), α2-selective antagonist yohimbine(YOH) or the α1-selective antagonist prazosin(PRAZ). RESULTS Basal TNFα concentrations were higher in HF vs healthy subjects(6.3 ± 3.3 pg/mL vs 2.5 ± 2.6 pg/mL, P = 0.004). Norepinephrine's effect on TNFα production was reduced in HF(-41% ± 17% HF vs -57% ± 9% healthy, P = 0.01), and proportionately with NYHA FC. Increases in IL-10 production by NE was also attenuated in HF(16% ± 18% HF vs 38% ± 23% healthy, P = 0.012). In THP-1 cells, NE and IPN, but not PE, induced a dosedependent suppression of TNFα. Co-incubation with NE and antagonists revealed a dose-dependent inhibition of the NE suppression of TNFα by PROP, but not by YOH or PRAZ. Dose-dependent increases in IL-10 production were seen with NE and IPN, but not with PE. This effect was also antagonized by PROP but not by YOH or PRAZ. Pretreatment of cells with IPN attenuated the effects of NE and IPN, but did not induce a response to PE.CONCLUSION NE regulation of monocyte inflammatory cytokine production may be reduced in moderate-severe HF, and may be mediated through β-adrenergic receptors. | Tien MH Ng Myron L Toews | 2016 | World Journal of Cardiology2016，8，10： | 4 |
| 2 | Prokaryotical expression of structural and non-structural proteins of hepatitis G virus显示文摘AIM To study the epitope distribution of hepatitis G virus (HGV) and to seek for the potential recombinant antigens for the development of HGV diagnositic reagents.METHODS Fourteen clones encompassing HGV gene fragments from core to NS3 and NS5 were constructed using prokaryotic expression vector pRSET and (or)pGEX. and expressed in E. coli. Western blotting and ELISA were used to detect the immunoreactivity of these recombinant proteins.``RESULTS One clone with HGV fragment from core to El(Gl). one from E2 (G31), three from NS3 (G6, G61, G7),one from NS5B (G821) and one chimeric fragment from NS3and NS5B (G61 821) could be expressed well and showed obvious immunoreactivity by Western blotting.One clone with I-KGV framment from NS5B (G82) was also well expressed, but could not show immunoreactivity by Western blotting. No obvious expression was found in the other six clones. All the expressed recombinant proteins were in inclusion body form, except the protein G61 which could be expressed in soluble form. Further purified recombinant proteins Gl, G,31, G61, G821 and G61 821were detected in indirected ELISA as coating antigen respectively. Only recombinant Gl could still show immunoreactivity, and the other four recombinant proteins failed to react to the HGV antibody positive sera.Western blotting results indicated that the immunoactivity of these four recombinant proteins were lost during purification.``CONCLUSION Core to El, E2. NS3 and NS5 fragment of HGV contain antigenic epitopes, which could be produced in prokaryotically expressed recombinant proteins. A high. yield recombinant protein (Gl) located in HGV core to E1 could remain its epitope after purification, which showed the potential that G1 could be used as a coating antigen to develop an ELISA kit for HGV specific antibody diagnosis. | Ning-Shao Xia~1 Hai-Jie Yang~1 Jun Zhang~1 Chang-Qing Lin~1 Ying-Bin Wang~1 Juan Wang~1 Mei-Yun Zhan~2 MH Ng~3 1 Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering,Xiamen University,Xiamen 361005,Fujian Province,China2 Institute of Virology,Chinese Academy of Preventive Medicine Beijing 100052,China3 Department of Microbiology,Hoog Kong University,Hongkong,China | 2001 | World Journal of Gastroenterology2001，7，5： | 4 |
| 3 | Association of polymorphism of human leukocyte antigen aleles with development of hepatocellular carcinoma in Hong Kong Chinese显示文摘 | Ng MH Cheng SH Lai PB | 2012 | Hong Kong Med J2012，18，6： | 1 |
| 4 | Age and gender effect on the growth of bone marrow stromal cells in vitro显示文摘 | Shamsul BS Aminuddin BS Ng MH | 2004 | Med J Malaysia2004，59，： | 1 |
| 5 | Immunoglobulin A against viral capsid antigen of Epstein-Barr virus and indirect mirror examination of the nasopharynx in the detection of asymptomatic nasopharyngeal carcinoma显示文摘 | ZONG YS SHAM JS NG MH | 1992 | Cancer1992，69，： | 1 |
| 6 | Frequent death-associated protein kinase promoter hypermethylation in multiple myeloma显示文摘 | Ng MH To KW Lo KW | 2001 | Clin Cancer Res2001，7，6： | 1 |
| 7 | Frequent death-associated protein kinase promoter hypermethylation in multiple myeloma显示文摘 | Ng MH To KW Lo KW Chan S Tsang KS Cheng SH | 2001 | Clin Cancer Res2001，7，： | 1 |
| 8 | Side population in human lung cancer cell lines and tumors is enriched with stem - like cancer cells显示文摘 | Ho MH Ng AV Lam S | 2007 | Cancer Res2007，67，： | 1 |
| 9 | A single dose of oral DNA im- munization delivered by attenuated Salmonella typhimurium dows- regulated transgene expression in HBsAg transgenie miee 显示文摘 | Zheng BJ Ng MH ChanKW | 2002 | Eur J Immunol2002，32，11： | 1 |
| 10 | Expanding the clinical,radiological and neuropathological phenotype of chronic lymphocytic inflammation with pontine perivascular en- hancement responsive to steroids (CLIPPERS)显示文摘 | SIMON NG PARRATT JD BARNETT MH | 2012 | J Neurol Neuro- surg Psychiatry2012，83，： | 1 |
| 11 | Doxycycline inhibits matrix metalloproteinase-2 secretion from TSC2-null mouse embryonic fibroblasts and lymphangioleiomyoma- tosis cells显示文摘 | Moir LM Ng HY Poniris MH | 2011 | Br J Pharmacol2011，164，1： | 1 |
| 12 | A comparison of immunological methods for the detection of Trichinella spiralis antigen显示文摘 | Choy WF Lim PL Ng MH | 1988 | J Immunol Methods1988，113，1： | 1 |
| 13 | Establishment and characterization of a cytogenetically complex Chinese multiple myeloma-derived cell line with homozygous p53 deletion and cyclin E overexpression显示文摘 | Cheng SH Ng MH Tsang KS | 2004 | Int J Oncol2004，24，5： | 1 |
| 14 | Establishment and characterization of a cytogenetically complex Chinese multiple myeloma-derived cell line with homozygous p53 deletion and cyclin E overexpression显示文摘 | Cheng SH Ng MH Tsang KS | 2004 | Int J Oncol2004，24，5： | 1 |
| 15 | Potentiation of mitogenic response by extract of the swiftlet's (Collocalia) nest显示文摘 | Ng MH Chan KH Kong YC | 1986 | Bl''ochem 7nt1986，13，： | 1 |
| 16 | Immunosensor for the differentiation and detection of Salmonella species based on a Quartz Crystal Microbalance显示文摘 | Wong YY Ng SP Ng MH | 2002 | Biosens Bioelectron2002，17，： | 1 |
| 17 | Frequent hypermethylation of p16 and p15 genes in multiple myeloma显示文摘 | Ng MH Chung YF Lo KW | 1997 | Blood1997，89，7： | 1 |
| 18 | Thrombopoietin levels increased in patients with severe acute respiratory syndrome显示文摘 | Yang M Ng MH Li CK | 2008 | Thromb Res2008，122，： | 1 |
| 19 | Association between HLA-B*58:01 allele and severe cutaneous adverse reactions with allopurinol in Han Chinese in Hong Kong显示文摘 | Chiu ML Hu M Ng MH | 2012 | Br J Dermatol2012，167，1： | 1 |
| 20 | Immunoglobulin A against Viral Capsid antigen of Epstein' Barr Virus and indirect mirror examina- tion of the nasopharynx in the detection of asymptomatic nasopha- ryngeal carcinoma 显示文摘 | Zong YS Sham JS Ng MH ct al | 1992 | Cancer1992，69，： | 1 |
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