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| 1 | Aggressive behaviour of solid-pseudopapillary tumor of the pancreas in adults:A case report and review of the literature显示文摘Solid-pseudopapillary tumor (SPT) is a rare neoplasm of the pancreas that usually occurs in young females. It is generally considered a low-grade malignant tumor that can remain asymptomatic for several years. The occurrence of infiltrating varieties of SPT is around 10%-15%. Between 1986 and 2006, 282 cystic tumors of the pancreas were observed. Among them a SPT was diagnosed in 8 patients (2.8%) with only one infiltrating variety. This was diagnosed in a 49-year-old female 13 years after the sonographic evidence of a small pancreatic cystic lesion interpreted as a pseudocyst. The tumor invaded a long segment of the portal- mesenteric vein confluence, and was removed with a total pancreatectomy, resection of the portal vein and reconstruction with the internal jugular vein. Histological examination confirmed the R-0 resection of the primary SPT, although a vascular invasion was demonstrated. The postoperative course was uneventful, but 32 mo after surgery the patient experienced diffuse liver metastases. Chemotherapy with different drugs was started. The patient is alive and symptom-free, with stable disease, 75 mo after surgery. Twenty-five patients with invasion of the portal vein and/or of mesenteric vessels were retrieved from the literature, 16 recent patients with tumor relapse after potentially curative resection were also retrieved. The best treatment remains a radical resection whenever possible, even in locally advanced or metastatic disease. The role of chemotherapy, and/or radiotherapy, is still to be defined. | Cosimo Sperti Mattia Berselli Claudio Pasquali Davide Pastorelli Sergio Pedrazzoli | 2008 | World Journal of Gastroenterology2008，14，6： | 44 |
| 2 | Introducing an enhanced recovery after surgery program in colorectal surgery:A single center experience显示文摘AIM:To study the implementation of an enhanced recovery after surgery(ERAS)program at a large University Hospital from'pilot study'to'standard of care'.METHODS:The study was designed as a prospective single centre cohort study.A prospective evaluation of compliance to a protocol based on full application of all ERAS principles,through the progressive steps of its implementation,was performed.Results achieved in the initial pilot study conducted by a dedicated team(n=47)were compared to those achieved in the shared protocol phase(n=143)three years later.Outcomes were length of postoperative hospital stay,readmission rate,compliance to the protocol and morbidity.Primary endpoint was the description of the results and the identification of critical issues of large scale implementation of an ERAS program in colorectal surgery emerged in the experience of a single center.Secondary endpoint was the identification of interventions that have been proven to be effective for facilitating the transition from traditional care pathways to a multimodal management protocol according to ERAS principles in colorectal surgery at a single center.RESULTS:During the initial pilot study(March 2009 to December 2010;47 patients)conducted by a dedicated multidisciplinary team,compliance to the items of ERAS protocol was 93%,with a median length of hospital stay(LOS)of 3 d.Early anastomotic fistulas were observed in 2 cases(4.2%),which required reoperation(Clavien-Dindo gradeⅢb).None of the patients had been discharged before the onset of the complication,which could therefore receive prompt treatment.There were also four(8.5%)minor complications(ClavienDindo gradeⅡ).Thirty days readmission rate was 4%.Perioperative mortality was nil.After implementation of the protocol throughout the Hospital in unselected patients(May 2012 to December 2012;147 patients)compliance was 74%,with a median LOS of 6 d.Early anastomotic fistulas were observed in 11 cases(7.7%),5(3.5%)of which required reoperation(Clavien-Dindo gradeⅢb).Two early anastomotic fistulas were treated by radiologic/endoscopic manoeuvres and 4 were treated conservatively.There were also 36(25.2%)minor complications,21(14.7%)of which were Clavien-Dindo gradeⅡand 15(10.5%)of which were Clavien-Dindo gradeⅠ.Only two patients whose course was adversely affected by the development of an anastomotic leak had been discharged before the onset of the complication itself,requiring readmission.Readmission rate within 30 d was 4%.Perioperative mortality was 1%.CONCLUSION:Our results confirm that introduction of an ERAS protocol for colorectal surgery allows quickerpostoperative recovery and shortens the length of stay compared to historical series. | Stefano Bona Mattia Molteni Riccardo Rosati Ugo Elmore Pietro Bagnoli Roberta Monzani Monica Caravaca Marco Montorsi | 2014 | World Journal of Gastroenterology2014，20，46： | 30 |
| 3 | Human bocavirus in children hospitalized for acute respiratory tract infection in Rome显示文摘Background The role of human bocavirus (HBoV) as a respiratory pathogen has not been fulfilled yet.We aimed to describe clinical and serological characteristics of children with HBoV hospitalized for acute respiratory tract infection and to evaluate whether differences occur between HBoV alone and in co-infection.Methods We retrospectively reviewed data from 60 children (median age of 6.2 months,range 0.6-70.9) hospitalized for acute respiratory symptoms,with HBoV detected from a respiratory sample,using a reverse transcriptase-PCR for 14 respiratory viruses (including respiratory syncytial virus (RSV),influenza virus A and B,human coronavirus OC43,229E,NL-63 and HUK1,adenovirus,rhinovirus,parainfluenza virus 1-3,and human metapneumovirus).Results HBoV was detected alone in 29 (48.3%) patients,while in co-infection with other viruses in 31 patients (51.7%),with a peak between December and January.Among the 60 patients,34 were bronchiolitis,19 wheezing,3 pneumonia,2 upper respiratory tract infection,and 2 whooping cough.Seven children (11.6%) required admission to the paediatric intensive care unit (PICU) for respiratory failure.No differences was observed in age,family history for atopy and/or asthma,clinical presentations,chest X-ray,or laboratory findings in children with HBoV alone vs.multiple viral detection.RSV was the most frequently co-detected virus (61.3%).When compared with HBoV detection alone,the co-detection of RSV and HBoV was associated with male sex (P=0.013),younger age (P=0.01),and lower blood neutrophil count (P=0.032).Conclusions HBoV can be detected alone and in co-infection respiratory samples of children with an acute respiratory tract infection.A cause-effect relationship between HBoV and respiratory infection is not clear,so further studies are needed to clarify this point. | Laura Petrarca Raffaella Nenna Antonella Frassanito Alessandra Pierangeli Greta Di Mattia Carolina Scagnolari Fabio Midulla | 2020 | World Journal of Pediatrics2020，16，3： | 17 |
| 4 | Micro RNAs as lung cancer biomarkers显示文摘Lung cancer is the leading cause of cancer mortality worldwide. Its high mortality is due to the poor prognosis of the disease caused by a late disease presentation, tumor heterogeneities within histological subtypes, and the relatively limited understanding of tumor biology. Importantly, lung cancer histological subgroups respond differently to some chemotherapeutic substances and side effects of some therapies appear to vary between subgroups. Biomarkers able to stratify for the subtype of lung cancer, prognosticate the course of disease, or predict the response to treatment are in high demand. In the last decade, microR NAs(miR NAs), measured in resected tumor samples or in fine needle aspirate samples have emerged as biomarkers for tumor diagnosis, prognosis and prediction of response to treatment, due to the ease of their detection and in their extreme specificity. Moreover, miR NAs present in sputum, in plasma, in serum or in whole blood have increasingly been explored in the last five years as less invasive biomarkers for the early detection of cancers. In this review we cover the increasing amounts of datathat have accumulated in the last ten years on the use of miR NAs as lung cancer biomarkers. | Valerio Del Vescovo Margherita Grasso Mattia Barbareschi Michela A Denti | 2014 | World Journal of Clinical Oncology2014，5，4： | 11 |
| 5 | Human bocavirus: Current knowledge and future challenges显示文摘Human bocavirus(HBoV) is a parvovirus isolated about a decade ago and found worldwide in both respiratory samples, mainly from early life and children of 6-24 mo of age with acute respiratory infection, and in stool samples, from patients with gastroenteritis. Since then, other viruses related to the first HBoV isolate(HBoV 1), namely HBoV 2, HBoV 3 and HBoV 4, have been detected principally in human faeces. HBo Vs are small nonenveloped single-stranded DNA viruses of about 5300 nucleotides, consisting of three open reading frames encoding the first two the non-structural protein 1(NS1) and nuclear phosphoprotein(NP1) and the third the viral capsid proteins 1 and 2(VP1 and VP2). HBoV pathogenicity remains to be fully clarified mainly due to the lack of animal models for the difficulties in replicating the virus in in vitro cell cultures, and the fact that HBo V infection is frequently accompanied by at least another viral and/or bacterial respiratory and/or gastroenteric pathogen infection. Current diagnostic methods to support HBoV detection include polymerase chain reaction, real-time PCR, enzymelinked immunosorbent assay and enzyme immunoassay using recombinant VP2 or virus-like particle capsid proteins, although sequence-independent amplification techniques combined with next-generation sequencing platforms promise rapid and simultaneous detection of the pathogens in the future. This review presents the current knowledge on HBoV genotypes with emphasis on taxonomy, phylogenetic relationship and genomic analysis, biology, epidemiology, pathogenesis and diagnostic methods. The emerging discussion on HBoV s as true pathogen or innocent bystander is also emphasized. | Marcello Guido Maria Rosaria Tumolo Tiziano Verri Alessandro Romano Francesca Serio Mattia De Giorgi Antonella De Donno Francesco Bagordo Antonella Zizza | 2016 | World Journal of Gastroenterology2016，22，39： | 11 |
| 6 | Antegrade scrotal sclerotherapy of internal spermatic veins for varicocele treatment: technique, complications, and results显示文摘精索静脉曲张修理主要与临床的摸得出的精索静脉曲张和反常精液参数在年轻成年病人被显示。精索静脉曲张治疗在精子集中,活动性,形态学,和怀孕率与重要改进被联系。Antegrade 阴囊的 sclerotherapy (驴) 代表了的主要选择之一传统腹股沟或 suprainguinal 外科的结扎。这篇文章为精索静脉曲张治疗考察驴的使用。我们提供我们的方法在驴使用了的过程和礼品的历史的简短概述。另外,我们考察驴的复杂并发症和成功,包括我们对待在最后 17 年的 674 个病人的自己的回顾的数据。此处,我们一步一步地分析了 ASS 技术并且与长后续与一种原来的修改技术描述了我们的结果。在 1997 年 12 月和 2014 年 12 月之间,我们执行了 674 驴。吝啬的起作用的时间是 14 min (范围 9 ~ 50 min ) 。没有重要 intraoperative 复杂并发症被报导。在从过程的 90 天以内,手术后的复杂并发症在外套 49 被记录(7.2%) 病人。没有主要复杂并发症被记录。坚持 / 周期性的精索静脉曲张在 40 被检测(5.9%) 案例。在 32/40 (80%) 案例,病人们显示出外科手术前的等级 III 精索静脉曲张。在有在外科前的一个低精子数字的病人,精子计数从 13 × 改善了;10 6 到 21 ×10 6 ml −1(P <0.001 ) 。在 1 h 的进步能动形式的百分比的中部的价值从 25% ~ 45% 改善了(P <0.001 ) 。正常形式的百分比在过程以后在外科前从 17% 增加了到 35% 1 年(P <0.001 ) 。在 168 个不肥沃的病人的亚群,(31%) 52 在 12-month-minimum 后续生了后代。因此,驴是为有低复发 / 坚持率的精索静脉曲张的有效最小的侵略治疗。 | Alessandro Crestani Gianluca Giannarini Mattia Calandriello Marta Rossanese Mariangela Mancini Giacomo Novara Vincenzo Ficarra | 2016 | Asian Journal of Andrology2016，18，2： | 9 |
| 7 | Hepatic resection beyond barcelona clinic liver cancer indication:When and how显示文摘Hepatocellular carcinoma(HCC)is the main common primary tumour of the liver and it is usually associated with cirrhosis.The barcelona clinic liver cancer(BCLC)classification has been approved as guidance for HCC treatment algorithms by the European Association for the Study of Liver and the American Association for the Study of Liver Disease.According to this algorithm,hepatic resection should be performed only in patients with small single tumours of 2-3 cm without signs of portal hypertension(PHT)or hyperbilirubinemia.BCLC classification has been criticised and many studies have shown that multiple tumors and large tumors,as wide as those with macrovascular infiltration and PHT,could benefit from liver resection.Consequently,treatment guidelines should be revised and patients with intermediate/advanced stage HCC,when technically resectable,should receive the opportunity to be treated with radical surgical treatment.Nevertheless,the surgical treatment of HCC on cirrhosis is complex:The goal to be oncologically radical has always to be balanced with the necessity to minimize organ damage.The aim of this review was to analyze when and how liver resection could be indicated beyond BCLC indication.In particular,the role of multidisciplinary approach to assure a proper indication,of the intraoperative ultrasound for intraoperative restaging and resection guidance and of laparoscopy to minimize surgical trauma have been enhanced. | Mattia Garancini Enrico Pinotti Stefano Nespoli Fabrizio Romano Luca Gianotti Vittorio Giardini | 2016 | World Journal of Hepatology2016，8，11： | 8 |
| 8 | Pharmacogenetics of the systemic treatment in advanced hepatocellular carcinoma显示文摘Hepatocellular carcinoma (HCC) accounts for the majority of primary liver cancers. To date, most patients with HCC are diagnosed at an advanced tumor stage, excluding them from potentially curative therapies (i.e., resection, liver transplantation, percutaneous ablation). Treatments with palliative intent include chemoembolization and systemic therapy. Among systemic treatments, the small-molecule multikinase inhibitor sorafenib has been the only systemic treatment available for advanced HCC over 10 years. More recently, other smallmolecule multikinase inhibitors (e.g., regorafenib, lenvatinib, cabozantinib) have been approved for HCC treatment. The promising immune checkpoint inhibitors (e.g., nivolumab, pembrolizumab) are still under investigation in Europe while in the US nivolumab has already been approved by FDA in sorafenib refractory or resistant patients. Other molecules, such as the selective CDK4/6inhibitors (e.g., palbociclib, ribociclib), are in earlier stages of clinical development, and the c- MET inhibitor tivantinib did not show positive results in a phase III study. However, even if the introduction of targeted agents has led to great advances in patient response and survival with an acceptable toxicity profile, a remarkable inter-individual heterogeneity in therapy outcome persists and constitutes a significant problem in disease management. Thus, the identification of biomarkers that predict which patients will benefit from a specific intervention could significantly affect decision-making and therapy planning. Germ-line variants have been suggested to play an important role in determining outcomes of HCC systemic therapy in terms of both toxicity and treatment efficacy. Particularly, a number of studies have focused on the role of genetic polymorphisms impacting the drug metabolic pathway and membrane translocation as well as the drug mechanism of action as predictive/prognostic markers of HCC treatment. The aim of this review is to summarize and critically discuss the pharmacogenetic literature evidences, with particular attention to sorafenib and regorafenib, which have been used longer than the others in HCC treatment. | Elena De Mattia Erika Cecchin Michela Guardascione Luisa Foltran Tania Di Raimo Francesco Angelini Mario D’Andrea Giuseppe Toffoli | 2019 | World Journal of Gastroenterology2019，25，29： | 8 |
| 9 | Size Distributions of Aerosol Sulfates and Nitrates in Beijing during the 2008 Olympic Games: Impacts of Pollution Control Measures and Regional Transport显示文摘为 2008 个奥运会,激烈的控制措施在二氧化物(SO2 ) ,氮氧化物(没有 x ) 和处理穷人的问题的另外的污染物质通风的硫的工业、城市的排出物上被实现在北京的质量。在微粒硫酸盐和硝酸盐集中以及他们的尺寸分布上调查 SO2 和没有 x 减小的效果,分离尺寸的喷雾器样品在照原尺寸的控制前后在北京在城市、顺风的农村地点用微孔的一致存款 impactors (MOUDI ) 被收集。在采样时期期间,在城市、农村的地点的好粒子(PM1.8 ) 的集体集中分别地是 94.0 和 85.9 g m3。超过 90% 硫酸盐和 60% 硝酸盐形成了同样好的粒子。得益于有益的气象学的条件和来源控制,硫酸盐在奥林匹克期间在相当低的集中并且首先在冷凝作用模式被观察。控制措施的效果独立被分析让北风和南方的 air-mass-dominated 天说明任何偏爱。在控制措施被实现以后,当在北方的空气群众占优势时,下午,硫酸盐,和硝酸盐显著地被减少,与在更大的粒子的减小的一个更高的百分比。在控制措施被实现以后,微滴模式粒子,在控制被实现以前,统治了硫酸盐和硝酸盐,显著地在集体集中被减少。但当弄脏的南方的空气群众占优势时,本地来源控制在北京测量,有效地没从北方中国平原由于庞大的地区性的贡献减少周围的硫酸盐集中。 | WANG Xinfeng WANG Tao Ravi Kant PATHAK Mattias HALLQUIST GAO Xiaomeit NIE Wei XUE Likun GAO Jian GAO Rui ZHANG Qingzhu WANG Wenxing WANG Shulan CHAI Fahe CHEN Yizhen | 2013 | Advances in Atmospheric Sciences2013，30，2： | 8 |
| 10 | Photochemical smog in China:scientific challenges and implications for air-quality policies显示文摘INTRODUCTION Severe air-pollution events in many parts of China pose a major threat to health and ecosystems[1].China’s air pollution is concentrated to economically developed areas,such as Beijing–Tianjin–Hebei(BTH)and Pearl–River–Delta(PRD)[2,3].The situation has received considerable attention in | Mattias Hallquist John Munthe Min Hu Tao Wang Chak K Chan Jian Gao Johan Boman Song Guo Asa M Hallquist Johan Mellqvist Jana Moldanova Ravi K Pathak Jan BC Pettersson Hakan Pleijel David Simpson Marie Thynell | 2016 | National Science Review2016，3，4： | 8 |
| 11 | 气体吸附法进行孔径分析进展--密度函数理论(DFT)及蒙特卡洛法(MC)的应用显示文摘 气体吸附法是获得多孔材料全面表征的极好方法,它可以反映比表面、孔分布和孔隙度等方面的信息,但是,这需要对吸附过程有一个详细的了解,包括在多孔材料上流体的吸附和相变化及其对吸附等温线的影响,这是表面分析和孔分析的基础.孔宽、孔形及有效的吸附能是测定孔填充过程的因子.如果是所谓微孔(按照IUPAC分类,孔宽<2 nm)孔填充是一个连续的过程;而如果是介孔(中孔,孔宽在2~50 nm之间),孔填充则是气体在孔内的凝聚过程,它表现为一级气-液相转移.…… | 杨正红 Dr Mattias Thommes | 2005 | 中国粉体技术2005，11，z1： | 8 |
| 12 | Kounis syndrome:a clinical entity penetrating from pediatrics to geriatrics显示文摘1 Introduction Kounis syndrome constitutes a coronary hypersensitivity disorder defined by the association of an anaphylactoid,anaphylactic,allergic or hypersensitivity reaction with an acute coronary syndrome,in a physiopathological context involving various interrelated and interacting inflammatory cells,such as mast-cells,eosinophils and platelets.[1,2]Similar entities to Kounis syndrome might involve cerebral and mesenteric arteries. | Mattia Giovannini Ioanna Koniari Francesca Mori Silvia Ricci Luciano De Simone Silvia Favilli Sandra Trapani Giuseppe Indolfi Nicholas George Kounis Elio Novembre | 2020 | Journal of Geriatric Cardiology2020，17，5： | 7 |
| 13 | Stem cells sources for intervertebral disc regeneration显示文摘Intervertebral disc regeneration field is rapidly growing since disc disorders represent a major health problem in industrialized countries with very few possible treatments.Indeed, current available therapies are symptomatic, and surgical procedures consist in disc removal and spinal fusion, which is not immune to regardable concerns about possible comorbidities, cost-effectiveness, secondary risks and long-lasting outcomes. This review paper aims to share recent advances in stem cell therapy for the treatment of intervertebral disc degeneration. In literature the potential use of different adult stem cells for intervertebral disc regeneration has already been reported. Bone marrow mesenchymal stromal/stem cells, adipose tissue derived stem cells, synovial stem cells, muscle-derived stem cells, olfactory neural stem cells, induced pluripotent stem cells, hematopoietic stem cells, disc stem cells, and embryonic stem cells have been studied for this purpose either in vitro or in vivo. Moreover, several engineered carriers(e.g., hydrogels), characterized by full biocompatibility and prompt biodegradation, have been designed and combined with different stem cell types in order to optimize the local and controlled delivery of cellular substrates in situ. The paper overviews the literature discussing the current status of our knowledge of the different stem cells types used as a cell-based therapy for disc regeneration. | Gianluca Vadalà Fabrizio Russo Luca Ambrosio Mattia Loppini Vincenzo Denaro | 2016 | World Journal of Stem Cells2016，8，5： | 7 |
| 14 | 气体吸附法进行孔径分析进展——密度函数理论(DFT)及蒙特卡洛法(MC)的应用显示文摘1.前言 气体吸附法是获得多孔材料全面表征的极好方法,它可以反映比表面、孔分布和孔隙度等方面的信息,但是,这需要对吸附过程有一个详细的了解,包括在多孔材料上流体的吸附和相变化及其对吸附等温线的影响,这是表面分析和孔分析的基础。 | 杨正红 Mattias Thommes | 2009 | 中国粉体工业2009，，6： | 6 |
| 15 | 持续性心房颤动山羊模型的心房单极电极标测显示文摘在持续性心房颤动 (简称房颤 )山羊模型上 ,研究心房不同部位房颤周长 (AFCL)及单极心外膜电图的差异 ,并利用激动标测图分析房颤时的蝉联现象。将 83个电极分别缝合于 7只山羊的左心房游离壁、左心耳、Bachmann束 (BB)、右心耳游离壁的心外膜。利用自动房颤刺激器维持房颤 ,待房颤持续 4周后 ,取 16s的心外膜电图作分析。利用单极电图标记局部激动时间 ,并根据单极电图的形态特点将其标记为正常电位 (单电位和短的双电位 )及异常电位 (长的双电位、三电位及碎裂电位 )。根据左心房局部激动时间重建等电位激动标测图 ,如超过 7次连续心房搏动经过相同路径 ,则定义为蝉联片段。在左心房、左心耳、BB、右心耳及右心房的AFCL分别为 94 .9± 4 .6 ,95 .7±4 .4 ,10 5 .7± 6 .5 (与其他部位比较 ,P <0 .0 5 ) ,99.2± 8.0及 98.5± 6 .3ms ;异常电位的百分比分别为 2 0 .9%± 6 .3% ,2 7.8%± 11.8% ,5 7.4 %± 7.8% (与其他部位比较 ,P <0 .0 1) ,18.6 %± 9.7%和 19.4 %± 3.9%。在所记录的 16s房颤过程中 ,左心房共有 70 5次心搏 ,其中 6 8次 (9.6 % )属于蝉联片段。结论 :在山羊持续房颤模型 ,BB的AFCL最长 ,异常电位的发生率最高 ,提示BB在房颤的维持上起重要作用。 | 单兆亮 王玉堂 时向民 Mattias Duytschaever Maurits A.Allessie | 2002 | 中国心脏起搏与心电生理杂志2002，16，3： | 6 |
| 16 | Gut-liver axis signaling in portal hypertension显示文摘Portal hypertension(PHT)in advanced chronic liver disease(ACLD)results from increased intrahepatic resistance caused by pathologic changes of liver tissue composition(structural component)and intrahepatic vasoconstriction(functional component).PHT is an important driver of hepatic decompensation such as development of ascites or variceal bleeding.Dysbiosis and an impaired intestinal barrier in ACLD facilitate translocation of bacteria and pathogen-associated molecular patterns(PAMPs)that promote disease progression via immune system activation with subsequent induction of proinflammatory and profibrogenic pathways.Congestive portal venous blood flow represents a critical pathophysiological mechanism linking PHT to increased intestinal permeability:The intestinal barrier function is affected by impaired microcirculation,neoangiogenesis,and abnormal vascular and mucosal permeability.The close bidirectional relationship between the gut and the liver has been termed“gut-liver axis”.Treatment strategies targeting the gut-liver axis by modulation of microbiota composition and function,intestinal barrier integrity,as well as amelioration of liver fibrosis and PHT are supposed to exert beneficial effects.The activation of the farnesoid X receptor in the liver and the gut was associated with beneficial effects in animal experiments,however,further studies regarding efficacy and safety of pharmacological FXR modulation in patients with ACLD are needed.In this review,we summarize the clinical impact of PHT on the course of liver disease,discuss the underlying pathophysiological link of PHT to gut-liver axis signaling,and provide insight into molecular mechanisms that may represent novel therapeutic targets. | Benedikt Simbrunner Mattias Mandorfer Michael Trauner Thomas Reiberger | 2019 | World Journal of Gastroenterology2019，25，39： | 6 |
| 17 | DoE实验设计开发23价肺炎多糖疫苗层析纯化平台工艺显示文摘目的:应用Do E实验设计,建立一种新型23价肺炎多糖疫苗层析纯化工艺平台。方法:将23F型肺炎粗糖样品作为开发样品溶解并预处理后,采用Do E设计开发基于Capto Adhere的纯化工艺。色谱纯化过程采用流穿模式,通过Do E分析获得工艺参数,在该条件下将多糖和其他杂质分离,并作为纯化平台应用于其他血清型多糖。根据《欧洲药典》7.0的方法和标准,对样品的各项指标进行检测。结果:23F型多糖在柱上流穿,多糖与蛋白和核酸完全分离,应用此工艺平台至其他血清型多糖上,纯化的多糖样品各项指标也均符合《欧洲药典》7.0的标准。结论:通过Do E实验设计快速建立了一种新型肺炎多糖疫苗层析纯化工艺平台,缩短生产时间,提高产品质量,代替传统的有毒苯酚抽提工艺,可用于23价肺炎多糖纯化和23价肺炎多糖疫苗生产。 | 季宇 Mattias Ahnfelt 贾国栋 | 2016 | 中国新药杂志2016，25，10： | 5 |
| 18 | Tectonically asymmetric Earth:From net rotation to polarized westward drift of the lithosphere显示文摘The possibility of a net rotation of the lithosphere with respect to the mantle is generally overlooked since it depends on the adopted mantle reference frames,which are arbitrary.We review the geological and geophysical signatures of plate boundaries,and show that they are markedly asymmetric worldwide.Then we compare available reference frames of plate motions relative to the mantle and discuss which is at best able to fit global tectonic data.Different assumptions about the depths of hotspot sources(below or within the asthenosphere,which decouples the lithosphere from the deep mantle) predict different rates of net rotation of the lithosphere relative to the mantle.The widely used no-net-rotation(NNR) reference frame,and low(<0.2°-0.4° /Ma) net rotation rates(deep hotspots source) predict an average net rotation in which some plates move eastward relative to the mantle(e.g.,Nazca).With fast(>1° /Ma) net rotation(shallow hotspots source),all plates,albeit at different velocity,move westerly along a curved trajectory,with a tectonic equator tilted about 30° relative to the geographic equator.This is consistent with the observed global tectonic asymmetries. | Carlo Doglioni Eugenio Carminati Mattia Crespi Marco Cuffaro Mattia Penati Federica Riguzzi | 2015 | Geoscience Frontiers2015，6，3： | 5 |
| 19 | Towards a knowledge base to support global change policy goals显示文摘In 2015,it was adopted the 2030 Agenda for Sustainable Development to end poverty,protect the planet and ensure that all people enjoy peace and prosperity.The year after,17 Sustainable Development Goals(SDGs)officially came into force.In 2015,GEO(Group on Earth Observation)declared to support the implementation of SDGs.The GEO Global Earth Observation System of Systems(GEOSS)required a change of paradigm,moving from a data-centric approach to a more knowledge-driven one.To this end,the GEO System-of-Systems(SoS)framework may refer to the well-known Data-Information-Knowledge-Wisdom(DIKW)paradigm.In the context of an Earth Observation(EO)SoS,a set of main elements are recognized as connecting links for generating knowledge from EO and non-EO data–e.g.social and economic datasets.These elements are:Essential Variables(EVs),Indicators and Indexes,Goals and Targets.Their generation and use requires the development of a SoS KB whose management process has evolved the GEOSS Software Ecosystem into a GEOSS Social Ecosystem.This includes:collect,formalize,publish,access,use,and update knowledge.ConnectinGEO project analysed the knowledge necessary to recognize,formalize,access,and use EVs.The analysis recognized GEOSS gaps providing recommendations on supporting global decision-making within and across different domains. | Stefano Nativi Mattia Santoro Gregory Giuliani Paolo Mazzetti | 2020 | International Journal of Digital Earth2020，13，2： | 5 |
| 20 | Exploring the depths of the global earth observation system of systems显示文摘This paper explores for the first time the contents,structure and relationships across institutions and disciplines of a global Big Earth Data cyber-infrastructure:the Global Earth Observation System of System(GEOSS).The analysis builds on 1.8 million metadata records harvested in GEOSS.Because this set includes almost all the major large data collections in GEOSS,the analysis represents more than 80%of all the data made available through this global system.We explore two major aspects:the collaborative networks and the thematic coverage in GEOSS.The first connects the contributing organisations through the more than 200,000 keywords used in the systems,and then explores who is citing whom,a proxy for of institutional thickness.The thematic coverage is analysed through neural network algorithms,first on the keywords,and then on the corpus of 653 million lemmatised lower case words built from the titles and abstracts of all 1.8 million metadata records.The findings not only give a good overview of the GEOSS data universe,but offer immediate priorities on how to increase the usability of GEOSS through improved data management,and the opportunity to augment the metadata with high level concept that synthetise well the contents of the data-set. | Max Craglia Jiri Hradec Stefano Nativi Mattia Santoro | 2017 | Big Earth Data2017，1，1： | 5 |
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