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| 1 | A Multifunctional Lentiviral-Based Gene Knockdown with Concurrent Rescue that Controls for Off-Target Effects of RNAi显示文摘The efficient, stable delivery of siRNA into cells, and the appropriate controls for non-specific off-target effects of siRNA are major limitations to functional studies using siRNA technology. To overcome these drawbacks, we have developed a single lentiviral vector that can concurrently deplete endogenous gene expression while expressing an epitope-tagged siRNA-resistant target gene in the same cell. To demonstrate the functional utility of this system, we performed RNAi-depleted α-actinin-1 (α-ACTN1) expression in human T cells. α-ACTN1 RNAi resulted in inhibited chemotaxis to SDF-1α, but it can be completely rescued by concurrent expression of RNAi-resistant α-ACTN1 (rr-α-ACTN1) in the same cell. The presence of a GFP tag on rr-α-ACTN1 allowed for detection of appropriate subcellular localization of rr-α-ACTN1. This system provides not only an internal control for RNAi off-target effects, but also the potential tool for rapid structure-function analyses and gene therapy. | Yunfeng Feng Linghu Nie Meghna Das Thakur Qin Su Zhenfen Chi Yongliang Zhao Gregory D. Longmore | 2010 | Genomics, Proteomics & Bioinformatics2010，8，4： | 2 |
| 2 | Caesium-137 profiles in the sediments of a partial-meromictic lake on Great Sandy Island (Fraser Island),Queensland,Australia显示文摘 | LONGMORE M E O'LEARY B M ROSE C W | 1983 | Hydrobiologia1983，103，： | 1 |
| 3 | Malnutrition in hospital outpatients and inpatients: prevalence concurrent validity and ease of use of the ‘malnutrition universal screening tool' ( ‘MUST' ) for adults 显示文摘 | Stratton RJ Hackston A Longmore D | 2004 | Br J Nutr2004，92，5： | 1 |
| 4 | Dimensional accuracy of magnetic resonance in studies of the heart显示文摘 | Longmore DB Klipstein RH Underwood SR | 1985 | Lancet1985，1，8442： | 1 |
| 5 | Political movements of people with disabilities:The league of the physically handicapped,1935-38显示文摘 | Longmore PK Goldberger D | 1997 | Disability Studies Quarterly1997，17，2： | 1 |
| 6 | MRI studies of atherosclerotic vascular disease: structural evaluation and physiological measu- rements显示文摘 | Mohiaddin RH Longmore DB | 1989 | Br Med Bull1989，45，： | 1 |
| 7 | Malnutrition in hospital out- patients and inpatients:prevalence concurrent validity and ease of use of the 'Malnutrition universal screening tool' ('MUST') for adults显示文摘 | Stratton ILl Hackston A Longmore D | 2004 | Br J Nutr2004，92，5： | 1 |
| 8 | Prolonged prostate-specific antigen response in flutamide withdrawal syndrome despite disease progression显示文摘 | Longmore L Foley JP Rozanski TA | 1998 | South Med J1998，91，： | 1 |
| 9 | Malnutrition in hospital outpa- tients and inpatients: prevalence, concurrent validity and ease of use of the malnutrition universal screening tool (MUST) for adults 显示文摘 | Stratton FJ Hackston A Longmore D | 2004 | British Journal of Nutrition2004，92，： | 1 |
| 10 | Belt torque loss in a steel V-beh continuously variable transmission 显示文摘 | MICKLEM J D LONGMORE D K BURROWS C R | 1994 | Journal of Automobile Engineering1994，3208，： | 1 |
| 11 | The erythro-poietin receptor: its role in hernatopoiesis and myeloproliferative diseases显示文摘 | Longmore G D Watowich S S Hilton D J | 1993 | J CellBiol1993，123，： | 1 |
| 12 | Mod- eling of the Steel Pushing V- belt Continuously Variable Transmission 显示文摘 | Micklem J M Longmore D K Burrows C R | 1994 | Journal of Mechanical Engineering Science Part C1994，208，1： | 1 |
| 13 | Malnutrition in hospital outpatients and inpatients:prevalence,concurrent validity and ease of use of the 'malnutrition universal screening tool'('MUST') for adults显示文摘 | Hackston A Longmore D | 2004 | Br J Nutr2004，92，： | 1 |
| 14 | Human resource management prac-tices in a medical complex in the eastern cape,south africa: as-sessing their impact on the retention of doctors显示文摘 | Longmore B Ronnie L | 2014 | S Afr MedJ2014，104，5： | 1 |
| 15 | Benthic Nutrient Recycling in Port Phillip Bay,Australia显示文摘 | Berelson W M Heggie D Longmore A | 1998 | Estuarine Coastal and Shelf Science1998，46，： | 1 |
| 16 | An insurance contracts act for a new century? 显示文摘 | LONGMORE A | 2001 | Lloyd'' s Maritime and Commercial Law Quarterly2001，，3： | 1 |
| 17 | Contractile 5-HT1B receptors in human cerebral arteries: pharmacological characterization and localization with immunocytochemistry 显示文摘 | NILSSON T LONGMORE J SHAW D | 1999 | Br J Pharmaeol1999，128，6： | 1 |
| 18 | The LIM protein Ajuba influences interleukin-1-induced NF-kappaB activation by affecting the assembly and activity of the protein kinase Czeta/p62/TRAF6 signaling complex显示文摘 | Feng Y Longmore GD | 2005 | Mol Cell Biol2005，25，10： | 1 |
| 19 | Structure, function, and activation of the erythropoietin receptor 显示文摘 | Youssoufian H Longmore G Neumann D | 1993 | Blood1993，81，： | 1 |
| 20 | Dietary sandalwood seed oil modifies fatty acid composition of mouse adipose tissue,brain,and liver显示文摘 | Liu Y Longmore R B | 1997 | Lipid1997，32，9： | 1 |
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