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| 1 | Cathepsin L upregulation-induced EMT phenotype is associated with the acquisition of cisplatin or paclitaxel resistance in A549 cells显示文摘组织蛋白酶 L (CTSL ) , lysosomal 酸半胱氨酸朊酶,被知道在肿瘤起重要作用转移和化疗抵抗。在这研究,我们调查了在人的肺癌症 cells.Methods 由 CTSL 位于 chemoresistance 的规定下面的分子的机制:人的肺癌症 A549 房间, A549/PTX (paclitaxel 抵抗) 房间和 A549/DDP (cisplatin 抵抗) 房间被测试。到 cisplatin 或 paclitaxel 的抵抗用 MTT 和殖民地形成试金被检测。肌动朊改变与 FITC-Phalloidin 被观察荧光灯的染色或 immunofluorescence。愈合创伤的试金或 Transwell 试金被用来估计迁居或侵略能力。CTSL 和上皮、间充质的标记的表示与西方的弄污和 immunofluorescence 被分析。联系 EMT 的抄写因素的表示与西方的弄污或 q-PCR 被测量。BALB/c 裸体老鼠与 A549 房间 overexpressing CTSL 皮下地被植入,并且老鼠是管理 paclitaxel (10, 15 mg/kg, ip ) 为 5 times.Results 的每 3 d:Cisplatin 或 paclitaxel 治疗(10-80 ng/mL ) 在 A549 房间导致了 CTSL 表示。CTSL 层次比在 A549 房间在 A549/PTX 和 A549/DDP 房间是高得多的。CTSL 的 Silencing 在 A549/DDP 和 A549/TAX 房间颠倒了 chemoresistance,而 CTSL 的 overexpression 稀释了 A549 房间的敏感到 cisplatin 或 paclitaxel。而且, A549/DDP 和 A549/TAX 房间经历了词法并且有增加的房间侵略和迁居能力的细胞骨架变化,由上皮的标记( E-cadherin 和 cytokeratin-18 )的减少的表示伴随了并且增加间充质的标记( N-cadherin 和 vimentin )的表示,以及 联系EMT 的抄写因素的 upregulation 蜗牛,蛞蝓, ZEB1 和 ZEB2 。CTSL 的 Silencing 在 A549/DDP 和 A549/TAX 房间颠倒了 EMT;相反, CTSL 的 overexpression 在 A549 房间导致了 EMT。在异种皮移植裸体人鼠标模型,与 A549 房间 overexpressing CTSL 植入的鼠标展出了显著地减少的敏感到 paclitaxel 处理,并且在肿瘤 tissues.Conclusion 增加了联系 EMT 的蛋白质和抄写因素的表示:Cisplatin 和 paclitaxel 抵抗在 A549 房间与 CTSL 导致 upregulation 的 EMT 被联系。因此,调停 CTSL 的 EMT 可以作为一个目标被利用对肺癌症和恶意的另外的类型提高 cisplatin 或 paclitaxel 的功效。 | Mei-ling HAN Yi-fa n ZHAO Cai-hong TAN Ya-jie XIONG Wen-juan WANG Feng WU Yao FEI Long WANG Zhong-qin LIANG | 2016 | Acta Pharmacologica Sinica2016，37，12： | 15 |
| 2 | Th1 cytokines promote T-cell binding to antigen-presenting cells via enhanced hyaluronan production and accumulation at the immune synapse显示文摘Hyaluronan(HA)production by dendritic cells(DCs)is known to promote antigen presentation and to augment T-cell activation and proliferation.We hypothesized that pericellular HA can function as intercellular‘glue’directly mediating T cell–DC binding.Using primary human cells,we observed HA-dependent binding between T cells and DCs,which was abrogated upon pre-treatment of the DCs with 4-methylumbelliferone(4-MU),an agent which blocks HA synthesis.Furthermore,T cells regulate HA production by DCs via T cell-derived cytokines in a T helper(Th)subset-specific manner,as demonstrated by the observation that cell-culture supernatants from Th1 but not Th2 clones promote HA production.Similar effects were seen upon the addition of exogenous Th1 cytokines,IL-2,interferon c(IFN-c)and tumor necrosis factor a(TNF-a).The critical factors which determined the extent of DC–T cell binding in this system were the nature of the pre-treatment the DCs received and their capacity to synthesize HA,as T-cell clones which were pre-treated with monensin,added to block cytokine secretion,bound equivalently irrespective of their Th subset.These data support the existence of a feedforward loop wherein T-cell cytokines influence DC production of HA,which in turn affects the extent of DC–T cell binding.We also document the presence of focal deposits of HA at the immune synapse between T-cells and APC and on dendritic processes thought to be important in antigen presentation.These data point to a pivotal role for HA in DC–T cell interactions at the IS. | Paul L Bollyky Stephen P Evanko Rebecca P Wu Susan Potter-Perigo S Alice Long Brian Kinsella Helena Reijonen Kelly Guebtner Brandon Teng Christina K Chan Kathy R Braun John A Gebe Gerald T Nepom Thomas N Wight | 2010 | Cellular & Molecular Immunology2010，7，3： | 3 |
| 3 | The empirical mode decomposition and the Hilbert spectrum for nonlinear and non-stationary time series analysis显示文摘 | Huang N E Shen Z Long S R | | 0，，： | 3 |
| 4 | The empirical mode decomposition and the Hilbert spectrum for nonlinear and non-stationary time series analysis显示文摘 | Huang N E Shen Z Long S R | | 0，，1971： | 2 |
| 5 | The empirical mode decomposition and the Hilbert spectrum for nonlinear and nonstationary time series analysis显示文摘 | Huang N E Shen Z Long S R | | 0，，1971： | 2 |
| 6 | The effect of early to mid-gestational nutrient restrietion on female offspring fertility and hypothalamic-pituitary-adrenal axis response to stress 显示文摘 | Long N M Nijland M J Nathanielsz P W | 2010 | J Anim Sci2010，88，6： | 1 |
| 7 | Kuhn Algorithm: Ullla- convenient Solver to Complex Polynomial and Transcendental Equations without Initial Value Selection 显示文摘 | Yunliang Long Edward K N Yung | 2002 | International Journal of RF and Microwave Computer-Aided Engineering2002，12，6： | 1 |
| 8 | A confidence limit for theempirical mode decomposition and the Hilbert spectralanalysis显示文摘 | Huang N E Wu M C Long S R | 2003 | Proceedings of the Royal Society London:Series A2003，31，： | 1 |
| 9 | Effects of rising CO2concentration on expression of photosynthrtic protein显示文摘 | Webber A N Nie G Y Long S P | 1994 | Photosynth Res1994，39，： | 1 |
| 10 | A Confidence Limit for the Empirical Mode Decomposition and Hilbert Spectral Analysis显示文摘 | Huang N E Wu M L Long S R | 2003 | Proceedings of the Royal Society of London2003，459，： | 1 |
| 11 | The empirical mode decomposition and the Hilbert spectrum for nonlinear and nonstationary time series analysis 显示文摘 | Huang N E Zheng Shen Long S R | 1998 | Proceedings of the Royal Society A : Mathematical Physical and Engineering Sciences1998，454，1971： | 1 |
| 12 | The empirical mode decomposition and the Hilbert spectrum for nonlinear and non-stationary time series analysis 显示文摘 | Huang N E Shen Z Long S al | 1998 | Proceedings of the Royal Society of London1998，454，： | 1 |
| 13 | The empirical mode decomposition and the Hilbert spectrum for nonlinear and non-stationary time series analysis 显示文摘 | Huang N E Shen Z Long S R | 1998 | Proceedings Of The Royal Society of London Series A-Mathematical Physical And Engineering Sciences1998，454，： | 1 |
| 14 | The empirical modedecomposition and the Hilbert spectrum for nonlinear andnon-stationary time series analysis显示文摘 | HUANG N E SHEN Z LONG S R | 1998 | Proceedings of theRoyal Society of London1998，454，1： | 1 |
| 15 | Robotic-assisted sacrocol- popexy: technique and learning curve显示文摘 | Akl M N Long J B Giles D L | 2009 | Surgical Endoscopy2009，23，10： | 1 |
| 16 | Investigation of factors af- fecting the diffusion of mobile telephone services: An empirical analysis for Vietnam显示文摘 | Hwang J Cho Y Long N V | 2009 | Telecommunications Policy2009，33，： | 1 |
| 17 | The empirical mode decomposition and the Hilbert spectrum for non- linear and non- stationary time series analysis显示文摘 | Huang N E Shen Z Long S R | 1998 | Proceedings of the Royal Society of London Series A: Mathematical Physical and Engineering Sciences1998，454，： | 1 |
| 18 | Trade,Wage Gapsand Specific Human Capital Accumulation显示文摘 | Long N V Riezman R Soubeyran A | 2007 | Review ofInternational Economics2007，15，6： | 1 |
| 19 | A confidence limit for the empirical mode decomposition and Hilbert spectral analysis 显示文摘 | Huang N E Wu M L C Long S R | 2003 | Proceedings Royal of Society A2003，459，： | 1 |
| 20 | The Os-AKT1 Channel Is Critical for K Uptake in Rice Roots and Is Modulated by the Rice CBL1-CIPK23 Complex 显示文摘 | LI J LONG Y QI G N | 2014 | Plant Cell2014，26，8： | 1 |
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