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| 1 | Variations of pore water sulfate gradients in sediments as indicator for underlying gas hydrate in Shenhu Area, the South China Sea显示文摘Shenhu Area is one of the most promising areas for gas hydrate exploration in the northern South China Sea (SCS). Pore water sulfate gradient, sulfate-methane interface (SMI) depth, and sulfate flux were analyzed at 53 sites in this area. SO42 gradient ranges between 0.33 and 4.43 mmol L-1 m-1 . SMI depths are from 7.7 to 87.9mbsf. Sulfate flux varies between 2.0 and 26.9 mmol m-2 yr-1 , with a mean of 11.7 mmol m-2 yr-1 . Correlation coefficient between SMI depth and methane flux for the 53 sites is 0.80, implying that methane flux regulates the rate of anaerobic methane oxidation (AMO), SMI depth, and sulfate flux. Twelve anomalous fields with high methane flux and steep sulfate gradients were recognized. Bottom simulating reflector (BSR) is distributed mainly in areas where SMI depth is less than 50 mbsf or places with sulfate flux larger than 3.5 mmol m-2 yr-1 . It is suggested that the Baiyun Sag and the Southern Uplift are potential areas for gas hydrate exploration. | WU LuShan YANG ShengXiong LIANG JinQiang SU Xin FU ShaoYing SHA ZhiBin YANG Tao | 2013 | Science China Earth Sciences2013，56，4： | 24 |
| 2 | Types,Characteristics and Significances of Migrating Pathways of Gas-bearing Fluids in the Shenhu Area,Northern Continental Slope of the South China Sea显示文摘The first marine gas hydrate expedition in China has been conducted by Guangzhou Marine Geological Survey in the Shenhu Area, northern continental slope of the South China Sea. Previous study has analyzed the P-T conditions, geophysical anomalies and saturation calculations of these gas hydrates, but has not documented in detail the migration of gas-bearing fluids in the study area. Based on the interpretations of 2D/3D seismic data, this work identified two types of migration pathways for gas-bearing fluids in the Shenhu area, i.e., vertical and lateral pathways. The vertical pathways(largescale faults, gas chimneys and mud diapirs) presented as steep seismic reflection anomalies, which could be traced downward to the Eocene source rocks and may penetrate into the Late Miocene strata. The deeper gases/fluids might be allowed migrating into the shallower strata through these vertical conduits. However, the distributions showed distinct differences between these pathways. Large-scale faults developed only in the north and northeast of the Shenhu area, while in the drilling area gas chimneys were the sole vertical migration pathways. Since the Pliocene, normal faults, detachment faults and favorable sediments have constituted the lateral pathways in the Shenhu gas hydrate drilling area. Although these lateral pathways were connected with gas chimneys, they exerted different effects on hydrate formation and accumulation. Gas-bearing fluids migrated upward along gas chimneys might further migrate laterally because of the normal faults, thereby enlarging the range of the chimneys. Linking gas chimneys with the seafloor, the detachment faults might act as conduits for escaping gases/fluids. Re-deposited sediments developed at the early stage of the Quaternary were located within the gas hydrate stability zone, so hydrates would be enriched in these favorable sediments. Compared with the migration pathways(large-scale faults and mud diapirs) in the LW3-1 deep-sea oil/gas field, the migration efficiency of the vertical pathways(composed of gas chimneys) in the gas hydrate drilling area might be relatively low. Description and qualitative discrimination of migration pathways in the Shenhu gas hydrate drilling area are helpful to further understand the relationship between good-quality deep source rocks and shallow, mainly biogenicallyproduced, hydrates. As the main source rocks of the Baiyun sag, lacustrine mudstones in the Wenchang and Enping Formations may provide thermogenic methane. Gas chimneys with relatively low migration efficiency created the vertical pathways. Caused by the Dongsha tectonic movement, the release of overpressured fluids might reduce the vertical migration rates of the thermogenic methane. The thick bathyal/abyssal fine-grained sediments since the Late Miocene provided migration media with low permeability. These preconditions may cause carbon isotopic fractionation ofthermogenic methane during long-distance vertical migrations. Therefore, although geochemical analyses indicate that the methane forming gas hydrate in the Shenhu area was mainly produced biogenically, or was mixed methane primarily of microbial origin, thermogenic methane still contribute significantly. | SU Ming SHA Zhibin ZHANG Cuimei WANG Hongbin WU Nengyou YANG Rui LIANG Jinqiang QIAO Shaohua CONG Xiaorong LIU Jie | 2017 | Acta Geologica Sinica(English Edition)2017，91，1： | 16 |
| 3 | A new prognostic histopathologic classification of nasopharyngeal carcinoma显示文摘Background:The current World Health Organization(WHO) classification of nasopharyngeal carcinoma(NPC) con?veys little prognostic information.This study aimed to propose an NPC histopathologic classification that can poten?tially be used to predict prognosis and treatment response.Methods:We initially developed a histopathologic classification based on the morphologic traits and cell differentia?tion of tumors of 2716 NPC patients who were identified at Sun Yat?sen University Cancer Center(SYSUCC)(training cohort).Then,the proposed classification was applied to 1702 patients(retrospective validation cohort) from hospitals outside SYSUCC and 1613 patients(prospective validation cohort) from SYSUCC.The efficacy of radiochemotherapy and radiotherapy modalities was compared between the proposed subtypes.We used Cox proportional hazards models to estimate hazard ratios(HRs) with 95% confidence intervals(CI) for overall survival(OS).Results:The 5?year OS rates for all NPC patients who were diagnosed with epithelial carcinoma(EC;3708 patients),mixed sarcomatoid?epithelial carcinoma(MSEC;1247 patients),sarcomatoid carcinoma(SC;823 patients),and squamous cell carcinoma(SCC;253 patients) were 79.4%,70.5%,59.6%,and 42.6%,respectively(P < 0.001).In mul?tivariate models,patients with MSEC had a shorter OS than patients with EC(HR = 1.44,95% CI = 1.27–1.62),SC(HR = 2.00,95% CI = 1.76–2.28),or SCC(HR = 4.23,95% CI = 3.34–5.38).Radiochemotherapy significantly improved survival compared with radiotherapy alone for patients with EC(HR 49–0.75),and possibly for those with SCC(HR = 0.67,95% CI = 0.56–0.80),MSEC(HR = 0.58,95% CI = 0..74–1.28).= 0.63;95% CI = 0.40–0.98),but not for patients with SC(HR = 0.97,95% CI = 0Conclusions:The proposed classification offers more information for the prediction of NPC prognosis compared with the WHO classification and might be a valuable tool to guide treatment decisions for subtypes that are associ?ated with a poor prognosis. | Hai-Yun Wang Yih-Leong Chang Ka-Fai To Jacqueline S.G.Hwang Hai-Qiang Mai Yan-Fen Feng Ellen T.Chang Chen-Ping Wang Michael Koon Ming Kam Shie-Lee Cheah Ming Lee Li Gao Hui-Zhong Zhang Jie-Hua He Hao Jiang Pei-Qing Ma Xiao-Dong Zhu Liang Zeng Chun-Yan Chen Gang Chen Ma-Yan Huang Sha Fu Qiong Shao An-Jia Han Hai-Gang Li Chun-Kui Shao Pei-Yu Huang Chao-Nan Qian Tai-Xiang Lu Jin-Tian Li Weimin Ye Ingemar Ernberg Ho Keung Ng Joseph T.S.Wee Yi-Xin Zeng Hans-Olov Adami Anthony T.C.Chan Jian-Yong Shao | 2016 | Chinese Journal of Cancer2016，35，6： | 10 |
| 4 | Effects of Primary Annealing Condition on Recrystallization Texture in a Grain Oriented Silicon Steel显示文摘The recrystallization texture in grain oriented silicon steel sheets, which were annealed at different primary annealing temperatures with and without an electric field, was investigated. An automated electron backscattered diffraction (EBSD) technique was used to analyze the recrystallization texture. It was found that recovery and application of electric field in primary annealing lead to an increase of {001} component and a decrease of {111} component after annealing at 900℃. The development of recrystallization texture can be explained in terms of the effects of electric field and primary annealing temperature on recovery. | Yubui SHA Fang ZHANG Song LI Xaoyu GAO Jiazhen XU Liang ZUO School of Materials and Metallurgy,Northeastern University,Shenyang 110004,China | 2004 | Journal of Materials Science & Technology2004，20，3： | 9 |
| 5 | Toric Intraocular Lens vs. Peripheral Corneal Relaxing Incisions to Correct Astigmatism in Eyes Undergoing Cataract Surgery显示文摘Purpose:.To compare toric intraocular lens implantation(ToricIOL).with peripheral corneal relaxing incisions(PCRIs) for astigmatism correction in patients undergoing cataract surgery.Methods: 54 patients(54 eyes) with more than 0.75 diopter(D).of preexisting corneal astigmatism were classified as group A(0.75-1.50D) or group B(1.75-2.50D). The patients were randomized to undergo Toric-IOL or PCRIs in the steep axis with spherical IOL implantation..Log MAR uncorrected visual acuity(Log MAR UCVA), Log MAR best corrected vi sual acuity.(Log MAR BCVA),.error of vector(|EV|), surgery induced refraction correction.(|SIRC |),.and correction rates(CR) were measured 1 month and 6 months postoperatively.Results: At 6 months postoperatively, all 54 eyes had LogMAR BCVA≤0.2. Patients who underwent PCRIs and ToricIOL with Log MAR BCVA≤0.1 showed no significant differences in group A(P=1.00) or in group B(P=0.59). Group A showed no significant differences in Log MAR UCVA(P =0.70), |EV|(P=0.13), |SIRC|(P=0.71), and CR(P=0.56)in patients underwent PCRIs and Toric-IOL. However, group B showed significant differences in Log MAR UCVA(P <0.01), |EV|(P<0.01)), |SIRC|(P<0.01), and CR(P<0.01).The Log MAR UCVA and |EV | between 1 and 6 months showed no significant differences in patients in group A. However, in group B, they are significant differences.Conclusion:.The efficacy and stability of Toric-IOL and PCRIs were equal in low astigmatic patients..Toric-IOL achieved an enhanced effect over PCRIs in higher astigmatic patients.PCRIs had the more refractive regression than Toric-IOL in 6months. | Zhiping Liu Xiangyin Sha Xuanwei Liang Zhonghao Wang Jingbo Liu Danping Huang | 2014 | Eye Science2014，29，4： | 9 |
| 6 | Use of Silicone Tubes to Repair Canalicular Lacerations via a Novel Method显示文摘Purpose:To develop a novel method to repair canalicular lacerations using silicone tubes.Methods:A total of 47 adult patients(47 eyes)with canalicular lacerations were collected from the outpatient department from November 2010 to December 2012.The age ranged from16 to 53 years.Among the 47 eyes,37 had lower canalicular lacerations,6 had upper canalicular lacerations,and 4 had bicanalicular lacerations..A soft probe was made using a stainless steel acupuncture needle,which was inserted into the lumen of the proximal part of the catheter to increase its rigidity.The probe was then inserted into the lacrimal sac and nasolacrimal duct.After retrieval of the catheters,the two ends of the silicone tube were securely tied.(end to end).to the catheters..The silicon tube outside the nostril formed a Ushape.The catheters were then pulled upward until the silicone tube was completely located in the canalicular system.The catheters were cut off of the silicone tube near the site of the connection.The two ends of the silicone tube were cut short,~2mm out of the lacrimal punctum,and tied securely,end to end.The length of the tube between the upper and lower punctum was adjusted to ensure that no tension was present in the medial cathus,and the suture was removed through the nostril.The silicone tube was removed 3-10 months after this novel canalicular intubation procedure (NCI).Results:.All cases were anatomically rehabilitated after surgery..The silicone tube was removed after implanted in 3-10months (mean 4.5±1.3 months),the average follow-up time was 11.8 months after removal.In total,45 eyes in all 47eyes (95.74%)were free from obstruction.Among them,41eyes (91.11%) achieved complete success(completely disap-pearance of epiphora after tube removal),.4 eyes.(8.89%)achieved partial success.(irritation occurs under stimulation conditions,such as wind or cold conditions),4 eyes showed postoperative tearing,with three eyes having inferior lacrimal duct laceration,and one eye with superior canalicular laceration.Apart from two cases (4.26%) suffering inferior punctum splitting,no other associated issues occurred with the silicone tube or iatrogenic injury and lacrimal complications.Conclusion:For adult patients with canalicular laceration,the NCI was an effective,atraumatic surgery,which has fewer complications than traditional canalicular suture. | Zhiping Liu Xiangyin Sha Xuanwei Liang Zhonghao Wang | 2013 | Eye Science2013，28，4： | 8 |
| 7 | The Relationship between Tectonic Subsidence and BSR of Upper Neogene in the Deep-Water Area of the Northern Continental Slope, South China Sea显示文摘BSR (Bottom Simulating Reflector) occurs widely in the strata since the late Miocene in the deep-water area of the northern continental slope of South China Sea (SCS). It is an important seismic reference mark which identifies the gas hydrate and its distribution influenced by the tectonic movements. Single-point basin modeling was conducted using 473 points in the study area. To discuss the relationships between the tectonic subsidence and BSR, the volume and rate of tectonic subsidence in each geological time have been simulated. The results show that there are three tectonic accelerate subsidence processes in the study area since the late Miocene, especially since 1.8Ma the tectonic subsidence accelerates more apparently. Since the Late Miocene to Pleistocene, the rate of tectonic subsidence in deep-water underwent a transformation from weak to strong. The ratio of tectonic subsidence to the total subsidence was relatively high (65-70%). Through the superposition of the BSR developed areas and the contours of tectonic subsidence in this area, it was discovered that more than 80% of BSR tend to be distributed at the slope break or depression-uplift structural transfer zone and the average tectonic subsidence rate ranges from 70 m/Ma to 125 m/Ma. | YU Xinghe WANG Jianzhong LI Shengli FANG Jingnan JIANG Longyan CONG Xiaorong LIANG Jinqiang SHA Zhibin | 2013 | Acta Geologica Sinica(English Edition)2013，87，3： | 6 |
| 8 | Single-seed sowing increased pod yield at a reduced seeding rate by improving root physiological state of Arachis hypogaea显示文摘Double-seed sowing(two seeds per hole)is the dominant pattern of peanut sowing in China,but within-hole plant competition usually limits their growth and yield formation.Besides,the traditional double-seed sowing method does not facilitate mechanization during sowing.The objective of this study was to determine if single-seed sowing at a proper seeding rate yielded better than traditional double-seed sowing pattern and the differences of physiological metabolism of roots.A field experiment was conducted in two consecutive years to compare pod yields of single-seed sowing at 180000(S180),225000(S225),and 270000 seeds ha^-1(S270)with that of double-seed sowing at 270000 seeds ha^-1(D270)using a completely randomized block design with four replications.And the root bleeding sap rate,nutrient content,and the main hormone contents in root bleeding sap were also comparatively investigated.Although the pod yields of single-seed sowing at the three densities were higher than that of traditional double-seed sowing(D270),S225 yielded better than the other two single-seed sowing treatments(S180 and S270).The increased pod yield in single-seed sowing at 225000 seeds ha^-1 was mainly due to the higher pod dry weight per plant and harvest index.The improved pod dry weight and shoot growth had closely relationship with the enhanced root physiological traits such as the increased root bleeding sap rate,content of free amino acids,soluble sugars,K^+,Mg^2+,Zn^2+,and Ca^2+of the individual plant root.The improved activity of root reductive,nitrate reductase(NR)and ATPase and higher zeatin and zeatin riboside(Z+ZR)content of root bleeding sap were alsocrucial to the pod and shoot growth of peanut.Single-seed sowing at a moderate seeding rate(S225)is a potential practice to increase pod yield and to save seed cost. | LIANG Xiao-yan GUO Feng FENG Ye ZHANG Jia-lei YANG Sha MENG Jing-jing LI Xin-guo WAN Shu-bo | 2020 | Journal of Integrative Agriculture2020，19，4： | 6 |
| 9 | Spatial distribution of surface energy fluxes over the Loess Plateau in China and its relationship with climate and the environment显示文摘China's Loess Plateau is located at the edge of the Asian summer monsoon in a transition zone of climate and ecology. In the Loess Plateau, climate and environments change along with space, which has an obvious impact on the spatial distribution of surface energy fluxes. Because of scarce land-surface observation sites and short observation time in this area, previous studies have failed to fully understand the land-surface energy balance characteristics over the entire the Loess Plateau and their effect mechanisms. In this paper, we first test the simulation ability of the Community Land Model(CLM) model by comparing its simulated data with observed data. Based on the simulation data for the Loess Plateau over the past thirty years, we then analyze the spatial distribution of surface energy fluxes and compare the pattern differences between the area averages for the driest year and wettest year. Furthermore, we analyze the relationship between the spatial distribution of the components of the surface energy balance with longitude, latitude, altitude, precipitation and temperature. The main results are as follows: the spatial distribution of surface energy fluxes are significantly different, with the surface net radiation and sensible heat flux increasing from south to north and latent heat flux and soil heat flux decreasing from southeast to northwest. The sensible heat flux at the driest point is nearly twice as high as that at the wettest point, whereas the latent heat flux and soil heat flux at the driest point are half as much as that at the wettest point. The impact of variations of annual precipitation on the components of the surface energy balance is also obvious, and the maximum magnitude of the changes to the sensible heat flux and latent heat flux is nearly 30%. To a certain extent, geographical factors(including longitude, latitude, and altitude) and climate factors(including temperature and precipitation) affect the surface energy fluxes. However, the surface net radiation is more closely related to latitude and altitude, sensible heat flux is more closely related to the monsoon rainfall and latitude, and latent heat flux and soil heat flux are more closely related to the monsoon rainfall. | ZHANG Qiang ZHANG Liang HUANG Jing ZHANG LiYang WANG WenYu SHA Sha | 2014 | Science China Earth Sciences2014，57，9： | 6 |
| 10 | Biofilm-inspired adhesive and antibacterial hydrogel with tough tissue integration performance for sealing hemostasis and wound healing显示文摘Uncontrolled bleeding and infection can cause significant increases in mortalities.Hydrogel sealants have attracted extensive attention for their ability to control bleeding.However,because interfacial water is a formidable barrier to strong surface bonding,a challenge remains in finding a product that offers robust tissue adhesion combined with anti-infection properties.Inspired by the strong adhesive mechanism of biofilm and mussels,we report a novel dual bionic adhesive hydrogel(DBAH)based on chitosan grafted with methacrylate(CS-MA),dopamine(DA),and N-hydroxymethyl acrylamide(NMA)via a facile radical polymerization process.CS-MA and DA were simultaneously included in the adhesive polymer for imitating the two key adhesive components:polysaccharide intercellular adhesin(PIA)of staphylococci biofilm and 3,4-dihydroxy-L-phenylalanine(Dopa)of mussel foot protein,respectively.DBAH presented strong adhesion at 34 kPa even upon three cycles of full immersion in water and was able to withstand up to 168 mm Hg blood pressure,which is significantly higher than the 60–160 mm Hg measured in most clinical settings.Most importantly,these hydrogels presented outstanding hemostatic capability under wet and dynamic in vivo movements while displaying excellent antibacterial properties and biocompatibility.Therefore,DBAH represents a promising class of biomaterials for high-efficiency hemostasis and wound healing. | Wei Han Bo Zhou Kai Yang Xin Xiong Shifang Luan Yu Wang Zheng Xu Peng Lei Zhengshan Luo Jian Gao Yijing Zhan Guopu Chen Lei Liang Rui Wang Sha Li Hong Xu | 2020 | Bioactive Materials2020，5，4： | 6 |
| 11 | A User Requirement Oriented Web Service Discovery Approach Based on Logic and Threshold Petri Net显示文摘In recent years, the number of Web services has increased significantly. Web service discovery has drawn much attention with the development of Web service applications and big data analysis. Under this circumstance, traditional Web service discovery strategies cannot adequately meet high user requirements due to the efficiency and precision of service discovery is low. In order to improve the accuracy and efficiency of service discovery, a user requirement oriented Web service discovery approach based on Petri nets is proposed in this study. A data preprocessing strategy of Web service is first designed. Then, a service clustering method is proposed based on Petri nets, which can conduct service cluster head generation, service cluster composition, and service discovery. The proposed method utilizes a superior data preprocessing method. Using simulation experiments,the efficiency and precision of Web service discovery are illustrated. Finally, the application value of the approach on real Web service is discussed. | Jing Sha Yuyue Du Liang Qi | 2019 | IEEE/CAA Journal of Automatica Sinica2019，6，6： | 5 |
| 12 | Warburg Effects in Cancer and Normal Proliferating Cells:Two Tales of the Same Name显示文摘It has been observed that both cancer tissue cells and normal proliferating cells(NPCs)have the Warburg effect.Our goal here is to demonstrate that they do this for different reasons.To accomplish this,we have analyzed the transcriptomic data of over 7000 cancer and control tissues of 14 cancer types in TCGA and data of five NPC types in GEO.Our analyses reveal that NPCs accumulate large quantities of ATPs produced by the respiration process before starting the Warburg effect,to raise the intracellular pH from 6.8 to 7.2 and to prepare for cell division energetically.Once cell cycle starts,the cells start to rely on glycolysis for ATP generation followed by ATP hydrolysis and lactic acid release,to maintain the elevated intracellular pH as needed by cell division since together the three processes are pH neutral.The cells go back to the normal respirationbased ATP production once the cell division phase ends.In comparison,cancer cells have reached their intracellular pH at 7.4 from top down as multiple acid-loading transporters are up-regulated and most acid-extruding ones except for lactic acid exporters are repressed.Cancer cells use continuous glycolysis for ATP production as way to acidify the intracellular space since the lactic acid secretion is decoupled from glycolysis-based ATP generation and is pH balanced by increased expressions of acid-loading transporters.Co-expression analyses suggest that lactic acid secretion is regulated by external,non-pH related signals.Overall,our data strongly suggest that the two cell types have the Warburg effect for very different reasons. | Huiyan Sun Liang Chen Sha Cao Yanchun Liang Ying Xu | 2019 | Genomics, Proteomics & Bioinformatics2019，17，3： | 4 |
| 13 | A Teosinte-derived Allele of a MYB Transcription Repressor Confers Multiple Disease Resistance in Maize.显示文摘Natural alleles controlling multiple disease resistances (MDR) are valuable for crop breeding. However, only one MDR gene have been cloned in maize, and molecular mechanisms of MDR are not clear. By map-based cloning, we have cloned a teosinte-derived allele of a resistance gene, Mexicana lesion mimic 1 (ZmMM1), which has a lesion mimic phenotype and confers resistance to northern leaf blight (NLB), gray leaf spot (GLS) and southern corn rust (SCR). Strong MDR conferred by the teosinte allele is linked with the polymorphisms in the 3' untranslated region of the ZmMM1 gene that cause increased accumulation of ZmMM1 protein. ZmMM1 acts as a transcription repressor and negatively regulates transcription of specific target genes including ZmMM1-target gene 3 (ZmMT3), which functions as a negative regulator of plant immunity and associated cell death. The successful isolation of the ZmMM1 resistance gene will help not only in developing broad-spectrum and durable disease resistance but also in understanding the molecular mechanisms underlying MDR. | Hongze Wang Jiabao Hou Pei Ye Long Hu Junshi Huang Zhikang Dai Bao Zhang Sha Dai Jiamin Que Haoxuan Min Gengshen Chen Yanbo Wang Min Jiang Yan Liang Lin Li Xuecai Zhang Zhibing Lai | 2021 | Molecular Plant2021，14，11： | 4 |
| 14 | Cochlear implantation effect on deaf children with gap junction protein beta 2 gene mutation显示文摘 | KONG Ying LIU Sha WANG Su-ju Li Shu-jing LIANG Shuang | 2013 | Chinese Medical Journal2013，，7： | 4 |
| 15 | Regioselective synthesis and initial evaluation of a folate receptor targeted rhaponticin prodrug显示文摘改进 rhaponticin (RHA ) 的治疗学的效果,叶酸的受体(FR ) 指向了 RHA prodrug 被利用经由一个能释放的二硫化物连接器连接到 folic 酸(FA ) 的一个吸水的肽分隔符设计并且 regioselectively 综合了。一系列生物评估在 vitro 并且在 vivo 被调查。生物调查的积极结果在指向的这篇小说前保证进一步现出症状之前的潜的学习化学疗法为临床的调查被考虑。 | Xu Hua Liang Yang Sun Lu Sha Liu Ying Yong Zhao Xiao Yun Hu Jun Fan | 2012 | Chinese Chemical Letters2012，23，10： | 3 |
| 16 | Evolution of the Late Miocene atoll systems offshore Xisha Islands显示文摘Modern atolls have been studied systematically and thoroughly in the South China Sea.However,the knowledge of a paleo-atoll and related sedimentary system is very limited.Here we used the newly acquired high resolution 2D seismic data,and discovered three late Miocene atoll systems in the offshore Xisha Islands for the first time.We named them atoll system A,B,C,respectively.These three atoll systems,all developed on the horsts dominated by normal fault,consist mainly of atoll reefs,patch reefs,fore-reef slope deposits,and lagoons.On the basis of the interpreted sequence stratigraphic framework and the identification of fore-reef slope deposits,we suggested only the atoll system A continued to grow until Quaternary,and both of atoll system B and C had been drowned in Pliocene.In late Miocene,the atoll systems in the study area were most developed,either in magnitude or in maturity,which indicated late Miocene was the most flourishing period of reef builders,and this was in accordance with the drilling result of ODP Leg 184 in the South China Sea.Pliocene was an important reef drowning period in the study area,and both atoll systems B and C were drowned and hemipelagic deposits prevailed gradually.Quaternary was another reef drowning period in the study area,two large atoll reefs grown on the atoll system A were finally drowned,and hemipelagic deposits begun to drape and fill the palaeo-atoll systems.The growth and drowning of atolls are controlled mainly by tectonic subsidence in long term,but global eustatic can impact it in short term also. | KUANG ZengGui GUO YiQun WANG LiaoLiang LIANG JinQiang SHA ZhiBin | 2014 | Science China Earth Sciences2014，57，12： | 3 |
| 17 | Texture Evolutions in Fe-6.5%Si Produced by Rapid Solidification and Chemical Vapor Deposition显示文摘Fe-Si ribbons and thin sheets with 6.5%Si content were prepared by means of the single roller rapid solidification and chemical vapor deposition (CVD), respectively. The initial textures of rapidly solidified Fe-6.5%Si ribbons were characteristic of the {100} fiber-type, which became weakened during primary recrystallization in various atmospheres.At the stage of secondary recrystallization, the {100} texture formed in Ar and the {110} texture in hydrogen, while there occurred a texture transformation from the {100} type to the {110} type in vacuum with the increase of annealing temperature. For Fe-6.5%Si sheets prepared by Si deposition in cold-rolled Fe-3%Si matrix sheets, their textures were dominated by the η-fiber (<001>//RD) with the maximum density at the {120}<001> orientations.After homogenization annealing, the η-fiber could evolve into the {130}<001> type or become more concentrated on the {120}<001> orientations, depending on the cold rolling modes of Fe-3%Si matrix sheets. | Liang ZUO Guangyong HU Yuhui SHA Claude ESLING | 2002 | Journal of Materials Science & Technology2002，18，6： | 3 |
| 18 | Functional characterization and key residues engineering of a regiopromiscuity O-methyltransferase involved in benzylisoquinoline alkaloid biosynthesis in Nelumbo nucifera显示文摘Lotus(Nelumbo nucifera),an ancient aquatic plant,possesses a unique pharmacological activity that is primarily contributed by benzylisoquinoline alkaloids(BIAs).However,only few genes and enzymes involved in BIA biosynthesis in N.nucifera have been isolated and characterized.In the present study we identified the regiopromiscuity of an O-methyltransferase,designated NnOMT6,isolated from N.nucifera;NnOMT6 was found to catalyze the methylation of monobenzylisoquinoline 6-O/7-O,aporphine skeleton 6-O,phenylpropanoid 3-O,and protoberberine 2-O.We further probed the key residues affecting NnOMT6 activity via molecular docking and molecular dynamics simulation.Verification using site-directed mutagenesis revealed that residues D316,N130,L135,N176A,D269,and E328 were critical for BIA O-methyltransferase activities;furthermore,N323A,a mutant of NnOMT6,demonstrated a substantial increase in catalytic efficiency for BIAs and a broader acceptor scope compared with wild-type NnOMT6.To the best of our knowledge,this is the first study to report the O-methyltransferase activity of an aporphine skeleton without benzyl moiety substitutions in N.nucifera.The study findings provide biocatalysts for the semisynthesis of related medical compounds and give insights into protein engineering to strengthen O-methyltransferase activity in plants. | Yuetong Yu Yan Liu Gangqiang Dong JinZhu Jiang Liang Leng XianJu Liu Jun Zhang An Liu Sha Chen | 2023 | Horticulture Research2023，10，2： | 3 |
| 19 | Site-directed mutagenesis identified the key active site residues of alcohol acyltransferase PpAAT1 responsible for aroma biosynthesis in peach fruits显示文摘The aroma of peach fruit is predominantly determined by the accumulation of γ-decalactone and ester compounds.A previous study showed that the biosynthesis of these aroma compounds in peach fruit is catalyzed by PpAAT1,an alcohol acyltransferase.In this work,we investigated the key active site residues responsible for γ-decalactone and ester biosynthesis.A total of 14 candidate amino acid residues possibly involved in internal esterification and 9 candidate amino acid residues possibly involved in esterification of PpAAT1 were assessed via site-directed mutagenesis.Analyses of the in vitro enzyme activities of PpAAT1 and its site-directed mutant proteins(PpAAT1-SMs)with different amino acid residue mutations as well as the contents of γ-decalactone in transgenic tobacco leaves and peach fruits transiently expressing PpAAT1 and PpAAT1-SMs revealed that site-directed mutation of H165 in the conserved HxxxD motif led to lost enzymatic activity of PpAAT1 in both internal esterification and its reactions,whereas mutation of the key amino acid residue D376 led to the total loss ofγ-decalactone biosynthesis activity of PpAAT1.Mutations of 9 and 7 other amino acid residues also dramatically affected the enzymatic activity of PpAAT1 in the internal esterification and esterification reactions,respectively.Our findings provide a biochemical foundation for the mechanical biosynthesis ofγ-decalactone and ester compounds catalyzed by PpAAT1 in peach fruits,which could be used to guide the molecular breeding of new peach species with more favorable aromas for consumers. | Zhi-Zhong Song Bin Peng Zi-Xia Gu Mei-Ling Tang Bei Li Mei-Xia Liang Li-Min Wang Xiao-Tong Guo Jian-Ping Wang Yu-Fen Sha Hong-Xia Zhang | 2021 | Horticulture Research2021，8，1： | 3 |
| 20 | Synthesis of hyperbranched copolymers by combining enzymatic ring-opening polymerization and ATRP from a novel bifunctional initiator显示文摘亢奋的分叉的脂肪族的聚酯 P (epsilon -CL) 借助于 BHB 的催化 Novozyme435 的戒指洞聚合(ROP ) 被综合(2, 2 二度(hydroxymethyl ) 奶油的酸) 。以 P ( epsilon-CL )的氢氧根结束的链被 esteriflcation ofa-bromopropionyl 溴化物修改获得亢奋的分叉的 difunctional 宏开始者,它在圣库克尔姆泰塔的 theATRP 被使用在 ATRP 的反应被用作催化剂系统 polystyrene-block-poly 获得 thehyperbranched 共聚物(2,2二度( hydroxymethyl )奶油的酸)。共聚物被 NMR 和 GPC 证实。 | AI Peng CHEN Liang HU Dehua CHEN Yanhua LU Dongshuang ZHANG Bao SHA Ke WANG Jingyuan | 2006 | Chinese Science Bulletin2006，51，22： | 3 |
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