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| 1 | Anticancer effects of oligomeric proanthocyanidins on human colorectal cancer cell line, SNU-C4显示文摘AIM: Oligomeric proanthocyanidins (OPC), natural polyphenolic compounds found in plants, are known to have antioxidant and anti-cancer effects. We investigated whether the anti-cancer effects of the OPC are induced by apoptosis on human colorectal cancer cell line, SNU-C4.METHODS: Colorectal cancer cell line, SNU-C4 was cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum. The cytotoxic effect of OPC was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylt-etrazolium bromide (MTT) assay. To find out the apoptotic cell death, 4, 6-diamidino-2-phenylindole (DAPI) staining,terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcriptionpolymerase chain reaction (RT-PCR), and caspase-3 enzyme assay were performed.RESULTS: In this study, cytotoxic effect of OPC on SNUC4 cells appeared in a dose-dependent manner. OPC treatment (100 μg/mL) revealed typical morphological apoptotic features. Additionally OPC treatment (100 μg/mL)increased level of BAX and CASPASE-3, and decreased level of BCL-2 mRNA expression. Caspase-3 enzyme activity was also significantly increased by treatment of OPC (100 μg/mL) compared with control.CONCLUSION: These data indicate that OPC caused cell death by apoptosis through caspase pathways on human colorectal cancer cell line, SNU-C4. | Youn-Jung Kim Hae-Jeong Park Seo-Hyun Yoon Mi-Ja Kim Kang-Hyun Leem Joo-Ho Chung Hye-Kyung Kim | 2005 | World Journal of Gastroenterology2005，11，30： | 8 |
| 2 | Extrusion process of Acanthopanax senticosus leaves enhances the gastroprotective effect of compound 48/80 on acute gastric mucosal lesion in rats显示文摘OBJECTIVE:To investigate the gastroprotective effects of Acanthopanax senticosus leaves(ASLs)extrusion on acute gastric mucosal lesion in rats induced by compound 48/80(C48/80).METHODS:Rats were divided into six groups:normal;C48/80-induced gastric lesion control;gastric lesion positive control(famotidine 4 mg/kg);gastric lesion administered with two levels of extruded ASLs(ASLE,40 and 200 mg/kg);and gastric lesion treated with ASLs(ASL 200 mg/kg).Mucus secretion/damage was determined by immunohistological staining.Immunofluorescence and western blotting were performed to determine gastric mucosal Bax and Bcl-2 expression.Gastric mucosal oxidative-stress-related enzymes and malondialdehyde were determined.RESULTS:C48/80-induced mucus depletion and inflammation in the gastric mucosa were significantly attenuated by ASLs.The increased serum serotonin and histamine concentrations in C48/80-treated rats were also attenuated by ASLs.Gastric mucosal Bax protein expression was increased and Bcl-2 expression was decreased after C48/80 treatment,and ASLs ameliorated Bax and Bcl-2 expression.The extrusion process significantly augmented the effects of ASLs in a dosedependent manner.ASLEs at 200 mg/kg normalized mucus damage/secretion,C48/80-induced increases of mucosal myeloperoxidase activity(index of inflammation),xanthine oxidase,and malondialdehyde content(index of lipid peroxidation).The effects of ASLs on Bax and Bcl-2expression were also enhanced by extrusion.Furthermore,these effects of ASLEs at 200 mg/kg were similar to those of famotidine,a histamine H2-receptor antagonist commonly used to treat gastric ulcers.CONCLUSION:ASLEs prevented acute gastric mucosal lesion progression induced by C48/80,possibly by inducing mucus production,and reduced inflammation and oxidative stress in gastric mucosa through an anti-apoptotic mechanism. | Hye Kyung Kim Myung-Gyou Kim Kang-Hyun Leem | 2016 | Journal of Traditional Chinese Medicine2016，36，2： | 3 |
| 3 | The distribution and ecology of Malassezia furfur and cutaneous bacteria on human skin显示文摘 | Leeming JP Notman PH Holland KT | 1989 | J Appl Bacteriol1989，67，： | 2 |
| 4 | 运动计时系统中的多阅读器算法及其在低频带宽中的应用(英文)显示文摘In sports timing systems,P2P communication is used at low frequency bandwidths(under 135 KHz) between tags and readers in the RFID field.However,in such cases,collisions tend to occur when a reader deals with multiple RFID tags simultaneously.To overcome this issue,a sports timing system including a Multi Reader Controller(MRC)loaded with an advanced multiple reader algorithm and application was created and applied at large-scale citizens' marathon events.In these cases,a large number of people pass over the installed urethane type's antenna mat continually during a short period of time.This study verified the superiority of the improved algorithm and application through the on-thespot application of the multi reader algorithm and application program,which allows us to smoothly measure runners' times through multiaccess reading for rapid collision avoidance. | Min-Ho Paek Choon-Seong Leem Dae-Jung Bae | 2013 | China Communications2013，10，12： | 2 |
| 5 | Uniaxially crumpled graphene as a platform for guided myotube formation显示文摘Graphene,owing to its inherent chemical inertness,biocompatibility,and mechanical flexibility,has great potential in guiding cell behaviors such as adhesion and differentiation.However,due to the two-dimensional(2D)nature of graphene,the microfabrication of graphene into micro/nanoscale patterns has been widely adopted for guiding cellular assembly.In this study,we report crumpled graphene,i.e.,monolithically defined graphene with a nanoscale wavy surface texture,as a tissue engineering platform that can efficiently promote aligned C2C12 mouse myoblast cell differentiation.We imparted out-of-plane,nanoscale crumpled morphologies to flat graphene via compressive straininduced deformation.When C2C12 mouse myoblast cells were seeded on the uniaxially crumpled graphene,not only were the alignment and elongation promoted at a single-cell level but also the differentiation and maturation of myotubes were enhanced compared to that on flat graphene.These results demonstrate the utility of the crumpled graphene platform for tissue engineering and regenerative medicine for skeletal muscle tissues. | Junghoon Kim Juyoung Leem Hong Nam Kim Pilgyu Kang Jonghyun Choi Md Farhadul Haque Daeshik Kang SungWoo Nam | 2019 | Microsystems & Nanoengineering2019，5，1： | 2 |
| 6 | Transaiptiona activation of the Cdk inhibitor p21 by vitaminD3 lead to the induced differentiation of the myelomonocytic cells line U937显示文摘 | Liu M LeeM Cohen M etal | 1996 | Genes Dev1996，10，： | 1 |
| 7 | Purification and Characterization of N-beta-Alanyl-5-S-glutathionyl-3,4-dihydrox-yphenylalanine,a Novel Antibacterial Substance of Sarcop haga peregrina (Flesh Fly)显示文摘 | Leem J Y Nishimura C Kurata S | 1996 | J Biol Chem1996，271，13： | 1 |
| 8 | Critical Review of Behavioral Interventions to Preserve the Environment,Research Since 1980显示文摘 | Dwyer W O Leeming F C Cobern M K | | 0，，05： | 1 |
| 9 | A reference system for Internet based inter-enterprise electronic commerce显示文摘 | SHIN K LEEM C S | 2002 | Journal of Systems and Software2002，60，3： | 1 |
| 10 | Two cases of gamma knife radiosurgery for low-grade optic chiasm glioma显示文摘 | Lim YJ Leem W | 1996 | Stereotact Funct Neurosurg1996，66，11： | 1 |
| 11 | Characterization of Lactobacillus plantarum PH 04, a potential probiotic bacterium with cholesterol-lowering effects显示文摘 | Nguyen T D T Kang JH Leem S | 2007 | Int J Food Microbiol2007，113，1： | 1 |
| 12 | AccurateandexactCNVidentificationfromtargetedhighthroughputsequencedata显示文摘 | NORDAS LEEM KINGM C etal | 2011 | BMCGenomics2011，12，： | 1 |
| 13 | Headway on urban streets: observational data and an intervention to decrease tailgating显示文摘 | MICHAEL P G LEEMING F C DWYER W O | 2000 | Transporta- tion Research Part F2000，3，2： | 1 |
| 14 | Supercharging and augmenting venous drainage of an anterolateral thigh free flap: options and indications显示文摘 | Deleyiannis FW Badeau AM Leem TH | 2014 | Plast Reconstr Surg Glob Open2014，2，4： | 1 |
| 15 | Light-induced covalent immobilization of monolayers of magnetic nanoparticles on hydrogen-terminated silicon显示文摘 | Leem G Zhang S Jamison A C | 2010 | ACS Applied Materials&Interfaces2010，2，10： | 1 |
| 16 | Limited therapeutic potential of astrocyte elevated gene-1 transduction in an animal model of Parkinson’s disease显示文摘Biological roles of astrocyte elevated gene-1(AEG-1):AEG-1,also known as metadherin,was originally identified as a human immunodeficiency virus-1-and tumor necrosis factor-alpha-inducible gene in human fetal astrocytes.The increase in AEG-1 expression is a well-established and important oncogenic event in various types of human cancer,and its upregulation triggers evasion of cellular apoptosis,metastasis,and invasion in cancer(Emdad at al.,2016;Dhiman et al.,2019).AEG-1 can promote tumor progression via multiple phosphoinositide 3-kinase(PI3K)/protein kinase B(Akt)pathways,contributing to an invasive phenotype and angiogenesis(Emdad at al.,2016;Dhiman et al.,2019).In addition,there is evidence for a functional link between AEG-1 and pro-survival mechanisms in various cancers(Dhiman et al.,2019),suggesting that AEG-1 is a key molecule for oncogenic events in cancer. | Eunju Leem Sang Ryong Kim | 2020 | Neural Regeneration Research2020，15，10： | 1 |
| 17 | Amount of retainedaustenite at room temperature after reversetransformation of martensiteto austenite in an Fe - 13 percent Cr - 7 percent Ni - 3 percent Simartensiticstainless steel 显示文摘 | LEEM Dong-seok LEE Yong-deuk JUN Joong-hwan | 2001 | Structural Materials2001，，10： | 1 |
| 18 | Cloning of an acidic laccase gene (clac2) from Coprinus congregatus and its expression by external pH显示文摘 | Kim S Leem Y Kim K | 2001 | FEMS Microbiol Lett2001，195，2： | 1 |
| 19 | Diagnosis of inva- sive aspergillosis in bone marrow transplant recipients by poly- merase chain reaction显示文摘 | Williamson E C Leeming J P Palmer H M | 2000 | Br J Haematol2000，108，1： | 1 |
| 20 | Is bone quality as- sociated with collagen age Osteoporos 显示文摘 | Leeming DJ Henriksen K Byrjalsen I | 2009 | Int2009，20，： | 1 |
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