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| 1 | Phosphomonoester Phosphoethanolamine Induces Apoptosis in Human Chronic Myeloid Leukemia Cells显示文摘Background:Leukemia is a type of cancer that starts in the blood or blood-forming tissues.It results from the clonal proliferation of hematopoietic cells in the bone marrow and/or lymphoid tissues,which subsequently reach the peripheral circulation and can infiltrate other systems.There are many different kinds of leukemia,and treatments are different for each one.Chronic leukemia is with a slower growing than acute leukemia but could be just as life-threatening.Phospholipids are antitumor analogs,such as synthetic phosphoethanolamine,which is a phosphorylated compound capable of controlling cellular proliferation and inducing apoptosis in several types of tumor cells.Methods:K562 and K562-Lucena(MDR+)human chronic myeloid leukemia cells were treated with synthetic phosphoethanolamine(Pho-s).The viability was evaluated by sulforhodamine B(SRB)assay and cell cycle phases,apoptosis,markers expression,and mitochondrial potential were assessed by flow cytometry.Results:Tumor cells formed clusters in suspension and decreased significantly viability.The concentrations for IC50%were obtained.Pho-s treated were 43.1 mM(K562)and 145.9 mM(K562-Lucena MDR+)in a period of 24 hours.Pho-s induced changes in the distribution of cell population phases of cell cycle which showed an increase in fragmented DNA and increased markers expression envolved apoptosis pathways a decrease in the G1/G0 phase.Discussion:Treatment of K562 and K562-Lucena(MDR+)chronic myeloid leukemia cells with Pho-s showed dose and time dependent cytotoxic effects.This cytotoxicity induced a decrease in proliferative capacity,mitochondrial electrical potential,and consequently release of cytochrome C;inhibition of Bcl-2 family protein expression,increase in pro-apoptotic family members Bad and Bax,dependent on p53 expression.Conclusion:This study presented a significant therapeutic potential of Phos-s in this type of leukemia through the apoptotic effects on tumor cells independently of the molecular resistance profile(MDR+). | Thais de Oliveira Conceicao Manuela Garcia Laveli da Silva Durvanei Augusto Maria | 2019 | Journal of Pharmacy and Pharmacology2019，7，7： | 3 |
| 2 | Meclizine Chloridrate and Methyl-β-Cyclodextrin Associated with Monophosphoester Synthetic Phosphoethanolamine Modulating Proliferative Potential in Triple-Negative Breast Cancer Cells显示文摘Synthetic phosphoethanolamine(Pho-s)is a monophosphoester ester with anti-inflammatory and pro-apoptotic properties.Meclizine chloridrate(MC)is a histamine H1 receptor blocker that is also able to inhibit cellular respiration.However,MC does not inhibit cellular respiration in isolated mitochondria such as antimycin and rotenone.Methyl-β-cyclodextrin(MβCD)belongs to theβ-cyclodextrin family,which is capable of removing cholesterol from the plasma membrane.The aim of this study was to evaluate the proliferative effects of meclizine chloridrate and methyl-β-cyclodextrin compounds associated with synthetic phosphoethanolamine in a triple-negative human breast tumor line,MDA-MB-231 Cell viability of the tumor line and normal cells FN1 was evaluated by MTT colorimetric test;the production of free radicals was determined by lipoperoxidation(LPO)test;and the percentage of cell cycle phases and proliferative index was evaluated by flow cytometry.Cell viability demonstrated a significant decrease with the treatments of MβCD,MC and Pho-s associated with MC.The production of free radicals decreases significantly in all treatments.In addition,a significant increase of DNA fragment and decrease in G0/G1 cell cycle phase were observed in cellular percentage with concentrations of 20 and 30 mM of Pho-s in association with MC and MβCD,respectively. | Manuela Garcia Laveli da Silva Luciana Bastianelli Knop Durvanei Augusto Maria | 2019 | Journal of Pharmacy and Pharmacology2019，7，7： | 2 |
| 3 | Prediction of intestinal absorption: comparative assessment of GASTROPLUS and IDEA显示文摘 | Parrott N Lave T | 2002 | Eur J Pharm Sci2002，17，12： | 1 |
| 4 | Spatial and temporal variability of water percolation and movement in a system of longittidinal dunes, Western Negev, Israel显示文摘 | Yair A Lavee H Greitser N | 1997 | Hydrological processes1997，11，： | 1 |
| 5 | Differences in peroxidase activity ane isoenzymes in embryogenic ane non-embryogenic ' Shamouti' orange ovular callus lines显示文摘 | KOCHBA J LAVEE S SPIEGEL R P | 1977 | Plant Cell Physiol1977，18，2： | 1 |
| 6 | Comparison of a disease-specific and a generic severity of illness measure for patients with community-acquired pneumonia显示文摘 | FINE M J HANUSA BH LAVE JR | 1995 | Chinese Journal of General Internal Medicine1995，10，： | 1 |
| 7 | An analysis of towboat delay显示文摘 | DESALVO J S LAVE L B | 1968 | Journal of Transportation Economy Policy1968，2，： | 1 |
| 8 | Monitoring temporal vegetation coyer changes in mediterranean and arid ecosystems using a remote sensing technique: case study of the Judean mountain and the Judean desert显示文摘 | Shoshany M Lavee K P | 1996 | Journal of Arid Environments1996，33，1： | 1 |
| 9 | Monitoring temporal vegetation cover changes in Mediterranean and arid ecosystems using a remote sensing technique:Case study of the Judean Mountain and the Judean Desert显示文摘 | SHOSHANY M KUTIEL P LAVEE H | 1996 | Journal of Arid Environments1996，33，1： | 1 |
| 10 | Runoff generative process and runoff yield from arid talus mantles slopes 显示文摘 | Yair A Lavee H | 1976 | Earth Surface Processes1976，1，3： | 1 |
| 11 | The long-term life cycle private and external costs of high coal usage in the US显示文摘 | BERGERSON J LAVE L | 2007 | Energy Policy2007，35，12： | 1 |
| 12 | Rock fragments in top soils:Significance and processes显示文摘 | Poesen J Lavee H | 1994 | Catena1994，23，： | 1 |
| 13 | Using input-output analysis to estimate economy widedischarges 显示文摘 | LAVE L B COBAS-FLORES E HENDRICKSON C T etal | 1995 | Environmental Science & Technology1995，29，9： | 1 |
| 14 | Prospective feasibility trial of radiotherapy target definition for head and neck cancer using 3-dimensional PET and CT imaging显示文摘 | Searfone C Lavely WC Cmelak A J | 2004 | J Nucl Med2004，45，4： | 1 |
| 15 | Rock fragments in top soils: significances and processes显示文摘 | Poesen J Lavee H | 1994 | Catena1994，23，： | 1 |
| 16 | Difference in peroxidase activity and isoenzymes in embryogenic and non- embryogenic 'Shamouti' orange ovular callus lines显示文摘 | Kochba J Lavee S Spiege R P | 1997 | Plant Cell Physiol1997，18，： | 1 |
| 17 | Oxidative processes in bud dormancy and the use of hydrogen cyanamide in breaking dormancy显示文摘 | Shulman Y Nir G Lavee S | 1986 | Acta Horticulturae1986，179，： | 1 |
| 18 | Economic input-output models for environment life-cycle assessment显示文摘 | Lave L Hendrickson C Horvath A | 2002 | Environmental Science & Technology2002，32，7： | 1 |
| 19 | A life-cycle comparison of alternative automobile fuels显示文摘 | MACLEAN H L LAVE L B LANKEY R | 2000 | Journal of the Air and Waste Management Association2000，50，10： | 1 |
| 20 | Spatial distribution dynamics of topsoil moisture in shrub microenvironment after rain events in arid and semi-arid areas by means of high resolution maps显示文摘 | Itzhak K Blumberg D G Lavee H | 2007 | Geomorphology2007，86，： | 1 |
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