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| 1 | Rho/Rock signal transduction pathway is required for MSC tenogenic differentiation显示文摘Mesenchymal stem cell(MSC)-based treatments have shown promise for improving tendon healing and repair.MSCs have the potential to differentiate into multiple lineages in response to select chemical and physical stimuli, including into tenocytes. Cell elongation and cytoskeletal tension have been shown to be instrumental to the process of MSC differentiation. Previous studies have shown that inhibition of stress fiber formation leads MSCs to default toward an adipogenic lineage, which suggests that stress fibers are required for MSCs to sense the environmental factors that can induce differentiation into tenocytes. As the Rho/ROCK signal transduction pathway plays a critical role in both stress fiber formation and in cell sensation, we examined whether the activation of this pathway was required when inducing MSC tendon differentiation using rope-like silk scaffolds. To accomplish this, we employed a loss-of-function approach by knocking out ROCK,actin and myosin(two other components of the pathway) using the specific inhibitors Y-27632, Latrunculin A and blebbistatin, respectively. We demonstrated that independently disrupting the cytoskeleton and the Rho/ROCK pathway abolished the expression of tendon differentiation markers and led to a loss of spindle morphology. Together, these studies suggest that the tension that is generated by MSC elongation is essential for MSC teno-differentiation and that the Rho/ROCK pathway is a critical mediator of tendon differentiation on rope-like silk scaffolds. | Edward Maharam Miguel Yaport Nathaniel L Villanueva Takintope Akinyibi Damien Laudier Zhiyong He Daniel J Leong Hui B Sun | 2015 | Bone Research2015，3，3： | 4 |
| 2 | Molecular cloning doublestranded RNA inducible Mx genes from Atlantic salmon (salmo salar L)显示文摘 | Robertsen B Trobridge G D Leong J | 1997 | Dev Comp Immunol1997，21，： | 1 |
| 3 | A security model for distributed product data management system显示文摘 | K K Leong K M Yu W B Lee | 2003 | Computers in Industry2003，50，： | 1 |
| 4 | Response of a residual soil slope to rainfall显示文摘 | Rahardjo H Lee T T Leong E C Rezaur R B | 2005 | Canadian Geotechnical Journal2005，42，： | 1 |
| 5 | 查看详情显示文摘 | Leong W L Mathews N Tan B Vaidyanathan S Dotz F Mhaisalkar S | | 0，，： | 1 |
| 6 | Gaussian random number generators显示文摘 | Thomas D B Luk W Leong P H W etal | 2007 | ACM Computing Surveys2007，39，4： | 1 |
| 7 | Signaling pathways governing osteo-blast proliferation differentiation and function显示文摘 | Chau JF Leong WF Li B | 2009 | Histol His-topathol2009，24，12： | 1 |
| 8 | Cerebrovascular events associated with infusion through arterially malpositioned triple-lumen catheter:report of three cases and review of literature显示文摘 | Shah PM Leong B Babu SC | | 0，，6： | 1 |
| 9 | Measuring the Transaction Sector in the Australian Economy : 1911--1991 显示文摘 | Dollery B Leong W H | 1998 | Australian Economic History Review1998，38，3： | 1 |
| 10 | Comparison of the Mechanical Properties and Interracial Interactions Between Talc, Kaolin, and Calcium Carbonate Filled Polypropylene Composites显示文摘 | Leong Y W Bakar M B Mohd Z A | 2004 | Journal of Applied Polymer Science2004，91，5： | 1 |
| 11 | Teaching Introductory Program- ming as an Online Game显示文摘 | Leong B Koh Z H Razeen A | 2011 | 2011，，： | 1 |
| 12 | Monitoring of an Aeroponic Greenhouse with a Sensor Network显示文摘 | Leong B T Chan T K Sel appan P | | 0，，3： | 1 |
| 13 | Effects of Filler Treatments on the Mechanical, Flow, Thermal, and Morphological Properties of Talc and Calcium Carbonate Filled Polypropylene Hybrid Composites 显示文摘 | Leong Y W Bakar M B Mohd Z A | 2005 | Journal of Applied Polymer Science2005，98，1： | 1 |
| 14 | Pot2, an inverted repeat transposon from the rice blast fungus Magnaporthe grisea显示文摘 | Kachroo P Leong S A Chattoo B B | 1994 | Mol Gen Genet1994，245，： | 1 |
| 15 | Selective induction of immune response by cytokines coexpressed in recombinant fowlpox virus显示文摘 | Leong K H Ramsay A J Boyle D B | 1994 | J Virol1994，68，12： | 1 |
| 16 | Byzantine modification detec- tion in multicast networks with random network coding 显示文摘 | Ho T Leong B Koetter R | 2008 | IEEE Transactions on Information Theory2008，54，6： | 1 |
| 17 | Laparoscopic repair for\sperforated peptic ulcer:a randomized controlled trial显示文摘 | Siu W T Leong H T Law B K B | 2002 | Ann Surg2002，235，3： | 1 |
| 18 | Effect of antecedent rainfall on pore-water pressure distribution characteristics in residual soil slopes under tropical rainfall显示文摘 | RAHARDJO H LEONG E C REZAUR R B | 2008 | Hydrological Processes2008，22，4： | 1 |
| 19 | The role of missha- pen NCK-related kinase (MINK), a novel Ste20 family kinase, in the IRES-mediated protein translation of human Enterovirus 71 显示文摘 | LEONG S Y ONG B K T CHU J J H | 2015 | PIoS Pathog2015，11，10： | 1 |
| 20 | Pot 2,an inverted repeat transposon from the rice fungus Magnaporthe grisea 显示文摘 | Kachroo P Leong S A Chattoo B B | 1994 | Molecular and General Genetics1994，，245： | 1 |
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