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| 1 | Increased catabolism and decreased unsaturation of ganglioside in patients with inflammatory bowel disease显示文摘AIM: To investigate whether accelerated catabolism of ganglioside and decreased ganglioside content contribute to the etiology of pro-inflammatory intestinal disease. METHODS: Intestinal mucosa from terminal ileum or colon was obtained from patients with ulcerative colitis or inflammatory Crohn's disease(n = 11) undergoing bowel resection and compared to control samples of normal intestine from patients with benign colon polyps(n = 6) and colorectal cancer(n = 12) in this observational case-control study. Gangliosides and phospholipids of intestinal mucosa were characterized by class and ceramide or fatty acid composition using liquid chromatography triple-quad mass spectrometry. Content and composition of ganglioside classes GM1, GM3, GD3, GD1 a, GT1 and GT3 were compared among subject groups. Content and composition of phospholipid classes phosphatidylcholine(PC) and phosphatidylethanolamine were compared among subject groups. Unsaturation index of individual ganglioside and phospholipid classes was computed and compared among subject groups. Ganglioside catabolism enzymes beta-hexosaminidase A(HEXA) and sialidase-3(NEU3) were measured in intestinal mucosa using western blot and compared among subject groups. RESULTS: Relative GM3 ganglioside content was 2-fold higher(P < 0.05) in intestine from patients with inflammatory bowel disease(IBD) compared to control intestine. The quantity of GM3 and ratio of GM3/GD3 was also higher in IBD intestine than control tissue(P < 0.05). Control intestine exhibited 3-fold higher(P < 0.01) relative GD1 a ganglioside content than IBD intestine. GD3 and GD1 a species of ganglioside containing three unsaturated bonds were present in control intestine, but were not detected in IBD intestine. The relative content of PC containing more than two unsaturated bonds was 30% lower in IBD intestine than control intestine(P < 0.05). The relative content of HEXA in IBD intestine was increased 1.7-fold(P < 0.05) and NEU3 was increased 8.3-fold(P < 0.01) compared to normal intestine. Intestinal mucosa in IBD is characterized by increased GM3 content, decreased GD1 a, and a reduction in polyunsaturated fatty acid constituents in GD3, GD1 a and PC.CONCLUSION: This study suggests a new paradigm by proposing that IBD occurs as a consequence of increased metabolism of specific gangliosides. | John J Miklavcic Glen K Shoemaker Vera C Mazurak M Tom Clandinin Tasha DL Hart Kareena L Schnabl Gordon M Lees Bodil MK Larsen Oliver F Bathe Alan BR Thomson M Tom Clandinin | 2015 | World Journal of Gastroenterology2015，21，35： | 3 |
| 2 | Sealing PCI tracts with ge1atin matrix hemostatic sealant(FloSea1):initial clinica1 use显示文摘 | Lee DL Uribe C Eichel L | 2004 | J Urol2004，171，21： | 1 |
| 3 | Characterization of an improved double hemorrhage rat model for the study of delayed cerebral vasospasm显示文摘 | Lee JY Huang DL Keep R | 2008 | J Neurosci Methods2008，2，： | 1 |
| 4 | Radiofrequency catheter ablation as a cure for idiopathic tachycardia of both left and right ventricular origin显示文摘 | Coggins DL Lee RJ Sweeney J | 1994 | J Am Coll Cardiol1994，23，： | 1 |
| 5 | Pulmonary alveolar proteinosis:high resolution CT,chest radiographic,and functional correlations显示文摘 | Lee KN Levin DL Webb WR | 1997 | Chest1997，111，： | 1 |
| 6 | Clinical Response of Pelvic andPara-aortic Lymphadenopathy to a Radiation Boost in the DefinitiveManagement of Locally Advanced Cervical Cancer显示文摘 | Rash DL Lee YC Kashefi A | 2013 | Int J RadiatOncol Biol Phys2013，187，2： | 1 |
| 7 | A rapid cold-hardening process in insects显示文摘 | Lee RE Chen C Denlinger DL | | 0，，4832： | 1 |
| 8 | Vectors of the 1 969 yellow fever epidemic on the Jos Plateau, Nigeria 显示文摘 | Lee VH Moore DL | 1972 | Bull World Health Organ1972，46，5： | 1 |
| 9 | Pulmonary Alveolar Proteinosis:High-resolution CT,Chest Rediographic,and Functional Correlation显示文摘 | Lee KN levin DL Webb WR | 1997 | Chest1997，111，5： | 1 |
| 10 | Mid to long-term results of re- vision total hip replacement in patients aged 50 years or younger 显示文摘 | Lee PT Lakstein DL Lozano B | 2014 | Bone Joint J2014，96，8： | 1 |
| 11 | Surgical outcome in patients with epilepsy with occult vascular malformations treated with lesionectomy 显示文摘 | Kraemer DL Griebel ML Lee N | 1998 | Epilepsia1998，39，6： | 1 |
| 12 | Failure to regulate TNFinduced NF-kappa B and cell death responses in A20-deficient mice显示文摘 | Lee EG Boone DL Chai S | 2000 | Science2000，289，5488： | 1 |
| 13 | Angiotensin Ⅱ hyperten sion is attenuated in interleukin-6 knockout mice显示文摘 | Lee DL Sturgis LC Labazi H | 2006 | Am J Physiol Heart Circ Physiol2006，290，3： | 1 |
| 14 | Radiofrequency catheter ablation as a cure for idiopathic tachycardia of both left and right ventricular origin 显示文摘 | Coggins DL Lee RJ Sweeney J | 1994 | J Am Coll Cardiol1994，23，： | 1 |
| 15 | Tau interacts with src-family non-receptor tyrosine kinases显示文摘 | Lee G Newman ST Gard DL Band H Panchamoorthy G | 1998 | J Cell Sci1998，111，21： | 1 |
| 16 | Pulmonary alveolar proteinosis:high-resolution CT,chest radiogrphic,and functional correlations显示文摘 | Lee KN Levin DL Webb WR | 1997 | Chest1997，11，7： | 1 |
| 17 | Measuring health in patients with cervi- cal and lumbosacral spinal disorders:is the 12 -item short form health survey a valid alternative for the 36 - item short form health survey? 显示文摘 | Lee CE Browell LM Jones DL | 2006 | Arch Phys Med Rehabil2006，89，5： | 1 |
| 18 | Prone position ventilation induces sustained improvement in oxygenation in patients with acute respiratory distress syndrome who have a large shunt显示文摘 | Lee DL Chiang HT Lin SL | 2002 | Crit Care Med2002，30，7： | 1 |
| 19 | Clopidogrel pharmacokinetics and pharmacodynamics vary widely despite exclusion or control of polymorphisms (CYP2C19, ABCB1, PON1), noncompliance, diet, smoking, co-medications (including proton pump in- hibitors) , and pre-existent variability in platelet func- tion显示文摘 | Frelinger AL Bhatt DL Lee RD | 2013 | J Am Coll Cardiol2013，61，8： | 1 |
| 20 | Clopidogrel pharmacokinetics and pharmacodynamics vary widely despite exclusion or control of polymorphisms(CYP2C19,ABCB1,PON1),noncompliance,diet,smoking,co-medications(including proton pump inhibitors),and pre-existent variability in platelet function显示文摘 | FRELINGER AL BHATT DL LEE RD | 2013 | J Am Coll Cardiol2013，61，8： | 1 |
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