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| 1 | Activation of human fibroblast-like synoviocytes by uric acid crystals in rheumatoid arthritis显示文摘Hyperuricemia-mediated uric acid crystal formation may cause joint inflammation and provoke the destruction of joints through the activation of inflammasome-mediated innate immune responses.However,the immunopathological effects and underlying intracellular regulatory mechanisms of uric acid crystal-mediated activation of fibroblast-like synoviocytes(FLS)in rheumatoid arthritis(RA)have not been elucidated.Therefore,we investigated the in vitro effects of monosodium urate crystals,alone or in combination with the inflammatory cytokines tumor-necrosis factor(TNF)-a or interleukin(IL)-1b,on the activation of human FLS from RA patients and normal control subjects and the underlying intracellular signaling mechanisms of treatment with these crystals.Monosodium urate crystals were able to significantly increase the release of the inflammatory cytokine IL-6,the chemokine CXCL8 and the matrix metalloproteinase(MMP)-1 from both normal and RA-FLS(all P,0.05).Moreover,the additive or synergistic effect on the release of IL-6,CXCL8 and MMP-1 from both normal and RA-FLS was observed following the combined treatment with monosodium urate crystals and TNF-a or IL-1b.Further experiments showed that the release of the measured inflammatory cytokine,chemokine and MMP-1 stimulated by monosodium urate crystals were differentially regulated by the intracellular activation of extracellular signal-regulated kinase and c-Jun N-terminal kinase pathways but not the p38 mitogen-activated protein kinase pathway.Our results therefore provide a new insight into the uric acid crystal-activated immunopathological mechanisms mediated by distinct intracellular signal transduction pathways leading to joint inflammation in RA. | Da P Chen Chun K Wong Lai S Tam Edmund K Li Christopher WK Lam | 2011 | Cellular & Molecular Immunology2011，8，6： | 5 |
| 2 | Anaerobic bacteria and intrahepatic stones: detections of Clostridium sp. and Bacteroides fragilis显示文摘To detect anaerobic bacteria Clostridium sp . and Bacteroides fragilis in intrahepatic stones by molecular genetic method Methods DNA was extracted from 59 stone samples and subjected to polymerase chain reaction (PCR) amplification targeting the 16S rRNA gene of Clostridium sp . and the glutamine synthetase gene of Bacteroides fragilis Single-strand conformational polymorphism (SSCP) analysis was performed to identify the Clostridium sp Results 16S rRNA gene sequences for Clostridium sp. were identified in 49 stones (83%, 49/59) The two most common groups were detected in 19 (41%) and 17 (37%) of the 46 samples using SSPC analysis, and 25/59 (42%) stones were tested positive for Bacteroides fragilis Conclusions Anaerobes such as Clostridium sp and Bacteroides fragilis present in intrahepatic stones and may play a role in stone formation PCR is a useful technique to detect fastidious pathogens, which are difficult to culture SSCP of PCR products is a rapid method in differentiating bacterial | LIU Yanlei Kan Lam Yajarayma J Tang Paul H Gumerlock Dong Ki Lee Myung Hwa Kim Sum P Lee Joseph SilvaJr Joseph W Leung | 2000 | Chinese Medical Journal2000，，9： | 2 |
| 3 | Analytic relation between bulk moduli and lattice constants显示文摘 | Cohen M L Martinez G | 1987 | Phys Rev B1987，35，17： | 1 |
| 4 | Framework of Success Criteria for Design Build Projects 显示文摘 | A P C Chan D Scott E W M Lam | 2002 | Journal of Management in Engineering2002，18，3： | 1 |
| 5 | Interdaily volatility in continuous order-driven market显示文摘 | Lam P H Tong W H | 1999 | Journal of Business Finance and Accounting1999，26，78： | 1 |
| 6 | Epidemiologieal features of supracondylar fractures of the humerus in Chinese children显示文摘 | Cheng J C Lam T P Maffulli N | 2001 | Pediatr Orthop B2001，10，： | 1 |
| 7 | Study of the destructive effect to inherent quality of Angelicae dahur icae radix(Bai Zhi)by sulfur fumigated process using chroma to grphic finger printing analysis显示文摘 | Wang X H Xie P S Lam Chris W K | 2009 | J Pharm Biomed Anal2009，49，5： | 1 |
| 8 | Optimisation of re- gional energy supply chain utilizing renewable: P-graph ap- proach显示文摘 | Lam H L Varbanov P S Kleme~ J J | 2010 | Computers and Chemical Engineering2010，34，5： | 1 |
| 9 | Modelling risk allocation decision in construction contracts 显示文摘 | Lam K C Wang D Lee P T K etal | 2007 | International Journal of Project Management2007，25，5： | 1 |
| 10 | Impact of mass spectrometry by MALDI-TOF MS for the rapid identification of aerobic and anaerobic bacteria of clinical importance显示文摘 | LEGARRAGA P MORAGA M LAM M | 2013 | Rev Chilena Infectol2013，30，2： | 1 |
| 11 | Acetylenes and related compounds in Cynareae 显示文摘 | Christensen L P Lam J | 1990 | Phytochemistry1990，29，9： | 1 |
| 12 | Orthogonal(g,f)-factorizations in networks显示文摘 | Lam P C B Liu Guizhen Li Guojun | 2000 | Networks2000，35，4： | 1 |
| 13 | Revising the nitrogen cycle in the Peruvian oxygen minimum zone 显示文摘 | Lam P Lavik G Jensen M M | 2009 | Proceeding of the National Academy (if Sciences2009，106，12： | 1 |
| 14 | Parents* knowledge and perspec-tive of optical methods for myopia control in children 显示文摘 | Cheung S W Lam C Cho P | 2014 | Op-tom Vis Sci2014，91，6： | 1 |
| 15 | Preparation of Cu2ZnSnS4 films by electrodeposition using ionic liquids显示文摘 | CHAN C P LAM H SURYA C | | 0，，02： | 1 |
| 16 | Acetylenes and related compounds in Heliantheae 显示文摘 | Christensen L P~ Lam J | 1991 | Phytochemistry1991，30，1： | 1 |
| 17 | Identificationand characterization of a new degradation product ofIrgarol 1051 in mercuric chloride-catalyzed hydrolysisreaction and in coastal waters显示文摘 | Lam K H Lam M H W Lam P K S | 2004 | Marine PollutionBulletin2004，49，： | 1 |
| 18 | Occurrence and distribution of polybrominated dipbenyl ethers (PBDEs) in the dissolved and suspended phases of the sea-surface microlayer and seawater in Hong Kong, China 显示文摘 | Wurl O Lam P K Obbard J P | 2006 | Chemosphere2006，65，9： | 1 |
| 19 | Optimized synthesis of polyglutaraldehyde nanoparticles using central composite design 显示文摘 | McLeod A D Lam F C Gupta P K | 1988 | J Pharm Sci1988，77，8： | 1 |
| 20 | Nonlinear transient behavior of a sliding system with frictionally excited thermoelastic instability显示文摘 | LAM K B BAHKALI E A | 2001 | Journal of Tribology2001，123，： | 1 |
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