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| 1 | A high-density, multi-parental SNP genetic map on apple validates a new mapping approach for outcrossing species显示文摘Quantitative trait loci(QTL)mapping approaches rely on the correct ordering of molecular markers along the chromosomes,which can be obtained from genetic linkage maps or a reference genome sequence.For apple(Malus domestica Borkh),the genome sequence v1 and v2 could not meet this need;therefore,a novel approach was devised to develop a dense genetic linkage map,providing the most reliable marker-loci order for the highest possible number of markers.The approach was based on four strategies:(i)the use of multiple full-sib families,(ii)the reduction of missing information through the use of HaploBlocks and alternative calling procedures for single-nucleotide polymorphism(SNP)markers,(iii)the construction of a single backcross-type data set including all families,and(iv)a two-step map generation procedure based on the sequential inclusion of markers.The map comprises 15417 SNP markers,clustered in 3 K HaploBlock markers spanning 1267 cM,with an average distance between adjacent markers of 0.37 cM and a maximum distance of 3.29 cM.Moreover,chromosome 5 was oriented according to its homoeologous chromosome 10.This map was useful to improve the apple genome sequence,design the Axiom Apple 480 K SNP array and perform multifamily-based QTL studies.Its collinearity with the genome sequences v1 and v3 are reported.To our knowledge,this is the shortest published SNP map in apple,while including the largest number of markers,families and individuals.This result validates our methodology,proving its value for the construction of integrated linkage maps for any outbreeding species. | Erica A Di Pierro Luca Gianfranceschi Mario Di Guardo Herma JJ Koehorst-van Putten Johannes W Kruisselbrink Sara Longhi Michela Troggio Luca Bianco Hélène Muranty Giulia Pagliarani Stefano Tartarini Thomas Letschka Lidia Lozano Luis Larisa Garkava-Gustavsson Diego Micheletti Marco CAM Bink Roeland E Voorrips Ebrahimi Aziz Riccardo Velasco François Laurens W Eric van de Weg | 2016 | Horticulture Research2016，3，1： | 3 |
| 2 | Molecular cloning of the cDNA encoding laccase from Pycnoporus cinnabarinus I 937 and expression in Pichia pastoris 显示文摘 | Otterbeinl L Recordl E Longhi S | 2000 | Eur J Biochem2000，267，： | 2 |
| 3 | Development and experimential validation of an adaptive extended Kalman filter for localization of mobile robots显示文摘 | JETTO L LONGHI S VENTURINI G | 1999 | IEEE Transactions on Robotics and Automation1999，15，2： | 1 |
| 4 | Effects of hyperoxia on brain tissue oxygen tension in cerebral focal lesions 显示文摘 | Longhi L Valeriani V Rossi S | 2002 | Acta Neurochir2002，81，： | 1 |
| 5 | Stem cell transplantation as atherapeutic strategy for traumatic brain injury 显示文摘 | Longhi L Zanier ER Royo N | 2005 | Transpl Immunol2005，15，： | 1 |
| 6 | Genetically modified NT2N human neuronal cells mediate long-term gene expression as CNS grafts in vivo and improve functional cognitive outcome following experimental traumatic brain injury显示文摘 | WATSON D J LONGHI L LEE E B | 2003 | J Neuropathol Exp Neurol2003，62，4： | 1 |
| 7 | Stem cell transplantation as a therapeutic strategy for traumatic brain injury显示文摘 | Longhi L Zanier ER Royo N | 2005 | Transpl Immunol2005，15，2： | 1 |
| 8 | Ex vivo gene therapy using targeted engraftment of NGF-expressing human NT2N neurons attenuates cognitive deficits following traumatic brain injury in mice显示文摘 | LONGHI L WATSON D J SAATMAN K E | 2004 | J Neurotrauma2004，21，12： | 1 |
| 9 | Influence of lactoferrin on the entry process of Escherichia coli I-IB 101 (pRI203) in HeLa cells显示文摘 | LONGHI C CONTE M P SEGANTI L | 1993 | Med Microbiol Immunol1993，182，1： | 1 |
| 10 | An empirical thermal history of the Earth's upper mantle显示文摘 | Abbott D Burgess L Longhi J | 1994 | Journal of Geophysi- cal Research1994，99，13： | 1 |
| 11 | Intracranial pressure monitoring for traumatic brain injury: available evidence and clinical implications 显示文摘 | Stocchetti N Longhi L Zanier E R | 2008 | Minerva Anestesiol2008，74，5： | 1 |
| 12 | Motor and cognitive function evaluation following experimental traumatic brain injury 显示文摘 | Fujimoto S T Longhi L Saatman K E | 2004 | Neurosci Biobehav Rev2004，28，4： | 1 |
| 13 | Molecular cloning of the cDNA encoding laccase from Pycnoporus cinnabarinus 1-937 and expression in Pichia pastoris显示文摘 | Otterbein L Record E Longhi S | 2000 | Eur J Biochem2000，267，6： | 1 |
| 14 | Development and experimental validation of an adaptive extended Kalman filter for the localization of mobile robots 显示文摘 | Longhi S Venturini G | 1999 | IEEE Trans on Robot and Automation1999，15，2： | 1 |
| 15 | Development and experimental validation of an adaptive extended Kalman filter for the localization of mobile robots显示文摘 | Longhi S Venturini G | 1999 | IEEE Transaction on Robotics and Automation1999，15，2： | 1 |
| 16 | Interaction of nitro- gen status and UVB sensitivity in a temperate phytoplankton as- semblage显示文摘 | BOUCHARD J N LONGHI M L ROY S | 2008 | J Exp Mar Biul Ecnl2008，359，1： | 1 |
| 17 | Preparation and characterization of aminated carbon from a single-step reaction显示文摘 | LONGHI M BERTACCHE V BIANCHI C L | 2006 | Chemistry of Materials2006，18，17： | 1 |
| 18 | Intracranial pressure monitoring for traumatic brain injury: available evidence and clinical implications显示文摘 | Stocchetti N Longhi L Zanier ER | 2008 | Minerva Anestesiol2008，74，5： | 1 |
| 19 | Ex vivo gene therapy using targeted engraftment of NGF- expressing human NT2N neurons attenuates cognilive deficits following traumatic brain injury in mice显示文摘 | Longhi L Watson DJ Saatman KE | 2004 | J Neurotrauma2004，21，12： | 1 |
| 20 | Hyperoxia in head injury:therapeutic tool显示文摘 | Stocchetti N | 2004 | Curr Opin Crit Care2004，10，2： | 1 |
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