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| 1 | Enhancement of transfection efficiency for HeLa cells via incorporating arginine moiety into chitosan显示文摘Arginine-rich peptides have attracted considerable attention due to their distinct internalization mechanism. It was reported that arginine and guanidino moieties were able to translocate through cell membranes and played a critical role in the process of membrane permeation. In this work, arginine was conjugated to the backbone of chitosan to form a novel chitosan derivative, arginine modified chitosan (Arg-CS). Arg-CS/DNA complexes were prepared according to the method of coacervation process. The physicochemical properties of Arg-CS and Arg-CS/DNA complexes were characterized and the transfection activity and efficiency mediated by Arg-CS/DNA complexes were investigated taking HeLa cells as target cells. Arg-CS was characterized by FTIR and 13C NMR. Arg-CS/DNA polye- lectrolyte complexes were investigated by agarose gel retardation, dynamic light scattering (DLS) and atomic force microscopy (AFM). The results revealed that the Arg-CS/DNA complexes started to form at N/P ratio of 2:1, and the size of particles varied from 100 to 180 nm. The cytotoxicity of Arg-CS and their complexes with plasmid DNA were determined by MTT assay for HeLa cells, and the results suggested that Arg-CS/DNA complexes were slightly less toxic than Arg-CS. Moreover, the derivative alone and their complexes showed significantly lower toxicity than PEI and PEI/DNA complexes, respectively. Taking HeLa cells as target cells and using pGL3-control as reporter gene, the luciferase expression mediated by Arg-CS was greatly enhanced to about 100 folds compared with the luciferase expression mediated by chitosan at different pH media. These results suggest that Arg-CS is a promising candi- date as a safe and efficient vector for gene delivery and transfection. | ZHU DunWan ZHANG HaiLing BAI JinGen LIU WenGuang LENG XiGang SONG CunXian YANG Jian LI XiaoWei JIN Xu SONG LiPing LIU LanXia LI XiuLan ZHANG Yang YAO KangDe | 2007 | Chinese Science Bulletin2007，52，23： | 4 |
| 2 | Influence of Charge Density and Chain Length on the Interaction between Organic Anion and Montmorillonite显示文摘Anionic surfactant sodium dodecyl sulfate (SDS), cationic surfactant cetyl trimethyl ammonium bromide (CTAB) and acrylic acid (AA) were introduced as molecular models to study the interaction between montmorillonite and organic molecules with different charge or chain length. The compounds were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and 13C nuclear magnetic resonance (13C NMR). The results show organic anion could interact strongly with montmorillonite, even the molecules could intercalate into the layers of MMT. | 郑俊萍 LI Jian HAO Hui YAO Kangde | 2013 | Journal of Wuhan University of Technology(Materials Science)2013，28，1： | 3 |
| 3 | The multiple interactions between growth factors and microenvironment in vivo显示文摘Cell, growth factors and extracellular ma- trices (ECMs) coexist in a dynamic tissue envi- ron- ment. A knowledge of multiple interactions among them is highly important for effectively raising the biological activities of growth factors, regulating cell life cycle, designing and preparing exogenous mat- rices to control growth factors release in tissue or organ regeneration by engineering means. This pa- per addresses the characteristics and functions of growth factors, interactions between growth factors and ECMs, the manners and correlative signaling of growth factors acting on cells, and briefly summarizes the biomimetic requisites for controlled release mat- rices, hoping to provide a useful reference for co- rrelative research in tissue engineering. | BO Jin'gen YANG Jian ZHU Dunwan LI Junjie YAO Kangde | 2006 | Chinese Science Bulletin2006，51，7： | 2 |
| 4 | Preparation and mineralization of PLGA/Gt electrospun fiber mats显示文摘Poly(lactic-co-glycolic acid)(PLGA)/gelatin(Gt) ultrafine composite fibers were fabricated via electro-spinning.The effect of gelatin on the morphology and tensile property of the electrospun fiber mats was investigated.Mineralization was carried out in 10×simulated body fluid(10SBF).The deposited calcium phosphate(CaP) was identified by scanning electron microscopy(SEM),energy dispersive spectroscopy(EDS),X-ray diffraction(XRD) and Fourier transform infrared spectroscopy(FTIR).Results indicated that the average diameter of PLGA/Gt electrospun fibers was much smaller than that of PLGA fibers and the addition of gelatin increased the tenacity of wet mats.Two types of calcium phosphate deposits with different molar ratios of Ca/P were obtained when the electrospun PLGA/Gt mat was incubated in 10SBF.CaP crystals nucleated and grew faster on PLGA/Gt fiber mats rather than on PLGA membranes.'Honeycomb-like' crystals grew on the surface of PLGA/Gt electrospun fibers.After 4 h,the surface of the fibers was entirely coated with these crystals. | ZHAO Jin ZHAO YuPing ZHANG Wei YUAN XiaoYan YAO KangDe | 2009 | Chinese Science Bulletin2009，54，8： | 2 |
| 5 | Functional display of foreign protein on surface of coli using N-terminal domain of ice nucleation protein 显示文摘 | LI L KANGD G CHA H J | 2004 | Biotechnology and Bioengineering2004，85，2： | 1 |
| 6 | Effect of nano- and micro-hydroxyapatite/chitosan-gelatin network film on human gastric cancer cells显示文摘 | Junjie Li Yuji Yin Fanglian Yao Lili Zhang Kangde Yao | 2008 | Materials Letters2008，，17： | 1 |
| 7 | Study on the synthesis and application of starch-graft-poly (AM-co-DADMAC) by using a complex initiation system of CS-KPS 显示文摘 | LUA Shaojie LINB Songbai YAOB Kangde | 2004 | Starch/Stake2004，56，： | 1 |
| 8 | SignificanceofacutemultiplebraininfarctionondiffusionGweightedimaging显示文摘 | ROHJK KANGD W LEESH etal | 2000 | Stroke2000，31，3： | 1 |
| 9 | Rat osteoblast functions on the O-carboxymethy chitosan-modified poly(D,L-lactic acid) surface显示文摘 | CAI Kaiyong YAO Kangde LI Zhi | 2001 | J Biomater Sci Polym Ed2001，12，12： | 1 |
| 10 | Poly (DL-lactic acid) surfaces modified by silk fibroin: ettects on the culture of osteoblast in vitro显示文摘 | Kaiyong Cai Kangde Yao Songbai Lin Zhiming Yang Xiuqiong Li Huiqi Xie Tingwu Qing and Laibao Gao | 2002 | Biomaterials2002，23，4： | 1 |
| 11 | Chitosan and its derivatives-a promising non-viral vector for gene transfection显示文摘 | Liu Wenguang Yao Kangde | 2002 | Journal of Controlled Release2002，83，： | 1 |
| 12 | Evaluation of porous collagen membrane in guided tissue regeneration 显示文摘 | Qiqing Zhang Kangde Yao Lingrong Liu etal | 1999 | Artificial Cell Blood Substitutes and Immobilization Biotechnology1999，27，3： | 1 |
| 13 | Rat osteoblast functions on the o-carboxymethyl chitosan-modified poly(D,L-lactic acid) surface显示文摘 | Kaiyong Cai Kangde Yao Zhi Li Zhiming Yang Xiuqiong Li | 2001 | Journal of Biomaterials Science Polymer Edition2001，，12： | 1 |
| 14 | A new approach to analysis and optimizationof evaporative cooling system ( I ) : Theory 显示文摘 | Chen Qun Yang Kangding Wang Moran Pan Ning Guo Zengyuan | 2010 | Energyy2010，35，： | 1 |
| 15 | pH-- dependent hydrolysis and drug release of chitosan/polyether interpenetrating polymer network hydrogel显示文摘 | Kangde Yao Tao Peng Qiqing Zhang | 1994 | Polymer international1994，，34： | 1 |
| 16 | Visualization of coronary atherosclerotic plaques in patients using optical coherence tomography: Comparison with intravascular ultrasound 显示文摘 | JANGIK BOUMABE KANGD H etal | 2002 | J Am Coll Cardiol2002，39，： | 1 |
| 17 | Study on Synthesis and Application of Starch-graft Poly(AM-co-DADMAC)by Using a Complex Initiation System of CS-KPS显示文摘 | Lu Shaojie Lin Songbai Yao Kangde | 2004 | Starch/Starke2004，56，： | 1 |
| 18 | 显示文摘 | Liu Haifeng Yin Yuli Yao Kangde | 2004 | Biomaterials2004，25，： | 1 |
| 19 | Successful treatment of Boerhaave's syndromewith endoscopic insertion of a self-expandable metallic stent: report of three cases and a review of the literature显示文摘 | CHUNG MG KANGD H PARK DK etal | 2001 | Endoscopy2001，33，： | 1 |
| 20 | A new approach to analysis and optimization of evaporative cooling system I : Theory 显示文摘 | Qun Chen Kangding Yang Moran Wang | 2010 | Energy2010，35，6： | 1 |
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