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| 1 | Effect of Electro-acupuncture on Vasomotor Symptoms in Rats with Acute Cerebral Infarction Based on Phosphatidylinositol System显示文摘Objective:To investigate the effect of electro-acupuncture(EA)on vasomotor symptoms in rats with acute cerebral infarction,by observing the changes in the expression of factors related to the phosphatidylinositol(PI)system.Methods:Forty-two Wistar rats were randomly divided into 3 groups by a random number table:the control group(n=6),the model group(n=18)and the EA group(n=18).The EA group was given EA treatment at Shuigou(GV 26)instantly after modeling with middle cerebral artery occlusion(MCAO)method,while the model and control groups were not given any treatment.The degrees of neurological deficiency were evaluated using neurological severity scores(NSS)and the brain blood flow was evaluated by a laser scanning confocal microscope.Western blot analysis was conducted to detect the expression levels of G-protein subtype(Gq)and calmodulin(CaM).Competition for protein binding was conducted to detect the expression level of inositol triphosphate(IP3).Thin layer quantitative analysis was conducted to detect the expression level of diacylglycerol(DAG).The expression level of intracellular concentration of free calcium ion([Ca^(2+)]i)was detected by flow cytometry.Results:The NSS of the model group was significantly higher than the control group at 3 and 6 h after MCAO(P<0.01),while the EA group was significantly lower than the model group at 6 h(P<0.01).The cerebral blood flow in the model group was significantly lower than the control group at 1,3 and 6 h after MCAO(P<0.01),while for the EA group it was remarkably higher than the model group at the same time points(P<0.01).The expressions of Gq,CaM,IP3,DAG and[Ca^(2+)]i in the model group were significantly higher than the control group(P<0.05 or P<0.01),and those in the EA group were significantly lower than the model group at the same time points(P<0.05 or P<0.01).Conclusion:EA treatment at GV 26 can effectively decrease the over-expression of related factors of PI system in rats with acute cerebral infarction,improve cerebral autonomy movement,and alleviate cerebral vascular spasm. | LI Jing HE Ying DU Yuan-hao ZHANG Min Rainer Georgi Bernhard Kolberg SUN Dong-wei MA Kun LI Yong-feng ZHANG Xue-zhu | 2022 | Chinese Journal of Integrative Medicine2022，28，2： | 7 |
| 2 | Molecular Mechanism of Electroacupuncture Regulating Cerebral Arterial Contractile Protein in Rats with Cerebral Infarction Based on MLCK Pathway显示文摘Objective To explore the effect of electroacupuncture(EA)intervention on the vasoconstriction of cerebral artery smooth muscle cells after cerebral infarction.Methods Male Wistar rats were randomly divided into 3 groups by a random number table:the model group(n=24),the EA group(n=24),and the normal group(n=6).The model and the EA groups were divided into different time subgroups at 0.5,1,3,and 6 h after middle cerebral artery occlusion(MCAO),with 6 rats in each subgroup.MCAO model was established using intraluminal suture occlusion method.The EA group was given EA treatment at acupoint Shuigou(GV 26)instantly after MCAO for 20 min.The contents of cerebrovascular smooth muscle MLCK,the 3 subunits of myosin light chain phosphatase(MLCP)MYPT1,PP1c-δand M20,as well as myosin-ATPase activity were detected using immunohistochemistry and Western blotting.Results The overall expression level of the MYPT1 and PP1c-δin the model group was significantly higher(P<0.01).After EA intervention,the 0.5 h group expression level was close to that of the normal group(P>0.05),and the other subgroups were still significantly higher than the normal group(P<0.01).After EA intervention,the expression level of each subgroup was significantly lower than the corresponding model group.There was a significant difference between the 0.5 and 1 h subgroups(P<0.01),while a difference was also observed between the 3 and 6 h subgroups(P<0.05).The dynamic change rule gradually increased with the prolongation of infarction time within 6 h after infarction.Conclusion EA intervention can inhibit contraction of cerebral vascular smooth muscle cells and regulate smooth muscle relaxation by regulating MLCK pathway. | LI Jing ZHANG Min HE Ying DU Yuan-hao ZHANG Xue-zhu Rainer Georgi Bernhard Kolberg Xu Yan-long | 2023 | Chinese Journal of Integrative Medicine2023，29，1： | 2 |
| 3 | Development of a fast multiresidue method for the determination of pesticides in dry samples(wheat grains,flour and bran)using Qu ECh ERS based method and GCMS显示文摘 | KOLBERG D I PRESTES O D ADAIME M B | 2011 | Food Chemistry2011，125，4： | 1 |
| 4 | Development of a fast multiresidue method for the determination of pesticides in dry samples(wheat grains,flour and bran)using Qu ECh ERSbased method and GC–MS显示文摘 | Kolberg D I Prestes O D Adaime M B | 2011 | Food Chemistry2011，125，14： | 1 |
| 5 | Mutationspectrum in children with primary hemophagocytic lym-phohistiocytosis:Molecular and functional analyses ofPRF1,UNC13D,STX11,and RAB27A显示文摘 | Zur Stadt U Beutel K Kolberg S | 2006 | HumMutat2006，27，： | 1 |
| 6 | Mutation spectrum in children with primary hemophagocytic lymphohistiocytosis:molecular and functional analyses of PRF1,UNC13D,STX11and RAB27A显示文摘 | Zur Stadt U Beutel K Kolberg S | 2006 | Human Mutation2006，27，： | 1 |
| 7 | Development and independent laboratory validation of a simple method for the determination of paraquat and diquat in potato, cereals and pulses显示文摘 | Kolberg D I Mack D Anastassiades M | 2012 | Anal Bioanal Chem2012，404，8： | 1 |
| 8 | Feature interaction:a critical review and considered forecast显示文摘 | Kolberg M Magill E H | 2003 | Computer Networks2003，41，1： | 1 |
| 9 | Low expression of MRP1/GS-X pump ATPase in lymphocytes of Walker 256 tumour-bearing rats is associated with cyclopentenone prostaglandin accumulation and cancer immunodeficiency显示文摘 | Kolberg A Rosa TG Puhl MT | 2006 | Cell Biochem Funct2006，24，1： | 1 |
| 10 | Connexin43 acts as a color- ectal cancer tumor suppressor and predicts disease outcome 显示文摘 | Sirnes S Braun J Kolberg M | 2012 | Int J Cancer2012，131，3： | 1 |
| 11 | At least five related but distinct hepatitis C viral genotypes exist 显示文摘 | Cha TA Kolberg J Kuo G | 1992 | Proc Nad Acad Sci USA1992，89，： | 1 |
| 12 | Mutation spectrum in children with primary hemophagocytic lymphohistiocytosis: molecular and functional analyses of PRF1, UNC13D, STX 11, and RAB27A 显示文摘 | Zur SU Beutel K Kolberg S | 2006 | Hum Mutat2006，27，1： | 1 |
| 13 | Human embryo cloning reported显示文摘 | Kolberg R | 1993 | Science1993，262，： | 1 |
| 14 | Application of six hepatitis C virus subtyping systems to sera of patients with chronic hepatitis C in the United States显示文摘 | Lau JY Mizokami M Kolberg JA | 1995 | J Infect Dis1995，171，： | 1 |
| 15 | Precise modeling framework for short-channel double-gate and gate-all-around MOSFETs 显示文摘 | BORLI H KOLBERG S FJELDLY T | 2008 | IEEE Transactions on Electron Devices2008，55，10： | 1 |
| 16 | The effect of exogenous estradiol benzoate and altrenogest on uterine and ovarian blood flow during the estrous cycle in mares显示文摘 | Bollwein H Kolberg B Stolla R | 2004 | Theriogenology2004，61，6： | 1 |
| 17 | Development of tunable close match LED solar simulator with extended spectral range to UV and IR显示文摘 | Kolberg D Schubert F Lontke N | 2011 | Energy Procedia2011，8，： | 1 |
| 18 | Mutation spectrum in children with primary hemophagocytic lymphohistiocytosis: molecular and functional analyses of PRF1, UNC13D, STXII, and RAB27A 显示文摘 | Zur Stadt U Beutel K Kolberg S | 2006 | Hum Mutat2006，27，1： | 1 |
| 19 | Low expression of MRP1/GS- X pump ATP ase in lymphocytes of Walker 256 tumour-bearing rats is associated with cyclopentenone prostaglandin accumulation and cancer immunodeficiency 显示文摘 | Kolberg A Rosa TG Puhl MT | 2006 | Cell Biochem Funct2006，24，1： | 1 |
| 20 | Streptococcus pneumoniae enolase is important for plasminogen binding despite low abundance of enolase protein on the bacterial cell surface 显示文摘 | Kolberg 1 Aase A Bergmann S | 2006 | Microbiology2006，152，5： | 1 |
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