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| 1 | Characterization of modified mesoporous silica nanoparticles as vectors for siRNA delivery显示文摘Gene therapy using siRNA molecules is nowadays considered as a promising approach. For successful therapy, development of a stable and reliable vector for siRNA is crucial. Non-viral and non-organic vectors like mesoporous silica nanoparticles(MSN) are associated with lack of most viral vector drawbacks, such as toxicity, immunogenicity, but also generally a low nucleic acid carrying capacity. To overcome this hurdle, we here modified the pore walls of MSNs with surface-hyperbranching polymerized poly(ethyleneimine)(hbPEI), which provides an abundance of amino-groups for loading of a larger amount of siRNA molecules via electrostatic adsorption. After loading, the particles were covered with a second layer of pre-polymerized PEI to provide better protection of siRNA inside the pores, more effective cellular uptake and endosomal escape. To test the transfection efficiency of PEI covered si RNA/MSNs, MDA-MB 231 breast cancer cells stably expressing GFP were used. We demonstrate that PEI-coated si RNA/MSN complexes provide more effective delivery of si RNAs compared to unmodified MSNs. Thus, it can be concluded that appropriately surface-modified MSNs can be considered as prospective vectors for therapeutic siRNA delivery. | Anna Slita Anna Egorova Eudald Casals Anton Kiselev Jessica M.Rosenholm | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，6： | 2 |
| 2 | Laparoscopic myomectomy in treatment of large myomas 显示文摘 | ADAMIAN LV KULAKOV VI KISELEV SI | 1996 | J Am Asscc Gynecol Laparosc1996，3，4： | 1 |
| 3 | Is the brain codex a fractal? 显示文摘 | Kiselev VG Hahn KR Auer DP | 2003 | Neuroimage2003，20，3： | 1 |
| 4 | Intrinsic localized spin -wave modes in antiferromagnetic chains with single -ion easy - axis anisotropy显示文摘 | LAI R KISELEV S A SIEVERS A J | 1996 | Phys Rev B1996，54，12： | 1 |
| 5 | Diagnosis and treatment of uterine septum显示文摘 | Adamian L V Kulakov V I Kiselev S I | 1996 | J Am Assoc Gynecol Laparosc1996，，4： | 1 |
| 6 | Size of a human serum albumin molecule in solution显示文摘 | Kiselev M A Gryzunov IuA Dobretsov G E | 2001 | Biofizika2001，46，： | 1 |
| 7 | 查看详情显示文摘 | Kiselev E A Cherepanov V A | | 0，，01： | 1 |
| 8 | Clinical trials of the new skin test Diaskintest for the diagnosis of tuberculosis显示文摘 | Kiselev VI Baranovskii PM Rudykh IV | 2009 | Probl Tuberk Bolezn Legk2009，，2： | 1 |
| 9 | Blood lipids and the incidence of hyperlipidemia in pilots显示文摘 | Bugrov SA Kiselev RK Beleda RV | 1986 | Kosm Biol Avi- akosm Med1986，20，3： | 1 |
| 10 | 7-azainde- noisoquinolines as topoisomerase I inhibitors and po- tential anticancer agents 显示文摘 | Kiselev E DeGuire S Morrell A | 2011 | J Med Chem2011，54，17： | 1 |
| 11 | Thermodynamic properties of R32 + R134a and R125 + R32 mixtures in and beyond the critical region显示文摘 | Kiselev S B Huber M L | 1998 | Int J Refrig1998，21，1： | 1 |
| 12 | Azainde- noisoquinolines as topoisomerase I inhibitors and po- tential anticancer agents: A systematic study of struc- ture-activity relationships 显示文摘 | Kiselev E Agama K Pommier Y | 2012 | J Med Chem2012，55，4： | 1 |
| 13 | Expression of calciumdependent protein kinase (CDPK) gens under abicrdc stress conditions in wild-growing grapevine Vitis amurensis显示文摘 | DUBROVINA A S KISELEV K V KHRISTENKO V S | 2013 | J Plant Physiol2013，170，17： | 1 |
| 14 | CD- PK gene expression in salt tolerant rolB and rolC transformed cell cultures of Panax ginseng显示文摘 | Kiselev K V Grishchenko O V Zhuravlev Y N | 2010 | Biolo- gia Plantarum2010，54，： | 1 |
| 15 | Optimiza- tion of the lactam side chain of 7-azaindenoisoquino-line topoisomerase I inhibitors and mechanism of ac- tion studies in cancer cells显示文摘 | Kiselev E Sooryakumar D Agama K | 2014 | J Med Chem2014，57，4： | 1 |
| 16 | CDPK gene expression in somatic embryos of Panax ginseng expressing rolC显示文摘 | Kiselev K V Turlenko A V Zhuravlev Y N | 2009 | Plant Cell Tiss Organ Cult2009，99，： | 1 |
| 17 | Hydraulic hammer drilling technology: Developments and capabil- ities 显示文摘 | Melamed Y Kiselev A Gelfgat M | 2000 | Journal of Energy Resources Technology2000，122，1： | 1 |
| 18 | 显示文摘 | Kiselev S B | 1997 | Fluid PhaseEuqilibria1997，128，： | 1 |
| 19 | Laboratory studies and numerical simulations of cloud droplet formation under realistic supersaturation conditions 显示文摘 | Stratmann F Kiselev A Wurzler S | 2004 | Journal of Atmospheric And Oceanic Technology2004，21，： | 1 |
| 20 | Perspectives for production and application of resveratrol显示文摘 | Kiselev KV | 2011 | App Microb & Biotech2011，90，： | 1 |
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