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| 1 | Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases显示文摘AIM To establish a relevant animal model ofhuman gastrointestinal cancer, which can beused for repetitive investigations, so as toimprove our understanding and management ofcarcinogenesis and cancer metastasis.METHODS Intact tissues of human colorectaland pancreatic cancers were transplanted innude mice. The biological characteristics of theoriginal and the corresponding transplantedtumors were investigated by HE staining, PASstaining and immunostaining. The metastases inthe livers and lungs of nude mice wereinvestigated by immunostaining withbiotinylated mab KL-1 and by RT-PCR using CK20specific primers.RESULTS There were totally 9 of 16 surgicalspecimens growing in nude mice subcutaneouslyand/.or orthotopically (4 of 6 colorectal and 5 of10 pancreatic cancer). Tumor cell content of thespecimens and freezing of tissue specimens areimportant factors influencing the growth oftransplanted tumor. In the group of fresh tumortissues with greater than 50% tumor cellcontent, the success rate of the transplantationwas 100% (3 cases of pancreatic cancer and 3cases of colorectal cancer). The orthotopicallytransplanted tumors resemble the original tumormorphologically and biologically, including TAAexpression such as CEA byimmunohistochemistry, and CEA level in theserum of mice. Ki-67 labeling index and theexpression of TAA especially K-ras, 17-lA andRA-96, are associated with the potential of tumorgrowth in nude mice. Micrometastases in thelungs and livers of tumor bearing mice can bedetected by immunostaining with biotinylatedmab KL-1 and CK20-specific RT-PCR.CONCLUSION An orthotopic transplantationmodel for human colon and pancreatic cancer innude mice has been set up. We have alsoestablished sensitive detection methods withCK-immunohistochemistry and CK20-RT-PCR tostudy xenotransplanted human cancer and itsmetastatic cancer cells in the liver and lung ofnude mice. This study may be helpful inunderstanding the mechanism of cancermetastasis and in developing new diagnosticmethods and therapeutic strategies formetastases including micrometastases. | Jun Hui Cui~1 Uwe Krueger~2 Doris Henne-Bruns~2 Bemd Kremer~2 Holger Kalthoff~2 ~1Department of General Surgery,First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China ~2Department of General Surgery,Christian-Albrechts-University,Kiel,GermanyDr.Jun Hui Cui graduated from Zhejiang Medical University in 1984,earned master degree in 1990,studied in the Surgical Department of Kiel University and worked in the Lab of Molecular Oncology of Kiel University from 1994-1997achieved M.D.from Kiel University.Germany,now associate professor of surgery,specialized in colorectal oncology.Adviser of graduated student for master degree,having 20 publications published in key Chinese or English journals. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 2 | Rock-weathering by lichens in Antarctic: patterns and mechanisms显示文摘Saxicolous species of lichens are able to induce and accelerate weathering of their rock substrate, and effects of lichens on substrate can be attributed to both physical and chemical causes. This paper is focused on biotic weathering actions of epilithic and endolithic species on the different rock types (sandstones and volcanogenic rocks) in Antarctica. The patterns, mechanisms, processes and neoformations of rock-weathering resulting from lichen colonization are expounded in detail. Furthermore, it is pointed out that, for a better understanding of the impacts of lichens on environments, the studies on the rate of biotic weathering and the comprehensive involvement of the lichen effects on weathering of natural rocks remain to be carried out in Antarctica. | CHEN Jie1, Hans-Peter Blume2(1. Institute of Soil Science, CAS, Nanjing 210008, China 2. Institute of Plant Nutrition and Soil Science, University of Kiel, D-24098 Kiel, Germany) | 2002 | Journal of Geographical Sciences2002，12，4： | 8 |
| 3 | Noninvasive methods for prediction of esophageal varices in pediatric patients with portal hypertension显示文摘AIM: To evaluate clinical and laboratory parameters for prediction of bleeding from esophageal varices (EV) in children with portal hypertension. METHODS: Retrospective study of 103 children (mean age: 10.1 ± 7.7 years), 95.1% with intrahepatic portal hypertension. All patients had no history of bleeding and underwent esophagogastroduodenoscopy for EV screening. We recorded variceal size (F1, F2 and F3), red-color signs and portal gastropathy, according to the Japanese Research Society for Portal Hypertension classification. Patients were classified into two groups: with and without EV. Seven noninvasive markers were evaluated as potential predictors of EV: (1) platelet count; (2) spleen size z score, expressed as a standard deviation score relative to normal values for age; (3)platelet count to spleen size z score ratio; (4) platelets count to spleen size (cm) ratio; (5) the clinical prediction rule (CPR); (6) the aspartate aminotransferase to platelet ratio index (APRI); and (7) the risk score. RESULTS: Seventy-one children had EV on first endoscopy. On univariate analysis, spleen size, platelets, CPR, risk score, APRI, and platelet count to spleen size z score ratio showed significant associations. The best noninvasive predictors of EV were platelet count [area under the receiver operating characteristic curve (AUROC) 0.82; 95%CI: 0.73-0.91], platelet: spleen size z score (AUROC 0.78; 95%CI: 0.67-0.88), CPR (AUROC 0.77; 95%CI: 0.64-0.89), and risk score (AUROC 0.77; 95%CI: 0.66-0.88). A logistic regression model was applied with EV as the dependent variable and corrected by albumin, bilirubin and spleen size z score. Children with a CPR < 114 were 20.7-fold more likely to have EV compared to children with CPR > 114. A risk score > -1.2 increased the likelihood of EV (odds ratio 7.47; 95%CI: 2.06-26.99). CONCLUSION: Children with portal hypertension with a CPR below 114 and a risk score greater than -1.2 are more likely to have present EV. Therefore, these two tests can be helpful in selecting children for endoscopy. | Marina Rossato Adami Cristina Targa Ferreira Carlos Oscar Kieling Vania Hirakata Sandra Maria Gonalves Vieira | 2013 | World Journal of Gastroenterology2013，19，13： | 4 |
| 4 | Adjunctive MSCs enhance myelin formation by xenogenic oligodendrocyte precursors transplanted in the retina显示文摘 | Aileen Arriola Mary E Kiel Yufang Shi Randall D McKinnon | 2010 | Cell Research2010，20，6： | 3 |
| 5 | Determination of tectonic background for sedimentary basin from sandstone geochemistry显示文摘The chemical composition of sedimentary rocks is mainly controlled by source rocks. Through examining chemical composition of terrigenous rocks, the tectonic evolution of a sedimentary basin can be modeled. The Turpan Basin belongs to a continental sedimentary basin and its source rocks are derived from the upper continental crust. | SHAO Lei 1, K. Stattegger 2 and LI Wenhou 3 1. Laboratory of Marine Geology, Tongji University, Shanghai 200092, China 2. Geological and Paleontological Institute, University of Kiel, 24118 Kiel, Germany 3. Department of Geology, Northwest Unive | 1998 | Chinese Science Bulletin1998，43，14： | 3 |
| 6 | Association of types of diabetes and insulin dependency on birth outcomes显示文摘BACKGROUND Diabetes rates among pregnant women in the United States have been increasing and are associated with adverse pregnancy outcomes.AIM To investigate differences in birth outcomes(preterm birth,macrosomia,and neonatal death)by diabetes status.METHODS Cross-sectional design,using linked Missouri birth and death certificates(singleton births only),2010 to 2012(n=204057).Exposure was diabetes non-diabetic,pre-pregnancy diabetes-insulin dependent(PD-I),pre-pregnancy diabetes-non-insulin dependent(PD-NI),gestational diabetes-insulin dependent(GD-I),and gestational diabetes-non-insulin dependent(GD-NI).Outcomes included preterm birth,macrosomia,and infant mortality.Confounders included demographic characteristics,adequacy of prenatal care,body mass index,smoking,hypertension,and previous preterm birth.Bivariate and multivariate logistic regression assessed differences in outcomes by diabetes status.RESULTS Women with PD-I,PD-NI,and GD-I remained at a significantly increased odds for preterm birth(aOR 2.87,aOR 1.77,and aOR 1.73,respectively)and having a very large baby[macrosomia](aOR 3.01,aOR 2.12,and aOR 1.96,respectively);in reference to non-diabetic women.Women with GDNI were at a significantly increased risk for macrosomia(aOR1.53),decreased risk for their baby to die before their first birthday(aOR 0.41)and no difference in risk for preterm birth in reference to non-diabetic women.CONCLUSION Diabetes is associated with the poor birth outcomes.Clinical management of diabetes during pregnancy and healthy lifestyle behaviors before pregnancy can reduce the risk for diabetes and poor birth outcomes. | Pamela K Xaverius Steven W Howard Deborah Kiel Jerry E Thurman Ethan Wankum Catherine Carter Clairy Fang Romi Carriere | 2022 | World Journal of Clinical Cases2022，10，7： | 2 |
| 7 | Serum proinflammatory cytokines and nutritional status in pediatric chronic liver disease显示文摘AIM: To evaluate the nutritional status and its association with proinflammatory cytokines in children with chronic liver disease.METHODS: We performed a cross-sectional study with 43 children and adolescents, aged 0 to 17 years, diagnosed with chronic liver disease. All patients regularly attended the Pediatric Hepatology Unit and were under nutritional follow up. The exclusion criteria were fever from any etiology at the time of enrollment, inborn errors of the metabolism and any chronic illness. The severity of liver disease was assessed by Child-Pugh, Model for End-stage Liver Disease(MELD) and Pediatric End Stage Liver Disease(PELD) scores. Anthropometric parameters were height/age, body mass index/age and triceps skinfold/age according to World Health Organization standards. The cutoff points for nutritional status were risk of malnutrition(Z-score <-1.00) and malnutrition(Z-score <-2.00). Interleukin-1β(IL-1β), IL-6 and tumor necrosis factor-α levels were assessed by commercial ELISA kits. For multivariate analysis, linear regression was applied to assess the association between cytokine levels, disease severity and nutritional status. RESULTS: The median(25th-75 th centile) age of the study population was 60(17-116)-mo-old, and 53.5% were female. Biliary atresia was the main cause of chronic liver disease(72%). With respect to Child-Pugh score, cirrhotic patients were distributed as follows: 57.1% Child-Pugh A, a mild presentation of the disease, 34.3% Child-Pugh B, a moderate stage of cirrhosis and 8.6% Child-Pugh C, were considered severe cases. PELD and MELD scores were only above the cutoff point in 5 cases. IL-6 values were increased in patients at nutritional risk(34.9%) compared with those who were well-nourished [7.12(0.58-34.23) pg/m L vs 1.63(0.53-3.43) pg/m L; P = 0.02], correlating inversely with triceps skinfold-for-age z-score(rs =-0.61; P < 0.001). IL-6 levels were associated with liver disease severity assessed by Child-Pugh score(P = 0.001). This association remained significant after adjusting for nutritional status in a linear regression model. CONCLUSION: High IL-6 levels were found in children with chronic liver disease at nutritional risk. Inflammatory activity may be related to nutritional status deterioration in these patients. | Daniele Santetti Maria Inês de Albuquerque Wilasco Cristina Toscani Leal Dornelles Isabel Cristina Ribas Werlang Fernanda Urruth Fontella Carlos Oscar Kieling Jorge Luiz dos Santos Sandra Maria Goncalves Vieira Helena Ayako Sueno Goldani | 2015 | World Journal of Gastroenterology2015，21，29： | 2 |
| 8 | Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells 显示文摘 | Park IK Qian D Kiel M | 2003 | Nature2003，423，6937： | 1 |
| 9 | Aberrant methylation of the adenomatous polyposis coli promoter 1A in bronchial aspirates from patients with suspected lung cancer显示文摘 | Grote H J Schmiemann V Kiel S | 2004 | Int J Cancer2004，110，5： | 1 |
| 10 | Measuring the Impact of the Discovery and Cleaning of Identified Hazardous Waste Sites on House Values显示文摘 | Kiel Katherine A | 1995 | Land Economics1995，71，4： | 1 |
| 11 | An in vivo model to study and manipulate the hematopoietic stem cell niche显示文摘 | Song J Kiel MJ Wang Z | 2010 | Blood2010，115，13： | 1 |
| 12 | Snare proteins: One to fuse and three to keep the nascent fusion pore open 显示文摘 | Shi L Shen QT Kiel A | 2012 | Science2012，335，： | 1 |
| 13 | Peroxisome homeostasis in Hansenula polymorpha显示文摘 | Leao AN Kiel JAKW | 2003 | FEMS Yeast Res2003，4，： | 1 |
| 14 | A novel use for trypan blue to minimize epithelial cell proliferation in pediatric cataract surgery显示文摘 | Kiel AW Butler T Gregson R | 2003 | J Pediatr Ophthalmol Strabismus2003，40，2： | 1 |
| 15 | Collaborative practice agreement for diabetes management显示文摘 | KIEL PJ MCCORD AD | 2006 | Am J Health Syst Pharm2006，63，3： | 1 |
| 16 | Multiple Stumbles: A risk factor for falls in community - dwelling olderly 显示文摘 | Teno J Kiel D Mor V | 1990 | J Ame Geriatr Soc1990，38，12： | 1 |
| 17 | Forum on bone and skel- etal muscle interactions: summary of the proceedings of an ASBMR workshop显示文摘 | Bonewald L F Kiel D P Clemens T L | 2013 | J Bone Miner Res2013，28，9： | 1 |
| 18 | Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells显示文摘 | Park IK Qian D Kiel M | 2003 | Nature2003，423，6937： | 1 |
| 19 | Anal gesic effect ofultrasoundguided Wansv dominis plane block after total abdominal hysterectomy: a randomized, double-blind, placebo-controlled trial显示文摘 | Rgjskjer JO Gade E Kiel LB | 2015 | Acta Ob stetri- cia Et Gynecologica Scandinavica2015，94，3： | 1 |
| 20 | Bmi-1 is required formaintenance of adult self-renewing haematopoieticstem cells 显示文摘 | Park IK Qian D Kiel M | 2003 | Nature2003，423，6937： | 1 |
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