|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Golgi protein-73:A biomarker for assessing cirrhosis and prognosis of liver disease patients显示文摘BACKGROUND Reliable biomarkers of cirrhosis,hepatocellular carcinoma(HCC),or progression of chronic liver diseases are missing.In this context,Golgi protein-73(GP73)also called Golgi phosphoprotein-2,was originally defined as a resident Golgi type II transmembrane protein expressed in epithelial cells.As a result,GP73 expression was found primarily in biliary epithelial cells,with only slight detection in hepatocytes.However,in patients with acute or chronic liver diseases and especially in HCC,the expression of GP73 is significantly up-regulated in hepatocytes.So far,few studies have assessed GP73 as a diagnostic or prognostic marker of liver fibrosis and disease progression.AIM To assess serum GP73 efficacy as a diagnostic marker of cirrhosis and/or HCC or as predictor of liver disease progression.METHODS GP73 serum levels were retrospectively determined by a novel GP73 ELISA(QUANTA Lite®GP73,Inova Diagnostics,Inc.,Research Use Only)in a large cohort of 632 consecutive patients with chronic viral and non-viral liver diseases collected from two tertiary Academic centers in Larissa,Greece(n=366)and Debrecen,Hungary(n=266).Aspartate aminotransferase(AST)/Platelets(PLT)ratio index(APRI)was also calculated at the relevant time points in all patients.Two hundred and three patients had chronic hepatitis B,183 chronic hepatitis C,198 alcoholic liver disease,28 autoimmune cholestatic liver diseases,15 autoimmune hepatitis,and 5 with other liver-related disorders.The duration of follow-up was 50(57)mo[median(interquartile range)].The development of cirrhosis,liver decompensation and/or HCC during follow-up were assessed according to internationally accepted guidelines.In particular,the surveillance for the development of HCC was performed regularly with ultrasound imaging and alpha-fetoprotein(AFP)determination every 6 mo in cirrhotic and every 12 mo in non-cirrhotic patients.RESULTS Increased serum levels of GP73(>20 units)were detected at initial evaluation in 277 out of 632 patients(43.8%).GP73-seropositivity correlated at baseline with the presence of cirrhosis(96.4%vs 51.5%,P<0.001),decompensation of cirrhosis(60.3%vs 35.5%,P<0.001),presence of HCC(18.4%vs 7.9%,P<0.001)and advanced HCC stage(52.9%vs 14.8%,P=0.002).GP73 had higher diagnostic accuracy for the presence of cirrhosis compared to APRI score[Area under the curve(AUC)(95%CI):0.909(0.885-0.934)vs 0.849(0.813-0.886),P=0.003].Combination of GP73 with APRI improved further the accuracy(AUC:0.925)compared to GP73(AUC:0.909,P=0.005)or APRI alone(AUC:0.849,P<0.001).GP73 levels were significantly higher in HCC patients compared to non-HCC[22.5(29.2)vs 16(20.3)units,P<0.001)and positively associated with BCLC stage[stage 0:13.9(10.8);stage A:17.1(16.8);stage B:19.6(22.3);stage C:32.2(30.8);stage D:45.3(86.6)units,P<0.001]and tumor dimensions[very early:13.9(10.8);intermediate:19.6(18.4);advanced:29.1(33.6)units,P=0.004].However,the discriminative ability for HCC diagnosis was relatively low[AUC(95%CI):0.623(0.570-0.675)].Kaplan-Meier analysis showed that the detection of GP73 in patients with compensated cirrhosis at baseline,was prognostic of higher rates of decompensation(P=0.036),HCC development(P=0.08),and liver-related deaths(P<0.001)during follow-up.CONCLUSION GP73 alone appears efficient for detecting cirrhosis and superior to APRI determination.In combination with APRI,its diagnostic performance can be further improved.Most importantly,the simple GP73 measurement proved promising for predicting a worse outcome of patients with both viral and nonviral chronic liver diseases. | Nikolaos K Gatselis Tamás Tornai Zakera Shums Kalliopi Zachou Asterios Saitis Stella Gabeta Roger Albesa Gary L Norman Mária Papp George N Dalekos | 2020 | World Journal of Gastroenterology2020，26，34： | 21 |
| 2 | Multiple haploids, triploids, and tetraploids found in modern-day “living fossil” Ginkgo biloba显示文摘Ginkgo biloba,the last extant representative of a lineage of Mesozoic gymnosperms,is one of the few seed plants with an exceptionally long(~300 Myr)evolutionary history free of genome-wide duplications(polyploidy).Despite this genome conservatism,we have recently found a viable spontaneous tetraploid Ginkgo sapling during routine screening of several plants,demonstrating that natural polyploidy is possible in Ginkgo.Here we provide a much wider flow cytometry survey of ploidy in some European Ginkgo collections,and own seedlings(>2200 individuals and~200 cultivars).We found a surprisingly high level of ploidy variation in modern-day Ginkgo and documented altogether 13 haploid,3 triploid,and 10 tetraploid Ginkgo plants or cultivars,most of them being morphologically distinct from common diploids.Haploids frequently produced polyploid(dihaploid)buds or branches.Tetraploids showed some genome size variation.The surveyed plants provide a unique resource for future Ginkgo research and breeding,and they might be used to accelerate the modern diversification of this nearly extinct plant lineage. | PetrŠmarda Lucie Horová Ondřej Knápek Heidi Dieck Martin Dieck Katarína Ražná Pavel Hrubík Laszlo Orlóci Laszlo Papp Kristýna Veselá Pavel Veselý Petr Bureš | 2018 | Horticulture Research2018，5，1： | 4 |
| 3 | Antioxidant potential,tannin and polyphenol contents of seed and pericarp of three Coffea species显示文摘Objective:To investigate the antioxidant activity,total phenolic and total tannin content of the pericarp and the seed of Coffea benghalensis(C.benghalensis) and Coffea liberica compared to Coffea arabica(C.arabica).Methods:The antioxidant potential,total tannin and polyphenol contents of the immature and mature seed and pericarp of C.benghalensis and Coffea liberica were quantified and compared to C.arabica.Enhanced chemiluminescence(ECL).2,2-diphenyl-1-picrylhydruzyl(DPPH).oxygen radical absorbance capacity,Folin-Ciocaltcau method and total tannin content assays were used.Results:Trolox equivalent(TE/g plant material) values obtained by ECL and DPPH methods showed loose correlation(r^2=0.587)while those measured by oxygen radical absorbance capacity assay were higher without correlation in each plant.A closer correlation was detected between the ECL method and the percentage antioxidant activity of the DPPH technique(r^2=0.610 7) in each species,however the immature pericarp of C.benghalensis showed much higher DPPH scavenging potential than was seen in the ECL assay.The immature pericarp of C.benghalensis expressed the highest tannin and polyphenol content,and a high polyphenol level was also detected in the immature seed of C.arabica.The immature pericarp of Bengal and Liberian coffees showed the largest amount of phenolic contents.Conclusions:The obtained data highlight the potential role of C.benghalensis as a new source of natural antioxidants and polyphenols compared to C.arabica. | va Brigitta Patay Nikolett Sali Tams Kszegi Rita Csepregi Viktória Lilla Blaz Tibor Sebastian Németh Tibor Németh Nóra Papp | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，4： | 3 |
| 4 | Relationship between diabetes mellitus and cataract in Hungary显示文摘AIM:To examine the coexistence of diabetes mellitus(DM)and cataract in Hungary.The effects of DM on the cataract surgical results were also in the focus of analysis.METHODS:Statistical data analysis of the results of the Rapid Assessment of Avoidable Blindness with Diabetic Retinopathy(RAAB+DR)module conducted in Hungary in 2015.This cross-sectional,population-based,national survey included 3523 people aged 50 years and over.Participants of the survey were examined on-site.Visual acuity,main cause for visual impairment(using direct and indirect ophthalmoscopes),in case of best corrected visual acuity(BCVA)≤0.5 and blood glucose level(random test with glucometer)were examined.RESULTS:The prevalence of cataract was 23.4%,and DM was 20.0%.The occurrence of cataract steadily increased with age.Among the examined participants with DM,the prevalence of cataract was significantly(P=0.012)higher(+35%)than that in non-diabetic subjects(29.5%vs 21.8%).Following aging(OR=15.2%,P<0.001),DM proved to be the most independent influencing risk factor(OR=49.9%,P<0.001).The presence of DM was neither an influencing factor for complications of cataract surgery,nor for postoperative visual acuity.CONCLUSION:DM appears to be one of the main risk factors for developing cataract.Other risk factors,such as age,sex and environment also play an influencing role.Diabetes does not seem to affect the occurrence of cataract surgical complications. | Anita Pék Dorottya Szabó Gábor LászlóSándor Gábor Tóth András Papp Zoltán Zsolt Nagy Hans Limburg János Németh | 2020 | International Journal of Ophthalmology(English edition)2020，13，5： | 2 |
| 5 | Determination of aliatoxins in food by overpressured-layer chromatographic显示文摘 | Otta K H Papp E Bagocsi B | 2000 | Journal of Chromatography A2000，882，： | 1 |
| 6 | Efficacy and safety of tofacitinib,an oral Janus kinase inhibitor,in the treatment of psoriasis:a Phase 2b randomized placebo-controlled dose-ranging study显示文摘 | Papp K Menter A Strober B | 2012 | Br J Dermatol2012，167，3： | 1 |
| 7 | Pharmacological mecha-nisms contributing to the clinical efficacy of Levosimendan 显示文摘 | Papp Z Csapo K Pollesello P | 2005 | Cardiovasc Drug Rev2005，23，1： | 1 |
| 8 | Oral apremilast in the treatment of active psoriatic arthritis: results of a multicenter, randomized, double-blind, placebo-controlled study 显示文摘 | Schett G Wollenhaupt J Papp K | 2012 | Arthritis Rheum2012，64，10： | 1 |
| 9 | Intraarticular pilon fracture of the tibia显示文摘 | Heifer DL Koval K Papps J | 1994 | Clin Orthop1994，298，： | 1 |
| 10 | Determination of aflatoxins in food by overpressured-layer Chromatography显示文摘 | Otta K H Papp E Bagoesi B | 2000 | Journal of Chromatography A2000，882，： | 1 |
| 11 | The treatment of moderate to severe psori asis with a new anti-CD11a monoclonalantibody显示文摘 | Papp K Bissonnette R Krueger JG | | 0，，： | 1 |
| 12 | Interactions between orexin-immunoreactive fibers and adrenaline or rons of the lower brainstem in rats and mice 显示文摘 | Puskes N Papp RS Gallatz K | 2010 | Peptides2010，31，： | 1 |
| 13 | Efficacy of apremilast in the treatment of moderate to severe psoriasis: a randomised controlled trial 显示文摘 | Papp K Cather J C Rosoph L | 2012 | Lancet2012，380，9843： | 1 |
| 14 | Aspirin Resistance 显示文摘 | Feher G Kohai K Papp E | 2006 | DrugsAging2006，23，7： | 1 |
| 15 | Impact of metabolic genotypes on levels of biomarkers of genotoxic exposure 显示文摘 | Schoket B Papp G Levay K | 2001 | Murat Res2001，482，： | 1 |
| 16 | Microchemical Journal显示文摘 | Papp E H-Otta K Zaray G Mincsovics E | 2002 | 73 : 392002，73，： | 1 |
| 17 | Pharmacological mechanisms contributing to the clinical efficacy of levosimendan显示文摘 | Papp Z Csap6 K Pollesello P | 2005 | Cardio- vasc Drug Rev2005，23，1： | 1 |
| 18 | Infliximab induction and maintenance therapy for moderate-to-severe psoriasis: a phase Ill, multicentre, double-blind trial显示文摘 | Reich K Nestle FO Papp K | 2005 | Lancet2005，366，9494： | 1 |
| 19 | The progression of burn depth in experimental burns:a histological and methodological study显示文摘 | Papp A Kiraly K Harma M | 2004 | Burns2004，30，7： | 1 |
| 20 | Surface acidity and photocatalytic activeity of TiO2, WO3/TiO2 and MoO3/TiO2 photocatalysts显示文摘 | PAPP J SOLED S DWIGHT K | 1994 | Chem Mater1994，，6： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员