|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Synovial sarcoma:a rare presentation of parapharyngeal mass显示文摘Synovial sarcoma is a rare soft tissue sarcoma of the head and neck region involving the parapharyngeal space.The diagnosis of synovial sarcoma can be very challenging to the pathologists.We present a rare case of parapharyngeal synovial sarcoma in a young female patient who had a two-month history of left cervical intumescent mass at level II.The fine needle aspiration cytology of the mass was proved inconclusive.Transcervical excision of the mass was performed and the first case of parapharyngeal sarcoma was identified in our center by fluorescence in situ hybridization(FISH) technique.Repeat imaging revealed residual tumor.The patient successfully underwent a second excision of the residual tumor and received adjuvant radiotherapy. | Mohd Mokhtar Shaariyah Ami Mazita Mansor Masaany Mohd Yunus Razif Mohamed Rose Isa Abdullah Asma1 | 2010 | Chinese Journal of Cancer2010，29，6： | 7 |
| 2 | Eurycoma Longifolia as a potential adoptogen of male sexual health: a systematic review on clinical studies显示文摘Eurycoma longifolia(EL) has been well recognized as a booster of male sexual health. Over the past few decades, numerous in vivo animal studies and human clinical trials have been conducted across the globe to explore the promising role of EL in managing various male sexual disorders, which include erectile dysfunction, male infertility, low libido, and downregulated testosterone levels. The aim of the present review is to analyze and summarize the literature on human clinical trials which revealed the clinical significance and therapeutic feasibility of EL in improving male sexual health. This systematic review is focused on the following databases: Medline, Wiley Online Library, Bio Med Central, Hindawi, Web of Knowledge, Pub Med Central and Google Scholar, using search terms such as 'Eurycoma longifolia', 'EL', 'Tongkat Ali', 'male sexual health', 'sexual infertility', 'erectile dysfunction', 'male libido', and 'testosterone levels'. Notably, only human clinical studies published between 2000 and 2014 were selected and thoroughly reviewed for relevant citations. Out of 150 articles, 11 met the inclusion criteria. The majority of articles included were randomized placebo-controlled trials, multiple cohort studies, or pilot trials. All these studies demonstrated considerable effects of EL on male sexual health disorders. Among them, 7 studies revealed remarkable association between the use of EL and the efficacy in the treatment of male sexual disorders, and remaining 4 studies failed to demonstrate sufficient effects on male sexual health. In summary, there is convincing evidence for the prominence of EL in improving the male sexual health. The review also substantiates the use of current methodology in the development of novel and more rationale natural herbal medicines for the management of male sexual disorders. | Hnin Ei Thu Isa Naina Mohamed Zahid Hussain Putri Ayu Jayusman Ahmad Nazrun Shuid | 2017 | Chinese Journal of Natural Medicines2017，15，1： | 7 |
| 3 | Ocular syphilis: resurgence of an old disease in modern Malaysian society显示文摘Dear Editor,Syphilis is a spirochaetal bacterial sexually transmitted disease(STD) caused by Treponema pallidum. Outbreaks of syphilis have been described in Western communities, especially in men who have sex with men(MSM) and people infected with human immunodeficiency virus(HIV)^([1]). Reports of the condition from Asian countries remains limited, this | Mushawiahti Mustapha Zakaria Abdollah Amin Ahem Hazlita Mohd Isa Mae-Lynn Catherine Bastion Norshamsiah Md Din | 2018 | International Journal of Ophthalmology(English edition)2018，11，9： | 4 |
| 4 | Catalytic steam reforming of tar for enhancing hydrogen production from biomass gasification:a review显示文摘Presently,the global search for alternative renewable energy sources is rising due to the depletion of fossil fuel and rising greenhouse gas(GHG)emissions.Among alternatives,hydrogen(H2)produced from biomass gasification is considered a green energy sector,due to its environmentally friendly,sustainable,and renewable characteristics.However,tar formation along with syngas is a severe impediment to biomass conversion efficiency,which results in process-related problems.Typically,tar consists of various hydrocarbons(HCs),which are also sources for syngas.Hence,catalytic steam reforming is an effective technique to address tar formation and improve H2 production from biomass gasification.Of the various classes in existence,supported metal catalysts are considered the most promising.This paper focuses on the current researching status,prospects,and challenges of steam reforming of gasified biomass tar.Besides,it includes recent developments in tar compositional analysis,supported metal catalysts,along with the reactions and process conditions for catalytic steam reforming.Moreover,it discusses alternatives such as dry and autothermal reforming of tar. | Ru Shien TAN Tuan Amran TUAN ABDULLAH Anwar JOHARI Khairuddin MD ISA | 2020 | Frontiers in Energy2020，14，3： | 4 |
| 5 | Evaluation of early results of quick-chop phacoemulsification in the patients with high myopic cataract显示文摘AIM:To assess the early surgical outcomes of quickchop phacoemulsification technique in patients with high myopia.METHODS:The data of patients with high myopia who underwent quick-chop phacoemulsification were reviewed retrospectively. There were 42 eyes of 31 patients. The axial length was more than 26 mm in all eyes. All eyes underwent quick-chop phacoemulsification surgery with the placement of an intraocular lens(IOL) in the capsular bag. Postoperative visits were performed at 1, 3d; 2wk,1mo. Early postoperative best corrected visual acuity(BCVA), preoperative and postoperative corneal endothelial cell density(ECD), central corneal thickness(CCT) and postoperative complications were assessed.Paired sample t-test or Wilcoxon tests were used to compare data between preoperative and postoperative data.· RESULTS:There was no statistically significant difference between preoperative and postoperative ECD and CCT. Retinal detachment was developed in one eye at postoperative first day. There was an iris prolapsus from side port insicion.· CONCLUSION:Quick-chop phacoemulsification technique is a safe surgical technique. However we can encounter some complications in high myopic eyes due to histopathological differences. Both side port and clear corneal tunnel insicion size is crucial for preventing postoperative complications. If any persistent leakage is noticed, suture should be placed. | Ali Akal Tugba Goncu Sevin Soker Cakmak Isa Yuvaci Mustafa Atas Süleyman Demircan Omer Faruk Yilmaz | 2014 | International Journal of Ophthalmology(English edition)2014，7，5： | 4 |
| 6 | Biomass and dominance of conservative species drive above-ground biomass productivity in a mediterranean-type forest of Chile显示文摘Background: Forest productivity has a pivotal role in human well-being. Vegetation quantity, niche complementarity, mass-ratio, and soil resources are alternative/complementary ecological mechanisms driving productivity. One challenge in current forest management depends on identifying and manipulating these mechanisms to enhance productivity. This study assessed the extent to which these mechanisms control aboveground biomass productivity(AGBP) of a Chilean mediterranean-type matorral. AGBP measured as tree aboveground biomass changes over a 7-years period, was estimated for twelve 25 m × 25 m plots across a wide range of matorral compositions and structures. Variables related to canopy structure, species and functional diversity, species and functional dominance, soil texture, soil water and soil nitrogen content were measured as surrogates of the four mechanisms proposed. Linear regression models were used to test the hypotheses. A multimodel inference based on the Akaike’s information criterion was used to select the best models explaining AGBP and for identifying the relative importance of each mechanism.Results: Vegetation quantity(tree density) and mass-ratio(relative biomass of Cryptocarya alba, a conservative species) were the strongest drivers increasing AGBP, while niche complementarity(richness species) and soil resources(sand, %) had a smaller effect either decreasing or increasing AGBP, respectively. This study provides the first assessment of alternative mechanisms driving AGBP in mediterranean forests of Chile. There is strong evidence suggesting that the vegetation quantity and mass-ratio mechanisms are key drivers of AGBP, such as in other tropical and temperate forests. However, in contrast with other studies from mediterranean-type forests, our results show a negative effect of species diversity and a small effect of soil resources on AGBP.Conclusion: AGBP in the Chilean matorral depends mainly on the vegetation quantity and mass-ratio mechanisms.The findings of this study have implications for matorral restoration and management for the production of timber and non-timber products and carbon sequestration. | Ariel Isaías Ayma-Romay Horacio E.Bown | 2019 | Forest Ecosystems2019，6，4： | 4 |
| 7 | Digital Soil Mapping Using Artificial Neural Networks and Terrain-Related Attributes显示文摘Detailed soil surveys involve costly and time-consuming work and require expert knowledge. Since soil surveys provide information to meet a wide range of needs, new methods are necessary to map soils quickly and accurately. In this study, multilayer perceptron artificial neural networks(ANNs) were developed to map soil units using digital elevation model(DEM) attributes. Several optimal ANNs were produced based on a number of input data and hidden units. The approach used test and validation areas to calculate the accuracy of interpolated and extrapolated data. The results showed that the system and level of soil classification employed had a direct effect on the accuracy of the results. At the lowest level, smaller errors were observed with the World Reference Base(WRB)classification criteria than the Soil Taxonomy(ST) system, but more soil classes could be predicted when using ST(7 soils in the case of ST vs. 5 with WRB). Training errors were below 11% for all the ANN models applied, while the test error(interpolation error) and validation error(extrapolation error) were as high as 50% and 70%, respectively. As expected, soil prediction using a higher level of classification presented a better overall level of accuracy. To obtain better predictions, in addition to DEM attributes, data related to landforms and/or lithology as soil-forming factors, should be used as ANN input data. | Mohsen BAGHERI BODAGHABADI José Antonio MARTINEZ-CASASNOVAS Mohammad Hasan SALEHI Jahangard MOHAMMADI Isa ESFANDIARPOOR BORUJENI Norair TOOMANIAN Amir GANDOMKAR | 2015 | Pedosphere2015，25，4： | 3 |
| 8 | Monochromatic green light stimulation during incubation shortened the hatching time via pineal function in White Leghorn eggs显示文摘Background:Effect of monochromatic green light illumination on embryo development has been reported in chickens.The avian pineal gland is an important photo-endocrine organ formed by a mediodorsal protrusion during embryonic development.However,the involvement of pineal gland in the light transduction process remains to be elucidated.In the present study,we investigated the influence of monochromatic green light on hatching time and explored the possible mechanism via pineal function.Results:A total of 600 eggs of White Leghorn(Shaver strain)were incubated under photoperiods of either 12 h of light and 12 h of darkness using monochromatic green light(12L:12D group)or 24 h of darkness(0L:24D group)for 18 d.Compared to 0L:24D group,the green light stimulation shortened the hatching time without extending the hatch window or impairing hatchability.The liver of embryos incubated in the 12L:12D light condition was heavier than those of the 0L:24D group on d 21 post incubation which may be linked to the observed increase in the serum concentration of insulin-like growth factor 1(IGF-1);primary secretion of the liver.Histological structure analysis of pineal gland demonstrated that the light stimulation increased follicle area,wall thickness and lumen area on d 10 and d 12 post incubation.Rhythmic function analysis demonstrated that three clock related genes(brain and muscle ARNT-like-1,BMAL1;circadian locomotor output cycles kaput,CLOCK;and cryptochrome-1,CRY1)and a melatonin rate-limiting enzyme related gene(arylalkylamine N-acetyltransferase,AANAT)were rhythmically expressed in the pineal gland of the 12L:12D group,but not in the 0L:24D group.Simultaneously,the light stimulation also increased the concentration of melatonin(MT),which was linked to hepatocyte proliferation and IGF-1 secretion in previous studies.Conclusions:The 12L:12D monochromatic green light stimulation during incubation shortened hatching time without impairing hatching performance.Pineal gland’s early histological development and maturation of its rhythmic function were accelerated by the light stimulation.It may be the key organ in the photo-endocrine axis that regulates embryo development,and the potential mechanism could be through enhanced secretion of MT in the 12L:12D group which promotes the secretion of IGF-1. | Panlin Wang Yanyan Sun Yunlei Li Jing Fan Yunhe Zong Adamu Mani Isa Lei Shi Yuanmei Wang Aixin Ni Pingzhuang Ge Linlin Jiang Shixiong Bian Hui Ma Zhengdong Yuan Xiaolin Liu Jilan Chen | 2021 | Journal of Animal Science and Biotechnology2021，12，3： | 3 |
| 9 | Exploring dynamic biomedical algorithm of Eurycoma longifolia Jack and its bioactive phytochemicals: A review of pharmacokinetic and pharmacodynamic implications and future prospects显示文摘Eurycoma longifolia Jack(E.longifolia) is a well-recognized traditional herbal medicine that offers a wide dynamic range of biomedical applications including anti-osteoporotic, anticancer, anti-proliferative, anti-malarial, antimicrobial, antioxidant, aphrodisiac, antiinflammatory, anxiolytic, anti-diabetic, anti-rheumatism and anti-ulcer properties.This review aims to overview the pharmacokinetic and a pharmacodynamic algorithm of E.longifolia and its bioactive components.Analysis of pharmacokinetic profile revealed that E.longifolia exhibit higher bioavailability, high volume of distribution, slow elimination rate, and does not show inhibitory effects on cytochrome P450 isoenzymes.E.longifolia has been used, alone or in combination with other pharmacological agents, in the form of crude extracts, standard extracts, or decoctions of different plant parts(i.e., herbs, shrubs, stem, leaves, and roots) for the treatment of various ailments in animals and humans.Among various bioactive constituents, eurycomanone has been found to be the most remarkable, super-stable, versatile, and most potent phytochemical(isolated or extracted from root extracts) against various types of animals and human diseases.Based on its well-established pharmacokinetic and pharmacodynamic profiles, we suggested that E.longifolia can be a well-accepted complementary and alternative medicine for the treatment of different types of human ailments. | Hnin Ei Thu Zahid Hussain Isa Naina Mohamed Ahmad Nazrun Shuid | 2018 | Asian Pacific Journal of Tropical Medicine2018，11，2： | 2 |
| 10 | Combination of sensitivity and uncertainty analyses for sediment transport modeling in sewer pipes显示文摘Mitigation of sediment deposition in lined open channels is an essential issue in hydraulic engineering practice.Hence,the limiting velocity should be determined to keep the channel bottom clean from sediment deposits.Recently,sediment transport modeling using various artificial intelligence(AI)techniques has attracted the interest of many researchers.The current integrated study highlights unique insight for modeling of sediment transport in sewer and urban drainage systems.A novel methodology based on the combination of sensitivity and uncertainty analyses with a machine learning technique is proposed as a tool for selection of the best input combination for modeling process at non-deposition conditions of sediment transport.Utilizing one to seven dimensionless parameters,127 models are developed in the current study.In order to evaluate the different parameter combinations and select the training and testing data,four strategies are considered.Considering the densimetric Froude number(Fr)as the dependent parameter,a model with independent parameters of volumetric sediment concentration(CV)and relative particle size(d/R)gave the best results with a mean absolute relative error(MARE)of 0.1 and a root means square error(RMSE)of 0.67.Uncertainty analysis is applied with a machine learning technique to investigate the credibility of the proposed methods.The percentage of the observed sample data bracketed by 95%predicted uncertainty bound(95PPU)is computed to assess the uncertainty of the best models. | Isa Ebtehaj Hossein Bonakdari Mir Jafar Sadegh Safari Bahram Gharabaghi Amir Hossein Zaji Hossien Riahi Madavar Zohreh Sheikh Khozani Mohammad Sadegh Es-haghi Aydin Shishegaran Ali Danandeh Mehr | 2020 | International Journal of Sediment Research2020，35，2： | 2 |
| 11 | An experimental and numerical study of chemically enhanced water alternating gas injection显示文摘In this work, an experimental study combined with numerical simulation was conducted to investigate the potential of chemically enhanced water alternating gas(CWAG) injection as a new enhanced oil recovery method.The unique feature of this new method is that it uses alkaline, surfactant, and polymer additives as a chemical slug which is injected during the water alternating gas(WAG) process to reduce the interfacial tension(IFT) and simultaneously improve the mobility ratio. In essence, the proposed CWAG process involves a combination of chemical flooding and immiscible carbon dioxide(CO2)injection and helps in IFT reduction, water blocking reduction, mobility control, oil swelling, and oil viscosity reduction due to CO2 dissolution. Its performance was compared with the conventional immiscible water alternating gas(I-WAG) flooding. Oil recovery utilizing CWAG was better by 26 % of the remaining oil in place after waterflooding compared to the recovery using WAG conducted under similar conditions. The coreflood data(cumulative oil and water production) were history matched via a commercial simulator by adjusting the relative permeability curves and assigning the values of the rock and fluid properties such as porosity, permeability, and the experimentally determined IFT data. History matching of the coreflood model helped us optimize the experiments and was useful in determining the importance of the parameters influencing sweep efficiency in the CWAG process. The effectiveness of the CWAG process in providing enhancement of displacement efficiency is evident in the oil recovery and pressure response observed in the coreflood. The results of sensitivity analysis on CWAG slug patterns show that the alkaline–surfactant–polymer injection is more beneficial after CO2 slug injection due to oil swelling and viscosity reduction. The CO2 slug size analysis shows that there is an optimum CO2 slug size,around 25 % pore volume which leads to a maximum oil recovery in the CWAG process. This study shows that the ultralow IFT system, i.e., IFT equaling 10-2or 10-3m N/m, is a very important parameter in CWAG process since the water blocking effect can be minimized. | Saeed Majidaie Mustafa Onur Isa M.Tan | 2015 | Petroleum Science2015，12，3： | 2 |
| 12 | 二硫戊环衍生物和类似物的合成及其iNOS/PAF双重抑制剂活性(英文)显示文摘目的 :研究双重作用的新型抗炎药物。方法 :合成二硫戊环衍生物和类似物 ,研究这些化合物及选择性的PAF受体拮抗剂L6 5 9,989对PAF受体的结合 ,和对IFN γ或LPS诱导的NO生成 ,以及对iNOSmRNA表达的影响。结果 :合成了 4 0个新化合物 ,其中 19个在不同程度上抑制NO生成。选择 3个活性最高的化合物 (2 ,19,2 0 )进行深入研究 ,发现它们能抑制iNOSmRNA的表达 ,其抑制NO生成的效力从高到低依次为 :19,2 0 ,2 ,L6 5 9,989,其拮抗PAF受体的活性从高到低依次为 :L6 5 9,989,2 0 ,2 ,19。L6 5 9,989对NO生成和iNOSmRNA表达无影响。受试物的PAF受体拮抗活性与iNOS抑制活性之间无简单关联。结论 :二硫戊环类化合物是iNOS表达的新型调控剂 ,其中一些具有抑制iNOS的诱导和拮抗PAF受体的双重活性。 | 张奕华 HUSSAINI Isa M SHEN Tsung Y | 2003 | 中国药科大学学报2003，34，3： | 2 |
| 13 | Pentacam Scheimpflug成像测量晶状体密度与torsional超声乳化参数间的关系(英文)显示文摘目的:评价运用Pentacam Scheimpflug成像测量晶状体核密度与torsinal超声乳化动力学参数(如:运用于老年核性白内障患者的超声能量水平、液体留存时间及液体量)间的关系。方法:此研究为前瞻性双盲研究。瞳孔扩大后运用Pentacam Scheimpflug 成像进行测量。白内障的等级由pentacam核密度测定法[ pentacam nucleus densitometry, PND;又名Pentacam核分段( Pentacam nucleus staging, PNS)软件]自主分为1到5个等级。超声乳化术后,自动在Infiniti Ozil IP超声乳化系统监视器上计算并显示总超声( U/S)时间、累计耗散能、Torsional U/S时间以及估算液体的使用情况。组间差异运用单因素方差分析( ANOVA )进行评定,多组分析运用Tamhane测试法,PND测量的晶状体密度与torsinal超声乳化动力学参数间的关系运用Spearman相关分析进行评定。界定P〈0.05时具有统计学意义。结果:此项研究包括125例患者(125眼),平均年龄为69.7±9.4岁(范围:48~88岁),其中61例男性、64例女性。 U/S总时间、torsional U/S时间、CDE及估算液体使用情况的范围分别为0.70~158.9s,0.70~158.5s,0.11~42.65和21~98mL. PND组间数据有统计学差异。评估超声乳化动力学参数与PND数据间关系后得出PND数据与总超声时间( r=0.767; P〈0.01)、torsional超声时间(r=0.767;P〈0.01)、CDE(r=0.758; P〈0.01)及液体使用量(r=0.602;P〈0.01)均有显著相关性。结论:白内障手术前运用PND评分法获得核密度的客观程度可为超声乳化术中的参数提供可靠的预见性。因此,对每一位患者进行每一单项超声乳化参数的评估,有助于降低超声乳化能量的使用量,减少内皮细胞丢失和类固醇的使用,加速视力的恢复。 | Suleyman Demircan Mustafa Atas Murat Koksal Emine Pangal Isa Yuvaci Altan Goktas | 2014 | 国际眼科杂志2014，14，10： | 2 |
| 14 | Trend and magnitude of changes in climate variables and reference evapotranspiration over 116-yr period in the Platte River Basin, central Nebraska–USA显示文摘 | Suat Irmak Isa Kabenge Kari E. Skaggs Denis Mutiibwa | 2011 | Journal of Hydrology2011，，： | 2 |
| 15 | An analysis of shear stress distribution in circular channels with sediment deposition based on Gene Expression Programming显示文摘Knowledge of the boundary shear stress distribution in channels is important because it is a key factor affecting on erosion and sedimentation rates. The presence of sediment deposits in sewers is often reported during operation, and circular channels are frequently used in sewer networks. Gene expression programming(GEP) is applied in this study to determine an equation for evaluating the shear stress distribution along the wetted perimeter of a circular channel with a flat bed, because of the presence of sediment on the bed. In view of the parameters affecting the shear stress distribution, five dimensionless parameters are applied to develop six GEP models to be used with 905 experimental data. The impact of the shear stress parameters is studied using the six GEP models and by dividing the wetted perimeter into wall and bed sections. Two equations are extracted from the GEP models' output to estimate wall and bed shear stresses. The best model results are compared with a well-known equation based on the entropy concept. The GEP model predictions of wall and bed shear stresses are very similar to the experimental outcomes, whereas the entropy-based model overestimates the shear stress distribution.The proposed GEP models demonstrate superior performance in estimating the shear stress distribution with a mean absolute percentage error(MAPE) of 3.79% compared to an existing equation with MAPE of 9.52%. | Zohreh Sheikh Khozani Hossein Bonakdari Isa Ebtehaj | 2017 | International Journal of Sediment Research2017，32，4： | 2 |
| 16 | 基于自清洁理念的下水道中沉积物运移的设计标准(英文)显示文摘研究目的:通过比较沉积物运移的不同标准,经选优得出更高精度的标准。创新要点:使用更少的参数和分情况来获得高精度的设计标准。研究方法:1.按状态将下水道中沉积物分成两类:没有沉淀的沉积物和沉淀的推移质;2.利用已有设计标准的方程,运用非线性回归和MINITAB软件,得到不同情况下的最佳方程式;3.用实验所得的值对各方程预测值进行验证,比较各方程式的预测精度。重要结论:1.本文提出的方程式可以预测不同情况下的沉积物运移;2.相比于其它标准,本文提出的方程式形式简单、所需参数少和参数估计简单,并且预测精度更高。 | Isa EBTEHAJ Hossein BONAKDARI Ali SHARIFI | 2014 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2014，15，11： | 2 |
| 17 | Postoperative fluid management显示文摘Postoperative care units are run by an anesthesiologist or a surgeon, or a team formed of both. Management of postoperative fluid therapy should be done considering both patients' status and intraoperative events. Types of the fluids, amount of the fluid given and timing of the administration are the main topics that determine the fluid management strategy. The main goal of fluid resuscitation is to provide adequate tissue perfusion without harming the patient. The endothelial glycocalyx dysfunction and fluid shift to extracellular compartment should be considered wisely. Fluid management must be done based on patient's body fluid status. Patients who are responsive to fluids can benefit from fluid resuscitation, whereas patients who are not fluid responsive are more likely to suffer complications of overhydration. Therefore, common use of central venous pressure measurement, which is proved to be inefficient to predict fluid responsiveness, should be avoided. Goal directed strategy is the most rational approach to assess the patient and maintain optimum fluid balance. However, accessible and applicable monitoring tools for determining patient's actual fluid need should be further studied and universalized. The debate around colloids and crystalloids should also be considered with goal directed therapies. Advantages and disadvantages of each solution must be evaluated with the patient's specific condition. | Selami Ilgaz Kayilioglu Tolga Dinc Isa Sozen Akin Bostanoglu Mukerrem Cete Faruk Coskun | 2015 | World Journal of Critical Care Medicine2015，4，3： | 2 |
| 18 | Estimation of the Maximum Annual Loads Modeling for Kingdom of Bahrain显示文摘 | Isa Salman Qamber | 2013 | Journal of Energy and Power Engineering2013，7，10： | 2 |
| 19 | 鸽毛滴虫外泌体的分离鉴定及蛋白质谱分析显示文摘旨在研究鸽毛滴虫是否分泌外泌体及虫源外泌体的蛋白组成和功能,为探索外泌体在鸽毛滴虫寄生过程中的作用奠定基础。采集鸽毛滴虫感染个体口腔及咽部病灶处样本,进行分离培养及纯化,使用显微镜鉴定虫体形态,提取DNA并比对其序列,确定培养物是否为鸽毛滴虫虫株;通过对培养液上清进行超速离心,透射电子显微镜、Western blot技术和纳米颗粒追踪分析(nanoparticle tracking analysis,NTA)鉴定培养液上清中是否含有虫源外泌体;最后通过Label-free技术分析虫源外泌体的蛋白质谱表达。结果显示,虫体具有明显的毛滴虫形态及特征,与鸽毛滴虫ITS1/5.8S/ITS2基因比对率达98%。从虫体培养液中提取的囊泡经透射电子显微镜观察具有明显的茶托样结构;NTA结果显示,粒径集中于125.1 nm,峰值粒径为132.3 nm,占比99.3%;CD63和TSG101等外泌体标记蛋白的条带明显,表明提取物为外泌体。质谱结果显示,烯醇酶、3-磷酸甘油醛-脱氢酶、磷酸甘油酸变位酶和延伸因子为表达量较高的蛋白质。GO功能注释显示外泌体蛋白富集到细胞内和胞浆等细胞组分,发挥GTP连接和GTP酶活性功能,参与小GTPase介导的信号转导与糖酵解等过程。KEGG通路富集表明,虫源外泌体蛋白主要富集于代谢途径、糖酵解/糖异生、抗生素的生物合成和次级代谢产物的生物合成等通路。本研究发现鸽毛滴虫可以分泌外泌体,并对虫源外泌体进行蛋白质谱分析,提示虫源外泌体中的蛋白质在寄生虫的能量代谢、信号转导及生物合成等过程中发挥重要作用。 | 倪爱心 李云雷 葛平壮 王攀林 Adamu Mani Isa 石雷 范静 孙研研 孙鸿 麻慧 陈继兰 | 2020 | 畜牧兽医学报2020，51，7： | 2 |
| 20 | Hypoxia in head and neck cancer 显示文摘 | Isa AY Ward TH West CM | 2006 | Br J Radiol2006，79，946： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员