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| 1 | Lactogenic hormones alter cellular and extracellular microRNA expression in bovine mammary epithelial cell culture显示文摘Background: Bovine milk contains not only a variety of nutritional ingredients but also micro RNAs(mi RNAs) that are thought to be secreted by the bovine mammary epithelial cells(BMECs). The objective of this study was to elucidate the production of milk-related mi RNAs in BMECs under the influence of lactogenic hormones.Results: According to a microarray result of milk exosomal mi RNAs prior to cellular analyses, a total of 257 mi RNAs were detected in a Holstein cow milk. Of these, 18 major mi RNAs of interest in the milk were selected for an expression analysis in BMEC culture that was treated with or without dexamethasone, insulin, and prolactin(DIP) to induce a lactogenic differentiation. Quantitative polymerase chain reaction(q PCR) results showed that the expressions of mi R-21–5p(P = 0.005), mi R-26a(P = 0.016), and mi R-320a(P = 0.011) were lower in the DIP-treated cells than in the untreated cells. In contrast, the expression of mi R-339a(P = 0.017) in the cell culture medium were lower in the DIP-treated culture than in the untreated culture. Intriguingly, the mi R-148 a expression in cell culture medium was elevated by DIP treatment of BMEC culture(P = 0.018). The medium-to-cell expression ratios of mi R-103(P = 0.025), mi R-148a(P < 0.001), and mi R-223(P = 0.013) were elevated in the DIP-treated BMECs, suggesting that the lactogenic differentiation-induced secretion of these three mi RNAs in BMECs. A bioinformatic analysis showed that the mi RNAs down-regulated in the BMECs were associated with the suppression of genes related to transcriptional regulation, protein phosphorylation, and tube development.Conclusion: The results suggest that the mi RNAs changed by lactogenic hormones are associated with milk protein synthesis, and mammary gland development and maturation. The elevated mi R-148 a level in DIP-treated BMECs may be associated with its increase in milk during the lactation period of cows. | Susumu Muroya Tatsuro Hagi Ataru Kimura Hisashi Aso Masatoshi Matsuzaki Masaru Nomura | 2016 | Journal of Animal Science and Biotechnology2016，7，4： | 5 |
| 2 | 空心轴未装轴承的角度编码器显示文摘 | Dr.-Ing.Rainer HagI | 2003 | 制造技术与机床2003，，4： | 4 |
| 3 | Identification of volatile compounds isolated from round kumquat (Fortunellajaponica Swingle)显示文摘 | UMANO K HAGI Y TAMURA T | 1994 | Journal of Agricultural and Food Chemistry1994，42，9： | 1 |
| 4 | A targeted review of study outcomes with short ( < or =7m) endosseous dental implants placed in partially edentulous patients 显示文摘 | Hagi D Deporter DA Pilliar RM | 2004 | Periodontology2004，75，6： | 1 |
| 5 | Volatile constituents of green and ripened pineapple显示文摘 | Katsumi Umano Yukio Hagi | 1992 | Journal of agricultural and food chemistry1992，40，4： | 1 |
| 6 | Expression of a human gene for interferon in yeast显示文摘 | HITZEMAN R A HAGIE F E LEVINE | 1981 | Nature1981，293，： | 1 |
| 7 | Evaluation of second and fourth yearundergraduate medical students' perception and acceptance of the problem-bensed learning process显示文摘 | Hagi S K Al-Shawwa L A | 2011 | Saudi Med J2011，32，10： | 1 |
| 8 | Estimation of net physical transport and hydraulic residence times for a coastal plain estuary using box models显示文摘 | James D. Hagy Walter R. Boynton Lawrence P. Sanford | 2000 | Estuaries2000，，3： | 1 |
| 9 | An empirical research study on Japanese sport tourism in sport-for-all events:Case studies of a single-night event and a multiple-night event显示文摘 | Nogawa Haruo Yamaguchi Yasuo Hagi Yumiko | 1996 | Journal of Travel Research1996，35，2： | 1 |
| 10 | Experimental evaluation of beam-bending method of determining glass viscosities in the range 108 to 1015 poises显示文摘 | Hagy H E | 1963 | American Ceramic Society1963，46，： | 1 |
| 11 | Effects of the omega-6: omega-3 fatty acid ratio of fat emulsions on the fatty acid composition in cell membranes and the anti-inflammatory action 显示文摘 | Hagi A Nakayama M Shinzaki W | 2010 | JPEN J Parenter Enteral Nutr2010，34，3： | 1 |
| 12 | Expression of human gene for interferon in yeast显示文摘 | Hitzcmon RA Hagie FE Levine HL | 1981 | Nature1981，293，： | 1 |
| 13 | Biomechanics of walking, running, and sprinting显示文摘 | MANN RA HAGY J | 1980 | Am J Physiol1980，8，： | 1 |
| 14 | The novel compound NO-1886 elevates plasma high-density lipoprotein cholesterol levels in hamsters and rabbits by increasing by lipoprotein lipase without any effect on cholesterol ester transfer protein activity显示文摘 | Tsutsumi K Inoue Y Hagi A | 1997 | Metabolism1997，46，3： | 1 |
| 15 | VzO5-WO3/TiO2- SiO2-SO42- catalysts: influence of active components and supports on activities in the selective catalytic reduction of NO by NH3 and in the oxidation of SO2 显示文摘 | Motonobu Kobayashi Mitsuharu Hagi | 2006 | Applied Catalysis B: Environmental2006，63，12： | 1 |
| 16 | The novel compound NO1886 activates lipoprotein lipase in primary cultured adipose and skeletal muscle cells显示文摘 | HIRAI I KOHRI H | 1997 | Biol Pharm Bull1997，20，： | 1 |
| 17 | Phytoplankton production and nutrient distributions in a subtropical estuary: Importance of freshwater flow 显示文摘 | MURRELL M C HAGY J D LORES E M | 2007 | Estuaries and Coasts2007，30，3： | 1 |
| 18 | Precison thermal expan sion measurements on low expansion optical materi als 显示文摘 | Plummer W A Hagy H E | 1968 | Applied Optics1968，7，5： | 1 |
| 19 | The history and contemporary challenges of the US Food and Drug Administration 显示文摘 | Borchers AT Hagie F Keen CL | 2007 | Clin Ther2007，29，1： | 1 |
| 20 | Effects of the ome- ga-6:omega-3 fatty acid ratio of fat emulsions on the fatty acid composition in cell membranes and the anti-inflammatory ac- tion 显示文摘 | Hagi A Nakayama M Shinzaki W | 2010 | J Parenter Enteral Nutr2010，34，3： | 1 |
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