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| 1 | Detection and characterization of murine colitis and carcinogenesis by molecularly targeted contrast-enhanced ultrasound显示文摘AIM To study mucosal addressin cellular adhesion molecule-1(MAd CAM-1) and vascular endothelial growth factor(VEGF)-targeted contrast enhanced ultrasound(CEUS) for the assessment of murine colitis and carcinogenesis. METHODS C57BL/6 mice were challenged with 3% dextran sodium-sulfate(DSS) for three, six or nine days to study the development of acute colitis. Ultrasound was performed with and without the addition of unspecific contrast agents. MAd CAM-1-targeted contrast agent was used to detect and quantify MAd CAM-1 expression. Inflammatory driven colorectal azoxymethane(AOM)/DSS-induced carcinogenesis was examined on day 42 and 84 using VEGF-targeted contrast agent. Highly specific tissue echogenicity was quantified using specialized software. Sonographic findings were correlated to tissue staining, western blot analysis and immunohistochemistry to quantify the degree of inflammation and stage of carcinogenesis. RESULTS Native ultrasound detected increased general bowel wall thickening that correlated with more progressed and more severe DSS-colitis(healthy mice: 0.3 mm ± 0.03 vs six days DSS: 0.5 mm ± 0.2 vs nine days DSS: 0.6 mm ± 0.2, P < 0.05). Moreover, these sonographic findings correlated well with clinical parameters such as weight loss(r2 = 0.74) and histological damage(r2 = 0.86)(P < 0.01). In acute DSS-induced murine colitis, CEUS targeted against MAd CAM-1 detected and differentiated stages of mild, moderate and severe colitis via calculation of mean pixel contrast intensity in decibel(9.6 d B ± 1.6 vs 12.9 d B ± 1.4 vs 18 d B ± 3.33, P < 0.05). Employing the AOM/DSSinduced carcinogenesis model, tumor development was monitored by CEUS targeted against VEGF and detected a significantly increased echogenicity in tumors as compared to adjacent healthy mucosa(healthy mucosa, 1.6 d B ± 1.4 vs 42 d, 18.2 d B ± 3.3 vs 84 d, 18.6 d B ± 4.9, P < 0.01). Tissue echogenicity strongly correlated with histological analysis and immunohistochemistry findings(VEGF-positive cells in 10 high power fields of healthy mucosa: 1 ± 1.2 vs 42 d after DSS start: 2.4 ± 1.6 vs 84 d after DSS start: 3.5 ± 1.3, P < 0.01). CONCLUSION Molecularly targeted CEUS is a highly specific and noninvasive imaging modality, which characterizes murine intestinal inflammation and carcinogenesis in vivo. | Markus Brückner Jan Heidemann Tobias M Nowacki Friederike Cordes Jorg Stypmann Philipp Lenz Faekah Gohar Andreas Lügering Dominik Bettenworth | 2017 | World Journal of Gastroenterology2017，23，16： | 4 |
| 2 | Medium access control with coordinated adaptive sleeping for wireless sensor networks显示文摘 | Wei Ye John Heidemann Deborah Estrin | 2004 | IEEE/ACM Transactions on Networking (TON)2004，，3： | 2 |
| 3 | GPS-less Low Cost Outdoor Localization for Very Small Devices显示文摘 | Nirupama Bulusu John Heidemann Deborah Estrin | 2000 | IEEE Personal Communications2000，7，5： | 1 |
| 4 | Modeling the performance of HTTP over several transport protocols 显示文摘 | J Heidemann K Obraczka J Touch | 1997 | IEEE-ACM Trans on Networking1997，5，5： | 1 |
| 5 | Kinetics of methane hydrate decomposition显示文摘 | KIM H C BISHNOI P R HEIDEMANN R A | 1987 | Chemical Engineering Science1987，42，7： | 1 |
| 6 | Self- configuringlocalization Systems: Design and Experimental Evaluation 显示文摘 | BULUSU N HEIDEMANN J ESTRIN D | 2004 | ACM Transactions on Embedded Computing Systems2004，3，1： | 1 |
| 7 | Kinetics of methane hydrates decomposition 显示文摘 | Kim H C Bishnoi P R Heidemann R A | 1987 | Chemical Engineer- ing Science1987，42，7： | 1 |
| 8 | GPS-less Low Cost Out door Localization for very small Devices 显示文摘 | Bulusu N Heidemann J Estrin D | 2000 | IEEE Per- sonal Communications Magazine2000，7，5： | 1 |
| 9 | A chemical equilibrium equation of state model for elemental sulfur and sulfur-containing fluids显示文摘 | HEIDEMANN R A PHOENIX A V KARAN K | 2001 | Industrial & Engineering Chemistry Research2001，40，9： | 1 |
| 10 | GPS-less Low Cost Outdoor Localization for Very Small Devices显示文摘 | Bulusu N Heidemann J Estrin D | 2000 | IEEE Personal Communications Magazine2000，7，5： | 1 |
| 11 | Vehicular communication 显示文摘 | FARKAS K HEIDEMANN J IFFODE L | 2006 | IEEE Pervasive Computing2006，5，4： | 1 |
| 12 | Medium access control with coordinated adaptive sleeping for wireless sensor networks显示文摘 | Ye Wei Heidemann J Estrin D | 2004 | IEEE/ACM Transactions on Networking2004，12，3： | 1 |
| 13 | Underwater sensor networks:Applications,advances and challenges显示文摘 | Heidemann J Stojanovic M Zorzi M | 2012 | Philosophical Transactions on the Royal Society A2012，370，： | 1 |
| 14 | Influence of different pilot hole sizes on torque measurements and pull- out analysis of osteosynthesis screws显示文摘 | Heidemann W Gerlach KL Grobel KH | 1998 | J Cranio Maxill Surg1998，26，1： | 1 |
| 15 | SWATS: Wireless sensor networks for steamflood and waterflood pipeline mo- nitoring显示文摘 | Yoon S H Ye W Heidemann J | 2011 | Network IEEE2011，25，1： | 1 |
| 16 | An energy-efficient MAC protocol for wireless sensor networks显示文摘 | WEI Y HEIDEMANN J ESTRIN D | 2008 | Scientific Besearch Publishing2008，，1： | 1 |
| 17 | Why do we need a new data handling architecture for sensor networks显示文摘 | GANESAN D ESTRIN D HEIDEMANN J | 2003 | SIGCOMM Computer Communication Review2003，33，1： | 1 |
| 18 | Angiogenic effects of interleukin-8(CXCL8) in human intestinal microvascular endothelial cells are mediated by CXCR2显示文摘 | Heidemann J Ogawa H Dwinell B | 2003 | J Biol Chem2003，278，10： | 1 |
| 19 | Angiogeneic effects of interleukin 8 (CXCL8) in human intestinal microvascular endothelial calls are mediated by CXCR2显示文摘 | Heidemann J Ogawa H Dwinell MB | 2003 | J Biol Chem2003，278，10： | 1 |
| 20 | GPS-less low cost outdoor localization for very small devices显示文摘 | Bulusu N Heidemann J Estrin D | 2000 | IEEE Personal Communications Magazine2000，，： | 1 |
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