|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Can infrared thermography be used to monitor fatigue during exercise? A case study显示文摘Background: Infrared thermographic imaging(IRTG) is a safe and reliable technology used to monitor skin temperature. The main aim of our study was to evaluate the relationship between skin temperature changes and muscle fatigue in order to answer the main research question if IRTG can be used to monitor muscle fatigue.Methods: This was a case study performed on a 23-year-old trained middle distance runner. After warm-up the subject was exercising on the dynamometer for 7.5 min at 120?/s performing only concentric contractions of quadriceps. At the same time IRTG recording of both(exercising and non-exercising) quadriceps was performed.Results: A correlational analysis that was performed in order to quantify the relationship between power and temperature change over time has shown that there is a significant negative correlation between skin temperature increase and power decrease(r = -0.543, p = 0.036) of exercising quadriceps. In linear regression model the exercising quadriceps power could be predicted from skin temperature. No such relationships were noted for the non-exercising limb that served as a control.Conclusion: We believe that correlation between skin temperature change and muscle power output as described in this case study deserves further analysis on the larger sample including subjects of the different ages, health status, and physical abilities in order to create a new tool for monitoring the muscle fatigue. | Vedran Had?i Brane irok Ale? Malner?i? Milan oh | 2019 | Journal of Sport and Health Science2019，8，1： | 2 |
| 3 | 适应超低排放车和欧洲第三阶段排放标准的低排放车技术显示文摘大多数汽车制造厂正在开发能满足加州大气资源局(CARB)超低排放车法规的技术。为了使冷机起动排放(主要是HC和CO)降低到要求的水平,必须使催化器比现用的三元催化器(TWC)更早点火。带排气点火器和紧靠排气管安装的电加热型催化器(EHC)具有很大的潜力。已证实一种以EHC、热惯性小的排气岐管和先进的发动机控制技术为基础的“总系统”方法能明显减少按联邦试验规程(FTP)试验时的排放。 | Hadded,O 张耀庆 | 1996 | 国外内燃机1996，28，5： | 2 |
| 4 | Gastroprotective flavonoid constituents from Oroxylum indicum Vent显示文摘 | Had Babu T Manjulatha K Suresh Kumar G | 2010 | Bioorg Med Chem Lett2010，20，1： | 1 |
| 5 | Dynamic Window Reduction for the Multiple Depot Vehicle Scheduling Problems with Time Windows显示文摘 | HAD JAR A SOUMIS F | 2009 | Computers & Operations Research2009，36，7： | 1 |
| 6 | Population studies of sporadic cerebral amyloid angiopathy and dementia:a systematic re- view显示文摘 | Keage HAD Carare RO Friedland RP | 2009 | BMC neurology2009，9，1： | 1 |
| 7 | An Application Specific Protocol Architecture for Wire- less Microsensor Networks 显示文摘 | Wbndi Rabiner Heinzelman Anantha Chandrakasan Had Bal- akrishnan | 2002 | IEEE Transcations on Wireless Communications2002，1，4： | 1 |
| 8 | Interferon-gamma-in duced activation of indoleamine 2,3-dioxygenase in cord blood mon ocyte-derived macrophages inhibits the growth of group B strepto cocci显示文摘 | MacKenzie CR Hadding U Daubener W | 1998 | J Infect Dis1998，178，3： | 1 |
| 9 | Antioxidant activity of small grain cereals caused by phenolics and lipid soluble antioxidants显示文摘 | (Z)ili(c) S Had(z)i-Ta(s)kovi(c) (S)ukalovic V Dodig D | | 0，，03： | 1 |
| 10 | High-affinity CCK-A receptors on the vagus nerve mediate CCK-stimulated pancreatic secretion in rats显示文摘 | Li Y Had Y Owyang C | 1997 | Am J Physiol1997，273，31： | 1 |
| 11 | Effects of Gender,Birth Order,and Other Correlates on Childhood Mortality in China显示文摘 | CHOE M HAD H WANG F | 1995 | Social Biology1995，42，12： | 1 |
| 12 | Lactic acid pepsin tablets combined with compound scattered treatment of pediatric pneumonia diarrhea curative effect observation显示文摘 | Li Qing Liu Jinsong had swords and other | 2011 | Chin Med2011，14，14： | 1 |
| 13 | OpenFlow: ena- bling innovation in campus networks 显示文摘 | Nick McKeown Tom Anderson Had Balakrishnan | 2008 | ACM SIGCOMM Com- puter Communication Review2008，，4： | 1 |
| 14 | Role of IL- 1 in ischemic reperfusion injury显示文摘 | Had M Nonnan J Saba SR | 1998 | J Am Sol Nephrol1998，9，4： | 1 |
| 15 | Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms显示文摘 | Paterson A H Lander E S Had J D | 1991 | Nature1991，335，： | 1 |
| 16 | Improved reproducibility in capillary electrophoresis through the use of mobility and migration time ratios显示文摘 | YANG J BOSE S HAD S | 1996 | Chromatogr A1996，735，12： | 1 |
| 17 | A validated quantitative assay to detect occult mieremetastases by reverscriptase-polymerase chain reaction of guanylyl cyclase C in patients with colorectal eancer显示文摘 | Sehulz S Hyslop T Had J | 2006 | Clin Cancer Res2006，12，15： | 1 |
| 18 | Combination of selected biochemical markers and cervical length in the prediction of impending preterm delivery in symptomatic patients显示文摘 | Hadi-Legal M Markova AD Stefanovic M | 2016 | Clin Exp Obstet Gynecol2016，43，1： | 1 |
| 19 | A multi-scale algorithm for drawing graphs nicely 显示文摘 | HAD ANY K HAREL D | 2001 | Discrete Applied Mathematics2001，113，1： | 1 |
| 20 | Power system monitoring using Petri net embeddings显示文摘 | HAD C N VER G C | 2000 | IEE Proceedings - Generation Transmission and Distribution2000，147，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员