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| 1 | 3D Chromatin Architecture of Large Plant Genomes Determined by Local A/B Compartments显示文摘染色体的空间组织在基因表示的规定起一个重要作用。然而,核心动物染色体的结构的特征例如拓扑地联系的域(男孩) 和染色质环,不在极其紧缩的 Arabidopsis 染色体是突出的。在这研究,我们检验 ? 染色质建筑学,以及他们的 DNA methylation, histone 修正,可存取的染色质,和基因表示玉米,有染色体的西红柿,蜀黍,狐尾稷,和米饭缩放从 0.4 ? 到 2.4 Gb。我们发现这些植物染色体能被划分成象哺乳动物一样 A/B 分隔空间。在更高的分辨率,这些植物的染色体能进一步被划分到反映他们的 euchromatin, heterochromatin,和 polycomb 地位的本地 A/B 分隔空间。在所有这些植物的染色质被组织成没越过种类被保存的领域。他们显示出类似到果蝇分隔空间领域,并且被聚类进活跃, polycomb,压抑、中间的类型基于他们的 transcriptional 活动和 epigenetic 签名,与有本地 A/B 分隔空间连接的领域边阶重叠。在大玉米和西红柿染色体,我们观察了广泛的染色质环。不同于在 TAD 边阶被充实的哺乳动物的染色质环,然而种染色质环经常在压抑的域外面在基因岛之间被形成 ? 并且仔细与活跃分隔空间被联系。我们的学习显示植物有复杂、唯一的 3D 染色质体系结构,它要求 ? 阐明的进一步的学习他们的生物功能。 | Pengfei Dong Xiaoyu Tu Po-Yu Chu Peitao Lu Ning Zhu Donald Grierson Baijuan Du Pinghua Li Silin Zhong | 2017 | Molecular Plant2017，10，12： | 12 |
| 2 | Volatile composition and classification of Lilium flower aroma types and identification,polymorphisms,and alternative splicing of their monoterpene synthase genes显示文摘Lily is a well-known ornamental plant with a diversity of fragrant types.Basic information on lily floral scent compounds has been obtained for only a few accessions,and little is known about Lilium aroma types,the terpene synthase genes that may play roles in the production of key volatiles,or the range of monoterpenes that these genes produce.In this study,41 cultivars were analyzed for volatile emissions,and a total of 46 individual volatile compounds were identified,16 for the first time in lilies.Lily accessions were classified into six groups according to the composition of major scent components:faint-scented,cool,fruity,musky,fruity-honey,and lily.Monoterpenes were one of the main groups of volatiles identified,and attention was focused on terpene synthase(TPS)genes,which encode enzymes that catalyze the last steps in monoterpene synthesis.Thirty-two candidate monoterpene synthase cDNAs were obtained from 66 lily cultivars,and 64 SNPs were identified.Two InDels were also shown to result from variable splicing,and sequence analysis suggested that different transcripts arose from the same gene.All identified nucleotide substitution sites were highly correlated with the amounts of myrcene emitted,and InDel site 230 was highly correlated with the emission of all major monoterpenoid components,especially(E)-β-ocimene.Heterologous expression of five cDNAs cloned from faint-scented and strong-scented lilies showed that their corresponding enzymes could convert geranyl diphosphate to(E)-β-ocimene,α-pinene,and limonene.The findings from this study provide a major resource for the assessment of lily scent volatiles and will be helpful in breeding of improved volatile components. | Fang Du Ting Wang Jun-miao Fan Zhi-zhi Liu Jia-xin Zong Wei-xin Fan Yuan-huai Han Donald Grierson | 2019 | Horticulture Research2019，6，1： | 12 |
| 3 | 捻转针刺手法的运动学及动力学特征研究显示文摘目的:从运动学和动力学两个角度探索捻转手法的动作特征,从而为捻转手法操作的定量化提供依据。方法:运用高精度动作捕捉系统,精确测量捻转手法的幅度、频率、力量及操作时间等指标,比较12名针灸专家和12名初学者在仿真皮肤上10次操作平补平泻捻转手法时上述数据的大小及离散程度的差异。结果:专家捻转频率离散系数显著低于初学者(P<0.01),并且专家捻转前的操作时间亦显著短于初学者(P<0.01);专家与初学者捻转幅度、频率及力量的绝对数值相比,差异均无统计学意义(P>0.05)。结论:平补平泻捻转手法的技能水平并非体现在捻转的具体幅度、频率及力量上,其动作特征主要表现在动作稳定性和熟练程度两个方面;建议将捻转频率离散度和操作时间用于捻转手法的定量评估。 | 李靖 Lawrence Grierson Mary X Wu Ronny Breuer 周斌 Heather Carnahan | 2013 | 针刺研究2013，38，5： | 9 |
| 4 | A NAC transcription factor, NOR-like1, is a new positive regulator of tomato fruit ripening显示文摘Ripening of the model fruit tomato(Solanum lycopersicum)is controlled by a transcription factor network including NAC(NAM,ATAF1/2,and CUC2)domain proteins such as No-ripening(NOR),SlNAC1,and SlNAC4,but very little is known about the NAC targets or how they regulate ripening.Here,we conducted a systematic search of fruit-expressed NAC genes and showed that silencing NOR-like1(Solyc07g063420)using virus-induced gene silencing(VIGS)inhibited specific aspects of ripening.Ripening initiation was delayed by 14 days when NOR-like1 function was inactivated by CRISPR/Cas9 and fruits showed obviously reduced ethylene production,retarded softening and chlorophyll loss,and reduced lycopene accumulation.RNA-sequencing profiling and gene promoter analysis suggested that genes involved in ethylene biosynthesis(SlACS2,SlACS4),color formation(SlGgpps2,SlSGR1),and cell wall metabolism(SlPG2a,SlPL,SlCEL2,and SlEXP1)are direct targets of NOR-like1.Electrophoretic mobility shift assays(EMSA),chromatin immunoprecipitation-quantitative PCR(ChIP-qPCR),and dual-luciferase reporter assay(DLR)confirmed that NOR-like1 bound to the promoters of these genes both in vitro and in vivo,and activated their expression.Our findings demonstrate that NOR-like1 is a new positive regulator of tomato fruit ripening,with an important role in the transcriptional regulatory network. | Ying Gao Wei Wei Xiaodan Zhao Xiaoli Tan Zhongqi Fan Yiping Zhang Yuan Jing Lanhuan Meng Benzhong Zhu Hongliang Zhu Jianye Chen Cai-Zhong Jiang Donald Grierson Yunbo Luo Da-Qi Fu | 2018 | Horticulture Research2018，5，1： | 9 |
| 5 | 针刺手法操作行为的定量研究显示文摘目的:探索定量评估针刺手法操作行为的方法,并进一步分析专业针刺手法的行为特征。方法:根据针刺基本手法编制出《针刺基本手法操作行为量表》,经德尔菲法检验内容效度;2名独立评分人运用该量表评估针灸医师和初学者各12人的针刺手法操作行为,计算评判间信度,并比较这两组受试者操作行为总分及消毒、进针、行针及出针各步骤操作行为得分的差异。结果:该量表内容效度满意;评判间信度为0.768;且针灸医师组操作行为总分显著高于初学者组(13.80±1.05vs 11.03±2.14,P<0.01);医师进针及行针得分显著高于初学者(4.28±0.91vs 2.54±1.51,P<0.01;2.56±0.65vs 1.88±0.88,P<0.05);而消毒及出针得分与初学者无差异。结论:该量表可用于定量评估针刺手法操作行为,专业针刺手法的行为特征主要体现在能够完成难度较大且协调性精确性要求较高的进针及行针操作上。 | 李靖 Lawrence Grierson Mary X Wu Ronny Breuer Heather Carnahan | 2014 | 中国针灸2014，34，3： | 9 |
| 6 | Diversity and redundancy of the ripening regulatory networks revealed by the fruitENCODE and the new CRISPR/Cas9 CNR and NOR mutants显示文摘Tomato is considered as the genetic model for climacteric fruits,in which three major players control the fruit ripening process:ethylene,ripening transcription factors,and DNA methylation.The fruitENCODE project has now shown that there are multiple transcriptional circuits regulating fruit ripening in different species,and H3K27me3,instead of DNA methylation,plays a conserved role in restricting these ripening pathways.In addition,the function of the core tomato ripening transcription factors is now being questioned.We have employed CRISPR/Cas9 genome editing to mutate the SBP-CNR and NAC-NOR transcription factors,both of which are considered as master regulators in the current tomato ripening model.These plants only displayed delayed or partial non-ripening phenotypes,distinct from the original mutant plants,which categorically failed to ripen,suggesting that they might be gain-of-function mutants.Besides increased DNA methylation genome-wide,the original mutants also have hyper-H3K27me3 in ripening gene loci such as ACS2,RIN,and TDR4.It is most likely that multiple genetic and epigenetic factors have contributed to their strong non-ripening phenotypes.Hence,we propose that the field should move beyond these linear and twodimensional models and embrace the fact that important biological processes such as ripening are often regulated by highly redundant network with inputs from multiple levels. | Ying Gao Ning Zhu Xiaofang Zhu Meng Wu Cai-Zhong Jiang Donald Grierson Yunbo Luo Wei Shen Silin Zhong Da-Qi Fu Guiqin Qu | 2019 | Horticulture Research2019，6，1： | 7 |
| 7 | Combined genomic, transcriptomic, and metabolomic an alyses provide in sights into chayote (Sechium edule) evolution and fruit development显示文摘Chayote(Sechium edule)is an agricultural crop in the Cucurbitaceae family that is rich in bioactive components.To enhance genetic research on chayote,we used Nanopore third-generation sequencing combined with Hi-C data to assemble a draft chayote genome.A chromosome-level assembly anchored on 14 chromosomes(N50 contig and scaffold sizes of 8.40 and 46.56 Mb,respectively)estimated the genome size as 606.42 Mb,which is large for the Cucurbitaceae,with 65.94%(401.08 Mb)ofthe genome comprising repetitive sequences;28,237 protein-coding genes were predicted.Comparative genome analysis indicated that chayote and snake gourd diverged from sponge gourd and that a whole-genome duplication(WGD)event occurred in chayote at 25±4 Mya.Transcriptional and metabolic analysis revealed genes involved in fruit texture,pigment,fl avor,fl avonoids,antioxidants,and plant hormones during chayote fruit development.The analysis of the genome,transcriptome,and metabolome provides insights into chayote evolution and lays the groundwork for future research on fruit and tuber development and genetic improvements in chayote. | Anzhen Fu Qing Wang Jianlou Mu Lili Ma Changlong Wen Xiaoyan Zhao Lipu Gao Jian Li Kai Shi Yunxiang Wang Xuechuan Zhang Xuewen Zhang Fengling Wang Donald Grierson Jinhua Zuo | 2021 | Horticulture Research2021，8，1： | 6 |
| 8 | The persimmon(Diospyros oleifera Cheng)genome provides new insights into the inheritance of astringency and ancestral evolution显示文摘Persimmon(Diospyros kaki)is an oriental perennial woody fruit tree whose popular fruit is produced and consumed worldwide.The persimmon fruit is unique because of the hyperaccumulation of proanthocyanidins during fruit development,causing the mature fruit of most cultivars to have an astringent taste.In this study,we obtained a chromosome-scale genome assembly for‘Youshi’(Diospyros oleifera,2n=2x=30),the diploid species of persimmon,by integrating Illumina sequencing,single-molecule real-time sequencing,and high-throughput chromosome conformation capture techniques.The assembled D.oleifera genome consisted of 849.53 Mb,94.14%(799.71 Mb)of which was assigned to 15 pseudochromosomes,and is the first assembled genome for any member of the Ebenaceae.Comparative genomic analysis revealed that the D.oleifera genome underwent an ancientγwhole-genome duplication event.We studied the potential genetic basis for astringency development(proanthocyanidin biosynthesis)and removal(proanthocyanidin insolublization).Proanthocyanidin biosynthesis genes were mainly distributed on chromosome 1,and the clustering of these genes is responsible for the genetic stability of astringency heredity.Genome-based RNA-seq identified deastringency genes,and promoter analysis showed that most of their promoters contained large numbers of low oxygen-responsive motifs,which is consistent with the efficient industrial application of high CO2 treatment to remove astringency.Using the D.oleifera genome as the reference,SLAF-seq indicated that‘Youshi’is one of the ancestors of the cultivated persimmon(2n=6x=90).Our study provides significant insights into the genetic basis of persimmon evolution and the development and removal astringency,and it will facilitate the improvement of the breeding of persimmon fruit. | Qing-gang Zhu Yang Xu Yong Yang Chang-fei Guan Qiu-yun Zhang Jing-wen Huang Don Grierson Kun-song Chen Bang-chu Gong Xue-ren Yin | 2019 | Horticulture Research2019，6，1： | 4 |
| 9 | 红树林植物木榄水通道基因的克隆和表达显示文摘根据植物水通道基因保守区设计简并引物,采用RT-PCR方法,从木榄树叶中分离出水通道基因的cDNA片段;3′RACE获得3′端cDNA序列;再经5′RACE获得5′端部分cDNA序列,命名为PIP2,GenBank登录号为EF126757。该基因全长843个碱基,编码281个氨基酸,具有典型的植物水通道基因结构。该基因编码的蛋白质与含羞草(PIP2;5)、欧洲葡萄(PIP)、拟南芥(PIP3)等水通道蛋白的同源性分别为90%、91%、88%。Northern杂交分析表明,该基因在木榄树不同器官中的表达差异明显:根部有较高的表达水平,茎部较弱,而在叶中只能检测到微弱的信号。 | 祝艺懿 韩渊怀 李正国 陈国平 Donald Grierson 殷幼平 | 2007 | 植物生理学通讯2007，43，3： | 3 |
| 10 | The genome and transcriptome analysis of snake gourd provide insights into its evolution and fruit development and ripening显示文摘Snake gourd(Trichosanthes anguina L.),which belongs to the Cucurbitaceae family,is a popular ornamental and food crop species with medicinal value and is grown in many parts of the world.Although progress has been made in its genetic improvement,the organization,composition,and evolution of the snake gourd genome remain largely unknown.Here,we report a high-quality genome assembly for snake gourd,comprising 202 contigs,with a total size of 919.8 Mb and an N50 size of 20.1 Mb.These findings indicate that snake gourd has one of the largest genomes of Cucurbitaceae species sequenced to date.The snake gourd genome assembly harbors 22,874 protein-coding genes and 80.0%of the genome consists of repetitive sequences.Phylogenetic analysis reveals that snake gourd is closely related to sponge gourd but diverged from their common ancestor~33–47 million years ago.The genome sequence reported here serves as a valuable resource for snake gourd genetic research and comparative genomic studies in Cucurbitaceae and other plant species.In addition,fruit transcriptome analysis reveals the candidate genes related to quality traits during snake gourd fruit development and provides a basis for future research on snake gourd fruit development and ripening at the transcript level. | Lili Ma Qing Wang Jianlou Mu Anzhen Fu Changlong Wen Xiaoyan Zhao Lipu Gao Jian Li Kai Shi Yunxiang Wang Xuewen Zhang Xuechuan Zhang Zhangjun Fei Donald Grierson Jinhua Zuo | 2020 | Horticulture Research2020，7，1： | 2 |
| 11 | Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plant 显示文摘 | Hamilton A J Yycett G W Grierson D | 1990 | Nature1990，346，： | 2 |
| 12 | SIMYB1 and SIMYB2, two new MYB genes from tomato, transcriptionally regulate cellulose biosynthesis in tobacco显示文摘Cellulose,a major constituent of plant biomass,is synthesized by a cellulose synthase complex.It has been demonstrated that MYB genes transcriptionally regulate cellulose synthase in Arabidopsis.However,little is known about this process in tomato.Here,two MYB(SIMYB1/2) and three cellulose synthase(CESA)(SICESA4/5/6) genes were isolated.SIMYB1/2and SICESA4I5I6 accumulation was found to correspond to cellulose accumulation in different tissues of tomato.Dual luciferase assays indicated that these two MYSs were transcriptional activators that interact with promoters of SICESA4/5/6.Moreover,SIMYB2 could also activate promoters of SIMYB1/2,suggesting the possible underlying auto-activation mechanisms for MYB transcription factors.Transient over-expression of SIMYB1/2 in Nicotiana tabacum up-regulated tobacco endogenous NtCESA genes and increased cellulose accumulation.The function of SIMYB1/2 was further investigated using stable transformation and the results indicated that N.tabacum lines heterologous expressing SIMYB1/2 displayed a pleiotropic phenotype,long and narrow leaves,with NtCESA induced and significant increase of cellulose.In conclusion,our data suggest that tomato SIMYB1I2 have transcriptional regulatory roles in cellulose biosynthesis and SIMYB2 was more effective than SIMYB1,which may due to the transcriptional activation by SIMYB2 on SIMYB1 and itself. | SHI Yan-na LIU Xiao-fen LI Xue DONG Wen-cheng Donald Grierson YIN Xue-ren CHEN Kun-song | 2017 | Journal of Integrative Agriculture2017，16，1： | 2 |
| 13 | Transcriptional regulation of fleshy fruit texture显示文摘Fleshy fruit texture is a critically important quality characteristic of ripe fruit.Softening is an irreversible process which operates in most fleshy fruits during ripening which,together with changes in color and taste,contributes to improvements in mouthfeel and general attractiveness.Softening results mainly from the expression of genes encoding enzymes responsible for cell wall modifications but starch degradation and high levels of flavonoids can also contribute to texture change.Some fleshy fruit undergo lignification during development and post-harvest,which negatively affects eating quality.Excessive softening can also lead to physical damage and infection,particularly during transport and storage which causes severe supply chain losses.Many transcription factors(TFs)that regulate fruit texture by controlling the expression of genes involved in cell wall and starch metabolism have been characterized.Some TFs directly regulate cell wall targets,while others act as part of a broader regulatory program governing several aspects of the ripening process.In this review,we focus on advances in our understanding of the transcriptional regulatory mechanisms governing fruit textural change during fruit development,ripening and postharvest.Potential targets for breeding and future research directions for the control of texture and quality improvement are discussed. | Yanna Shi Bai-Jun Li Guanqing Su Mengxue Zhang Donald Grierson Kun-Song Chen | 2022 | Journal of Integrative Plant Biology2022，64，9： | 2 |
| 14 | Influences of Seed Source, Pre-chilling, Light and Temperature on the Germination of South African Pelargonium sidoides显示文摘 | F. B. Lewu D. S. Grierson A. J. Afolayan | 2010 | Journal of Agricultural Science and Technology2010，4，3： | 2 |
| 15 | Comparison among five evolutionary-based optimization algorithms显示文摘 | Emad Elbeltagi Tarek Hegazy Donald Grierson | 2005 | Advanced Engineering Informatics2005，，1： | 2 |
| 16 | Antisense gene that inhibit synthesis of hormone ethylene in transgenic plants 显示文摘 | Hamilton A J Lyett G W Grierson D | 1990 | Nature1990，346，6281： | 1 |
| 17 | Methods of detection of new family with X-linked lymphoproliferative disease显示文摘 | Purtilo DT Grierson HL | 1991 | Cancer Genet Cytogenet1991，51，3： | 1 |
| 18 | Nanotribology of Carbon - Based Materials 显示文摘 | Grierson David S Carpick Robert W | 2007 | Nano Today2007，2，5： | 1 |
| 19 | Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants 显示文摘 | Lycett GW Grierson D | 1990 | Nature1990，346，： | 1 |
| 20 | Comparison among five evolutionary-based optimization algorithms显示文摘 | Elbeltagi E Hegazy T Grierson D | 2005 | Advanced Engineering Informatics2005，19，1： | 1 |
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