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272篇 您的检索式:作者名="GRESSHOFF"
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1Molecular Analysis of Legume Nodule Development and Autoregulation显示文摘Legumes are highly important food, feed and biofuel crops. With few exceptions, they can enter into an intricate symbiotic relationship with specific soil bacteria called rhizobia. This interaction results in the formation of a new root organ called the nodule in which the rhizobia convert atmospheric nitrogen gas into forms of nitrogen that are useable by the plant. The plant tightly controls the number of nodules it forms, via a complex root-to-shoot-to-root signaling loop called autoregulation of nodulation (AON). This regulatory process involves peptide hormones, receptor kinases and small metabolites. Using modern genetic and genomic techniques, many of the components required for nodule formation and AON have now been isolated. This review addresses these recent findings, presents detailed models of the nodulation and AON processes, and identifies gaps in our understanding of these process that have yet to be fully explained.Brett J. Ferguson Arief Indrasumunar Satomi Hayashi Yu-Hsiang Lin Dugald E. Reid Peter M. Gresshoff 2010Journal of Integrative Plant Biology2010,52,1:23
2A Novel ABA Insensitive Mutant of Lotus japonicus with a Wilty Phenotype Displays Unaltered Nodulation Regulation显示文摘骆驼毛的织物感觉迟钝的异种, Beyma,作为屏蔽标准用根生长抑制从一张他们 mutagenesis 人口在莲花 japonicus MG-20 被孤立到应用骆驼毛的织物。(名字 Beyma 从澳大利亚的土著的语言被拿, Wagiman, beyma,意思枯竭) 因为一个主导的孟德尔的变化对骆驼毛的织物感觉迟钝,分离的稳定的异种导致了萌芽,植物的生长,有气孔的洞,以及生节的抑制。织物骆驼毛的织物层次是正常的,建议敏感而非生合成变化。它是慢成长的(5070% 野类型的 MG-20 ) 并且在组成附近的 wilty 显型与它的无能联系了调整有气孔的开。虽然显示出大量骆驼毛的织物感觉迟钝的显型,, Beyma 没当增加的骆驼毛的织物,数字和 patterning 不在时显示出小瘤数字控制的改变(然而并非尺寸) 在异种形成的小瘤类似于 MG-20 的。MG-20 的裂口根实验证明根的一个方面上的骆驼毛的织物的申请局部地然而并非全身地禁止了生节。我们建议那件骆驼毛的织物不直接涉及生节(AON ) 的全身的自动调整。Bandana Biswas Pick Kuen Chan Peter M Gresshoff 2009Molecular Plant2009,2,3:4
3Classical Ethylene Insensitive Mutants of the Arabidopsis EIN2 Orthologue Lack the Expected‘hypernodulation’ Response in Lotus japonicus显示文摘Three independent ethylene insensitive mutants were selected from an EMS-mutagenized population of Lotus japonicus MG-20 (Miyakojima). The mutants, called 'Enigma', were mutated in the LjEIN2a gene from Lotus chromosome 1, sharing significant homology with Arabidopsis EIN2 (ethylene-insensitive2). All three alleles showed classical ethylene insensitivity phenotypes (e.g., Triple Response), but lacked the increased nodulation phenotype commonly associated with ethylene insensitivity. Indeed, all showed a marginal reduction in nodule number per plant, a phenotype that is enigmatic to sickle, an ethylene-insensitive EIN2 mutant in Medicago truncatula. In contrast to wild type, but similar to an ETR1-1 ethylene ethylene-insensitive transgenic of L. japonicus, enigma mutants formed nodules in between the protoxylem poles, demonstrating the influence of ethylene on radial positioning. Suppression of nodule numbers by nitrate and colonisation by mycorrhizal fungi in the enigma-1 mutant were indistinguishable from the wild type MG-20. However, reflecting endogenous ethylene feedback, the enigma-1 mutant released more than twice the wild-type amount of ethylene. enigma-1 had a moderate reduction in growth, greater root mass (and lateral root formation), delayed flowering and ripening, smaller pods and seeds. Expression analysis of ethylene-regulated genes, such as ETR1, NRL1 (neverripe-like 1), and EIL3 in shoots and roots of enigma-1 and MG-20 illustrated that the ethylene-insensitive mutation strongly affected transcriptional responses in the root. These mutants open the possibility that EIN2 in L. japonicus, a determinate nodulating legume, acts in a more complex fashion possibly through the presence of a duplicated copy of LjEIN2.Pick Kuen Chan Bandana Biswas Peter M. Gresshoff 2013Journal of Integrative Plant Biology2013,55,4:2
4Fast and sensitive silver staining of DNA in polyacrylamide gels 显示文摘Bassam B J Caetano-Anolles G Gresshoff P M 1991Anal Biochem1991,196,1:1
5Fast and sensitive silver staining of DNA in polyacryamid gels显示文摘Bassam B J Caetano A G Gresshoff P M 1991Anal Biochem1991,196,1:1
6Sliver staining DNA in polyayl- amide gels显示文摘BASSM B J GRESSHOFF P M 2007Nature Protoc2007,2,:1
7Fast and sensitive silver staining of DNA in polyacrylamide gels显示文摘BASSAM B J CAETANO G GRESSHOFF P M 1991Analytical Biochemistry1991,196,1:1
8Fast and sensitive silver staining of DNA in polyacrylamide gels显示文摘Bassam BJ Anolles GC Gresshoff PM 1991Anal Biochem1991,196,1:1
9Staining nucleic acids with silver:an alternative to radioisotopic and fluorescent labeling显示文摘CAETANO-ANOLLES G GRESSHOFF P M 1994Promega Notes Magazine1994,45,:1
10Fast and sensitive silver staining of DNA in polyacryamid gels显示文摘Bassam B J Caetano A G Gresshoff P M 1991Analytical Biochemistry1991,196,:1
11Fast and sensitive staining of DNA in polyacrylamide gels显示文摘Bassam BJ Caetano-Anolles G Gresshoff PM 1991Anal Biochem1991,196,1:1
12Fast and sensitive silver staining of DNA in polyacry lamide gels 显示文摘Bassam B J Caetano-Anolles G Gresshoff P M 1991Analytical Biochemistry1991,196,1:1
13Fast and sensitive silver staining of DNA in polyacrylamide gels 显示文摘Bassam BJ Caetano Anolles G Gresshoff PM 1991Anal Biochem1991,196,1:1
14Search for molecular markers of salt tolerance of soybean by DNA amplifyication fingerprinting显示文摘Zhong M Hu Z A Gresshoff P M 1997Soybean Genetics Newsletter1997,24,:1
15Fast and sensitive silver staining of DNA in polyacrylamide gels 显示文摘Bassam BJ Caetano-Anolles G Gresshoff PM 1991Anal Biochem1991,196,1:1
16Fast and sensitive silver staining of DNA in polyacrylamide gels显示文摘Bassam B J Caetano-Anolles G Gresshoff P M 1991Analytical Biochemistry1991,196,1:1
17Development and diferentiation of haploid Lycopersicon esculentum (tomato) 显示文摘GRESSHOFF P M DOY C H 1972Planta1972,107,:1
18Fast and sensitive silver staining of DNA in polyacrylamide gels显示文摘Bassam B J Anolles G C Gresshoff P M 1991Anal Biochem1991,196,1:1
19Fast and sensitive silver staining of DNA in polyacrylamide gels 显示文摘Bassam B J Caetano-Anolles G Gresshoff P M 1991 1991Annals of Biochemistry1991,80,:1
20Fast and sensitive silver straining of DNA in polyacrylamide gels显示文摘Bassam BJ Caetano-Anolles G Gresshoff PM 1991Anal Biochem1991,196,1:1
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