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| 1 | Lipopolysaccharide induces and activates the Nalp3 inflammasome in the liver显示文摘AIM:To examine the activation of the Nalp3 inflammasome and its downstream targets following lipopolysaccharide(LPS) -induced stimulation in the liver. METHODS:Six-to-eight-week-old C57BL/6 chow fed mice were injected intraperitoneally with 0.5μg/g bodyweight LPS and sacrificed 2,4,6,18 or 24 h later. LPS-induced liver damage was confirmed by a biochemical assay to detect alanine aminotransferase(ALT) levels.To determine if LPS stimulation in the liver led to activation of the inflammasome,real-time quantitative polymerase chain reaction was used to evaluate the mRNA expression of components of the Nalp3 inflammasome.Enzyme-linked immunosorbent assays were used to determine the protein expression levels of several downstream targets of the Nalp3 inflammasome,including caspase-1 and two cytokine targets of caspase-1,interleukin(IL) -1βand IL-18. RESULTS:We found that LPS injection resulted in liver damage as indicated by elevated ALT levels.This was associated with a significant increase in both mRNA and protein levels of the proinflammatory cy-tokine tumor necrosis factor(TNF) -αin the liver,as well as increased levels of TNFs in serum.We showed that LPS stimulation led to upregulation of mRNA levels in the liver for all the receptor components of the inflammasome,including Nalp3,Nalp1,pannexin-1 and the adaptor molecule apoptosis-associated specklike,caspase recruitment domain-domain containing protein.We also found increased levels of mRNA and protein for caspase-1,a downstream target of the inflammasome.In addition,LPS challenge led to increased levels of both mRNA and protein in the liver for two cytokine targets of caspase-1,IL-1βand IL-18. Interestingly,substantial baseline expression of pre-IL1βand pre-IL-18 was found in the liver.Inflammasome and caspase-1 activation was indicated by the significant increase in the active forms of IL-1βand IL-18 after LPS stimulation. CONCLUSION:Our results show that the Nalp3 inflammasome is upregulated and activated in the liver in response to LPS stimulation. | Michal Ganz Timea Csak Bharath Nath Gyongyi Szabo | 2011 | World Journal of Gastroenterology2011，17，43： | 14 |
| 2 | High fat diet feeding results in gender specific steatohepatitis and inflammasome activation显示文摘AIM:To develop an animal model that encompasses the different facets of non-alcoholic steatohepatitis(NASH),which has been a challenge.METHODS:In this study,we used a high fat diet(HFD)feeding supplemented with fructose and sucrose in the water mimicking the high-fructose corn syrup that is abundant in the diet in the United States.We used C57Bl/6 wild-type mice for short and long-term feedings of 6 and 16 wk respectively,and evaluated the extent of liver damage,steatosis,and inflammasome activation.Our methods included histopathological analysis to assess liver damage and steatosis,which involved H and E and oil-red-o staining;biochemical studies to look at ALT and triglyceride levels;RNA analysis using quantitative polymerase chain reaction;and cytokine analysis,which included the enzyme-linked immunosorbent assay method to look at interleukin(IL)-1βand tumor necrosis factor-α(TNFα)levels.Furthermore,at each length of feeding we also looked at insulin resistance and glucose tolerance using insulin tolerance tests(ITT)and glucose tolerance tests.RESULTS:There was no insulin resistance,steatosis,or inflammasome activation at 6 wk.In contrast,at16 wk we found significant insulin resistance demonstrated by impaired glucose and ITT in male,but not female mice.In males,elevated alanine aminotransferase and triglyceride levels,indicated liver damage and steatosis,respectively.Increased liver TNFαand monocyte chemoattractant protein-1 mRNA and protein,correlated with steatohepatitis.The inflammasome components,adaptor molecule,Aim2,and NOD-like receptor 4,increased at the mRNA level,and functional inflammasome activation was indicated by increased caspase-1 activity and IL-1βprotein levels in male mice fed a long-term HFD.Male mice on HFD had increasedα-smooth muscle actin and pro-collagen-1 mRNA indicating evolving fibrosis.In contrast,female mice displayed only elevated triglyceride levels,steatosis,and no fibrosis.CONCLUSION:Our data indicate gender differences in NASH.Male mice fed a long-term HFD display steatohepatitis and inflammasome activation,whereas female mice have steatosis without inflammation. | Michal Ganz Timea Csak Gyongyi Szabo | 2014 | World Journal of Gastroenterology2014，20，26： | 8 |
| 3 | 终末期肾病患者大血管和微血管内皮功能障碍与亚临床心功能不全的关系显示文摘终末期肾病(end-stagerenaldisease,ESRD)患者的心脏衰竭和心血管病死亡率较高,而我们对此缺乏深入透彻的了解,是否存在可变因素导致了这些高危患者发生心功能不全。为了评估血管内皮功能能否作为ESRD患者潜在的心功能不全的可变因素,研究人员应用横断面研究在一个控制良好的ESRD队列中,分析大血管和微血管功能障碍与左、右心功能不全的相关性。 | 刘莉 叶鹏 Dubin RF Guajardo I Ayer A Mills C Donovan C Beussink L Scherzer R Ganz P Shah SJ | 2016 | 中华高血压杂志2016，24，9： | 5 |
| 4 | Immune and inflammatory pathways in NASH显示文摘 | Michal Ganz Gyongyi Szabo | 2013 | Hepatology International2013，，2： | 2 |
| 5 | Defensins and host defense 显示文摘 | Ganz T | 1999 | Science1999，286，5439： | 2 |
| 6 | 评定药物治疗对生活质量影响的方法显示文摘测定与健康有关的生活质量的方法来源于社会科学的早期研究,最近已将这些方法用于评定接受医疗的人中与健康有关的生活质量。本文对下列问题作了综述:生活质量的定义;选择评定方法时一般考虑的问题;现有的评定法;可行性和质量控制问题;生活质量评价与对药物治疗的经典评价的关系。 | Ganz PA 程违 | 1991 | 国外医学(药学分册)1991，18，1： | 2 |
| 7 | Defensins: antimicrobial peptides of innate immunity显示文摘 | Ganz T | 2003 | Nat Rev Immunol2003，3，9： | 1 |
| 8 | Magnetic resonance imaging of the normal and ischemic femoral head 显示文摘 | Totty WG Murphy WA Ganz WI | 1984 | AJR Am J Roentgenol1984，143，6： | 1 |
| 9 | Application of infrared spectroscopy to the classification of kerogen types and the evolution of source rock and oil-shale potentials显示文摘 | GANZ H KALKREUTH W | 1987 | Fuel1987，66，5： | 1 |
| 10 | Early lesions of the labrum and acetabular cartilage in osteonecrosis of the femoral head 显示文摘 | Kloen P Leunig M Ganz R | 2002 | J Bone Joint Surg Br2002，84，： | 1 |
| 11 | Lightpath Communications: an Approach to High Bandwidth Optical WANs显示文摘 | Chlamtac I Ganz A Karmi G | 1992 | IEEE Trans on Communications1992，40，7： | 1 |
| 12 | Monocyte-chemotactic activity of defensins from hunan neulrophils显示文摘 | Territo MC Ganz T Seleted ME | 1989 | J Chin lnvesl1989，84，6： | 1 |
| 13 | Defensins and other antimicrohial peptides: a historical perspective and an update 显示文摘 | GANZ T | 2005 | Comb Chem High Throughput Screen2005，8，： | 1 |
| 14 | Arm edema in breastcancer patients显示文摘 | Rickson VS Pearson MI Ganz PA | 2001 | Natl Cancer Inst2001，93，2： | 1 |
| 15 | Defensins: antimicrobial and cytoxic peptides of mammalian cells显示文摘 | Lehrer RI Lichtenstein AK Ganz T | 1993 | Annu Rev Immunol1993，11，： | 1 |
| 16 | Defensins: antimicrobial and cytoxic peptides of mammalian cells显示文摘 | Lehrer RI Lichtenstein AK Ganz T | 1993 | Annu Rev Immunol1993，11，： | 1 |
| 17 | Dynamic study of the physical processes in the intrinsic line electromigration of deep-submicron copper and aluminum interconnects 显示文摘 | Tan C M ZhangG GanZ H | 2004 | IEEE Transactions on Device and Materials Reliability2004，4，3： | 1 |
| 18 | Influence of the heating rate on the microstructure and macroscopic properties of sol-gel SnO2:Sb coating显示文摘 | GANZ P J GASPARROG D | 1998 | Sol-Gel Sci Technol1998，13，： | 1 |
| 19 | Increased bronchoalveolar lavage human beta-defensin type 2 in bronchiolitis obliterans syndrome after lung transplantation显示文摘 | Yoshioka D Laks H Ganz T | 2004 | Transplantation2004，78，8： | 1 |
| 20 | Indirect reduction with a condylar blade plate for osteosynthesis of subtrochanteric femoral fractures显示文摘 | Siebenrock KA Muller U Ganz R | 1998 | Injury1998，29，3： | 1 |
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