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| 1 | Anti-tumor necrosis factor α therapy associates to type 17 helper T lymphocytes immunological shift and significant microbial changes in dextran sodium sulphate colitis显示文摘BACKGROUND Anti-tumor necrosis factor α(TNFα) represents the best therapeutic option to induce mucosal healing and clinical remission in patients with moderate-severe ulcerative colitis. On the other side gut microbiota plays a crucial role in pathogenesis of ulcerative colitis but few information exists on how microbiota changes following anti-TNFα therapy and on microbiota role in mucosal healing.AIM To elucidate whether gut microbiota and immune system changes appear following anti TNFα therapy during dextran sulfate sodium(DSS) colitis.METHODS Eighty C57 BL/6 mice were divided into four groups: 'No DSS', 'No DSS + antiTNFα', 'DSS' and 'DSS + anti-TNFα'. 'DSS' and 'DSS + anti-TNFα' were treated for 5 d with 3% DSS. At day 3, mice whithin 'No DSS+anti-TNFα' and'DSS+anti-TNFα' group received 5 mg/kg of an anti-TNFα agent. Forty mice were sacrificed at day 5, forty at day 12, after one week of recovery post DSS. The severity of colitis was assessed by a clinical score(Disease Activity Index), colon length and histology. Bacteria such as Bacteroides, Clostridiaceae, Enterococcaceae and Fecalibacterium prausnitzii(F. prausnitzii) were evaluated by quantitative PCR.Type 1 helper T lymphocytes(Th1), type 17 helper T lymphocytes(Th17) and CD4+ regulatory T lymphocytes(Treg) distributions in the mesenteric lymph node(MLN) were studied by flow cytometry.RESULTS Bacteria associated with a healthy state(i.e., such as Bacteroides, Clostridiaceae and F. prausnitzii) decreased during colitis and increased in course of anti-TNFαtreatment. Conversely, microorganisms belonging to Enterococcaceae genera,which are linked to inflammatory processes, showed an opposite trend.Furthermore, in colitic mice treated with anti-TNFα microbial changes were associated with an initial increase(day 5 of the colitis) in Treg cells and a consequent decrease(day 12 post DSS) in Th1 and Th17 frequency cells. Healthy mice treated with anti-TNFα showed the same histological, microbial and immune features of untreated colitic mice. 'No DSS + anti-TNFα' group showed a lymphomononuclear infiltrate both at 5 th and 12 th d at hematoxylin and eosin staining, an increase of in Th1 and Th17 frequency at day 12, an increase of Enterococcaceae at day 5, a decrease of Bacteroides and Clostridiaceae at day 12.CONCLUSION Anti-TNFα treatment in experimental model of colitis improves disease activity but it is associated to an increase in Th17 pathway together with gut microbiota alteration. | Valentina Petito Cristina Graziani Loris R Lopetuso Marco Fossati Alessandra Battaglia Vincenzo Arena Domenico Scannone Gianluca Quaranta Andrea Quagliariello Federica Del Chierico Lorenza Putignani Luca Masucci Maurizio Sanguinetti Alessandro Sgambato Antonio Gasbarrini Franco Scaldaferri | 2019 | World Journal of Gastroenterology2019，25，12： | 2 |
| 2 | Executive functioning in unipolardep ression:a review显示文摘 | Fossati P Ergis AM Allilaire JF | 2002 | Encephale2002，28，2： | 1 |
| 3 | Randomized study of adjuvant chemotherapy for completly resected stage Ⅰ,Ⅱ,or Ⅲ A non-smallcell Lung cancer显示文摘 | Scagliotti GV Fossati R Torri V | 2003 | J Natl Cancer Inst2003，95，19： | 1 |
| 4 | Term Amniotic membraneis a high throughput source for multipotent Mesenchymal StemCells with the ability to differentiate into endothelial cells invitro 显示文摘 | Alviano F Fossati V Marchionni C | 2007 | BMC Dev Biol2007，7，： | 1 |
| 5 | Inhibition of Poly(ADP-Ribose) glycohydrolase by gallotannin selectively up-regulates expression of proinflammatory genes显示文摘 | Rapizzi E Fossati S Moroni F | 2004 | Mol Pharmacol2004，66，4： | 1 |
| 6 | Temperament,character,and attachment patterns in borderline personality disorder显示文摘 | Fossati A Donati D Donini M Novella L Bagnato M Maffei C | 2001 | J Personal Disord2001，15，： | 1 |
| 7 | The antitumor histone deacety-lase inhibitor suberoylanilide hydroxamic acid exhibits anti-in-flammatory properties via suppression of cytokines显示文摘 | Leoni F Zaliani A Bertolini G Porro G Pagani P Pozzi P Dona G Fossati G Sozzani S* Azam T Bufler P Fantuzzi G Goncharov I Kim SH Pomerantz BJ Reznikov LL SiegmundB Dinarello CA Mascagni P | 2002 | ProcNatl Acad Sci USA2002，99，： | 1 |
| 8 | High speed railway:collecting pantographs active control and overhead lines diagnostic solution显示文摘 | Diana G Fossati F Resta F | 1998 | Vehicle System Dynamics1998，30，： | 1 |
| 9 | Fatal vancomycin- and linezol- id-resistant Enterococcus faecium sepsis in a child undergoing allo- geneic haematopoietic stem cell transplantation for beta-thalassae- mia major 显示文摘 | Fossati M CappeUi B Biral E | 2010 | J Med Microbiol2010，59，7： | 1 |
| 10 | beta-Interferon therapy of chronic hepatitis HCV+,1b genotype显示文摘 | Vezzoli M Girola S Fossati G | 1998 | Recenti Prog Med1998，89，5： | 1 |
| 11 | Cognitive control andbrain resources in major depression : an fMRI study using the n-back task显示文摘 | Harvey PO Fossati R Poehon JB | 2005 | Neuroimage2005，26，3： | 1 |
| 12 | The histone deacetylase inhibitor ITF2357 reduces production of pro-inflammatory cytokines in vitro and systemic in- flammation in vivo 显示文摘 | LEONI F FOSSATI G LEWIS E C | 2005 | Molecular Medicine2005，11，123456789101112： | 1 |
| 13 | Glow-Discharge Nitriding and Post-Oxidising Treatments of AISI H11Steel显示文摘 | Borgioli F Galvanetto E Fossati A | 2002 | Surface and Coatings Technology2002，162，1： | 1 |
| 14 | Executive functioning in unipolar depression: a review 显示文摘 | Fossati P Ergis AM Allilaire JF | 2002 | Encephale2002，28，2： | 1 |
| 15 | Term amniotic membrane is a high throughput source for multipotent mesenchymal stem cells with the ability to differentiate into endothelial cells in vitro 显示文摘 | Alviano F Fossati V Marchionni C | 2007 | BMC Dev Biol2007，21，1： | 1 |
| 16 | Genetic relationships and clonal identity in a collection of commercially relevant poplar cultivars assessed by AFLP and SSR 显示文摘 | Fossati T Zapelli I Bisoffi S | 2005 | Tree Genetics & Genomes2005，1，： | 1 |
| 17 | Fcgamma receptors in autoimmune diseases显示文摘 | Fossati G Bucknall RC Edwards SW | 2001 | Eur J Clin Invest2001，31，9： | 1 |
| 18 | Psyehologieal str- ess in nurses' relationships with H1V-infeeted patients:the risk of burmout syndrome显示文摘 | Visintini R Campanlni E Fossati A | 1996 | AIDS Care1996，8，2： | 1 |
| 19 | Impact of overhead line irregularity on current collection and diagnostics based on the measurement of pantograph dynamics显示文摘 | COLLINA A FOSSATI F PAPI M | 2007 | Proceedings of the Institution of Mechanical Engineers Part F: Journal of Rail and Rapid Transit2007，221，4： | 1 |
| 20 | Pulmonary hemosiderosis induced by cow' s milk proteins: a discussion of a clinical case 显示文摘 | Fossati G Perri M Careddu G | 1992 | Pediatr Med Chir1992，14，： | 1 |
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