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| 1 | Chip-based digital PCR as a novel detection method for quantifying microRNAs in acute myocardial infarction patients显示文摘miRNAs 为尖锐心肌的梗塞(AMI ) 作为潜在的 biomarkers 显示出诺言。然而,当前的使用的量的即时 PCR (qRT-PCR ) 完全为 nucleic 酸的相对表示允许,它产生日常可变性,它限制了把 miRNAs 用作 biomarkers 的有效性。在这研究,我们探索了技术质量和一种新技术的诊断潜力,基于薄片的数字 PCR,在确定在有 AMI 和 ischaemia-reperfusion 损害(I/R ) 的病人的 miRNAs。在合成 C.elegans-miR-39 的一个冲淡系列,基于薄片的数字 PCR 与 qRT-PCR 相比显示了变化(8.9% 对 46.3%) 和察觉(0.2 copies/L 对 1.1 copies/L ) 的更低的限制的一个更低的系数。在从有圣举起心肌的梗塞( STEMI )的 24 个病人和有稳定的冠的动脉疾病( CAD )的 20 个病人镇定的浆液在经皮的冠的干预(一种总线标准)以后的病人,我们使用了 qRT-PCR 和多路的基于薄片的数字 PCR 确定他们在优先的研究在 AMI 被验证了的 miRNA-21 和 miRNA-499 的浆液层次。在 STEMI, I/R 损害经由圣片断分辨率(ST-R ) 的测量被估计。基于薄片的数字 PCR 处于在稳定的 CAD 和 STEMI 组之间的 miR-21 层次的差别揭示了统计意义(118.8 copies/L 对 59 copies/L;P=0.0300 ) ,而 qRT-PCR 是不能的到达意义(136.4 copies/L 对 122.8 copies/L;P=0.2273 ) 。为 miR-499 层次,基于薄片的数字 PCR 和 qRT-PCR 揭示了稳定的 CAD 和 STEMI 组之间的统计上重要的差别(2 copies/L 对 8.5 copies/L, P=0.0011;0 copies/L 对 19.4 copies/L;P < 0.0001 ) 。在 miR-21/499 层次和 ST-R 之间没有协会一种总线标准以后。我们的结果证明基于薄片的数字 PCR 展出优异技术质量和诺言是一个优异方法因为确定的 miRNA 在发行量铺平,它可以为直接确定成为一个更精确、可再现的方法 miRNAs,特别地为在大多中心的使用临床的试用。 | Samuel ROBINSON Marie FOLLO David HAENE Maximilian MAULER Daniela STALLMANN Lukas Andreas HEGER Thomas HELBING Daniel DUERSCHMIED Karlheinz PETER Christoph BODE Ingo AHRENS Marcus HORTMANN | 2018 | Acta Pharmacologica Sinica2018，39，7： | 5 |
| 2 | Renal impairment after spontaneous bacterial peritonitis in cirrhosis: incidence、clinical course,predictive factors and prognosis显示文摘 | Follo A Llovet JM Navasa M | 1994 | Hepatology1994，20，： | 1 |
| 3 | Association of STATs with relatives and friends显示文摘 | Moitreyee C K Follo V D A George R S | 2000 | Trend Cell Biol2000，10，： | 1 |
| 4 | Renal impairment following spontaneous bacterial peritonitis in cirhhosis:incidence,clinical course,predictive factors and prognosis显示文摘 | Follo A Llovet JM | 1994 | Hepatology1994，20，： | 1 |
| 5 | Association of STATs with relatives and friends显示文摘 | Moitreyee CK Follo VDA George RS | 2000 | Trend Cell Biology2000，10，3： | 1 |
| 6 | The Akt/mammalian target of rapamycin signal transduction pathway is activated in high-risk myelodysplastic syndromes and influences cell survival and proliferation显示文摘 | Follo MY Mongiorgi S Bosi C | | 0，，9： | 1 |
| 7 | Renal impairment after spontaneous bacterial peritonitis in cirrhosis:incidence,clinical course,predictive factors and prognosis显示文摘 | Follo A Llovet JM Navasa M | 1994 | Hepatology1994，20，： | 1 |
| 8 | Structure and expression of the human gene encoding plasminogen activator inhibitor,PAl-1显示文摘 | Follo M Ginsburg D | 2003 | Gene2003，84，： | 1 |
| 9 | Some remarks on entropy numbers of sets with non-integer dimension显示文摘 | FOLLO G | 2007 | Ricerche di Matematica2007，56，： | 1 |
| 10 | Inhibition of PI3k class Ⅲ-dependent autophagy prevents apoptosis and necrosis by oxidative stress in dopaminergic neuroblastoma cells显示文摘 | Castino R Bellio N Follo C | 2010 | Toxieol Sci2010，117，1： | 1 |
| 11 | Tumor necrosis factor and interleukin-6 in spontaneous bacterial peritonitis in cirrhosis:relationship with the development of renal imapirment and mortality显示文摘 | Navasa M Follo A Jimenez W Francitorra A Planas R Rimola A Arroyo V Rodes J | | 0，，： | 1 |
| 12 | Real-time PCR as a tool for quantitative analysis of PI-PLC beta-1 gene expression in myelodysplastic syndrome显示文摘 | FOLLO MY BOSI C FINELLI C | 2006 | International Journal of Molecular Medicine2006，18，2： | 1 |
| 13 | Conglomerates as clues to the sedimentary and tectonic evolution of a suspect terrane: Wallowa Mountains, Oregon显示文摘 | Follo M F | 1992 | Geological Society of America Bulletin1992，104，： | 1 |
| 14 | Tumor necrosis factor and interleukin-6 in spontaneous bacterial peritonitis in cirrhosis:relationship with the development of renal impairment and mortality 显示文摘 | Navasa M Follo A Filella X | 1998 | Hepatology1998，27，： | 1 |
| 15 | Randomized comparative study of oral ofloxacin versus intravenous cefotaxime in spontaneous bacterial peritonitis 显示文摘 | Follo A Llovet JM | 1996 | Gastroenterology1996，111，4： | 1 |
| 16 | 显示文摘 | Navasa M Follo A Filella X | 1998 | Hepatology1998，27，5： | 1 |
| 17 | Tumor necrosis factor and Interleukin-6 inspontaneous bacterial peritonitis in cirrhosis:ralationship with the development of renal impairment and mortality显示文摘 | Navasa M Follo A Filella X | 1998 | Hepatology1998，27，5： | 1 |
| 18 | Side population cells from different precursor compartments显示文摘 | Guo Y Follo M Geiger K | 2003 | J Hematother Stem Cell Res2003，12，1： | 1 |
| 19 | Daily norfloxacin is more effective than wekly rufloxacin in prevention of spontaneous bacterial peritonitis recurrence显示文摘 | Bauer TM Follo A Navass M | 2002 | Dig Dis Sci2002，47，6： | 1 |
| 20 | Association of STATs with relatives and friends 显示文摘 | Moitreyee CK Follo VDA George RS | 2000 | Trend Cell Biology2000，10，： | 1 |
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