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| 1 | Clinicopathologic features, diagnosis and surgical treatment of intrahepatic cholangiocarcinoma in 104 patients显示文摘BACKGROUND: The outcome of surgical treatment of pa- tients with intrahepatic cholangiocarcinoma (ICC) is poor. This study was designed to analyze the relationship between clinicopathologic features and the survival time after opera- tion. METHODS: The operation was performed in 104 patients with mass-forming type ICC at our hospital between No- vember 1996 and May 2000. Seventy-nine patients (76.0%) were followed up successfully. Sixteen clinicopathological variables including age, sex, history of chronic liver di- sease , HBsAg, operation, adjuvant therapy, ascites, lymph node metastasis, invasion of adjacent organs, tumor size, necrosis of tumor, envelope, intrahepatic metastasis, Inter- national Union Against Cancer (UICC) TNM staging, his- tology, and cirrhosis were selected for univariate and multi- variate analyses to evaluate their influence on the prognosis. RESULTS: The accumulative 1-, 3-, 5-year survival rates of the 79 patients were 49.4%, 17.3%, 9.6% respectively. Univariate analysis revealed that sex (P=0.0221), HBsAg (P=0.0115), operation (P=0.0042), adjuvant therapy (P= 0.0389), ascites (P=0.0001), invasion (P=0.0220), intra- hepatic metastasis (P=0.0000) and TNM stage (P= 0.0001) were related to survival time. Multivariate analysis revealed that HBsAg, ascites and TNM stage were signifi- cantly related to prognosis. CONCLUSION: Early diagnosis and treatment and major hepatectomy are essential to improving the results of surgi- cal treatment of ICC patients. | Xiao-Hui Fu, Zhao-Hui Tang, Ming Zong, Guang-Shun Yang, Xiao-Ping Yao and Meng-Chao Wu Shanghai, China Department of Comprehensive Therapy of Hepatobi- liary Tumors, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，2： | 24 |
| 2 | Multi-slice three-dimensional spiral CT cholangiography: a new technique for diagnosis of biliary diseases显示文摘Objective: To validate multi-slice three-dimensionalspiral CT cholangiography (3-D CTC) in clinical di-agnosis of biliary diseases.Methods: This study included 146 patients with bili-ary diseases, involving 73 cases of biliary tumor, 87cases of radioparent calculus, 12 cases of post cholan-gio-jejunostomy and one case of congenital choledo-chocyst. The data of thin-slice volumetric CT scanwere sent to the workstation (GE Advantage Win-dows 3. 1). Rational 3-D CTC including maximumintensity projection, minimum intensity projection,surface shaded display, CT virtual endoscopy andray sumption was performed. The diagnostic accura-cy of 3-D CTC was compared with that of conven-tional CT, ultrasonography and endoscopic retro-grade cholangiopancreaticography (ERCP).Results: Different biliary diseases showed distinct ima-ging manifestations on 3-D CTC, As a new techniquefor assessing the status of post cholangio-jejunosto-my, 3-D CTC was superior to conventional CT, ul-trasonography and ERCP in diagnosis of negative bil-iary calculus, extrahepatic cholangiocarcinoma,cancer embolus of the biliary duct, carcinoma of thepancreas head and periampullar carcinoma. It wasalso superior to conventional CT, ultrasonography orequal to ERCP in diagnosis of hilar cholangiocarcino-ma, but inferior to conventional CT and ultrasonog-raphy in diagnosis of gallbladder cancer.Conclusion: 3-D CTC as a non-invasive and sensitivetechnique for the diagnosis of biliary diseases withhigh diagnostic accuracy will greatly increase the de-tection rate of biliary diseases. | Ai-Min Xu Hong-Yan Cheng Wen-Bin Jiang Dong Chen Yu-Chen Jia Meng-Chao Wu the Department of Radiology, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China | 2002 | Hepatobiliary & Pancreatic Diseases International2002，1，4： | 22 |
| 3 | Risk factors of intrahepatic cholangiocarcinoma in patients with hepatolithiasis:a case-control study显示文摘BACKGROUND:Why 3.3% to 10% of all patients with hepatolithiasis develop intrahepatic cholangiocarcinoma (ICC) remains unknown.We carried out a hospital-based case-control study to identify risk factors for the development of ICC in patients with hepatolithiasis in China.METHODS:Eighty-seven patients with pathologically diagnosed hepatolithiasis associated with ICC and 228 with hepatolithiasis alone matched by sex,age (±2 years),hospital admittance and place of residence were interviewed during the period of 2000-2008.Odds ratios (OR) and 95% confidence intervals (CI) were calculated for each risk factor.RESULTS:Among the patients with hepatolithiasis associated with ICC,the mean age was 57.7 years and 61.0% were female Univariate analysis showed that the significant risk factors for ICC development in hepatolithiasis were smoking,family history of cancer,appendectomy during childhood (under age 20),and duration of symptoms >10 years.In multivariate stepwise logistic regression analysis,smoking (OR=1.931,95% CI:1.000-3.731),family history of cancer (OR=5.175,95% CI:1.216-22.022),and duration of symptoms >10 years (OR=2.348 95% CI:1.394-3.952) were independent factors.CONCLUSION:Smoking,family history of cancer and duration of symptoms >10 years may be risk factors for ICC in patients with hepatolithiasis. | Zhen-Yu Liu,Yan-Ming Zhou,Le-Hua Shi and Zheng-Feng YinAuthor Affiliations:Molecular Oncology Laboratory and Department of Comprehensive Treatment I,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Shanghai 200438,China Department of Hepato-Biliary-Pancreato-Vascular Surgery,First Affiliated Hospital,Xiamen University,Xiamen 361003,China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，6： | 21 |
| 4 | Changes of serum alpha-fetoprotein and alpha-fetoprotein-L3 after hepatectomy for hepatocellular carcinoma: prognostic significance显示文摘BACKGROUND: Alpha-fetoprotein (AFP) is the most established tumor marker of hepatocellular carcinoma (HCC), but one of its limitations is non-specificity. Many studies have demonstrated that alpha-fetoprotein-L3 (AFP-L3) is more specific than AFP in the early diagnosis and prognosis of HCC. However, there is a lack of knowledge about the post-hepatectomy profiles of serum AFP and AFP-L3 values in HCC patients. To identify the profiles after surgical resection of HCC, we analyzed the correlation between the profiles and postoperative HCC recurrence or survival, and evaluated their utility in predicting postoperative therapeutic efficacy and prognosis. METHODS: From August 2003 to December 2004, 318 patients with positive serum AFP who had received surgical resections were enrolled in this study. Preoperative and postoperative serum AFP and AFP-L3 levels were measured simultaneously and regularly, and their postoperative profiles during a long term follow-up were recorded and summarized. RESULTS: A high ratio of AFP-L3 to total AFP was shown to correlate with pathologic features of aggressiveness. The overall 1-, 3-, and 5-year recurrence rates of the whole series were 28% 57%, and 84%, and the overall survival rates were 86%, 61% and 33%, respectively. The changes of serum AFP and AFP-L3 after hepatectomy for HCC were classified into 3 groups (group A: AFP-L3 undetectable; group B: AFP-L3 <10%; and group C: AFP-L3 >10%). Patients with positive postoperative AFP-L3had significantly earlier recurrence than those with negative results. The overall survival was significantly shorter in the positive groups than in the groups negative for postoperative AFP-L3.CONCLUSION: Post-hepatectomy changes in serum AFP and AFP-L3 levels occurred in three distinct patterns, which were closely correlated with HCC recurrence and patient survival with different prognostic values. | Xiao-Feng Zhang, Zheng-Feng Yin, Kui Wang, Zong-Qin Zhang, Hai-Hua Qian,Le-Hua ShiAuthor Affiliations: The 4th Department of Hepatic Surgery ,Molecular Oncology Laboratory , Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，6： | 19 |
| 5 | Combined invagination and duct-to-mucosa techniques with modifications:anew method of pancreaticojejunal anastomosis显示文摘BACKGROUND:Soft pancreatic texture and a small main pancreatic duct are thought to be the most significant risk factors for the occurrence of pancreatic fistula (PF),a common and serious complication after pancreaticoduodenectomy (PD).This is in part due to the technical difficulties of pancreaticojejunostomy (PJ) posed by a soft gland with a normal-sized duct.To deal with this problem,we developed a new anastomotic technique which combines the two most widely used techniques,namely,the invagination technique and the duct-to-mucosa technique,with a modification of the suture route and insertion of a temporary stent tube.METHODS:Between January 2003 and December 2009,ninetytwo consecutive patients underwent PD in which the new PJ technique was used.Charts and follow-up data of these patients were reviewed for operative details,early postoperative events,and outcomes at 6 months after the operation.PF was defined by the International Study Group on Pancreatic Fistula (ISGPF) guidelines and graded (A,B or C) according to the clinical procedures and outcome.RESULTS:In this group of 92 patients,there was only 1 early death from acute renal failure.PF was observed in 11 patients (12.0%),8 in grade A,1 in grade B,and 2 in grade C.For the 2 patients in grade C,PF was surgically managed.There were no early or late deaths attributable to PF.Six months after the operation,all of the patients were free of PJ-related symptoms except for 2,who were found to have steatorrhea.CONCLUSIONS:Our modified technique is simple and safe in PD.Present data suggest that this technique produces excellent early and medium-term results. | Bin Zhu,Li Geng,You-Gang Ma,Yong-Jie Zhang and Meng-Chao Wu Second Department of Biliary Surgery and Department of Special Treatment,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Shanghai 200438,China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，4： | 18 |
| 6 | Prognostic factors in the surgical treatment of caudate lobe hepatocellular carcinoma显示文摘AIM:To evaluate the short-and long-term outcomes of liver resection for caudate lobe hepatocellular carcinoma (HCC).METHODS:We retrospectively analyzed 114 consecutive patients with HCC,originating from the caudate lobe,who underwent resection between January 2001 and January 2007.Univariate and multivariate analyses were performed on several clinicopathologic variables to determine the factors affecting long-term outcome and intrahepatic recurrence.RESULTS:Overall mortality and morbidity were 0% and 18%,respectively.After a median follow-up of 31 mo (interquartile range,11-66 mo),tumor recurrence had occurred in 76 patients (66.7%).The 1-,3-,and 5-year disease-free survival rates were 65.7%,38.1%,and 18.4%,respectively.The 1-,3-,and 5-year overall survival rates were 76.1%,54.7%,and 31.8%,respectively.Univariate analysis showed that subsegmental location of the tumor (45.7% vs 16.2%,P=0.01),liver cirrhosis (12.3% vs 47.9%,P=0.03),surgical margin (18.5% vs 54.6%,P=0.04),vascular invasion (37.9% vs 23.2%,P=0.04) and extended caudate resection (42.1% vs 15.4%,P=0.04) were related to poorer long-term survival.Multivariate analysis showed that only subsegmental location of the tumor,liver cirrhosis and surgical margin were significant independent prognostic factors.CONCLUSION:Hepatectomy was an effective treatment for HCC in the caudate lobe.The subsegmental location of the tumor,liver cirrhosis and surgical margin affected long-term survival. | Peng Liu,Jia-Mei Yang,Wen-Yang Niu,Tong Kan,Feng Xie,Dian-Qi Li,Ye Wang,Yan-Ming Zhou,Department of Special Treatment and Liver Transplantation,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Shanghai 200438,China Peng Liu,Department of Hepatobiliary Surgery,Navy General Hospital,Beijing 100037,China | 2010 | World Journal of Gastroenterology2010，16，9： | 15 |
| 7 | Endoscopic Palliative Treatment for Malignant Obstructive Jaundice: A Report of 929 Cases显示文摘Objective:To review the experience with endoscopic palliative treatment for malignant obstructive jaundice (MOJ) in 929 patients. Methods:929 patients (598 males and 331 females) underwent 1215 endoscopic palliative drainages for MOJ in our hospital. Tumor obstruetion oeeurred in the distal common bile duet(CBD)(263 patients), the middle CBD (43),and the hilum(909).The mean bilirubin level was 284.3 μmol/L(range 26-810). Of the 1215 drainages, 599 were performed by stenting with plastic endoprosthesis, 385 by naso-biliary eatheterization, 167 by expandable metal stents,39 by combined drainage method,19 by tumor sphincterotomy and 6 by endoseopie fistulostomy. Results:The successful rate of endoscopic procedure was 94.3%. The jaundice symptom was improved in 81.8% of the patients with a significant reduetion of serum bilirubin in 64.7%. The complication after treatment occurred in 23 eases (2.6%), including eholangitis(23 eases), panereatitis (8 eases), and bleeding (one ease), and 3 eases were dead (0.2%).The median survival time of all patients was 14 months and life time analysis showed a life expectancy of 75.9%, 44.0% and 25.2% at 1, 2 and 3 years respectively. Conclusion:In the patients with MOJ seeondary to panereatobiliary malignancy, sueeessful endoscopic drainage provides adequate relief of biliary obstruetion and is associated with low morbidity and mortality. Enduseopie interventional treatment may be considered as an alternative of palliative biliary operation for the late stage of pancreatic and biliary malignaneies. | HU Bing, ZHOU Daiyun, GONG Biao, WANG Shuzhi, WU Mengchao Eastern Hepatohiliary Surgery Hospital, Second Military Medical University Shanghai 200438, China | 2002 | The Chinese-German Journal of Clinical Oncology2002，1，1： | 15 |
| 8 | Relationship between the imaging features and pathologic alteration in hepatoma of rats显示文摘AIM: The imaging features of MRI and DSA, using the modelsof implanted and induced hepatoma, were investigated in rats.METHODS: CBRH3 cancer cells were implanted for differentliver site of rat liver and the diethylnitrosoamine was givenorally to rats in order to induce liver cancer. Both experimentalgroups were detected by magnetic resonance imaging (MRI),digital subtraction angiography (DSA) and morphologic assay.RESULTS: Hypointensity on T1WI and homogenous highsignal intensity on T2WI in MRI, and ring-like abnormal stainon DSA were found in implanted cancer. Induced cancersappeared as homogeneous or heterogeneous hypointensityon T1WI (10 cases), and equal or slight high intensity onT2WI (8 cases), but some as hypointensity on T2WI (2 cases).CONCLUSION: The imaging features of implanted cancerswere similar to that of human liver metastases. Therefore, itcould serve as an experimental model of human liver metastatictumor. The imaging feature of induced cancers, whereas, weresimilar to that of human primary liver cancer. It could be useas an experimental model of human primary liver cancer. | Jia-He Yang Tian-Geng You Nan Li Qi-Jun Qian Ping Wang Zhen-Lin Yan Meng-Chao Wu Eastern Heptobiliary Hospital,225 Changhai Road,Shanghai 200433,China | 2003 | World Journal of Gastroenterology2003，9，1： | 13 |
| 9 | Surgical procedure and prognosis of hilar cholangiocarcinoma显示文摘BACKGROUND: Hilar cholangiocarcinoma has a low radi-cal resection rate and a poor long-term survival rate. In re-cent years, its prognosis has been improved with advance-ment of preoperative diagnostic techniques and surgicaltechniques. The aim of this study was to evaluate the prog-nostic factors of hilar cholangiocarcinoma and the relationsof surgical procedure to the prognosis of the carcinoma.METHODS: A retrospective cohort study was done in 198patients with hilar cholangiocarcinoma (117 men and 81women, aged from 27 to 81 years) , who had been admit-ted to this hospital from December 1997 to December 2002.Their symptoms were jaundice (94.5%), pruritus (56.6%)and abdominal pain (33.8%). Bismuth-Corlette classifica-tion showed type in 14 patients, type in 19, type in12, type in 15, type in 112, and unclassifiable typein 26. 144 patients underwent laparotomy and others re-ceived bile drainage endoscopically (including endoscopicretrograde biliary drainage (ERBD) or endoscopic metalbiliary endoprosthesis (EMBE) in 21 patients, endoscopicnose-biliary drainage (ENBD) in 31 or percutaneous trans-hepatic cholangiodrainage in 2. 120 patients (83.3%) re-ceived tumor resection including radical resection in 59 pa-tients (41.0%). Twenty-three patients underwent paunchedbiliary exploration and drainage.RESULTS: Cox’ s regression model analysis showed thatoccupation, preoperative total serum bilirubin level, opera-tive procedure and postoperative adjuvant radiation weresignificantly related to postoperative survival rate in con-trast to gender, age, choledocholithiasis, hepatitis, preope-rative serum CA19-9 level, Bismuth-Corlette type, his-topathologic grading and postoperative chemotherapy. Thesurvival of patients in groups of biliary drainage, palliativeresection and radical resection differed statistically and pro-longed in a descending order. No statistical difference wasfound between ERBD or EMBE group and palliative resec-tion group. So was between ERBD or EMBE group andbiliary drainage group, or between ENBD group and biliarydrainage group. The survival differed statistically betweenERBD or EMBE group and ENBD group.CONCLUSIONS: Operative procedure is the most impor-tant prognostic factor affecting the operative results of hilarcholangiocarcinoma. Radical resection is still the primarymeasure for a cure and long-term survival of the patients.For patients with irresectable hilar cholangiocarcinoma, noevidence has shown that the prognosis after treatment ofERBD or EMBE is poorer than that after laparotomy. | Bin Yi, Bai-He Zhang, Yong-Jie Zhang, Xiao-Qing Jiang, Bao-Hua Zhang,Wen-Long Yu, Qing-Bao Chen and Meng-Chao Wu Department of Biliary Surgery, Eastern HepatobiliarySurgery Hospital, Shanghai 200438 , China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，3： | 12 |
| 10 | Percutaneous cryoablation after chemoembolization of liver carcinoma: report of 34 cases显示文摘OBJECTIVE: To assess the indications, efficacy and clinical significance of percutaneous cryoablationfor liver carcinoma after transcatheter liver artery chemoembolization (TACE).METHODS: Thirty-four patients with histologically or clinically confirmed primary or metastaticcarcinomas were treated with TACE. One week to 1 month later, they were treated percutaneously underultrasound guidance using cryosurgical system in the period of July 2001 -June 2002. All patients werefollowed up to determine serum tumor marker, CT scans, MRI images or ultrasound images.RESULTS: This therapy was performed in 34 patients including 32 patients with Child A liver reserve, 2patients with Child B and no patient with Child C. There were 28 patients with primary liver cancer and6 patients with metastatic liver cancer. During the follow-up period (3 to 15 months), 41.1% patientswere recognized clinically cured because the serum tumor markers became normal, or CT scans and MRIimages revealed that the lesion became completely necrotic. 44.1% patients were recognized effectivelytreated.CONCLUSIONS: Percutaneous cryoablation combined with TACE is a choice of treatment for livercarcinoma. It is minimally invasive, safe and effective for those patients with liver cancer unsuitable forsurgery. | Guo-Jun Qian Han Chen Meng-Chao Wu the Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China | 2003 | Hepatobiliary & Pancreatic Diseases International2003，2，4： | 12 |
| 11 | Gene and protein expressions of p28^(GANK)in rat with liver regeneration显示文摘AIM: To observe the gene and protein expression changes of p28GANK in regenerating liver tissues, and to reveal the biological function of p28GANK on the regulation of liver regeneration.METHODS: One hundred and thirty two adult male Sprague-Dawley rats were selected, weighing 200-250 g,and divided randomly into sham operation (SO) group and partial hepatectomy (PH) group. Each group had eleven time points: 0, 2, 6, 12, 24, 30, 48, 72, 120, 168 and 240 h,six rats were in each time point. The rats were undergone 70 % PH under methoxyflurane anesthesia by resection of the anterior and left lateral lobes of the liver. SO was conducted by laparotomy plus slight mobilization of the liver without resection. Liver specimens were collected at the indicated time points after PH or SO. The expression level of p28GANK mRNA was determined by Northern blot as well as at protein level via immunohistochemical staining.The expressions of p28GANK mRNA in these tissues were analyzed by imaging analysis system of FLA-2000 FUJIFILM and one way analysis of variance. The protein expressions of p28GANK in these tissues were analyzed with Fromowitz'method and Rank sum test.RESULTS: The expression of p28GANK mRNA in bhe regenerating liver tissues possessed two transcripts, which were 1.5 kb and 1.0 kb. There was a significantly different expression patterns of p28GANK mRNA between SO and PH groups (P<0.01). The expression of p28GANK mRNA increased 2 h after PH, the peak time was 72 h (SO group: 163.83±1.4720; PH group: 510.5±17.0499, P<0.01). There was a significant difference in the 1.5 kb transcript, which decreased gradually after 72 hours. The protein expression of p28GANK was mainly in the cytoplasm of regenerating hepatocytes, and increased near the central region 24 h after PH, and became strongly positive at 48 h (+++, vs the other time points P<0.05),but decreased 72 h after PH.CONCLUSION: The expression of p28GANK mRNA increases in the early stage of rat liver regeneration, the protein expression of p28GANK is mainly in the cytoplasm of regenerating liver cells. It suggests that the gene of p28GANK may be an important regulatory and controlled factor involved in hepatocyte proliferation during liver regeneration. | Jian-Min Qin Xiao-Yong Fu Shen-Jing Li Shu-Qin Liu Jin-Zhang Zeng Xiu-Hua Qiu Meng-Chao Wu Hong-Yang Wang, International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai, 200438, China | 2003 | World Journal of Gastroenterology2003，9，11： | 12 |
| 12 | Use of acoustic intensity measurements in underwater acoustics (Modern state and prospects)显示文摘This paper discusses scalar and vector characteristics of underwater ambient noise which were explored by the authors and their colleagues in different regions of the Earth Ocean.The measurements presented were performed in 6 Hz - 1000 Hz band, at depths up to 1000 m and wind speeds less than 15 m/s. The following questions are addressed: ratio between edsotropic (coherent) and isotropic (diffusive) parts of ambient noise; noise energy transport and its cotmection with surface waves propagation direction; noise energy flow cancenation by opposite energy flow of signal; signal-to-noise ratio for combined receiver in dynamic noise field. It follows from the results presellted, that further development of underwater intensity measurements opens new opportunities for investigations in the fields of underwater acoustics and oceanology. | Vladimir A. Shchurov Marianna V. Kuyanova(Pacific Oceanological Institute, Far Eastern Branch of Russian Academg of Sciences Russia) | 1999 | Chinese Journal of Acoustics1999，18，4： | 9 |
| 13 | Expression of liver cancer associated gene HCCA3显示文摘AIM: To study and clone a novel liver cancer reisted gene,and to explore the molecular basis of liver cancer genesis.METHODS: Using mRNA differential display polymerasechain reaction (DDPCR), we investigated the difference of mRNA in human hepatocellular carcinoma (HCC) and paired surrounding liver tissues, and got a gene probe. By screening a human placenta cDNA library and genomic homologous extend, we obtained a full-length cDNA named HCCA3. We analyzed the expression of this novel gene in 42pairs of HCC and the surrounding liver tissues, and distribution in human normal tissues by means of Northern blot assay.RESULTS: A full-length cDNA of liver cancer associated gene HCCA3 has been submitted to the GeneBank nucleotide sequence databases ( Accession No. AF276707 ). The positive expression rate of this gene was 78.6% (33/42) in HCC tissues, and the clinical pathological data showed that the HCCA3 was closely associated with the invasion of tumor capsule ( P = 0.023) and adjacant small metastasis satellite nodules lesions ( P= 0.041). The HCCA3 was widely distributed in the human normal tissues, which was intensively expressed in lungs, brain and colon tissues,while lowly expressed in the liver tissues.CONCLUSION: A novel full-length cDNA was cloned and differentiated, which was highly expressed in liver cancer tissues. The high expression was closely related to the tumor invasiveness and metastasis, that may be the late heredited change in HCC genesis. | Zheng-Xu Wang~1 Gui-Fang Hu~1 Hong-Yang Wang~2 Meng-Chao Wu~2 1 Department of General Surgery,Chinese PEA General Hospital of Lanzhou Military Command,Lanzhou 730050,Gansu Province,China2 Eastern Hepatobilliary Surgical Hospital,Second Military Medical University,Shanghai 200438,China | 2001 | World Journal of Gastroenterology2001，7，6： | 9 |
| 14 | Expression of HBx protein in hepatitis B virus-infected intrahepatic cholangiocarcinoma显示文摘BACKGROUND: Hepatitis B virus (HBV) is an etiological factor of intrahepatic cholangiocarcinoma (ICC), but the pathogenic mechanisms remain unclear. This study aimed to investigate the expression and possible role of HBx, an HBV- encoded potentially oncogenic protein, in HBV-infected ICC. METHODS: Tissue samples were obtained from 54 specimens of HBV-infected ICC. Forty-four specimens were of peripheral type and 10 hilar type. Formalin-fixed, paraffin-embedded sections of the specimens were immunohistochemically stained for HBx and p53. RESULTS: HBx expression was found in 70.4% (38/54) of the specimens, and it was more frequently seen in the peripheral type than in the hilar type (79.5% vs 30.0%, P=0.002). All three well-differentiated ICCs expressed HBx, whereas 76.9% (30/39) moderately-differentiated and 41.7% (5/12) poorly-differentiated ICCs had HBx expression (P=0.033). Patients with HBx expression had a significantly higher prevalence of elevated serum alpha-fetoprotein (P=0.033). p53 protein expression was found in 18 of 54 cases (33.3%), and was not correlated with that of HBx. CONCLUSIONS: HBx may contribute to the pathogenesis of ICC, particularly the peripheral type. p53 abnormality may not play a significant role in HBx-mediated oncogenicity during ICC carcinogenesis. | Yan-Ming Zhou, Lu Cao, Bin Li, Xiu-Zhong Zhang, Zheng-Feng Yin Department of Hepato-Biliary-Pancreato-Vascular Surgery, First Affiliated Hospital of Xiamen University, Xiamen 361003, China Department of Molecular Oncology,Department of Pathology, Eastern Hepatobiliary Surgery Hospital,Second Military Medical University, Shanghai 200438, China | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，5： | 7 |
| 15 | Research on Relationships Between Built Environment and Criminal Behavior in Parking Areas: A Case Study on Pudong New Area, Shanghai | MAO Yuanyuan, PhD Candidate, School of Architecture & Urban Planning, Tongji University, Shanghai, P. R. China. DAI Shenzhi, Professor, School of Architecture & Urban Planning, Tongji University, Shanghai, P. R. China. DENG Tian, China Overseas Property Group Co., Ltd (Eastern China), Shanghai, P. R. China. XU Bin, Off icer, Shanghai Municipal Public Security Bureau Pudong Branch, Shanghai, P. R. China. ZHU Wei, PhD, Eindhoven University of Technology, Eindhoven, the Netherlands. DONG Hengping, Postgraduate student, School of Architecture & Urban Planning, Tongji University, Shanghai, P. R. China. | 2009 | China City Planning Review2009，18，2： | 7 |
| 16 | Hepatectomy in the treatment of very big primary liver cancer: report of 86 cases显示文摘Objective: To study the indications for resection ofvery big primary liver cancer and the operative re-sults.Methods: From January 1985 to June 1996, 86 pa-tients with very big primary liver cancer (≥15cm indiameter) underwent hepatectomy in our hospital.The volume of bleeding and blood transfusion wasrecorded during the operation. After the operation,the draining quantity from their abdominal cavities,and the days of transfusion and hospitalization wererecorded. The occurrence of complications and sur-vival time of the patient were followed up.Results: The postoperative mortality was 3.48% andthe occurrence rate of complications was 31.40%, whichwas significantly correlated with preoperative lowerlevel of serum albumin or the elevated γ-globulin lev-el and the amount of resected liver tissue. But their liverfunction before operation was fairly good, the 1-, 3-and 5-year survival rates after hepatectomy were58.2%, 35.7% and 17.64%.Conclusions: Patients with very big primary liver can-cer, should be subjected to hepatectomy if their liverfunction before operation are normal and the marginsare distinct between the tumor and liver tissues. Afterthe operation, other treatments are suitable for goodeffects. | Jia-Mei Yang Tong Kan Han Chen Meng-Chao Wu From the Eastern Hospital of Hepatobiliary Surgery, Second Military Medical University, Shanghai 200433 China | 2002 | Hepatobiliary & Pancreatic Diseases International2002，1，1： | 7 |
| 17 | E-selectin and its ligand-sLeX in the metastasis of hepatocellular carcinoma显示文摘Objective: To study the significance of E-selectin andits ligand-sLeX in the metastasis of hepatocellularcarcinoma (HCC).Methods: Flow cytometry and immunohistochemistrywere used to detect the expression of E-selectin and itsligand-sLeX in both HCC cell lines and human HCCtissues.Results: The positive rate of E-selectin in vascular en-dothelial cells adjacent to cancer was 67.9% (19/28). Theexpression of E-selectin in tumors accompanied withemboli or satellite foci was significantly higher than thatin tumors without emboli or satellite foci (P<0.05),and it was not related to tumor size, tumor capsule,AFP content, and the degree of differentiation. Thepositive expression of sLeX in SMMU-7721, PLF/PRF/5 and HepGⅡ cell lines was 7.03%, 63.35% and97.29% respectively. The positive cells of sLeX mainlydistributed in the margin of tumor tissues. The positiveexpression of sLeX in HCC cells in emboli or invasivetumor tissues was much higher than in primary foci.Conclusion: E-selectin and its ligand-sLeX are closelycorrelated with the metastasis of HCC. | Bao-Hua Zhang Han Chen Xiao-Ping Yao Wen-Ming Cong Meng-Chao Wu From the Eastern Hepatobiliary Surgery Hospital, Second Military Medical University of PLA, Shanghai 200438, China | 2002 | Hepatobiliary & Pancreatic Diseases International2002，1，1： | 6 |
| 18 | Modified Sugiura procedure for the management of 160 cirrhotic patients with portal hypertension显示文摘BACKGROUND: Portal hypertension is a common diseasewith a high mortality and serious effect on the life quality ofpatients. Presently, shunt and disconnection are commonlyused for surgical treatment of portal hypertension. The aimof this study was conducted to analyze the results of a modi-fied Sugiura procedure for the management of 160 cirrhoticpatients with portal hypertension.METHODS: The results of a modified Sugiura procedurefor the treatment of 160 cirrhotic patients with portal hyper-tension from January 1991 to July 2002 were retrospectivelyanalyzed.RESULTS: The operative mortality for the procedure waszero. Postoperative intra-abdominal bleeding was noted in2 patients, drowned lung in 1, pneumonia in 1, and splenicvenous thrombosis in 4. Of the 160 patients, 157 (98%)were followed up from 6 months to 11.5 years. Of the 157patients, only one died of hepatic coma 6 years after opera-tion, and 3 of rebleeding. The absolute and relative survivalrates were 97.5% (156/160) and 99% (159/160), respective-ly. The absolute and relative occurrence rates of hepatic co-ma were 2.5% (4/160) and 0.6% (1/157), respectively. Theabsolute and relative occurrence rates of rebleeding were3.8% (6/160) and 1.9% (3/157) , respectively. In 96 of 116Child B patients (82.8%), liver function improved frompreoperative class B to A 3 months after operation. Sixty-five patients were subjected to gastroscopy and 22 patients,esophageal barium photography 6 months after operation.Gastro-esophageal varices disappeared in 56 patients(64.4%, 56/87), obviously improved in 30 (34.5%, 30/87),and unchanged in 1 (1.2%, 1/87). The occurrence rate ofportal hypertensive gastropathy (PHG) was 13.9% (9/65).CONCLUSION: Our results showed that the modifiedSugiura procedure is effective in the treatment of portal hy-pertension, with a low rate of operative complication,bleeding recurrence, and hepatic coma. | You-Gang Ma, Xiao-Song Li, Jun Zhao, Han Chen and Meng-Chao Wu Department of Second Biliary Surgery, Eastern Hepa-tobiliary Hospital, Shanghai 200438, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，3： | 6 |
| 19 | Surgical therapy for hiliar cholangiocarcinonia:analysis of 198 cases显示文摘BACKGROUND: Carcinoma of the hepatic duct confluence is the most common site of bile duct malignancies. Although hilar cholangiocarcinoma has been characterized as a slow-growing and late metastasizing tumor, post-therapeutic prognosis has remained poor. The study was undertaken to analyze factors influencing the surgical curative effect of hilar cholangiocarcinoma. METHODS: A retrospective clinical analysis was made of 198 patients with hilar cholangiocarcinoma who had been surgically treated at our hospital from 1997 to 2002. Jaundice (94.5%, 187 patients), pruritus (56.6%, 112) and abdominal pain (33.8%, 67) were the main symptoms. According to the Bismuth-Corlette classification, there were 14 type Ⅰ patients, 19 type Ⅱ patients, 12 type Ⅲa patients, 15 type Ⅲb patients, 112 type Ⅳ patients, and 26 unclassified patients. 144 patiens received laparotomy, and 120 tumor resection including radical resection (59 patients) and palliative resection (61). Fifty-four patients were treated by endoscopic surgery and 16 patients by postoperative adjuvant radiation. RESULTS: Occupation, preoperative level of total serum bilirubin, operative procedure and postoperative adjuvant radiation affected postoperative survival of the patients. The postoperative suvivals of endoscopic nose-biliary drainage (ENBD) group, endoscopic retrograde biliary drainage (ERBD) or endoscopic metal biliary endoprosthesis (EMBE) group, biliary exploration and drainage group, palliative resection group and radical resection group differed (x2 =87.0489, P<0.01). CONCLUSION: Early diagnosis and radical resection are important to improve the prognosis of hilar cholangiocarcinoma. | Bai-He Zhang, Qing-Bao Cheng, Xiang-Ji Luo, Yong-Jie Zhang, Xiao-Qing Jiang, Bao-Hua Zhang, Bin Yi, Wen-Long Yu and Meng-Chao Wu Department of Biliary Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，2： | 5 |
| 20 | Effect of Sirpα1 on the expression of nuclear factor-kappa B in hepatocellular carcinoma显示文摘BACKGROUND:Signal regulatory protein alpha1(Sirpα1) is a member of Sirps families containing four immunoreceptor tyrosine-based inhibitory motifs(ITIMs) domains in the cytoplasm of and an activated substrate of receptor tyrosine kinase(RTK),that negatively regulates the RTK-dependent cell proliferating signal transduction pathway.Previously we found that Sirpα1 was closely associated with the occurrence and development of hepatocellular carcinoma(HCC)as well as liver regeneration.Since it is unclear about the regulatory mechanisms,we established the cell line transfected Sirpα1 gene and preliminarily clarified the mechanisms by which Sirpα1 negatively regulates the carcinogenesis and development of HCC. METHODS:Liver cancer Sk-Hep1 cell was respectively transfected with plasmids of pLXSN,pLXSN-Sirpα1 and pLXSN-Sirpα1Δ4Y 2 ,screened with the drug of G418(1200 μg/ml),and various transfected Sk-Hep1 cell lines were obtained.The protein expressions of P65,P50,IκBα,cyclin D1 and Fas in various Sk-Hep1 cell lines were determined by Western blotting,and P65 and P50 were localized by the immunofluorescence technique. RESULTS:Sirpα1 could significantly upregulate the protein expression of IκBα(vs.other cell lines,P<0.05) in the Sk-Hep1 cell,and downregulate the protein expressions of P65,P50 and cyclin D1(vs.other cell lines, P<0.05)in the Sk-Hep1 cell.P65 protein expression was mainly localized in the cytoplasm in the pLXSN Sk-Hep1 cell,and in the nucleus of the Sk-Hep1 cell with mutantSirpα1Δ4Y 2 ,but in nucleus of the Sk-Hep1 cell with wild Sirpα1.P50 protein expression was localized in the cytoplasm and nucleus of the pLXSN Sk-Hep1 cell,but in the nucleus of the Sk-Hep1 cell with wild Sirpα1 and mutant Sirpα1Δ4Y 2 plasmid. CONCLUSIONS:Sirpα1 might negatively regulate and control the abnormal proliferation of liver cancer cells by influencing the protein content and localization of nuclear factor-kappa B,then influence the expression of cyclins such as cyclin D1 in the signal transduction pathway.It may be one of the important mechanisms by which Sirpα1 negatively regulates the carcinogenesis and development of HCC. | Jian-Min Qin,Xing-Wang Wan,Jin-Zhang Zeng and Meng-Chao Wu Department of Hepatobiliary and Pancreatic Surgery,Beijing Chaoyang Hospital,Capital University of Medical Sciences,Beijing 100020,China and Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Shanghai 200438, China | 2007 | Hepatobiliary & Pancreatic Diseases International2007，6，3： | 5 |
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