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| 1 | 国际胃袖状切除专家共识:基于>12000手术病例的最佳操作指南显示文摘腹腔镜胃袖状切除术(1aparoscopic sleeve gastrectomy,LSG)系相对较新的减重手术方式。因其操作相对简单、治疗肥胖合并疾病及减重效果明显,逐步得以广泛接受。目前美国代谢与减重手术协会将其推荐为肥胖症治疗的单独标准术式。2011年3月25~26日, | 苏远涛 徐安安 朱江帆 Diaz AA Arvidsson D Baker RS Basso N Bellanger D Boza C El Mourad H France M Gagner M Galvao-Neto M Higa KD Himpens J Hutchinson CM Jacobs M Jorgensen JO Jossart G Lakdawala M Nguyen NT Nocca D Prager G Pomp A Ramos AC Rosenthal RJ Shah S Vix M Wittgrove A Zundel N | 2013 | 中国微创外科杂志2013，13，9： | 12 |
| 2 | IL28B polymorphism and cytomegalovirus predict response to treatment in Egyptian HCV type 4 patients显示文摘AIM:To test whether the status of positive cytomegalovirus(CMV) DNA detection adds to the predictive value of IL28B and to further categorize C/T allele carriers.METHODS:This study included 166 chronic hepatitis C(CHC) patients who received combined interferon and ribavirin therapy for 48 wk,84 spontaneous hepatitis C virus(HCV) resolvers who were positive for IgG anti-HCV antibody and negative for HCV RNA,and 100 healthy subjects who were negative for both HCV antibodies and RNA as controls.Genomic DNA from peripheral blood was used for IL28B rs.12979860 single nucleotide polymorphism(SNP) and CMV DNA detection.A 139 bp fragment containing IL28B SNP was amplified in all subjects by polymerase chain reaction using a specifically designed primer.Then the IL28B rs.12979860 SNP was detected by restriction fragment length polymorphism(RFLP) genotyping.The presence of CMV DNA was tested by amplification of the gB1 gene using nested polymerase chain reaction.The role of CMV and IL28B rs.12979860 SNP genotypes in determining the response rate to combined interferon therapy and clinical status of patients were statistically analyzed.RESULTS:Current data showed that 67% of patients carrying the IL28B 12979860 C/C allele had a sustained viral response(SVR) while the genotypes C/T and TT were associated with lower SVR rates,50% and 48%,respectively.SVR rates for the C/C allele were lower than other HCV genotypes and/or other populations.Genotype CC was associated with the response to interferon(P = 0.025).Genotype C/C was reduced from 48% in controls to 14% in CHC patients suggesting its protective role against progression to chronicity.The majority of spontaneously cleared subjects(86%) were C/C,confirming its protective role.The C/T allele was present in 71% of CHC patients compared with 38% of controls,so the use of IL28B SNP genotyping only in these patients may be of little value as a predictor of response.CMV reactivation occurred in 40% of CHC patients.Co-infection with CMV seriously diminished the response to interferon(IFN) therapy,with SVR rates in C/C genotypes 87.5% in CMV-negative patients and 12.5% in CMV-positive patients(P < 0.0001).SVR rates among C/T carriers were reduced to < 50% in patients with positive CMV DNA while the non-response rate doubled.These data indicate that a supplemental assay for CMV viremia adds to the prognostic value of IL28B genotyping.CONCLUSION:The results suggest that both genetic(i.e.,spontaneous) and therapeutic(IFN-based therapy) arms are complementary in the battle against HCV.CMV DNA testing may be of value to better predict the response to IFN,particularly in IL28B C/T carriers. | Mostafa K El Awady Noha G Bader El Din Ashraf Tabll Yaser El Hosary Ashraf O Abdel Aziz Hesham El Khayat Mohsen Salama Tawfeek H Abdelhafez | 2013 | World Journal of Gastroenterology2013，19，2： | 8 |
| 3 | Tumor necrosis factor-α-G308A polymorphism is associated with liver pathological changes in hepatitis C virus patients显示文摘AIM To investigate the association of tumor necrosis factor alpha(TNFα)-G308 A polymorphism with different liver pathological changes in treatment-na?ve Egyptian patients infected with hepatitis C virus(HCV) genotype 4.METHODS This study included 180 subjects,composed of 120 treatment-na?ve chronic HCV patients with different fibrosis grades(F0-F4) and 60 healthy controls. The TNFα-G308 A region was amplified by PCR and the different genotypes were detected by restriction fragment length polymorphism analysis. The TNFα protein was detected by enzyme-linked immunosorbent assay. The influence of different TNFα-G308 A genotypes on TNFα expression and liver disease progression were statistically analyzed. The OR and 95%CI were calculated to assess the relative risk confidence.RESULTS Current data showed that the TNFα-G308 A SNP frequency was significantly different between controls and HCV infected patients(P = 0.001). Both the AA genotype and A allele were significantly higher in late fibrosis patients(F2-F4,n = 60) than in early fibrosis patients(F0-F1,n = 60)(P = 0.05,0.04 respectively). Moreover,the GA or AA genotypes increased the TNFα serum level greater than the GG genotype(P = 0.002). The results showed a clear association between severe liver pathological conditions(inflammation,steatosis and fibrosis) and(GA + AA) genotypes(P = 0.035,0.03,0.04 respectively). The stepwise logistic regression analysis showed that the TNFα genotypes(GA + AA) were significantly associated with liver inflammation(OR = 3.776,95%CI: 1.399-10.194,P = 0.009),severe steatosis(OR = 4.49,95%CI: 1.441-14.0,P = 0.010) and fibrosis progression(OR = 2.84,95%CI: 1.080-7.472,P = 0.034). Also,the A allele was an independent risk factor for liver inflammation(P = 0.003),steatosis(P = 0.003) and fibrosis(P = 0.014). CONCLUSION TNFα SNP at nucleotide-308 represents an important genetic marker that can be used for the prognosis of different liver pathological changes in HCV infected | Noha G Bader El Din Sally Farouk Reem El-Shenawy Marwa K Ibrahim Reham M Dawood Mostafa M Elhady Ahmed M Salem Naglaa Zayed Ahmed Khairy Mostafa K El Awady | 2016 | World Journal of Gastroenterology2016，22，34： | 3 |
| 4 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 5 | Robotic transanal total mesorectal excision:Is the future now?显示文摘Total mesorectal excision(TME)is the standard surgical treatment for the curative radical resection of rectal cancers.Minimally invasive TME has been gaining ground favored by the continuous technological advancements.New procedures,such as transanal TME(TaTME),have been introduced to overcome some technical limitations,especially in low rectal tumors,obese patients,and/or narrow pelvis.The earliest TaTME reports showed promising results when compared with the conventional laparoscopic TME.However,recent publications raised concerns regarding the high rates of anastomotic leaks or local recurrences observed in national series.Robotic TaTME(R-TaTME)has been proposed as a novel technique incorporating the potential benefits of a perineal dissection together with precise control of the distal margins,and also offers all those advantages provided by the robotic technology in terms of improved precision and dexterity.Encouraging short-term results have been reported for R-TaTME,but further studies are needed to assess the real role of the new technique in the long-term oncological or functional outcomes.The present review aims to provide a general overview of R-TaTME by analyzing the body of the available literature,with a special focus on the potential benefits,harms,and future perspectives for this novel approach. | Juan Carlos Sebastián-Tomás Aleix Martínez-Pérez Elías Martínez-López Nicola de'Angelis Marcos Gómez Ruiz Eduardo García-Granero | 2021 | World Journal of Gastrointestinal Surgery2021，13，8： | 2 |
| 6 | Liquid-phase glycerol hydrogenolysis to 1,2-propanediol under nitrogen pressure using 2-propanol as hydrogen source显示文摘 | I. Gandarias P.L. Arias J. Requies M. El Doukkali M.B. Güemez | 2011 | Journal of Catalysis2011，，1： | 2 |
| 7 | Small inhibitors of ADP-ribosylation factor activation and function in mammalian cells显示文摘Small GTP-binding proteins of the ADP-ribosylation factor(Arf)family control various cell functional responses including protein transport and recycling between different cellular compartments,phagocytosis,proliferation,cytoskeletal remodelling,and migration.The activity of Arfs is tightly regulated.GTPase-activating proteins(GAPs)inactivate Arfs by stimulating GTP hydrolysis,and guanine nucleotide exchange factors(GEFs)stimulate the conversion of inactive GDP-bound Arf to the active GTP-bound conformation.There is increasing evidence that Arf small GTPases contribute to cancer growth and invasion.Increased expression of Arf6 and of Arf-GEPs,or deregulation Arf-GAP functions have been correlated with enhanced invasive capacity of tumor cells and metastasis.The spatiotemporal specificity of Arf activation is dictated by their GEFs that integrate various signals in stimulated cells.Brefeldin A(BFA),which inactivates a subset of Arf-GEFs,has been very useful for assessing the function of Golgi-localized Arfs.However,specific inhibitors to investigate the individual function of BFA-sensitive and insensitive ArfGEFs are lacking.In recent years,specific screens have been developed,and new inhibitors with improved selectivity and potency to study cell functional responses regulated by BFA-sensitive and BFA-insensitive Arf pathways have been identified.These inhibitors have been instrumental for our understanding of the spatiotemporal activation of Arf proteins in cells and demonstrate the feasibility of developing small molecules interfering with Arf activation to prevent tumor invasion and metastasis. | Sylvain G Bourgoin Mohammed-Amine El Azreq | 2012 | World Journal of Pharmacology2012，1，4： | 2 |
| 8 | A DNA vector-based RNAi technology to suppress gene expression in mammalian cells显示文摘 | Sui G Soohoo C Affar el B Gay F Shi Y Forrester W C Shi Y | | 0，，： | 2 |
| 9 | Effects of the HIV treatment drugs nevirapine and efavirenz on brain creatine kinase activity显示文摘 | Streck EL Scaini G Rezin GT | 2008 | Metab Brain Dis2008，23，4： | 1 |
| 10 | A typical features in pleomorphic adenoma clinicopathologic study and implications for management显示文摘 | Ethunandan M Witton R Hoffman G el al | 2006 | Int J Oral Maxillofae Surg2006，35，7： | 1 |
| 11 | lncreasedintra2abdoma 1 pressure causes bacterial translocation in rabbits 显示文摘 | Yagci G zeybek N Kaymakcloglu N el al | 2005 | China Med Assoc2005，68，4： | 1 |
| 12 | Ecosystem health:the concept, the ISEH, and the important tasks ahead显示文摘 | Rapport D J Bohm G Buckingham D el al | 1999 | Ecosystem Health1999，5，： | 1 |
| 13 | The shape and internal s tructure of the moon from the clementine mission 显示文摘 | D E Smith F G Lemoine at el | 1994 | Science1994，226，： | 1 |
| 14 | Long-term outcomes of coronary-artery bypass grafting versus stent implantation显示文摘 | Racz MJ Walford G | 2005 | N Engl J Med2005，352，21： | 1 |
| 15 | The proteasome is required for rapid initiation of death receptor-induced apoptoais显示文摘 | Sohn D Totxke G Essmann F el al | 2006 | Mol Cell Biol2006，26，5： | 1 |
| 16 | The sentinel fat pads: the relationship of the ROOF and SOOF to the tem- poral nerve in facial rejuvenation显示文摘 | SINGH D P FORTE A J APOSTOLII)ES J G el al | 2011 | Aesthet Surg J2011，31，1： | 1 |
| 17 | Biodiversityof bacterial ecosystems in traditional Egyptian Domiati cheese显示文摘 | El BARADEI G DELACROIX-BUCHET A OGIER J C | 2007 | ApplEnviron Microbiol2007，73，6： | 1 |
| 18 | A Multilevel Study of Leadership, Empowerment, and Performance in Teams 显示文摘 | Chen G Kirkman B L Kanfer R et el | 2007 | Journal of Applied Psychology2007，92，2： | 1 |
| 19 | Antioxidant activity of extracts from roasted wheat germ显示文摘 | Krings U El Saharty Y S Berger R G | 2000 | Food Chemistry2000，71，1： | 1 |
| 20 | Differential activation of extracellular signal-regulated protein kinase 1/2 and p38 mitogen activated-protein kinase by AT1 receptors in vascular smooth muscle cells from Wistar-Kyoto rats and spontaneously hypertensive rats显示文摘 | Touyz RM He G El Mabrouk M | 2001 | J Hypertens2001，19，32： | 1 |
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